One-step purification of hybrid proteins which have beta-galactosidase activity.

PubWeight™: 2.77‹?› | Rank: Top 1%

🔗 View Article (PMID 6436145)

Published in Gene on December 13, 1984

Authors

A Ullmann

Articles citing this

Shuttle mutagenesis: a method of transposon mutagenesis for Saccharomyces cerevisiae. Proc Natl Acad Sci U S A (1986) 5.75

Extent of N-terminal methionine excision from Escherichia coli proteins is governed by the side-chain length of the penultimate amino acid. Proc Natl Acad Sci U S A (1989) 4.94

Strategies for achieving high-level expression of genes in Escherichia coli. Microbiol Rev (1996) 4.93

RecA protein-dependent cleavage of UmuD protein and SOS mutagenesis. Proc Natl Acad Sci U S A (1988) 4.12

lac repressor forms loops with linear DNA carrying two suitably spaced lac operators. EMBO J (1987) 4.02

The nuclear migration signal of Xenopus laevis nucleoplasmin. EMBO J (1987) 3.36

The primary structure of human dopamine-beta-hydroxylase: insights into the relationship between the soluble and the membrane-bound forms of the enzyme. EMBO J (1987) 3.06

Immobilization and purification of enzymes with staphylococcal protein A gene fusion vectors. EMBO J (1985) 2.91

Nuclear transport kinetics depend on phosphorylation-site-containing sequences flanking the karyophilic signal of the Simian virus 40 T-antigen. EMBO J (1989) 2.40

The regulatory VirG protein specifically binds to a cis-acting regulatory sequence involved in transcriptional activation of Agrobacterium tumefaciens virulence genes. J Bacteriol (1990) 2.24

nit-2, the major positive-acting nitrogen regulatory gene of Neurospora crassa, encodes a sequence-specific DNA-binding protein. Proc Natl Acad Sci U S A (1990) 2.24

Escherichia coli-mycobacteria shuttle vectors for operon and gene fusions to lacZ: the pJEM series. J Bacteriol (1994) 2.13

The Aspergillus nidulans abaA gene encodes a transcriptional activator that acts as a genetic switch to control development. Mol Cell Biol (1994) 1.94

Nucleotide sequence of the argR gene of Escherichia coli K-12 and isolation of its product, the arginine repressor. Proc Natl Acad Sci U S A (1987) 1.67

Cross talk to the phosphate regulon of Escherichia coli by PhoM protein: PhoM is a histidine protein kinase and catalyzes phosphorylation of PhoB and PhoM-open reading frame 2. J Bacteriol (1990) 1.60

Selective expression of a protein-tyrosine kinase, p56lyn, in hematopoietic cells and association with production of human T-cell lymphotropic virus type I. Proc Natl Acad Sci U S A (1989) 1.59

Molecular analysis of the Alcaligenes eutrophus poly(3-hydroxybutyrate) biosynthetic operon: identification of the N terminus of poly(3-hydroxybutyrate) synthase and identification of the promoter. J Bacteriol (1991) 1.54

Identification of a human protein that interacts with nuclear localization signals. J Cell Biol (1989) 1.52

GCR1 of Saccharomyces cerevisiae encodes a DNA binding protein whose binding is abolished by mutations in the CTTCC sequence motif. Proc Natl Acad Sci U S A (1991) 1.45

The L2 open reading frame of human papillomavirus type 1a encodes a minor structural protein carrying type-specific antigens. J Virol (1986) 1.41

Localization of the Antennapedia protein in Drosophila embryos and imaginal discs. EMBO J (1986) 1.35

cDNA clones of the auxin-binding protein from corn coleoptiles (Zea mays L.): isolation and characterization by immunological methods. EMBO J (1989) 1.33

Identification and characterization of two Alcaligenes eutrophus gene loci relevant to the poly(beta-hydroxybutyric acid)-leaky phenotype which exhibit homology to ptsH and ptsI of Escherichia coli. J Bacteriol (1991) 1.23

Expression of active hormone and DNA-binding domains of the chicken progesterone receptor in E. coli. EMBO J (1989) 1.19

The chromosomally encoded cation diffusion facilitator proteins DmeF and FieF from Wautersia metallidurans CH34 are transporters of broad metal specificity. J Bacteriol (2004) 1.17

Development of type-specific and cross-reactive serological probes for the minor capsid protein of human papillomavirus type 33. J Virol (1993) 1.11

The 11-1 gene of Plasmodium falciparum codes for distinct fast evolving repeats. EMBO J (1988) 1.08

RNA-binding activity of the matK protein encoded by the chloroplast trnK intron from mustard (Sinapis alba L.). Nucleic Acids Res (1995) 1.07

Defining a minimal estrogen receptor DNA binding domain. Nucleic Acids Res (1993) 1.06

Differential antero-posterior expression of two proteins encoded by a homeobox gene in Xenopus and mouse embryos. EMBO J (1988) 1.06

Enhanced phosphorylation of the C-terminal domain of RNA polymerase II upon serum stimulation of quiescent cells: possible involvement of MAP kinases. EMBO J (1994) 1.05

Lack of cleavage of IcsA in Shigella flexneri causes aberrant movement and allows demonstration of a cross-reactive eukaryotic protein. Infect Immun (1996) 1.00

Production of specific antibodies against protein A fusion proteins. EMBO J (1986) 0.97

Identification and characterization of the products from the traJ and traY genes of plasmid R100. J Bacteriol (1988) 0.97

Rhizobium meliloti adenylate cyclase is related to eucaryotic adenylate and guanylate cyclases. J Bacteriol (1990) 0.97

Enzyme-linked immunosorbent assay for Escherichia coli heat-stable enterotoxin type II. J Clin Microbiol (1988) 0.93

Characterization of Lactococcus lactis phage antigens. Appl Environ Microbiol (1992) 0.91

Expression of the cloned gene for enterotoxin STb of Escherichia coli. Infect Immun (1990) 0.90

Human DNA polymerase alpha catalytic polypeptide binds ConA and RCA and contains a specific labile site in the N-terminus. Nucleic Acids Res (1990) 0.87

Anti-Cdc25 antibodies inhibit guanyl nucleotide-dependent adenylyl cyclase of Saccharomyces cerevisiae and cross-react with a 150-kilodalton mammalian protein. Mol Cell Biol (1992) 0.87

Cellular responses to a 55-kilodalton recombinant Pneumocystis carinii antigen. Infect Immun (1994) 0.86

Localization of myosin heavy chain A in the human pathogen Entamoeba histolytica. Infect Immun (1993) 0.85

Recombinant hydrophilic region of murine retroviral protein p15E inhibits stimulated T-lymphocyte proliferation. Proc Natl Acad Sci U S A (1987) 0.85

Expression in Escherichia coli of a cloned DNA sequence encoding the pre-S2 region of hepatitis B virus. Proc Natl Acad Sci U S A (1985) 0.84

Identification and isolation of the polyferredoxin from Methanobacterium thermoautotrophicum strain delta H. Proc Natl Acad Sci U S A (1992) 0.84

Identification of the mcrD gene product and its association with component C of methyl coenzyme M reductase in Methanococcus vannielii. J Bacteriol (1990) 0.77

Functional analysis of mouse Hoxa-7 in Saccharomyces cerevisiae: sequences outside the homeodomain base contact zone influence binding and activation. Mol Cell Biol (1994) 0.76

Integrated R2 sequence in mitochondria of fertile B37N maize encodes and expresses a 130 kD polypeptide similar to that encoded by the S2 episome of S-type male sterile plants. Nucleic Acids Res (1989) 0.75

Expression of the extracellular domain of the human immunodeficiency virus type 1 envelope protein and its fusion with beta-galactosidase in Saccharomyces cerevisiae. Clin Diagn Lab Immunol (1998) 0.75

NanoLuc Luciferase - A Multifunctional Tool for High Throughput Antibody Screening. Front Pharmacol (2016) 0.75

Articles by these authors

A bacterial two-hybrid system based on a reconstituted signal transduction pathway. Proc Natl Acad Sci U S A (1998) 9.05

Secretion of cyclolysin, the calmodulin-sensitive adenylate cyclase-haemolysin bifunctional protein of Bordetella pertussis. EMBO J (1988) 4.75

The calmodulin-sensitive adenylate cyclase of Bordetella pertussis: cloning and expression in Escherichia coli. Mol Microbiol (1988) 4.15

Characterization by in vitro complementation of a peptide corresponding to an operator-proximal segment of the beta-galactosidase structural gene of Escherichia coli. J Mol Biol (1967) 3.43

On the subunit structure of wild-type versus complemented beta-galactosidase of Escherichia coli. J Mol Biol (1968) 2.44

Bordatella pertussis adenylate cyclase: a toxin with multiple talents. Trends Microbiol (1999) 2.22

[Identification, by in vitro complementation and purification, of a peptide fraction of Escherichia coli beta-galactosidase]. J Mol Biol (1965) 2.18

Cyclic AMP as a modulator of polarity in polycistronic transcriptional units. Proc Natl Acad Sci U S A (1979) 1.88

Cloning and expression of the calmodulin-sensitive Bacillus anthracis adenylate cyclase in Escherichia coli. Gene (1988) 1.86

Virulence dependent and independent regulation of the Bordetella pertussis cya operon. EMBO J (1990) 1.75

Protein-protein interaction between Bacillus stearothermophilus tyrosyl-tRNA synthetase subdomains revealed by a bacterial two-hybrid system. J Mol Microbiol Biotechnol (2001) 1.67

A bacterial two-hybrid system that exploits a cAMP signaling cascade in Escherichia coli. Methods Enzymol (2000) 1.63

Expression and regulation of lactose genes carried by plasmids. J Bacteriol (1976) 1.53

Catabolite repression in Escherichia coli mutants lacking cyclic AMP. Mol Gen Genet (1978) 1.50

Response to imatinib in patients who relapse after allogeneic stem cell transplantation for chronic myeloid leukemia. Leukemia (2003) 1.47

Calmodulin-activated bacterial adenylate cyclases as virulence factors. Trends Microbiol (1993) 1.44

Adenylate cyclase toxin (CyaA) of Bordetella pertussis. Evidence for the formation of small ion-permeable channels and comparison with HlyA of Escherichia coli. J Biol Chem (1994) 1.41

Deletions affecting hemolytic and toxin activities of Bordetella pertussis adenylate cyclase. Infect Immun (1990) 1.41

Induction of apoptosis in human papillomaviruspositive cancer cells by peptide aptamers targeting the viral E6 oncoprotein. Proc Natl Acad Sci U S A (2000) 1.38

Multiple regulation of the activity of adenylate cyclase in Escherichia coli. Mol Gen Genet (1982) 1.37

Insertional mutagenesis of Bordetella pertussis adenylate cyclase. J Biol Chem (1992) 1.36

In vivo induction of CTL responses by recombinant adenylate cyclase of Bordetella pertussis carrying viral CD8+ T cell epitopes. J Immunol (1996) 1.33

Are cyclic AMP effects related to real physiological phenomena? Biochem Biophys Res Commun (1974) 1.32

Functional p53 protein in human papillomavirus-positive cancer cells. Oncogene (1995) 1.31

Phosphorylated BvgA is sufficient for transcriptional activation of virulence-regulated genes in Bordetella pertussis. EMBO J (1996) 1.29

Catabolite modulator factor: a possible mediator of catabolite repression in bacteria. Proc Natl Acad Sci U S A (1976) 1.28

Anti-viral protection conferred by recombinant adenylate cyclase toxins from Bordetella pertussis carrying a CD8+ T cell epitope from lymphocytic choriomeningitis virus. Proc Natl Acad Sci U S A (1997) 1.28

Phosphorylation-dependent binding of BvgA to the upstream region of the cyaA gene of Bordetella pertussis. Mol Microbiol (1996) 1.27

Catabolite repression in Escherichia coli mutants lacking cyclic AMP receptor protein. Proc Natl Acad Sci U S A (1980) 1.27

Identification of the Escherichia coli cya gene product as authentic adenylate cyclase. J Mol Biol (1984) 1.25

Pleiotropic effects of cAMP on germination, antibiotic biosynthesis and morphological development in Streptomyces coelicolor. Mol Microbiol (1998) 1.25

On the determination of molecular weight of proteins and protein subunits in the presence of 6 M guanidine hydrochloride. Biochemistry (1968) 1.21

Formylation of initiator tRNA methionine in procaryotic protein synthesis: in vivo polarity in lactose operon expression. J Bacteriol (1978) 1.20

Charge-dependent translocation of Bordetella pertussis adenylate cyclase toxin into eukaryotic cells: implication for the in vivo delivery of CD8(+) T cell epitopes into antigen-presenting cells. Proc Natl Acad Sci U S A (1998) 1.19

Cell-invasive activity of epitope-tagged adenylate cyclase of Bordetella pertussis allows in vitro presentation of a foreign epitope to CD8+ cytotoxic T cells. Infect Immun (1995) 1.17

High-level synthesis of active adenylate cyclase toxin of Bordetella pertussis in a reconstructed Escherichia coli system. Gene (1991) 1.16

Therapy of murine tumors with recombinant Bordetella pertussis adenylate cyclase carrying a cytotoxic T cell epitope. J Immunol (1999) 1.13

Two functional domains in adenylate cyclase of Escherichia coli. J Mol Biol (1983) 1.11

Direct delivery of the Bordetella pertussis adenylate cyclase toxin to the MHC class I antigen presentation pathway. J Immunol (1999) 1.11

Hemolytic, but not cell-invasive activity, of adenylate cyclase toxin is selectively affected by differential fatty-acylation in Escherichia coli. J Biol Chem (1995) 1.10

A novel chromatin-forming histone H1 homologue is encoded by a dispensable and growth-regulated gene in Bordetella pertussis. Mol Microbiol (1995) 1.09

An immunological study of complementary fragments of beta-galactosidase. Biochemistry (1974) 1.09

Vasculitis-like syndrome associated with Borrelia lusitaniae infection. Clin Rheumatol (2008) 1.08

Sensitive genetic screen for protease activity based on a cyclic AMP signaling cascade in Escherichia coli. J Bacteriol (2000) 1.07

Analysis of Bordetella pertussis cya operon regulation by use of cya-lac fusions. FEMS Microbiol Lett (1991) 1.07

Isolation and characterization of an Escherichia coli mutant affected in the regulation of adenylate cyclase. J Bacteriol (1981) 1.06

Cellular responses of HPV-positive cancer cells to genotoxic anti-cancer agents: repression of E6/E7-oncogene expression and induction of apoptosis. Int J Cancer (1996) 1.05

Properties of cyclic AMP-independent catabolite gene activator proteins of Escherichia coli. J Biol Chem (1986) 1.01

Identification by in vitro complementation of regions required for cell-invasive activity of Bordetella pertussis adenylate cyclase toxin. Mol Microbiol (1995) 0.99

Immunological relatedness between Bordetella pertussis and rat brain adenylyl cyclases. Biochemistry (1988) 0.97

Regulation of galactose operon expression: glucose effects and role of cyclic adenosine 3',5'-monophosphate. J Bacteriol (1981) 0.97

Induction of the p53-target gene GADD45 in HPV-positive cancer cells. Oncogene (1999) 0.96

[Catabolic repression, 1970]. Biochimie (1971) 0.94

Characterization of DNA binding sites for the BvgA protein of Bordetella pertussis. J Bacteriol (1997) 0.93

Transcriptional control of polarity in Escherichia coli by cAMP. Mol Gen Genet (1984) 0.93

Catabolite repression 1985. Biochimie (1985) 0.93

Quantitative ultramicro-scale immunoenzymic method for measuring Ig antigenic determinants in single cells. Clin Chem (1978) 0.89

On the effect of divalent cations and protein concentration upon renaturation of beta-galactosidase from E. coli. Biochem Biophys Res Commun (1969) 0.88

Hybrid Bordetella pertussis-Escherichia coli RNA polymerases: selectivity of promoter activation. J Bacteriol (1998) 0.87

Catabolite modulator factor: physiological properties and in vivo effects. Mol Gen Genet (1978) 0.87

Peptide aptamers targeting the hepatitis B virus core protein: a new class of molecules with antiviral activity. Oncogene (2001) 0.87

Functional analysis of the cya promoter of Bordetella pertussis. Mol Microbiol (1993) 0.87

Mutations in the Bordetella pertussis bvgS gene that confer altered expression of the fhaB gene in Escherichia coli. J Bacteriol (1994) 0.86

Bordetella pertussis adenylate cyclase: the gene and the protein. Tokai J Exp Clin Med (1988) 0.84

The Bordetella pertussis sigma subunit of RNA polymerase confers enhanced expression of fha in Escherichia coli. Mol Microbiol (1997) 0.84

The adenylate cyclase catalytic domain of Streptomyces coelicolor is carboxy-terminal. FEMS Microbiol Lett (1993) 0.83

Bordetella pertussis adenylate cyclase toxin: a vehicle to deliver CD8-positive T-cell epitopes into antigen-presenting cells. Methods Enzymol (2000) 0.83

Uncoupling of p21WAF1/CIP1/SDI1 mRNA and protein expression upon genotoxic stress. Oncogene (1998) 0.83

The effect of urea on catabolite sensitive operons in Escherichia coli K 12. Mol Gen Genet (1980) 0.82

Identification of a common domain in calmodulin-activated eukaryotic and bacterial adenylate cyclases. Biochemistry (1989) 0.80

[Molecular mass and extinction coefficient of the glycogen phosphorylase b of rabbit muscle]. Biochimie (1971) 0.79

Bordetella pertussis adenylate cyclase toxin as a tool to analyze molecular interactions in a bacterial two-hybrid system. Int J Med Microbiol (2000) 0.79

Mutations which result in constitutive expression of the Bordetella pertussis filamentous haemagglutinin gene. Res Microbiol (1995) 0.79

Transcription-translation coupling and polarity: a possible role of cyclic AMP. Biochimie (1981) 0.78

Differential cell cycle response of nontumorigenic and tumorigenic human papillomavirus-positive keratinocytes towards transforming growth factor-beta1. J Mol Med (Berl) (2000) 0.78

Affinity-based chromatography utilizing genetically engineered proteins. Interaction of Bordetella pertussis adenylate cyclase with calmodulin. J Biol Chem (1988) 0.77

An Escherichia coli insertion element (IS2) provides a functional promoter in Bordetella pertussis. Res Microbiol (1992) 0.77

Two different mechanisms for urea action at the LAC and TNA operons in Escherichia coli. Mol Gen Genet (1990) 0.77

Modulation of the lactose operon mRNA turnover by inhibitors of dihydrofolate reductase. Biochem Biophys Res Commun (1978) 0.77

Urea, a specific inhibitor of catabolite sensitive operons. Biochem Biophys Res Commun (1976) 0.76

Bordetella pertussis adenylate cyclase: a toxin with multiple talents. Zentralbl Bakteriol (1993) 0.76

Regulation of cyclic AMP synthesis in Escherichia coli K-12: effects of the rpoD800 sigma mutation, glucose, and chloramphenicol. J Bacteriol (1984) 0.75

André Lwoff (1902-1994): remembrances. EMBO J (1995) 0.75

Cloning of a coding sequence of "Bordetella pertussis" filamentous hemagglutinin gene. Ann Sclavo Collana Monogr (1986) 0.75

A social work perspective on ethical practice in end-stage renal disease. Soc Work Health Care (1984) 0.75

Regulation of plasmid-encoded lactose genes in different gram-negative enterobacteriaceae. Plasmid (1980) 0.75

An immunological determination of specific activities of enzymes: its use in quantification of cross reactivity between enzymes of different origins. Biochimie (1978) 0.75

Chemical fatty acylation confers hemolytic and toxic activities to adenylate cyclase protoxin of Bordetella pertussis. J Biol Chem (1994) 0.75

[Hydrolysis of urinary metabolites of different steroid hormones by -glucuronidase from Escherichia coli]. Ann Inst Pasteur (Paris) (1972) 0.75

Immunological relatedness between beta-galactosidases of different bacterial origins. Mol Immunol (1979) 0.75

Social structure and social behavior in an intensive care unit: patient-family perspectives. A social worker's response. Soc Work Health Care (1980) 0.75

Pleiotropic expression of catabolic operons in the absence of the cAMP-CAP complex: the case of the maltose regulon. Ann Microbiol (Paris) (1982) 0.75

[Hydrolysis of glucosiduronates of urinary steroids related to pregnane and estrane by a new fast-acting E. coli beta-glucuronidase]. C R Acad Sci Hebd Seances Acad Sci D (1973) 0.75

A gerontology internship program for medical students. Soc Work Health Care (1985) 0.75

Health care in a selected urban elderly population. Utilization patterns and perceived needs. Gerontologist (1977) 0.75

Volunteers boost spirits of elderly. Hospitals (1980) 0.75

[Daniel Bovet and the Pasteur Institute]. Ann Ist Super Sanita (1993) 0.75

Relevance of depression for anticoagulation management in a routine medical care setting: results from the ThrombEVAL study program. J Thromb Haemost (2014) 0.75