Helix packing in the sucrose permease of Escherichia coli: properties of engineered charge pairs between helices VII and XI.

PubWeight™: 0.93‹?›

🔗 View Article (PMID 7626606)

Published in Biochemistry on July 25, 1995

Authors

S Frillingos1, M Sahin-Tóth, J W Lengeler, H R Kaback

Author Affiliations

1: Howard Hughes Medical Institute, Department of Physiology, University of California, Los Angeles 90095-1662, USA.

Articles by these authors

Phosphoenolpyruvate:carbohydrate phosphotransferase systems of bacteria. Microbiol Rev (1993) 11.25

Phosphoenolpyruvate:carbohydrate phosphotransferase system of bacteria. Microbiol Rev (1985) 5.90

Cloning and functional expression in bacteria of a novel glucose transporter present in liver, intestine, kidney, and beta-pancreatic islet cells. Cell (1988) 5.18

The role of the phosphoenolpyruvate-phosphotransferase system in the transport of sugars by isolated membrane preparations of Escherichia coli. J Biol Chem (1968) 4.60

Transport across isolated bacterial cytoplasmic membranes. Biochim Biophys Acta (1972) 4.43

Proline uptake by an isolated cytoplasmic membrane preparation of Escherichia coli. Proc Natl Acad Sci U S A (1966) 4.37

Transport. Annu Rev Biochem (1970) 4.09

The electrochemical gradient of protons and its relationship to active transport in Escherichia coli membrane vesicles. Proc Natl Acad Sci U S A (1976) 3.91

Mechanisms of active transport in isolated bacterial membrane vesicles. 8. The transport of amino acids by membranes prepared from Escherichia coli. J Biol Chem (1972) 3.65

Cys-scanning mutagenesis: a novel approach to structure function relationships in polytopic membrane proteins. FASEB J (1998) 3.41

Membrane potential and active transport in membrane vesicles from Escherichia coli. Biochemistry (1975) 3.36

Mechanisms of active transport in isolated membrane vesicles. II. The mechanism of energy coupling between D-lactic dehydrogenase and beta-galactoside transport in membrane preparations from Escherichia coli. J Biol Chem (1971) 3.24

The kamikaze approach to membrane transport. Nat Rev Mol Cell Biol (2001) 3.09

Mechanisms of active transport in isolated membrane vesicles. I. The site of energy coupling between D-lactic dehydrogenase and beta-galactoside transport in Escherichia coli membrane vesicles. J Biol Chem (1971) 2.94

Purification and reconstitution of functional lactose carrier from Escherichia coli. J Biol Chem (1981) 2.93

Structure of the lac carrier protein of Escherichia coli. J Biol Chem (1983) 2.92

Mechanisms of active transport in isolated membrane vesicles. 2. The coupling of reduced phenazine methosulfate to the concentrative uptake of beta-galactosides and amino acids. J Biol Chem (1971) 2.78

Transport in isolated bacterial membrane vesicles. Methods Enzymol (1974) 2.75

Membrane potential and gentamicin uptake in Staphylococcus aureus. Proc Natl Acad Sci U S A (1982) 2.73

The relationship between the electrochemical proton gradient and active transport in Escherichia coli membrane vesicles. Biochemistry (1977) 2.70

Quantitative measurements of membrane potential in Escherichia coli. Biochemistry (1980) 2.66

Localization of D-lactate dehydrogenase in native and reconstituted Escherichia coli membrane vesicles. J Biol Chem (1975) 2.64

Relationship of a membrane-bound D-(-)-lactic dehydrogenase to amino acid transport in isolated bacterial membrane preparations. Proc Natl Acad Sci U S A (1970) 2.63

Mechanisms of active transport in isolated bacterial membrane vesicles. 18. The mechanism of action of carbonylcyanide m-chlorophenylhydrazone. Arch Biochem Biophys (1974) 2.61

Transport studies in bacterial membrane vesicles. Science (1974) 2.54

Purification, reconstitution, and characterization of the lac permease of Escherichia coli. Methods Enzymol (1986) 2.32

The lac carrier protein in Escherichia coli. J Membr Biol (1983) 2.29

Proline uptake by disrupted membrane preparations from Escherichia coli. Arch Biochem Biophys (1969) 2.29

Mutants of Salmonella typhimurium and Escherichia coli pleiotropically defective in active transport. Proc Natl Acad Sci U S A (1972) 2.22

Beta-galactoside transport in bacterial membrane preparations: energy coupling via membrane-bounded D-lactic dehydrogenase. Proc Natl Acad Sci U S A (1970) 2.22

The electrochemical proton gradient in Escherichia coli membrane vesicles. Biochemistry (1977) 2.08

Mechanism of lactose translocation in membrane vesicles from Escherichia coli. 1. Effect of pH on efflux, exchange, and counterflow. Biochemistry (1979) 2.07

Use of a lipophilic cation for determination of membrane potential in neuroblastoma-glioma hybrid cell suspensions. Proc Natl Acad Sci U S A (1979) 2.06

NADH-ubiquinone oxidoreductases of the Escherichia coli aerobic respiratory chain. Biochemistry (1987) 2.04

The use of flow dialysis for determinations of deltapH and active transport. Methods Enzymol (1979) 1.94

Membrane potential changes during mitogenic stimulation of mouse spleen lymphocytes. Proc Natl Acad Sci U S A (1980) 1.90

A phospholipid acts as a chaperone in assembly of a membrane transport protein. J Biol Chem (1996) 1.89

Anaerobic transport in Escherichia coli membrane vesicles. Proc Natl Acad Sci U S A (1973) 1.88

Molecular biology and energetics of membrane transport. J Cell Physiol (1976) 1.86

Mechanism of lactose translocation in membrane vesicles from Escherichia coli. 2. Effect of imposed delata psi, delta pH, and Delta mu H+. Biochemistry (1979) 1.85

Quantitative association between electrical potential across the cytoplasmic membrane and early gentamicin uptake and killing in Staphylococcus aureus. J Bacteriol (1984) 1.84

Minigene analysis of intronic variants in common SPINK1 haplotypes associated with chronic pancreatitis. Gut (2008) 1.83

Regulation of sugar transport in isolated bacterial membrane preparations from Escherichia coli. Proc Natl Acad Sci U S A (1969) 1.82

Climate change and the integrity of science. Science (2010) 1.80

Electrochemical proton gradient in inverted membrane vesicles from Escherichia coli. Biochemistry (1980) 1.78

Cysteine scanning mutagenesis of putative transmembrane helices IX and X in the lactose permease of Escherichia coli. Protein Sci (1993) 1.78

Construction of a functional lactose permease devoid of cysteine residues. Biochemistry (1991) 1.77

Glycine uptake in Escherichia coli. II. Glycine uptake, exchange, and metabolism by an isolated membrane preparation. J Biol Chem (1968) 1.76

Sodium-dependent methyl 1-thio-beta-D-galactopyranoside transport in membrane vesicles isolated from Salmonella typhimurium. Biochemistry (1977) 1.75

Preparation, characterization, and properties of monoclonal antibodies against the lac carrier protein from Escherichia coli. Proc Natl Acad Sci U S A (1982) 1.74

Mechanisms of active transport in isolated membrane vesicles. IV. Galactose transport by isolated membrane vesicles from Escherichia coli. J Biol Chem (1972) 1.74

Mechanisms of active transport in isolated bacterial membrane vesicles. Further studies on amino acid transport in Staphylococcus aureus membrane vesicles. J Biol Chem (1974) 1.73

Active transport in Escherichia coli: passage to permease. Annu Rev Biophys Biophys Chem (1986) 1.72

In vivo expression of the lacY gene in two segments leads to functional lac permease. Proc Natl Acad Sci U S A (1990) 1.71

CTnscr94, a conjugative transposon found in enterobacteria. J Bacteriol (1997) 1.70

Macrophage membrane potential changes associated with gamma 2b/gamma 1 Fc receptor-ligand binding. Proc Natl Acad Sci U S A (1983) 1.70

Properties of permease dimer, a fusion protein containing two lactose permease molecules from Escherichia coli. Proc Natl Acad Sci U S A (1994) 1.68

Intramolecular dislocation of the COOH terminus of the lac carrier protein in reconstituted proteoliposomes. Proc Natl Acad Sci U S A (1984) 1.68

Molecular biology of active transport: from membrane to molecule to mechanism. Harvey Lect (1989) 1.65

Mouse macrophage Fc receptor for IgG gamma 2b/gamma 1 in artificial and plasma membrane vesicles functions as a ligand-dependent ionophore. Proc Natl Acad Sci U S A (1983) 1.64

Fourier transform infrared spectroscopy reveals a rigid alpha-helical assembly for the tetrameric Streptomyces lividans K+ channel. Proc Natl Acad Sci U S A (1998) 1.62

Membrane potential in anaerobically growing Staphylococcus aureus and its relationship to gentamicin uptake. Antimicrob Agents Chemother (1983) 1.62

Reconstitution of active transport in proteoliposomes containing cytochrome o oxidase and lac carrier protein purified from Escherichia coli. Proc Natl Acad Sci U S A (1983) 1.60

Active transport in membrane vesicles from Escherichia coli: the electrochemical proton gradient alters the distribution of the lac carrier between two different kinetic states. Biochemistry (1980) 1.60

Molecular structure of membrane vesicles from Escherichia coli. Proc Natl Acad Sci U S A (1978) 1.60

Mechanisms of active transport in isolated bacterial membrane vesicles. XV. Purification and properties of the membrane-bound D-lactate dehydrogenase from Escherichia coli. J Biol Chem (1973) 1.60

Molecular analysis of the gat genes from Escherichia coli and of their roles in galactitol transport and metabolism. J Bacteriol (1996) 1.55

Use of site-directed fluorescence labeling to study proximity relationships in the lactose permease of Escherichia coli. Biochemistry (1993) 1.54

Monoclonal antibodies against the lac carrier protein from Escherichia coli. 2. Binding studies with membrane vesicles and proteoliposomes reconstituted with purified lac carrier protein. Biochemistry (1984) 1.54

Equilibrium between two forms of the lac carrier protein in energized and nonenergized membrane vesicles from Escherichia coli. Biochemistry (1976) 1.54

Glycine uptake in Escherichia coli. I. Glycine uptake by whole cells of Escherichia coli W+ and a D-serine-resistant. J Biol Chem (1968) 1.54

Coupling the phosphotransferase system and the methyl-accepting chemotaxis protein-dependent chemotaxis signaling pathways of Escherichia coli. Proc Natl Acad Sci U S A (1995) 1.53

Energy-dependent binding of dansylgalactosides to the beta-galactoside carrier protein. J Biol Chem (1975) 1.49

Topology of the lac carrier protein in the membrane of Escherichia coli. Proc Natl Acad Sci U S A (1983) 1.48

cys154 Is important for lac permease activity in Escherichia coli. Biochem Biophys Res Commun (1985) 1.48

Characterization of Glu126 and Arg144, two residues that are indispensable for substrate binding in the lactose permease of Escherichia coli. Biochemistry (1999) 1.47

pH-dependent changes in proton:substrate stoichiometries during active transport in Escherichia coli membrane vesicles. Biochemistry (1977) 1.47

Active transport in isolated bacterial membrane vesicles. V. The transport of amino acids by membrane vesicles prepared from Staphylococcus aureus. J Biol Chem (1972) 1.47

The role of phosphatidylglycerol in the vectorial phosphorylation of sugar by isolated bacterial membrane preparations. Proc Natl Acad Sci U S A (1970) 1.46

lac permease of Escherichia coli: histidine-322 and glutamic acid-325 may be components of a charge-relay system. Biochemistry (1986) 1.46

Cysteine 148 in the lactose permease of Escherichia coli is a component of a substrate binding site. 2. Site-directed fluorescence studies. Biochemistry (1994) 1.44

Role of the charge pair aspartic acid-237-lysine-358 in the lactose permease of Escherichia coli. Biochemistry (1993) 1.44

Plasmid-mediated sucrose metabolism in Escherichia coli K12: mapping of the scr genes of pUR400. Mol Microbiol (1988) 1.44

Extracellular ATP perturbs transmembrane ion fluxes, elevates cytosolic [Ca2+], and inhibits phagocytosis in mouse macrophages. J Biol Chem (1985) 1.43

Properties and purification of an active biotinylated lactose permease from Escherichia coli. Proc Natl Acad Sci U S A (1993) 1.43

Characterization of site-directed mutants in the lac permease of Escherichia coli. 2. Glutamate-325 replacements. Biochemistry (1989) 1.42

The substrate-binding site in the lactose permease of Escherichia coli. Proc Natl Acad Sci U S A (1998) 1.41

Antigenic architecture of membrane vesicles from Escherichia coli. Biochemistry (1979) 1.40

Purified reconstituted lac carrier protein from Escherichia coli is fully functional. Proc Natl Acad Sci U S A (1984) 1.40

Probing the conformation of the lactose permease of Escherichia coli by in situ site-directed sulfhydryl modification. Biochemistry (1996) 1.39

Mechanisms of active transport in isolated bacterial membrane vesicles. XII. Active transport by a mutant of Escherichia coli uncoupled for oxidative phosphorylation. Arch Biochem Biophys (1973) 1.39

Identification of the epitope for monoclonal antibody 4B1 which uncouples lactose and proton translocation in the lactose permease of Escherichia coli. Biochemistry (1996) 1.38

Functional interactions between putative intramembrane charged residues in the lactose permease of Escherichia coli. Proc Natl Acad Sci U S A (1992) 1.37

Mechanism of lactose translocation in proteoliposomes reconstituted with lac carrier protein purified from Escherichia coli. 1. Effect of pH and imposed membrane potential on efflux, exchange, and counterflow. Biochemistry (1983) 1.37

Pathways for the utilization of N-acetyl-galactosamine and galactosamine in Escherichia coli. Mol Microbiol (2000) 1.37

Mechanisms of active transport in isolated bacterial membrane vesicles. IX. The kinetics and specificity of amino acid transport in Staphylococcus aureus membrane vesicles. J Biol Chem (1972) 1.36

Toward the bilayer proteome, electrospray ionization-mass spectrometry of large, intact transmembrane proteins. Proc Natl Acad Sci U S A (1999) 1.35

Molecular analysis of two fructokinases involved in sucrose metabolism of enteric bacteria. Mol Microbiol (1991) 1.35