Mapping the active site of papain with the aid of peptide substrates and inhibitors.

L-trans-Epoxysuccinyl-leucylamido(4-guanidino)butane (E-64) and its analogues as inhibitors of cysteine proteinases including cathepsins B, H and L. Biochem J (1982) 5.67

SCH 503034, a mechanism-based inhibitor of hepatitis C virus NS3 protease, suppresses polyprotein maturation and enhances the antiviral activity of alpha interferon in replicon cells. Antimicrob Agents Chemother (2006) 2.75

Cleavage site analysis in picornaviral polyproteins: discovering cellular targets by neural networks. Protein Sci (1996) 2.12

A viral cleavage site cassette: identification of amino acid sequences required for tobacco etch virus polyprotein processing. Proc Natl Acad Sci U S A (1988) 2.00

Purification and characterization of poliovirus polypeptide 3CD, a proteinase and a precursor for RNA polymerase. J Virol (1992) 1.90

Proteolytic processing of ovalbumin and beta-galactosidase by the proteasome to a yield antigenic peptides. J Immunol (1994) 1.85

The 5' end of the equine arteritis virus replicase gene encodes a papainlike cysteine protease. J Virol (1992) 1.61

Differences in the interaction of the catalytic groups of the active centres of actinidin and papain. Rapid purification of fully active actinidin by covalent chromatography and characterization of its active centre by use of two-protonic-state reactivity probes. Biochem J (1981) 1.60

Kinetic specificity in papain-catalysed hydrolyses. Biochem J (1971) 1.60

Drug resistance in HIV-1 protease: Flexibility-assisted mechanism of compensatory mutations. Protein Sci (2002) 1.54

Kinetics of the hydrolysis of N-benzoyl-L-serine methyl ester catalysed by bromelain and by papain. Analysis of modifier mechanisms by lattice nomography, computational methods of parameter evaluation for substrate-activated catalyses and consequences of postulated non-productive binding in bromelain- and papain-catalysed hydrolyses. Biochem J (1974) 1.47

Inhibition of metallo-beta-lactamases by a series of mercaptoacetic acid thiol ester derivatives. Antimicrob Agents Chemother (1997) 1.40

A protease responsible for post-translational cleavage of a conserved Asn-Gly linkage in glycinin, the major seed storage protein of soybean. Proc Natl Acad Sci U S A (1992) 1.40

Supracrystallographic resolution of interactions contributing to enzyme catalysis by use of natural structural variants and reactivity-probe kinetics. Biochem J (1988) 1.35

Substrate determinants for cleavage in cis and in trans by the hepatitis C virus NS3 proteinase. J Virol (1995) 1.31

Evidence that binding to the s2-subsite of papain may be coupled with catalytically relevant structural change involving the cysteine-25-histidine-159 diad. Kinetics of the reaction of papain with a two-protonic-state reactivity probe containing a hydrophobic side chain. Biochem J (1979) 1.31

Determinants of substrate recognition by poliovirus 2A proteinase. J Virol (1992) 1.29

Crystal structure of human dipeptidyl peptidase IV in complex with a decapeptide reveals details on substrate specificity and tetrahedral intermediate formation. Protein Sci (2004) 1.23

Consequences of molecular recognition in the S1-S2 intersubsite region of papain for catalytic-site chemistry. Change in pH-dependence characteristics and generation of an inverse solvent kinetic isotope effect by introduction of a P1-P2 amide bond into a two-protonic-state reactivity probe. Biochem J (1988) 1.22

A convenient method of preparation of high-activity urease from Canavalia ensiformis by covalent chromatography and an investigation of its thiol groups with 2,2'-dipyridyl disulphide as a thiol titrant and reactivity probe. Biochem J (1976) 1.19

Substrate-derived two-protonic-state electrophiles as sensitive kinetic specificity probes for cysteine proteinases. Activation of 2-pyridyl disulphides by hydrogen-bonding. Biochem J (1987) 1.17

Enterovirus 71 and coxsackievirus A16 3C proteases: binding to rupintrivir and their substrates and anti-hand, foot, and mouth disease virus drug design. J Virol (2011) 1.16

Action of human liver cathepsin B on the oxidized insulin B chain. Biochem J (1983) 1.11

Deletion of PREPL, a gene encoding a putative serine oligopeptidase, in patients with hypotonia-cystinuria syndrome. Am J Hum Genet (2005) 1.11

Inhibition of proteolytic activity of poliovirus and rhinovirus 2A proteinases by elastase-specific inhibitors. J Virol (1993) 1.10

The activities of 'Pz-peptidase' and 'endopeptidase 24.15' are due to a single enzyme. Biochem J (1989) 1.09

Purification and characterization of two forms of a high-molecular-weight cysteine proteinase (porphypain) from Porphyromonas gingivalis. J Bacteriol (1994) 1.09

A rapid method for determination of endoproteinase substrate specificity: specificity of the 3C proteinase from hepatitis A virus. Proc Natl Acad Sci U S A (1991) 1.07

Identification of signalling and non-signalling binding contributions to enzyme reactivity. Alternative combinations of binding interactions provide for change in transition-state geometry in reactions of papain. Biochem J (1989) 1.04

Molecular dynamics simulations of the catalytic pathway of a cysteine protease: a combined QM/MM study of human cathepsin K. J Am Chem Soc (2007) 1.04

Crystal structure of papain-E64-c complex. Binding diversity of E64-c to papain S2 and S3 subsites. Biochem J (1992) 1.02

A kinetic and fluorimetric investigation of papain modified at tryptophan-69 and -177 by N-bromosuccinimide. Biochem J (1974) 1.01

In vitro translation and processing of cathepsin B of Schistosoma mansoni. EMBO J (1990) 0.96

Coronavirus 3CLpro proteinase cleavage sites: possible relevance to SARS virus pathology. BMC Bioinformatics (2004) 0.95

Substrate specificity and mode of action of a cellulase from Aspergillus niger. Biochem J (1978) 0.93

Studies on the extended active sites of acid proteinases. Proc Natl Acad Sci U S A (1974) 0.93

Bioinformatic analysis of functional differences between the immunoproteasome and the constitutive proteasome. Immunogenetics (2003) 0.93

The interplay of electrostatic fields and binding interactions determining catalytic-site reactivity in actinidin. A possible origin of differences in the behaviour of actinidin and papain. Biochem J (1989) 0.92

Probing the specificity of cysteine proteinases at subsites remote from the active site: analysis of P4, P3, P2' and P3' variations in extended substrates. Biochem J (2000) 0.92

A model to explain the pH-dependent specificity of cathepsin B-catalysed hydrolyses. Biochem J (1991) 0.90

A re-appraisal of the structural basis of stereochemical recognition in papain. Insensitivity of binding-site-catalytic-site signalling to P2-chirality in a time-dependent inhibition. Biochem J (1990) 0.90

Evidence for association-activation effects in reactions of papain from studies on its reactivity towards isomeric two-protonic-state reactivity probes. Biochem J (1979) 0.89

Enzyme-substrate interactions in the hydrolysis of peptides by cathepsins B and H from rat liver. Biochem J (1987) 0.88

Autocatalytic cleavage within classical swine fever virus NS3 leads to a functional separation of protease and helicase. J Virol (2013) 0.87

Factors that govern the specificity of transglutaminase-catalysed modification of proteins and peptides. Biochem J (1992) 0.87

The specificity of the S1' subsite of papain. Biochem J (1974) 0.87

Genetic selection of poliovirus 2Apro-binding peptides. J Virol (1999) 0.87

Effects of secondary binding by activator and inhibitor peptides on covalent intermediates of pig pepsin. Biochem J (1976) 0.86

Acyl intermediates in penicillopepsin-catalysed reactions and a discussion of the mechanism of action of pepsins. Biochem J (1975) 0.86

High throughput substrate phage display for protease profiling. Methods Mol Biol (2009) 0.86

Residue L143 of the foot-and-mouth disease virus leader proteinase is a determinant of cleavage specificity. J Virol (2008) 0.85

Characterization of the active site of human multicatalytic proteinase. Biochem J (1990) 0.85

Substrate specificity of barley cysteine endoproteases EP-A and EP-B. Plant Physiol (1998) 0.85

Dependence of the P2-S2 stereochemical selectivity of papain on the nature of the catalytic-site chemistry. Quantification of selectivity in the catalysed hydrolysis of the enantiomeric N-acetylphenylalanylglycine 4-nitroanilides. Biochem J (1990) 0.85

Context-dependent resistance to proteolysis of intrinsically disordered proteins. Protein Sci (2011) 0.84

Acyl and amino intermediates in reactions catalysed by pig pepsin. Analysis of transpeptidation products. Biochem J (1976) 0.82

Limited proteolysis of ribonuclease A with thermolysin in trifluoroethanol. Protein Sci (1997) 0.82

In Silico screening on the three-dimensional model of the Plasmodium vivax SUB1 protease leads to the validation of a novel anti-parasite compound. J Biol Chem (2013) 0.81

Demonstration that 1-trans-epoxysuccinyl-L-leucylamido-(4-guanidino) butane (E-64) is one of the most effective low Mr inhibitors of trypsin-catalysed hydrolysis. Characterization by kinetic analysis and by energy minimization and molecular dynamics simulation of the E-64-beta-trypsin complex. Biochem J (1996) 0.81

Chymopapain A. Purification and investigation by covalent chromatography and characterization by two-protonic-state reactivity-probe kinetics, steady-state kinetics and resonance Raman spectroscopy of some dithioacyl derivatives. Biochem J (1986) 0.81

An antiviral peptide inhibitor that is active against picornavirus 2A proteinases but not cellular caspases. J Virol (2006) 0.80

Recognition of Lys48-Linked Di-ubiquitin and Deubiquitinating Activities of the SARS Coronavirus Papain-like Protease. Mol Cell (2016) 0.80

The interaction of alpha-N-(p-toluenesulphonyl)-p-guanidino-L-phenylalanine methyl ester with thrombin and trypsin. Biochem J (1978) 0.80

Benzyloxycarbonylphenylalanylcitrulline p-nitroanilide as a substrate for papain and other plant cysteine proteinases. Biochem J (1984) 0.80

The structural origins of the unusual specificities observed in the isolation of chymopapain M and actinidin by covalent chromatography and the lack of inhibition of chymopapain M by cystatin. Biochem J (1995) 0.79

The structure and mechanism of stem bromelain. Evaluation of the homogeneity of purified stem bromelain, determination of the molecular weight and kinetic analysis of the bromelain-catalysed hydrolysis of N-benzyloxycarbonyl-L-phenylalanyl-L-serine methyl ester. Biochem J (1974) 0.79

Telaprevir/boceprevir era: from bench to bed and back. World J Gastroenterol (2012) 0.79

Peptide Backbone Composition and Protease Susceptibility: Impact of Modification Type, Position, and Tandem Substitution. Chembiochem (2015) 0.78

Subsite differences between the active centres of papaya peptidase A and papain as revealed by affinity chromatography. Purification of papaya peptidase A by ionic-strength-dependent affinity adsorption on an immobilized peptide inhibitor of papain. Biochem J (1984) 0.78

Tri-domain bifunctional inhibitor of metallocarboxypeptidases A and serine proteases isolated from marine annelid Sabellastarte magnifica. J Biol Chem (2012) 0.76

Chemical synthesis and papain-catalysed hydrolysis of N-alpha-benzyloxycarbonyl-L-lysine p-nitroanilide. Biochem J (1985) 0.76

Characterization of the protease domain of Rice tungro bacilliform virus responsible for the processing of the capsid protein from the polyprotein. Virol J (2005) 0.75

Analysis of the Specificity and Biochemical Characterization of Metalloproteases Isolated from Eupenicillium javanicum Using Fluorescence Resonance Energy Transfer Peptides. Front Microbiol (2017) 0.75

Structure of a switchable subtilisin complexed with a substrate and with the activator azide. Biochemistry (2009) 0.75

Monitoring proteolytic processing events by quantitative mass spectrometry. Expert Rev Proteomics (2017) 0.75

On the size of the active site in proteases. I. Papain. Biochem Biophys Res Commun (1967) 18.32

Maturation of the immune response in germinal centers. Cell (1991) 5.68

Why doesn't audit work? BMJ (1998) 5.63

Private company wins rights to Icelandic gene database. BMJ (1999) 4.04

Biologically and chemically pure mRNA coding for a mouse immunoglobulin L-chain prepared with the aid of antibodies and immobilized oligothymidine. Proc Natl Acad Sci U S A (1973) 4.02

On the active site of proteases. 3. Mapping the active site of papain; specific peptide inhibitors of papain. Biochem Biophys Res Commun (1968) 3.10

The localization of antigen in relation to specific antibody-producing cells. I. Use of a synthetic polypeptide [(T,G)-A--L] labelled with iodine-125. Immunology (1966) 3.09

Dysfunction of early-stage visual processing in schizophrenia. Am J Psychiatry (2001) 2.80

Partial amino acid sequence of the precursor of immunoglobulin light chain programmed by messenger RNA in vitro. Science (1975) 2.48

Silicon nanowire-based solar cells on glass: synthesis, optical properties, and cell parameters. Nano Lett (2009) 2.41

Detection of Herpes simplex virus DNA by real-time PCR. J Clin Microbiol (2000) 2.29

Homozygous protein C deficiency manifested by massive venous thrombosis in the newborn. N Engl J Med (1984) 2.26

Reduction of diagnostic window by new fourth-generation human immunodeficiency virus screening assays. J Clin Microbiol (1998) 2.25

Conformational studies of poly-L-alanine in water. Biopolymers (1968) 2.02

Position-dependent accommodative shift of retropupillary fixated iris-claw lenses. Graefes Arch Clin Exp Ophthalmol (2012) 2.00

The interfascicular nerve-grafting of the median and ulnar nerves. J Bone Joint Surg Am (1972) 1.82

Primary structures of N-terminal extra peptide segments linked to the variable and constant regions of immunoglobulin light chain precursors: implications on the organization and controlled expression of immunoglobulin genes. Biochemistry (1978) 1.82

Invasion of respiratory epithelial cells by Burkholderia (Pseudomonas) cepacia. Infect Immun (1996) 1.82

Pilonidal sinus disease. J Visc Surg (2013) 1.76

Amino acid sequence of the NH2-terminal extra piece segments of the precursors of mouse immunoglobulin lambda1-type and kappa-type light chains. Proc Natl Acad Sci U S A (1977) 1.75

Persistence of pedicle blood flow up to 10 years after free musculocutaneous tissue transfer. Plast Reconstr Surg (1998) 1.67

On estimating recessive frequencies from truncated samples. Biometrics (1967) 1.62

Matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry as a tool for rapid diagnosis of potentially toxigenic Corynebacterium species in the laboratory management of diphtheria-associated bacteria. Euro Surveill (2010) 1.60

Right colonic involvement is associated with severe forms of ischemic colitis and occurs frequently in patients with chronic renal failure requiring hemodialysis. Am J Gastroenterol (2000) 1.59

Neonatal urinary uric acid/creatinine [correction of ceratinine] ratio as an additional marker of perinatal asphyxia. Eur J Pediatr (1995) 1.59

Cystic retroperitoneal lymphangiomas in adults. Eur J Surg Oncol (1997) 1.59

Stability of the Astronomical Frequencies Over the Earth's History for Paleoclimate Studies. Science (1992) 1.54

Marked hydrophobicity of the NH2-terminal extra piece of immunoglobulin light-chain precursors: possible physiological functions of the extra piece. Proc Natl Acad Sci U S A (1976) 1.54

Chicken immunoglobulin gamma-heavy chains: limited VH gene repertoire, combinatorial diversification by D gene segments and evolution of the heavy chain locus. EMBO J (1988) 1.53

The purification of papain by affinity chromatography. Eur J Biochem (1970) 1.52

Preterm infants with low immunoglobulin G levels have increased risk of neonatal sepsis but do not benefit from prophylactic immunoglobulin G. J Pediatr (2000) 1.51

High tie versus low tie vascular ligation of the inferior mesenteric artery in colorectal cancer surgery: impact on the gain in colon length and implications on the feasibility of anastomoses. Dis Colon Rectum (2012) 1.51

Malignant degeneration in Madelung's disease (benign lipomatosis of the neck): case report. Br J Plast Surg (1983) 1.46

An unusual translocation of immunoglobulin gene segments in variants of the mouse myeloma MPC11. Nature (1980) 1.45

A case-control study of risk factors for intraoperative suprachoroidal expulsive hemorrhage. Ophthalmology (1991) 1.45

Hepatitis delta: on soft paws across Germany. Infection (2012) 1.43

Giant and reversible extrinsic magnetocaloric effects in La0.7Ca0.3MnO3 films due to strain. Nat Mater (2012) 1.43

Experience with split-course external beam irradiation +/- chemotherapy and integrated Ir-192 high-dose-rate brachytherapy in the treatment of primary carcinomas of the anal canal. Int J Radiat Oncol Biol Phys (2001) 1.41

Profiling left and right ventricular proportional output during fetal life with a novel systolic index in the aortic isthmus. Ultrasound Obstet Gynecol (2014) 1.39

On the size of the active site in proteases. II. Carboxypeptidase-A. Biochem Biophys Res Commun (1967) 1.39

Use of antibodies for the isolation of biologically pure messenger ribonucleic acid from fully functional eukaryotic cells. Biochemistry (1974) 1.39

How accurate is serologic testing of plasma pools for hepatitis B virus surface antigen, anti-human immunodeficiency virus 1 and 2, and anti-hepatitis C virus? Infusionsther Transfusionsmed (1996) 1.39

Rehabilitation after severe head injury. Scand J Rehabil Med (1974) 1.38

Determinants of sustainable CD4 lymphocyte count increases in response to antiretroviral therapy. AIDS (1999) 1.33

Taxol produces a predominantly sensory neuropathy. Neurology (1989) 1.32

Gram type-specific broad-range PCR amplification for rapid detection of 62 pathogenic bacteria. J Clin Microbiol (1999) 1.32

Antigenic competition between polypeptidyl determinants in normal and tolerant rabbits. J Exp Med (1968) 1.32

The fasciocutaneous supraclavicular artery island flap for releasing postburn mentosternal contractures. Plast Reconstr Surg (1997) 1.31

Amino acid-sequence variability at the N-terminal extra piece of mouse immunoglobulin light-chain precursors of the same and different subgroups. Biochem J (1976) 1.30

The localization of antigen in lymph nodes and its relation to specific antibody-producing cells. II. Comparison of iodine-125 and tritium labels. Immunology (1967) 1.29

A nuclear mutant of Arabidopsis with impaired stability on distinct transcripts of the plastid psbB, psbD/C, ndhH, and ndhC operons. Plant Cell (1996) 1.28

Involvement of a pertussis toxin-sensitive G protein in the mitogenic signaling pathways of sphingosine 1-phosphate. J Biol Chem (1995) 1.27

Depression and risk of heart failure among older persons with isolated systolic hypertension. Arch Intern Med (2001) 1.26

Messenger RNA of opsin from bovine retina: isolation and partial sequence of the in vitro translation product. Proc Natl Acad Sci U S A (1979) 1.25

Hepatitis B virus markers in anti-HBc only positive individuals. J Med Virol (2001) 1.23

Inhibition of return in spatial attention: direct evidence for collicular generation. Nat Neurosci (1999) 1.21

Identification of N-terminal methionine in the precursor of immunoglobulin light chain. Initiation of translation of messenger ribonucleic acid in plants and animals. Biochem J (1976) 1.20

Induction of the interleukin 1 receptor antagonist protein by transforming growth factor-beta. Eur J Immunol (1991) 1.20

Macromolecular mechanisms of sputum inhibition of tobramycin activity. Antimicrob Agents Chemother (1995) 1.19

Serum microRNA-122 levels in different groups of patients with chronic hepatitis B virus infection. J Viral Hepat (2011) 1.18

A treadmill ergometer for three-dimensional ground reaction forces measurement during walking. J Biomech (2001) 1.18

QTL detection by multi-parent linkage mapping in oil palm (Elaeis guineensis Jacq.). Theor Appl Genet (2010) 1.17

SNAP-23 is not cleaved by botulinum neurotoxin E and can replace SNAP-25 in the process of insulin secretion. J Biol Chem (1997) 1.17

Polymers tripeptides as collagen models. II. Conformational changes of poly(L-prolyl-glycyl-L-prolyl) in solution. J Mol Biol (1966) 1.16

Binding of 2,4-dinitrophenyl derivatives by the light chain dimer obtained from immunoglobulin A produced by MOPC-315 mouse myeloma. Biochemistry (1976) 1.14

A revolution in drug discovery. Combinatorial chemistry still needs logic to drive science forward. BMJ (2000) 1.14

Action on clinical audit: progress report. BMJ (1998) 1.12

Modulation of interleukin-18 expression in human colon carcinoma: consequences for tumor immune surveillance. Int J Cancer (1999) 1.11

Partial sequence of the precursors of immunoglobulin light-chains of different subgroups: evidence that the immunoglobulin variable-region gene is larger than hitherto known. Biochem Biophys Res Commun (1976) 1.11

Retinal pigment epithelial repopulation in monkeys after submacular surgery. Arch Ophthalmol (1995) 1.11

Lysine oligopeptides. Preparation by ion-exchange chromatography. Biopolymers (1972) 1.11

Phantoms in the brain. Question the assumption that the adult brain is "hard wired". BMJ (1999) 1.11

A dipeptidocarboxypeptidase from E. coli. Biochem Biophys Res Commun (1972) 1.11

Multicenter evaluation of a new rapid automated human immunodeficiency virus antigen detection assay. J Virol Methods (1999) 1.11

Attitudes of high school girls in Israel and the United States toward breast feeding. J Adolesc Health Care (1985) 1.10

Divergence in signal transduction pathways of platelet-derived growth factor (PDGF) and epidermal growth factor (EGF) receptors. Involvement of sphingosine 1-phosphate in PDGF but not EGF signaling. J Biol Chem (1997) 1.10

Synthetic polypeptides as substrates and inhibitors of collagen proline hydroxylase. Arch Biochem Biophys (1968) 1.10

Combining sites of antibodies with L-alanine and D-alanine peptide specificity and the effect of serum proteolytic activity on their estimation. Biochim Biophys Acta (1966) 1.09

Th1 and Th2 responses: what are they? BMJ (2000) 1.09

A comprehensive overview of grain development in Brachypodium distachyon variety Bd21. J Exp Bot (2011) 1.08

The synthesis and circular dichroism of a series of peptides possessing the structure (L-tyrosyl-L-alanyl-L-glutamyl)n. Eur J Biochem (1971) 1.08

Percutaneous endoscopic gastrostomy (PEG). 8 years of clinical experience in 232 patients. Surg Endosc (1997) 1.08

Synthesis and physicochemical properties in aqueous solution of the sequential polypeptide poly(Tyr-Ala-Glu). Biopolymers (1971) 1.07

Human fibulin-4: analysis of its biosynthetic processing and mRNA expression in normal and tumour tissues. FEBS Lett (2001) 1.07

The renaissance of C reactive protein. BMJ (2001) 1.07

Glutamine as a precursor to N-terminal pyrrolid-2-one-5-carboxylic acid in mouse immunoglobulin lambda-type light chains. Amino acid-sequence variability at the N-terminal extra piece of lambda-type light-chain precursors. Biochem J (1977) 1.07

Rapid diagnosis of enterovirus infection by a new one-step reverse transcription-PCR assay. J Clin Microbiol (1997) 1.06

Interleukin-18: biological properties and clinical implications. Eur Cytokine Netw (2000) 1.06

Cystic thymoma simulating contrictive pericarditis. The role of echocardiography in the differential diagnosis. J Thorac Cardiovasc Surg (1975) 1.06

Identification and fine mapping of Pi33, the rice resistance gene corresponding to the Magnaporthe grisea avirulence gene ACE1. Theor Appl Genet (2003) 1.06

Further experience with interfascicular grafting of the median, ulnar, and radial nerves. J Bone Joint Surg Am (1976) 1.04

Somatic diversification of chicken immunoglobulin light chains by point mutations. Proc Natl Acad Sci U S A (1990) 1.04

Cellular mechanisms of rejection and regeneration in peripheral nerve allografts. Transplantation (1989) 1.03

Local anesthetic inhibition of G protein-coupled receptor signaling by interference with Galpha(q) protein function. Mol Pharmacol (2001) 1.03

Evaluation of factors controlling glucose tolerance in patients with HCV infection before and after 4 months therapy with interferon-alpha. Eur J Clin Invest (2000) 1.03

Recovery of communicative functions after prolonged traumatic coma. Scand J Rehabil Med (1978) 1.03