PubRank

H Bujard

Author PubWeight™ 265.50‹?›

Top papers

Rank Title Journal Year PubWeight™‹?›
1 Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc Natl Acad Sci U S A 1992 35.45
2 Transcriptional activation by tetracyclines in mammalian cells. Science 1995 13.90
3 Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res 1997 13.15
4 Isolation of large molecular weight DNA from agarose gels for further digestion by restriction enzymes. FEBS Lett 1975 9.87
5 Organization of transcriptional signals in plasmids pBR322 and pACYC184. Proc Natl Acad Sci U S A 1981 8.77
6 Acceleration of the G1/S phase transition by expression of cyclins D1 and E with an inducible system. Mol Cell Biol 1994 7.40
7 Exploring the sequence space for tetracycline-dependent transcriptional activators: novel mutations yield expanded range and sensitivity. Proc Natl Acad Sci U S A 2000 5.99
8 Doxycycline-mediated quantitative and tissue-specific control of gene expression in transgenic mice. Proc Natl Acad Sci U S A 1996 5.93
9 Temporal control of gene expression in transgenic mice by a tetracycline-responsive promoter. Proc Natl Acad Sci U S A 1994 5.71
10 A novel in vitro transcription-translation system: accurate and efficient synthesis of single proteins from cloned DNA sequences. EMBO J 1984 4.50
11 Transcription from efficient promoters can interfere with plasmid replication and diminish expression of plasmid specified genes. EMBO J 1982 4.26
12 A T5 promoter-based transcription-translation system for the analysis of proteins in vitro and in vivo. Methods Enzymol 1987 4.21
13 Promoters largely determine the efficiency of repressor action. Proc Natl Acad Sci U S A 1988 4.18
14 Promoters of Escherichia coli: a hierarchy of in vivo strength indicates alternate structures. EMBO J 1986 3.89
15 Cloning and analysis of strong promoters is made possible by the downstream placement of a RNA termination signal. Proc Natl Acad Sci U S A 1981 3.77
16 Regulated expression of foreign genes in mammalian cells under the control of coliphage T3 RNA polymerase and lac repressor. Proc Natl Acad Sci U S A 1989 3.70
17 Physical mapping of the HindIII, EcoRI, Sal and Sma restriction endonuclease cleavage fragments from bacteriophage T5 DNA. Mol Gen Genet 1976 3.66
18 Physical interaction between heat shock proteins DnaK, DnaJ, and GrpE and the bacterial heat shock transcription factor sigma 32. Cell 1992 3.46
19 Functional dissection of Escherichia coli promoters: information in the transcribed region is involved in late steps of the overall process. EMBO J 1986 3.33
20 Co-regulation of two gene activities by tetracycline via a bidirectional promoter. Nucleic Acids Res 1995 3.24
21 Electron microscopy of size and shape of viral DNA in solutions of different ionic strengths. J Mol Biol 1967 3.16
22 Conditional expression and signaling of a specifically designed Gi-coupled receptor in transgenic mice. Nat Biotechnol 1999 3.04
23 Tetracycline-controlled transcription in eukaryotes: novel transactivators with graded transactivation potential. Nucleic Acids Res 1997 2.90
24 Control of gene activity in higher eukaryotic cells by prokaryotic regulatory elements. Trends Biochem Sci 1993 2.80
25 A chimeric transactivator allows tetracycline-responsive gene expression in whole plants. Plant J 1994 2.73
26 Tet repressor-based system for regulated gene expression in eukaryotic cells: principles and advances. Methods Enzymol 2000 2.72
27 Plasmids controlling synthesis of hemolysin in Escherichia coli: molecular properties. J Bacteriol 1974 2.71
28 RNA polymerase II transcription blocked by Escherichia coli lac repressor. Science 1990 2.65
29 Interaction of Escherichia coli RNA polymerase with promoters of several coliphage and plasmid DNAs. Proc Natl Acad Sci U S A 1979 2.58
30 A cycle of binding and release of the DnaK, DnaJ and GrpE chaperones regulates activity of the Escherichia coli heat shock transcription factor sigma32. EMBO J 1996 2.55
31 Electron microscopy of single-stranded DNA. J Mol Biol 1970 2.42
32 An electron microscopic method for studying nucleic acid-protein complexes. Visualization of RNA polymerase bound to the DNA of bacteriophages T7 and T3. Biopolymers 1974 2.24
33 Tightly regulated and inducible expression of dominant interfering dynamin mutant in stably transformed HeLa cells. Methods Enzymol 1995 2.19
34 A tetracycline controlled activation/repression system with increased potential for gene transfer into mammalian cells. J Gene Med 2000 2.18
35 lac Repressor blocks transcribing RNA polymerase and terminates transcription. Proc Natl Acad Sci U S A 1986 2.07
36 Promoter recognition and promoter strength in the Escherichia coli system. EMBO J 1987 2.07
37 Location of single-strand interruptions in the DNA of bacteriophage T5. Proc Natl Acad Sci U S A 1969 2.06
38 Electron microscopic analysis of in vitro transcriptional complexes: mapping of promoters of the coliphage T5 genome. Mol Gen Genet 1978 2.03
39 The size of the rII region of bacteriophage T4. Virology 1970 2.02
40 Subregions of a conserved part of the HIV gp41 transmembrane protein are differentially recognized by antibodies of infected individuals. EMBO J 1986 2.01
41 Promoters recognized by Escherichia coli RNA polymerase selected by function: highly efficient promoters from bacteriophage T5. J Bacteriol 1985 1.97
42 Structure and function of the genome of coliphage T5. 1. The physical structure of the chromosome of T5 + . Eur J Biochem 1973 1.90
43 Strict control of human immunodeficiency virus type 1 replication by a genetic switch: Tet for Tat. J Virol 2001 1.89
44 High susceptibility to bacterial infection, but no liver dysfunction, in mice compromised for hepatocyte NF-kappaB activation. Nat Med 2000 1.82
45 Modulation of myosin A expression by a newly established tetracycline repressor-based inducible system in Toxoplasma gondii. Nucleic Acids Res 2001 1.78
46 Inducible gene expression systems for higher eukaryotic cells. Curr Opin Biotechnol 1994 1.69
47 Polymorphism of the precursor for the major surface antigens of Plasmodium falciparum merozoites: studies at the genetic level. EMBO J 1985 1.69
48 Generation of conditional mutants in higher eukaryotes by switching between the expression of two genes. Proc Natl Acad Sci U S A 1999 1.67
49 Stalling of Escherichia coli RNA polymerase in the +6 to +12 region in vivo is associated with tight binding to consensus promoter elements. J Mol Biol 1994 1.66
50 Thrombospondin related anonymous protein (TRAP) of Plasmodium falciparum binds specifically to sulfated glycoconjugates and to HepG2 hepatoma cells suggesting a role for this molecule in sporozoite invasion of hepatocytes. EMBO J 1993 1.64
51 Isolation of covalently closed circular deoxyribonucleic acid from Streptomyces coelicolor A3(2). J Bacteriol 1975 1.63
52 Human antibody response to the major merozoite surface antigen of Plasmodium falciparum is strain specific and short-lived. Infect Immun 1991 1.55
53 Photinus pyralis luciferase: vectors that contain a modified luc coding sequence allowing convenient transfer into other systems. Gene 1994 1.54
54 Displacement of housekeeping proteasome subunits by MHC-encoded LMPs: a newly discovered mechanism for modulating the multicatalytic proteinase complex. EMBO J 1994 1.53
55 Regulation of coliphage T3 and T7 RNA polymerases by the lac repressor-operator system. Gene 1989 1.46
56 Doxycycline control of prion protein transgene expression modulates prion disease in mice. Proc Natl Acad Sci U S A 1998 1.45
57 Anhydrotetracycline, a novel effector for tetracycline controlled gene expression systems in eukaryotic cells. Nucleic Acids Res 1993 1.40
58 PL of coliphage lambda: an alternative solution for an efficient promoter. EMBO J 1988 1.38
59 Interaction of E. coli RNA polymerase with promotors of coliphage T5: the rates of complex formation and decay and their correlation with in vitro and in vivo transcriptional activity. Mol Gen Genet 1977 1.35
60 Vaccine candidate MSP-1 from Plasmodium falciparum: a redesigned 4917 bp polynucleotide enables synthesis and isolation of full-length protein from Escherichia coli and mammalian cells. Nucleic Acids Res 1999 1.34
61 Nonpolymorphic regions of p190, a protein of the Plasmodium falciparum erythrocytic stage, contain both T and B cell epitopes. J Immunol 1988 1.31
62 A prospective study of the association between the human humoral immune response to Plasmodium falciparum blood stage antigen gp190 and control of malarial infections. Infect Immun 1993 1.30
63 Electron microscopy of DNA: determination of absolute molecular weights and linear density. Mol Gen Genet 1977 1.27
64 Efficacy of tetracycline-controlled gene expression is influenced by cell type: commentary. Biotechniques 1995 1.27
65 Context-dependent effects of upstream A-tracts. Stimulation or inhibition of Escherichia coli promoter function. J Mol Biol 1994 1.24
66 Identification of mammalian cell clones exhibiting highly regulated expression from inducible promoters. Trends Genet 1995 1.23
67 Dissecting the functional program of Escherichia coli promoters: the combined mode of action of Lac repressor and AraC activator. Nucleic Acids Res 2001 1.23
68 Studies on circular DNA. II. Number of tertiary turns in papilloma DNA. J Mol Biol 1968 1.20
69 Epitopes recognized by human T cells map within the conserved part of the GP190 of P. falciparum. Science 1988 1.19
70 Structure and function of the genome of coliphage T5. 2. Regions of transcription of the chromosome. Eur J Biochem 1973 1.19
71 Long-term, noninvasive imaging of regulated gene expression in living mice. Genesis 2001 1.16
72 Development of the human immune response against the major surface protein (gp190) of Plasmodium falciparum. Infect Immun 1989 1.16
73 Efficient utilization of Escherichia coli transcriptional signals in Bacillus subtilis. J Mol Biol 1985 1.13
74 Tetracycline-regulated gene expression following direct gene transfer into mouse skeletal muscle. Somat Cell Mol Genet 1995 1.13
75 Gene therapy progress and prospects: transcription regulatory systems. Gene Ther 2004 1.10
76 Tetracycline-regulated gene expression in the brain. Curr Opin Neurobiol 2000 1.09
77 Tight control of gene expression by a helper-dependent adenovirus vector carrying the rtTA2(s)-M2 tetracycline transactivator and repressor system. Gene Ther 2002 1.05
78 Determinants for protein translocation across mammalian endoplasmic reticulum. Membrane insertion of truncated and full-length prelysozyme molecules. Eur J Biochem 1986 1.04
79 Structure and function of the genome of coliphage T5. Transcription in vitro of the "nicked" and "nick-free" T5+ DNA. Eur J Biochem 1975 0.99
80 The B lymphocyte-specific coactivator BOB.1/OBF.1 is required at multiple stages of B-cell development. Mol Cell Biol 2001 0.98
81 Doxycycline-regulated lentiviral vector system with a novel reverse transactivator rtTA2S-M2 shows a tight control of gene expression in vitro and in vivo. Gene Ther 2003 0.96
82 Use of tetracycline-controlled gene expression systems to study mammalian cell cycle. Methods Enzymol 1997 0.95
83 Plasmodium falciparum: variations within the C-terminal region of merozoite surface antigen-1. Exp Parasitol 1995 0.94
84 Analysis of the human antibody response to thrombospondin-related anonymous protein of Plasmodium falciparum. Infect Immun 1993 0.93
85 Controlling genes with tetracyclines. J Gene Med 2000 0.91
86 Membrane-associated proteases process Plasmodium falciparum merozoite surface antigen-1 (MSA1) to fragment gp41. Mol Biochem Parasitol 1992 0.90
87 Fluorinated pyrimidines. XXVII. Attempts to determine transcription errors during the formation of fluorouracil-containing messenger ribonucleic acid. Biochemistry 1966 0.90
88 Epitopes of the human malaria parasite P. falciparum carried on the surface of HBsAg particles elicit an immune response against the parasite. Vaccine 1991 0.88
89 A new tool for the serodiagnosis of acute Plasmodium falciparum malaria in individuals with primary infection. J Immunol Methods 1989 0.87
90 Regulated and prolonged expression of mIFN(alpha) in immunocompetent mice mediated by a helper-dependent adenovirus vector. Gene Ther 2001 0.84
91 Liver-specific expression of interferon gamma following adenoviral gene transfer controls hepatitis B virus replication in mice. Gene Ther 2005 0.82
92 A direct and rapid sequencing strategy for the Plasmodium falciparum antigen gene gp190/MSA1. Mol Biochem Parasitol 1995 0.82
93 Epitope analysis of human T-cell response to MSP-1 of Plasmodium falciparum in malaria-nonexposed individuals. Int Arch Allergy Immunol 1997 0.80
94 Promoter sites in the genome of B. subtilis phage SPP1. Mol Gen Genet 1981 0.80
95 Insertion of transcriptional elements outside the replication region can interfere with replication, maintenance, and stability of Co1E1-derived plasmids. Basic Life Sci 1985 0.80
96 Double-length, circular, single-stranded DNA from filamentous phage. J Virol 1974 0.78
97 The T cell reactivity against the major merozoite protein of Plasmodium falciparum. Immunol Lett 1990 0.78
98 Analysis of recombinant merozoite surface protein-1 of Plasmodium falciparum expressed in mammalian cells. Mol Biochem Parasitol 1999 0.77
99 Studies on circular deoxyribonucleic acid. I. Isolation of bovine papilloma virus and characterization of its deoxyribonucleic acid. J Virol 1967 0.76
100 [Genome structure and transcription control in bacteriophage T5+]. Hoppe Seylers Z Physiol Chem 1972 0.75
101 The human antibody response against the major merozoite surface antigen of P. falciparum is mainly directed against non-conserved sequences. Behring Inst Mitt 1991 0.75