Published in Proc Natl Acad Sci U S A on October 15, 1996
Protection of Escherichia coli cells against extreme turgor by activation of MscS and MscL mechanosensitive channels: identification of genes required for MscS activity. EMBO J (1999) 5.16
Linkage map of Escherichia coli K-12, edition 10: the traditional map. Microbiol Mol Biol Rev (1998) 4.18
Estimation of the pore size of the large-conductance mechanosensitive ion channel of Escherichia coli. Biophys J (1997) 2.98
One face of a transmembrane helix is crucial in mechanosensitive channel gating. Proc Natl Acad Sci U S A (1998) 2.89
Structural models of the MscL gating mechanism. Biophys J (2001) 2.58
Hydrophilicity of a single residue within MscL correlates with increased channel mechanosensitivity. Biophys J (1999) 2.54
Molecular architecture of full-length KcsA: role of cytoplasmic domains in ion permeation and activation gating. J Gen Physiol (2001) 2.43
Two families of mechanosensitive channel proteins. Microbiol Mol Biol Rev (2003) 2.12
Chemically charging the pore constriction opens the mechanosensitive channel MscL. Biophys J (2001) 1.81
Domain organization of the MscS mechanosensitive channel of Escherichia coli. EMBO J (2003) 1.67
Molecular dissection of the large mechanosensitive ion channel (MscL) of E. coli: mutants with altered channel gating and pressure sensitivity. J Membr Biol (1997) 1.65
Ionic regulation of MscK, a mechanosensitive channel from Escherichia coli. EMBO J (2002) 1.63
Structure of a tetrameric MscL in an expanded intermediate state. Nature (2009) 1.61
Gating of the large mechanosensitive channel in situ: estimation of the spatial scale of the transition from channel population responses. Biophys J (2004) 1.58
Conformational pathways in the gating of Escherichia coli mechanosensitive channel. Proc Natl Acad Sci U S A (2002) 1.56
Differential effects of lipids and lyso-lipids on the mechanosensitivity of the mechanosensitive channels MscL and MscS. Proc Natl Acad Sci U S A (2012) 1.44
On the conformation of the COOH-terminal domain of the large mechanosensitive channel MscL. J Gen Physiol (2003) 1.42
Molecular dynamics simulations of wild-type and mutant forms of the Mycobacterium tuberculosis MscL channel. Biophys J (2001) 1.39
Investigating lipid composition effects on the mechanosensitive channel of large conductance (MscL) using molecular dynamics simulations. Biophys J (2003) 1.36
Cysteine scanning of MscL transmembrane domains reveals residues critical for mechanosensitive channel gating. Biophys J (2004) 1.35
On the structure of the N-terminal domain of the MscL channel: helical bundle or membrane interface. Biophys J (2008) 1.33
Ion channels in microbes. Physiol Rev (2008) 1.33
Dual-color fluorescence-burst analysis to probe protein efflux through the mechanosensitive channel MscL. Biophys J (2006) 1.32
Distribution, lateral mobility and function of membrane proteins incorporated into giant unilamellar vesicles. Biophys J (2004) 1.26
Gain-of-function mutations reveal expanded intermediate states and a sequential action of two gates in MscL. J Gen Physiol (2005) 1.22
Loss-of-function mutations at the rim of the funnel of mechanosensitive channel MscL. Biophys J (2004) 1.22
A role for mechanosensitive channels in survival of stationary phase: regulation of channel expression by RpoS. Proc Natl Acad Sci U S A (2003) 1.20
Conserved motifs in mechanosensitive channels MscL and MscS. Eur Biophys J (2009) 1.20
Structure and function of the bacterial mechanosensitive channel of large conductance. Protein Sci (1999) 1.19
Gating-associated conformational changes in the mechanosensitive channel MscL. Proc Natl Acad Sci U S A (2008) 1.16
Disulfide trapping the mechanosensitive channel MscL into a gating-transition state. Biophys J (2006) 1.13
Molecular dynamics study of MscL interactions with a curved lipid bilayer. Biophys J (2006) 1.12
Molecular identification of a mechanosensitive channel in archaea. Biophys J (2001) 1.11
Defining the physical gate of a mechanosensitive channel, MscL, by engineering metal-binding sites. Biophys J (2004) 1.11
Mechanosensitive channel gating transitions resolved by functional changes upon pore modification. Biophys J (2006) 1.10
An open-pore structure of the mechanosensitive channel MscL derived by determining transmembrane domain interactions upon gating. FASEB J (2009) 1.10
Sensing and responding to membrane tension: the bacterial MscL channel as a model system. Biophys J (2012) 1.09
C-terminal charged cluster of MscL, RKKEE, functions as a pH sensor. Biophys J (2005) 1.04
Conformational state of the MscS mechanosensitive channel in solution revealed by pulsed electron-electron double resonance (PELDOR) spectroscopy. Proc Natl Acad Sci U S A (2012) 1.03
Gating mechanisms of mechanosensitive channels of large conductance, I: a continuum mechanics-based hierarchical framework. Biophys J (2008) 1.01
Electromechanical coupling model of gating the large mechanosensitive ion channel (MscL) of Escherichia coli by mechanical force. Biophys J (1998) 0.99
Single-cell census of mechanosensitive channels in living bacteria. PLoS One (2012) 0.98
Purification and functional reconstitution of N- and C-halves of the MscL channel. Biophys J (2004) 0.96
An in vivo screen reveals protein-lipid interactions crucial for gating a mechanosensitive channel. FASEB J (2010) 0.95
Common evolutionary origins of mechanosensitive ion channels in Archaea, Bacteria and cell-walled Eukarya. Archaea (2002) 0.95
The role of the periplasmic loop residue glutamine 65 for MscL mechanosensitivity. Eur Biophys J (2005) 0.94
Hydrophobic gating of mechanosensitive channel of large conductance evidenced by single-subunit resolution. Proc Natl Acad Sci U S A (2012) 0.93
Three routes to modulate the pore size of the MscL channel/nanovalve. ACS Nano (2012) 0.93
Expression and characterization of the bacterial mechanosensitive channel MscS in Xenopus laevis oocytes. J Gen Physiol (2011) 0.92
Structural investigation of MscL gating using experimental data and coarse grained MD simulations. PLoS Comput Biol (2012) 0.91
Visualisation of the mechanosensitive channel of large conductance in bacteria using confocal microscopy. Eur Biophys J (2005) 0.90
Identification of mutations that alter the gating of the Escherichia coli mechanosensitive channel protein, MscK. Mol Microbiol (2007) 0.90
Droplet interface bilayer reconstitution and activity measurement of the mechanosensitive channel of large conductance from Escherichia coli. J R Soc Interface (2014) 0.90
Tryptophan in the pore of the mechanosensitive channel MscS: assessment of pore conformations by fluorescence spectroscopy. J Biol Chem (2009) 0.89
Phosphatidylinositol is crucial for the mechanosensitivity of Mycobacterium tuberculosis MscL. Biochemistry (2013) 0.89
A new antibiotic with potent activity targets MscL. J Antibiot (Tokyo) (2015) 0.88
The gating mechanism of the bacterial mechanosensitive channel MscL revealed by molecular dynamics simulations: from tension sensing to channel opening. Channels (Austin) (2012) 0.88
Tension-activated channels in the mechanism of osmotic fitness in Pseudomonas aeruginosa. J Gen Physiol (2017) 0.86
Streptomycin potency is dependent on MscL channel expression. Nat Commun (2014) 0.86
The activation mode of the mechanosensitive ion channel, MscL, by lysophosphatidylcholine differs from tension-induced gating. FASEB J (2014) 0.85
The mechanosensitive channel of small conductance (MscS) functions as a Jack-in-the box. Biochim Biophys Acta (2014) 0.85
Manipulating the permeation of charged compounds through the MscL nanovalve. FASEB J (2010) 0.85
The role of MscL amphipathic N terminus indicates a blueprint for bilayer-mediated gating of mechanosensitive channels. Nat Commun (2016) 0.85
Chimeras reveal a single lipid-interface residue that controls MscL channel kinetics as well as mechanosensitivity. Cell Rep (2013) 0.85
Membrane tension, lipid adaptation, conformational changes, and energetics in MscL gating. Biophys J (2011) 0.85
Improving the Design of a MscL-Based Triggered Nanovalve. Biosensors (Basel) (2013) 0.84
Translocation of alpha-synuclein expressed in Escherichia coli. J Bacteriol (2007) 0.84
Force transduction and lipid binding in MscL: a continuum-molecular approach. PLoS One (2014) 0.84
The molecular basis of mechanosensory transduction. Adv Exp Med Biol (2012) 0.83
Engineering covalent oligomers of the mechanosensitive channel of large conductance from Escherichia coli with native conductance and gating characteristics. Protein Sci (2005) 0.83
The dynamics of protein-protein interactions between domains of MscL at the cytoplasmic-lipid interface. Channels (Austin) (2012) 0.83
MscL: channeling membrane tension. Pflugers Arch (2014) 0.83
Structure and stability of the C-terminal helical bundle of the E. coli mechanosensitive channel of large conductance. Protein Sci (2013) 0.83
Mechanical coupling of the multiple structural elements of the large-conductance mechanosensitive channel during expansion. Proc Natl Acad Sci U S A (2015) 0.80
Confirming the revised C-terminal domain of the MscL crystal structure. Biophys J (2008) 0.79
Dynamics of protein-protein interactions at the MscL periplasmic-lipid interface. Biophys J (2014) 0.79
Functional similarities between heterogeneously and homogenously expressed MscL constructs. Eur Biophys J (2015) 0.78
Electrostatics at the membrane define MscL channel mechanosensitivity and kinetics. FASEB J (2014) 0.77
Scanning MscL Channels with Targeted Post-Translational Modifications for Functional Alterations. PLoS One (2015) 0.77
Studying mechanosensitive ion channels with an automated patch clamp. Eur Biophys J (2014) 0.77
Mutations in a Conserved Domain of E. coli MscS to the Most Conserved Superfamily Residue Leads to Kinetic Changes. PLoS One (2015) 0.76
Gating of a mechanosensitive channel due to cellular flows. Proc Natl Acad Sci U S A (2015) 0.76
From membrane tension to channel gating: A principal energy transfer mechanism for mechanosensitive channels. Protein Sci (2016) 0.76
Dihydrostreptomycin Directly Binds to, Modulates, and Passes through the MscL Channel Pore. PLoS Biol (2016) 0.75
On the mobility, membrane location and functionality of mechanosensitive channels in Escherichia coli. Sci Rep (2016) 0.75
Properties of the Mechanosensitive Channel MscS Pore Revealed by Tryptophan Scanning Mutagenesis. Biochemistry (2015) 0.75
Oncogenic Mutations Differentially Affect Bax Monomer, Dimer, and Oligomeric Pore Formation in the Membrane. Sci Rep (2016) 0.75
Sensory Neuron-Specific Deletion of TRPA1 Results in Mechanical Cutaneous Sensory Deficits. eNeuro (2017) 0.75
Nonchemotactic mutants of Escherichia coli. J Bacteriol (1967) 13.70
A large-conductance mechanosensitive channel in E. coli encoded by mscL alone. Nature (1994) 5.46
Pressure-sensitive ion channel in Escherichia coli. Proc Natl Acad Sci U S A (1987) 5.06
Mechanosensitive ion channels of E. coli activated by amphipaths. Nature (1990) 3.55
Two types of mechanosensitive channels in the Escherichia coli cell envelope: solubilization and functional reconstitution. Biophys J (1993) 2.53
Gadolinium ion inhibits loss of metabolites induced by osmotic shock and large stretch-activated channels in bacteria. Eur J Biochem (1992) 2.44
Purification and functional reconstitution of the recombinant large mechanosensitive ion channel (MscL) of Escherichia coli. J Biol Chem (1995) 2.43
Modified reconstitution method used in patch-clamp studies of Escherichia coli ion channels. Biophys J (1989) 2.25
Characterization of mechanosensitive channels in Escherichia coli cytoplasmic membrane by whole-cell patch clamp recording. J Membr Biol (1995) 1.53
Oligodeoxynucleotide-directed mutagenesis of Escherichia coli and yeast by simple cotransformation of the primer and template. DNA (1986) 1.51
Fusion of bacterial spheroplasts by electric fields. Biochim Biophys Acta (1985) 1.43
Effect of outer membrane permeability on chemotaxis in Escherichia coli. J Bacteriol (1990) 1.39
Mutational analysis of muscle nicotinic acetylcholine receptor subunit assembly. J Cell Biol (1990) 1.34
Ion-conducting channels in a gram-positive bacterium. FEBS Lett (1988) 1.28
A patch-clamp investigation of the Streptococcus faecalis cell membrane. J Membr Biol (1993) 1.13
A patch-clamp study of Bacillus subtilis. Biochim Biophys Acta (1992) 1.07
Stretch-activated composite ion channels in Bacillus subtilis. Biochem Biophys Res Commun (1990) 1.07
A large-conductance mechanosensitive channel in E. coli encoded by mscL alone. Nature (1994) 5.46
Pressure-sensitive ion channel in Escherichia coli. Proc Natl Acad Sci U S A (1987) 5.06
Energetic and spatial parameters for gating of the bacterial large conductance mechanosensitive channel, MscL. J Gen Physiol (1999) 4.10
Mechanosensitive ion channels of E. coli activated by amphipaths. Nature (1990) 3.55
One face of a transmembrane helix is crucial in mechanosensitive channel gating. Proc Natl Acad Sci U S A (1998) 2.89
Mechanosensitive channels of Escherichia coli: the MscL gene, protein, and activities. Annu Rev Physiol (1997) 2.84
Membrane topology and multimeric structure of a mechanosensitive channel protein of Escherichia coli. EMBO J (1996) 2.81
Ion channels in yeast. Science (1986) 2.75
A mechanosensitive ion channel in the yeast plasma membrane. Science (1988) 2.68
COT1, a gene involved in cobalt accumulation in Saccharomyces cerevisiae. Mol Cell Biol (1992) 2.62
Hydrophilicity of a single residue within MscL correlates with increased channel mechanosensitivity. Biophys J (1999) 2.54
Two types of mechanosensitive channels in the Escherichia coli cell envelope: solubilization and functional reconstitution. Biophys J (1993) 2.53
The electrical breakdown of cell and lipid membranes: the similarity of phenomenologies. Biochim Biophys Acta (1987) 2.41
Design of allele-specific inhibitors to probe protein kinase signaling. Curr Biol (1998) 2.36
Genetic modification of electric properties in an excitable membrane (paramecium-calcium conductance-electrophysiological measurements-membrane mutant). Proc Natl Acad Sci U S A (1972) 2.31
Modified reconstitution method used in patch-clamp studies of Escherichia coli ion channels. Biophys J (1989) 2.25
Mechanosensitive channels of bacteria. Methods Enzymol (1999) 2.23
Genetic dissection of behavior in paramecium. Science (1975) 2.12
Mutations in a bacterial mechanosensitive channel change the cellular response to osmotic stress. J Biol Chem (1997) 2.06
Functional and structural conservation in the mechanosensitive channel MscL implicates elements crucial for mechanosensation. Mol Microbiol (1998) 2.00
Genic mutants with altered system of excitation in Paramecium aurelia. II. Mutagenesis, screening and genetic analysis of the mutants. Genetics (1971) 1.84
Chemically charging the pore constriction opens the mechanosensitive channel MscL. Biophys J (2001) 1.81
Electrically induced DNA uptake by cells is a fast process involving DNA electrophoresis. Biophys J (1991) 1.81
Biochemical studies of the excitable membrane of Paramecium tetraurelia. III. Proteins of cilia and ciliary membranes. J Cell Biol (1980) 1.78
Towards an understanding of the structural and functional properties of MscL, a mechanosensitive channel in bacteria. Biol Cell (1996) 1.77
Separation of membrane currents using a Paramecium mutant. Nature (1977) 1.73
A mechanosensitive channel in whole cells and in membrane patches of the fungus Uromyces. Science (1991) 1.72
A TRP homolog in Saccharomyces cerevisiae forms an intracellular Ca(2+)-permeable channel in the yeast vacuolar membrane. Proc Natl Acad Sci U S A (2001) 1.63
Interactions between gene products involved in divalent cation transport in Saccharomyces cerevisiae. Mol Gen Genet (1994) 1.61
Yeast casein kinase I homologues: an essential gene pair. Proc Natl Acad Sci U S A (1992) 1.60
Rotation and twist of the central-pair microtubules in the cilia of Paramecium. J Cell Biol (1980) 1.45
Mutations in paramecium calmodulin indicate functional differences between the C-terminal and N-terminal lobes in vivo. Cell (1990) 1.42
The physiological basis of taxes in Paramecium. Annu Rev Physiol (1982) 1.40
The COT2 gene is required for glucose-dependent divalent cation transport in Saccharomyces cerevisiae. Mol Cell Biol (1993) 1.36
Temperature-sensitive pawns: conditional behavioral mutants of Paramecium aurelia. Science (1973) 1.36
Random mutagenesis reveals a region important for gating of the yeast K+ channel Ykc1. EMBO J (1997) 1.34
The pair of central tubules rotates during ciliary beat in Paramecium. Nature (1979) 1.33
Calcium-induced ciliary reversal in the extracted models of "Pawn", a behavioral mutant of Paramecium. Science (1973) 1.32
Restoration by calmodulin of a Ca2+-dependent K+ current missing in a mutant of Paramecium. Science (1986) 1.30
Stoichiometry of the large conductance bacterial mechanosensitive channel of E. coli. A biochemical study. J Membr Biol (1999) 1.30
Genetic dissection of active electrogenesis in Paramecium aurelia. Nature (1974) 1.29
Manganese effectively supports yeast cell-cycle progression in place of calcium. J Cell Biol (1995) 1.28
Are ions involved in the gating of calcium channels? Science (1982) 1.22
Electrical properties of cell pellets and cell electrofusion in a centrifuge. Biochim Biophys Acta (1993) 1.22
Yeast K1 killer toxin forms ion channels in sensitive yeast spheroplasts and in artificial liposomes. Proc Natl Acad Sci U S A (1990) 1.19
Mutant analysis shows that the Ca2+-induced K+ current shuts off one type of excitation in Paramecium. Proc Natl Acad Sci U S A (1983) 1.19
YKC1 encodes the depolarization-activated K+ channel in the plasma membrane of yeast. FEBS Lett (1995) 1.16
Defective ion regulation in a class of membrane-excitation mutants in Paramecium. Biochim Biophys Acta (1976) 1.14
Osmotaxis in Escherichia coli. Proc Natl Acad Sci U S A (1988) 1.13
A Ca-induced Na-current in Paramecium. J Exp Biol (1980) 1.12
Helicity, membrane incorporation, orientation and thermal stability of the large conductance mechanosensitive ion channel from E. coli. Biochim Biophys Acta (1998) 1.12
Microinjection of cytoplasm as a test of complementation in Paramecium. J Cell Biol (1982) 1.10
Behavioral genetics of Paramecium. Annu Rev Genet (1987) 1.09
Ultrastructure of the proximal region of somatic cilia in Paramecium tetraurelia. J Cell Biol (1978) 1.08
Induction of antibiotic resistance in Paramecium tetraurelia by the bacterial gene APH-3'-II. J Eukaryot Microbiol (1995) 1.08
A mutant Paramecium with a defective calcium-dependent potassium conductance has an altered calmodulin: a nonlethal selective alteration in calmodulin regulation. Proc Natl Acad Sci U S A (1987) 1.08
Membrane currents of pawn mutants of the pwA group in Paramecium tetraurelia. J Exp Biol (1980) 1.06
Definition of family of coronin-related proteins conserved between humans and mice: close genetic linkage between coronin-2 and CD45-associated protein. DNA Cell Biol (1998) 1.05
Membrane-derived oligosaccharides (MDO's) promote closing of an E. coli porin channel. FEBS Lett (1992) 1.04
Voltage-dependent calcium channels from Paramecium cilia incorporated into planar lipid bilayers. Science (1984) 1.03
Ca-induced K+-outward current in Paramecium tetraurelia. J Exp Biol (1980) 1.03
Calmodulin mutants and Ca2(+)-dependent channels in Paramecium. Annu Rev Physiol (1991) 1.03
Ion channels in Paramecium, yeast and Escherichia coli. Trends Biochem Sci (1988) 1.03
Ion channel activities in the Escherichia coli outer membrane. Biochim Biophys Acta (1990) 1.02
Structure of Paramecium tetraurelia calmodulin at 1.8 A resolution. Protein Sci (1993) 1.02
Genetic analyses of "paranoiac" mutants of Paramecium tetraurelia. Genetics (1977) 1.02
Paramecium genome survey: a pilot project. Trends Genet (2001) 1.02
Channel gate! Tension, leak and disclosure. Structure (1999) 1.01
Characterization and purification of a soluble protein controlling Ca-channel activity in paramecium. Cell (1984) 1.00
Patch clamp studies of microbial ion channels. Methods Enzymol (1992) 1.00
Mutants with reduced Ca activation in Paramecium aurelia. J Membr Biol (1976) 0.99
Mutants with altered Ca2+-channel properties in Paramecium tetraurelia: isolation, characterization and genetic analysis. Genetics (1984) 0.98
Studies of the cell surface of Paramecium. Ciliary membrane proteins and immobilization antigens. Biochem J (1975) 0.98
Selection and analysis of a mutant Paramecium tetraurelia lacking behavioural response to tetraethylammonium. Genet Res (1976) 0.98
Intra- and interspecific complementation of membrane-inexcitable mutants of Paramecium. J Cell Biol (1983) 0.98
An extensive behavioural and genetic analysis of the pawn mutants in Paramecium aurelia. Genet Res (1974) 0.97
Voltage-sensitive ion channel of Escherichia coli. J Membr Biol (1989) 0.96
A 'TEA+-insensitive' mutant with increased potassium conductance in Paramecium aurelia. J Exp Biol (1976) 0.96
Genetic Analyses of Heat-Sensitve Pawn Mutants of PARAMECIUM AURELIA. Genetics (1973) 0.96
Osmotaxis in Escherichia coli. Cold Spring Harb Symp Quant Biol (1988) 0.96
The cloning and molecular analysis of pawn-B in Paramecium tetraurelia. Genetics (2000) 0.96
A single amino acid substitution alters conductance and gating of OmpC porin of Escherichia coli. J Membr Biol (1991) 0.95
A comparison of internal eliminated sequences in the genes that encode two K(+)-channel isoforms in Paramecium tetraurelia. J Eukaryot Microbiol (1998) 0.95
Calcium current activated upon hyperpolarization of Paramecium tetraurelia. J Gen Physiol (1992) 0.94
The effect of sodium on "paranoiac"- a membrane mutant of Paramecium. Comp Biochem Physiol A Comp Physiol (1976) 0.94
A mutant of Paramecium with increased relative resting potassium permeability. J Neurobiol (1976) 0.93
In vivo mutations of calmodulin: a mutant Paramecium with altered ion current regulation has an isoleucine-to-threonine change at residue 136 and an altered methylation state at lysine residue 115. Proc Natl Acad Sci U S A (1989) 0.91
Genetic analysis of mutants with a reduced Ca2+-dependent K+ current in Paramecium tetraurelia. Genetics (1985) 0.91
Calmodulin defects cause the loss of Ca2(+)-dependent K+ currents in two pantophobiac mutants of Paramecium tetraurelia. J Membr Biol (1990) 0.91
Efficient transformation of cam2, a behavioral mutant of Paramecium tetraurelia, with the calmodulin gene. Proc Natl Acad Sci U S A (1991) 0.91
Slow inactivation of the calcium current of Paramecium is dependent on voltage and not internal calcium. J Physiol (1985) 0.91
Efficient expression of the Paramecium calmodulin gene in Escherichia coli after four TAA-to-CAA changes through a series of polymerase chain reactions. J Protozool (1991) 0.90
Mutational alteration of membrane phospholipid composition and voltage-sensitive ion channel function in paramecium. Proc Natl Acad Sci U S A (1981) 0.90