Published in Annu Rev Genet on January 01, 1981
Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc Natl Acad Sci U S A (1985) 68.48
Directed mutagenesis of DNA cloned in filamentous phage: influence of hemimethylated GATC sites on marker recovery from restriction fragments. Nucleic Acids Res (1982) 5.72
A novel method for site-directed mutagenesis: its application to an eukaryotic tRNAPro gene promoter. EMBO J (1982) 4.67
Gap misrepair mutagenesis: efficient site-directed induction of transition, transversion, and frameshift mutations in vitro. Proc Natl Acad Sci U S A (1982) 2.78
Two-codon insertion mutagenesis of plasmid genes by using single-stranded hexameric oligonucleotides. Proc Natl Acad Sci U S A (1985) 2.50
The secD locus of E.coli codes for two membrane proteins required for protein export. EMBO J (1990) 2.40
Construction of infectious potato spindle tuber viroid cDNA clones. Nucleic Acids Res (1983) 2.21
Site-directed mutagenesis of the proposed catalytic amino acids of the Sindbis virus capsid protein autoprotease. J Virol (1990) 2.11
Effects of null mutations in the hexokinase genes of Saccharomyces cerevisiae on catabolite repression. Mol Cell Biol (1986) 2.06
Construction of mutants of Moloney murine leukemia virus by suppressor-linker insertional mutagenesis: positions of viable insertion mutations. Proc Natl Acad Sci U S A (1984) 2.05
Deletion loop mutagenesis: a novel method for the construction of point mutations using deletion mutants. Nucleic Acids Res (1982) 1.90
Genetic manipulation of Saccharomyces cerevisiae by use of the LYS2 gene. Mol Cell Biol (1986) 1.84
Local mutagenesis within deletion loops of DNA heteroduplexes. Proc Natl Acad Sci U S A (1982) 1.81
Use of cloned mtl genes of Escherichia coli to introduce mtl deletion mutations into the chromosome. J Bacteriol (1983) 1.72
Transcribed sequences of the Escherichia coli btuB gene control its expression and regulation by vitamin B12. Proc Natl Acad Sci U S A (1991) 1.67
The region of phage T4 genes 34, 33 and 59: primary structures and organization on the genome. Nucleic Acids Res (1986) 1.66
Construction of a "mutagenesis cartridge" for poliovirus genome-linked viral protein: isolation and characterization of viable and nonviable mutants. Proc Natl Acad Sci U S A (1988) 1.61
Cloning of the Escherichia coli release factor 2 gene. J Bacteriol (1984) 1.56
Genetic system for analyzing Escherichia coli thymidylate synthase. J Bacteriol (1984) 1.14
Site specific mutagenesis: insertion of single noncomplementary nucleotides at specified sites by error-directed DNA polymerization. Nucleic Acids Res (1984) 1.13
Mapping by multifragment cloning in vivo. Proc Natl Acad Sci U S A (1995) 1.01
Effect of amino acid substitutions on the catalytic and regulatory properties of aspartate transcarbamoylase. Proc Natl Acad Sci U S A (1986) 0.98
Thymidine kinase mutants obtained by random sequence selection. Proc Natl Acad Sci U S A (1993) 0.97
High-throughput DNA synthesis in a multichannel format. Nucleic Acids Res (1995) 0.93
What have we learned from the studies of two-state folders, and what are the unanswered questions about two-state protein folding? Phys Biol (2009) 0.91
Increased expression of a cloned gene by local mutagenesis of its promoter and ribosome binding site. Nucleic Acids Res (1983) 0.84
Effects of amino acid substitutions in the promoter -10 binding region of the sigma A factor on growth of Bacillus subtilis. J Bacteriol (1993) 0.80
Solid phase-supported thymine dimers for the construction of dimer-containing DNA by combined chemical and enzymatic synthesis: a potentially general method for the efficient incorporation of modified nucleotides into DNA. Nucleic Acids Res (1997) 0.79
Crystallographic structure of an active, sequence-engineered ribonuclease. Proc Natl Acad Sci U S A (1985) 0.77
Identification of two distinct regulatory regions adjacent to the human beta-interferon gene. Cell (1983) 17.60
Restriction endonucleases in the analysis and restructuring of dna molecules. Annu Rev Biochem (1975) 11.76
Specific cleavage of simian virus 40 DNA by restriction endonuclease of Hemophilus influenzae. Proc Natl Acad Sci U S A (1971) 10.06
Studies of simian virus 40 DNA. VII. A cleavage map of the SV40 genome. J Mol Biol (1973) 9.30
Letter: A suggested nomenclature for bacterial host modification and restriction systems and their enzymes. J Mol Biol (1973) 9.17
Local mutagenesis: a method for generating viral mutants with base substitutions in preselected regions of the viral genome. Proc Natl Acad Sci U S A (1978) 8.36
A gene activated by growth factors is related to the oncogene v-jun. Proc Natl Acad Sci U S A (1988) 7.47
Expression of a set of growth-related immediate early genes in BALB/c 3T3 cells: coordinate regulation with c-fos or c-myc. Proc Natl Acad Sci U S A (1987) 7.38
Lethal disruption of the yeast actin gene by integrative DNA transformation. Science (1982) 6.88
Isolation of mutants of an animal virus in bacteria. Science (1980) 6.81
Bidirectional replication of Simian Virus 40 DNA. Proc Natl Acad Sci U S A (1972) 6.79
Identification of a set of genes expressed during the G0/G1 transition of cultured mouse cells. EMBO J (1985) 6.31
DNA binding activities of three murine Jun proteins: stimulation by Fos. Cell (1988) 6.13
Deletion mutants of simian virus 40 generated by enzymatic excision of DNA segments from the viral genome. J Mol Biol (1974) 5.47
Protein interaction cloning in yeast: identification of mammalian proteins that react with the leucine zipper of Jun. Proc Natl Acad Sci U S A (1992) 5.28
A gene activated in mouse 3T3 cells by serum growth factors encodes a protein with "zinc finger" sequences. Proc Natl Acad Sci U S A (1988) 5.25
A map of simian virus 40 transcription sites expressed in productively infected cells. J Mol Biol (1973) 5.02
Identification of simian virus 40 protein A. J Virol (1977) 4.93
Posttranscriptional selection of simian virus 40-specific RNA. J Virol (1975) 4.87
Denatured states of proteins. Annu Rev Biochem (1991) 4.86
A map of temperature-sensitive mutants of simian virus 40. Virology (1975) 4.77
Bovine papillomavirus vector that propagates as a plasmid in both mouse and bacterial cells. Proc Natl Acad Sci U S A (1982) 4.69
DNA binding site of the growth factor-inducible protein Zif268. Proc Natl Acad Sci U S A (1989) 4.52
Specific origin in SV40 DNA replication. Nat New Biol (1972) 4.39
The isolation of simian virus 40 variants with specifically altered genomes. Proc Natl Acad Sci U S A (1974) 4.35
Mapping temperature-sensitive mutants of simian virus 40: rescue of mutants by fragments of viral DNA. Virology (1974) 4.30
Mutational analysis of the simian virus 40 replicon: pseudorevertants of mutants with a defective replication origin. Proc Natl Acad Sci U S A (1979) 4.15
A gene inducible by serum growth factors encodes a member of the steroid and thyroid hormone receptor superfamily. Proc Natl Acad Sci U S A (1988) 3.77
Cold-sensitive regulatory mutants of simian virus 40. J Mol Biol (1980) 3.77
Regulatory mutants of simian virus 40: constructed mutants with base substitutions at the origin of DNA replication. J Mol Biol (1979) 3.75
Mutational analysis of simian virus 40 T antigen: isolation and characterization of mutants with deletions in the T-antigen gene. Mol Cell Biol (1983) 3.67
jun-D: a third member of the jun gene family. Proc Natl Acad Sci U S A (1989) 3.47
Construction and genetic characterization of temperature-sensitive mutant alleles of the yeast actin gene. Proc Natl Acad Sci U S A (1984) 3.47
Letter: Potential biohazards of recombinant DNA molecules. Science (1974) 3.45
The genome of simian virus 40. Adv Virus Res (1977) 3.42
A genetic system for analysis of staphylococcal nuclease. Gene (1983) 3.41
Growth-related changes in specific mRNAs of cultured mouse cells. Proc Natl Acad Sci U S A (1983) 3.35
Regulatory mutants of simian virus 40. Effect of mutations at a T antigen binding site on DNA replication and expression of viral genes. J Mol Biol (1982) 3.22
Translation of open reading frame E5 of bovine papillomavirus is required for its transforming activity. Proc Natl Acad Sci U S A (1986) 3.17
A naturally occurring truncated form of FosB that inhibits Fos/Jun transcriptional activity. Cell (1991) 3.12
Biochemical activities of T-antigen proteins encoded by simian virus 40 A gene deletion mutants. Mol Cell Biol (1983) 3.06
Growth factor-induced delayed early response genes. Mol Cell Biol (1992) 3.03
Induction of protooncogene c-jun by serum growth factors. Proc Natl Acad Sci U S A (1988) 2.92
Activation of the platelet-derived growth factor receptor by the bovine papillomavirus E5 transforming protein. EMBO J (1991) 2.87
Repression of human papillomavirus oncogenes in HeLa cervical carcinoma cells causes the orderly reactivation of dormant tumor suppressor pathways. Proc Natl Acad Sci U S A (2000) 2.84
Gap misrepair mutagenesis: efficient site-directed induction of transition, transversion, and frameshift mutations in vitro. Proc Natl Acad Sci U S A (1982) 2.78
Isolation of cloned cDNA encoding mammalian ornithine decarboxylase. Proc Natl Acad Sci U S A (1984) 2.64
Nucleotide sequence of murine ornithine decarboxylase mRNA. Proc Natl Acad Sci U S A (1985) 2.57
The evolution of new species of viral DNA during serial passage of simian virus 40 at high multiplicity. Virology (1973) 2.53
44-amino-acid E5 transforming protein of bovine papillomavirus requires a hydrophobic core and specific carboxyl-terminal amino acids. Mol Cell Biol (1988) 2.50
An Id-related helix-loop-helix protein encoded by a growth factor-inducible gene. Proc Natl Acad Sci U S A (1991) 2.44
Cooperative transcriptional activity of Jun and Stat3 beta, a short form of Stat3. Proc Natl Acad Sci U S A (1995) 2.42
A growth factor-inducible nuclear protein with a novel cysteine/histidine repetitive sequence. J Biol Chem (1990) 2.39
Characterization of long-range structure in the denatured state of staphylococcal nuclease. II. Distance restraints from paramagnetic relaxation and calculation of an ensemble of structures. J Mol Biol (1997) 2.38
Evolutionary variants of simian virus 40: Nucleotide sequence of a conserved SV40 DNA segment containing the origin of viral DNA replication as an inverted repetition. J Mol Biol (1978) 2.32
rheb, a growth factor- and synaptic activity-regulated gene, encodes a novel Ras-related protein. J Biol Chem (1994) 2.25
Characterization of long-range structure in the denatured state of staphylococcal nuclease. I. Paramagnetic relaxation enhancement by nitroxide spin labels. J Mol Biol (1997) 2.24
Genetic and biochemical definition of the bovine papillomavirus E5 transforming protein. EMBO J (1987) 2.23
Nonsense mutation in open reading frame E2 of bovine papillomavirus DNA. J Virol (1986) 2.22
Binding of an SV40 T antigen-related protein to the DNA of SV40 regulatory mutants. Nature (1981) 2.16
Segment-directed mutagenesis: construction in vitro of point mutations limited to a small predetermined region of a circular DNA molecule. Proc Natl Acad Sci U S A (1980) 2.10
Genetic analysis of staphylococcal nuclease: identification of three intragenic "global" suppressors of nuclease-minus mutations. Genetics (1985) 2.08
Directed mutagenesis with sodium bisulfite. Methods Enzymol (1983) 2.07
Strategies and applications of in vitro mutagenesis. Science (1985) 2.07
Purification of simian virus 40 large T antigen by immunoaffinity chromatography. J Virol (1985) 2.01
Evolutionary variants of simian virus 40: Cellular DNA sequences and sequences at recombinant joints of substituted variants. J Mol Biol (1978) 1.96
A colinear map relating the simian virus 40 (SV40) DNA segments of six adenovirus-SV40 hybrids to the DNA fragments produced by restriction endonuclease cleavage of SV40 DNA. Proc Natl Acad Sci U S A (1974) 1.94
Rapid induction of senescence in human cervical carcinoma cells. Proc Natl Acad Sci U S A (2000) 1.93
Inhibition of cervical carcinoma cell line proliferation by the introduction of a bovine papillomavirus regulatory gene. J Virol (1993) 1.92
Stable association between the bovine papillomavirus E5 transforming protein and activated platelet-derived growth factor receptor in transformed mouse cells. Proc Natl Acad Sci U S A (1992) 1.89
Cell-free protein synthesis directed by coliphage MS2 RNA: synthesis of intact viral coat protein and other products. J Mol Biol (1965) 1.89
Kinetic and ultraviolet spectroscopic studies of active-site mutants of delta 5-3-ketosteroid isomerase. Biochemistry (1989) 1.85
Nucleotide sequence of a growth-related mRNA encoding a member of the prolactin-growth hormone family. Proc Natl Acad Sci U S A (1984) 1.82
Studies of SV40 DNA. VI. Cleavage of SV40 DNA by restriction endonuclease from Hemophilus parainfluenzae. Virology (1973) 1.81
Local mutagenesis within deletion loops of DNA heteroduplexes. Proc Natl Acad Sci U S A (1982) 1.81
Studies of SV40 DNA. 3. Differences in DNA from various strains of SV40. J Mol Biol (1972) 1.79
Functional serum response elements upstream of the growth factor-inducible gene zif268. Mol Cell Biol (1989) 1.78
The basic region of Fos mediates specific DNA binding. EMBO J (1989) 1.75
Contributions of the polar, uncharged amino acids to the stability of staphylococcal nuclease: evidence for mutational effects on the free energy of the denatured state. Biochemistry (1992) 1.75
Truncated staphylococcal nuclease is compact but disordered. Proc Natl Acad Sci U S A (1992) 1.72
The nucleic acid of an SV40-like virus isolated from a patient with progressive multifocal leukoencephalopathy. Virology (1973) 1.71
The B/C gene of simian virus 40. Virology (1976) 1.67
Mapping the genes of simian virus 40. Cold Spring Harb Symp Quant Biol (1975) 1.66
Simian virus 40 mutant T antigens with relaxed specificity for the nucleotide sequence at the viral DNA origin of replication. J Virol (1984) 1.65
In a staphylococcal nuclease mutant the side-chain of a lysine replacing valine 66 is fully buried in the hydrophobic core. J Mol Biol (1991) 1.64
Efficient transactivation and morphologic transformation by bovine papillomavirus genes expressed from a bovine papillomavirus/simian virus 40 recombinant virus. Proc Natl Acad Sci U S A (1988) 1.62
Tumorigenic transformation of murine keratinocytes by the E5 genes of bovine papillomavirus type 1 and human papillomavirus type 16. J Virol (1991) 1.62
Mutants of simian virus 40 with base substitutions at the origin of DNA replication. Cold Spring Harb Symp Quant Biol (1979) 1.58
In vitro activation of Stat3 by epidermal growth factor receptor kinase. Proc Natl Acad Sci U S A (1996) 1.58
Structure and dynamics of a denatured 131-residue fragment of staphylococcal nuclease: a heteronuclear NMR study. Biochemistry (1994) 1.55
Transforming activity of a 16-amino-acid segment of the bovine papillomavirus E5 protein linked to random sequences of hydrophobic amino acids. J Virol (1989) 1.54
Open reading frames E6 and E7 of bovine papillomavirus type 1 are both required for full transformation of mouse C127 cells. J Virol (1989) 1.52
Multivalent DNA-binding properties of the HMG-1 proteins. Proc Natl Acad Sci U S A (1996) 1.51
A transcriptional map of the SV40 genome in transformed cell lines. Virology (1975) 1.49