Published in Protein Sci on October 01, 1997
Determining selectivity of phosphoinositide-binding domains. Methods (2006) 1.62
Dimerization and N-terminal domain proximity underlie the function of the molecular chaperone heat shock protein 90. Proc Natl Acad Sci U S A (2000) 1.53
Cross-species binding analyses of mouse and human neonatal Fc receptor show dramatic differences in immunoglobulin G and albumin binding. J Biol Chem (2009) 1.44
Specific protein domains mediate cooperative assembly of HuR oligomers on AU-rich mRNA-destabilizing sequences. J Biol Chem (2007) 1.41
C2 domains from different Ca2+ signaling pathways display functional and mechanistic diversity. Biochemistry (2001) 1.33
A kinase-regulated mechanism controls CFTR channel gating by disrupting bivalent PDZ domain interactions. Proc Natl Acad Sci U S A (2003) 1.31
A thermodynamic ligand binding study of the third PDZ domain (PDZ3) from the mammalian neuronal protein PSD-95. Biochemistry (2007) 1.25
A membrane proximal region of the integrin alpha5 subunit is important for its interaction with nischarin. Biochem J (2004) 0.97
Roles of two large serine recombinases in mobilizing the methicillin-resistance cassette SCCmec. Mol Microbiol (2013) 0.95
Nuclear export and plasma membrane recruitment of the Ste5 scaffold are coordinated with oligomerization and association with signal transduction components. Mol Biol Cell (2003) 0.95
The Dbs PH domain contributes independently to membrane targeting and regulation of guanine nucleotide-exchange activity. Biochem J (2006) 0.95
The maltose-binding protein as a scaffold for monovalent display of peptides derived from phage libraries. Anal Biochem (1998) 0.95
Protein kinase A regulates ATP hydrolysis and dimerization by a CFTR (cystic fibrosis transmembrane conductance regulator) domain. Biochem J (2004) 0.91
Isolation of receptor-ligand pairs by capture of long-lived multivalent interaction complexes. Proc Natl Acad Sci U S A (2002) 0.87
Interactions between DNA, transcriptional regulator Dreb2a and the Med25 mediator subunit from Arabidopsis thaliana involve conformational changes. Nucleic Acids Res (2012) 0.87
A novel TATA-binding protein-binding protein, ABT1, activates basal transcription and has a yeast homolog that is essential for growth. Mol Cell Biol (2000) 0.83
Maltose-binding protein enhances secretion of recombinant human granzyme B accompanied by in vivo processing of a precursor MBP fusion protein. PLoS One (2010) 0.81
Recombinant production of the amino terminal cytoplasmic region of dengue virus non-structural protein 4A for structural studies. PLoS One (2014) 0.78
Chaperone activity of human small heat shock protein-GST fusion proteins. Cell Stress Chaperones (2017) 0.75
Expression and Structural Analyses of Human DNA Polymerase θ (POLQ). Methods Enzymol (2017) 0.75
Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene (1985) 213.67
Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem (1989) 29.15
Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem (1974) 27.82
Peptide and protein molecular weight determination by electrophoresis using a high-molarity tris buffer system without urea. Anal Biochem (1986) 4.83
Use of the tetracycline promoter for the tightly regulated production of a murine antibody fragment in Escherichia coli. Gene (1994) 2.85
Covalent chromatography. Preparation of fully active papain from dried papaya latex. Biochem J (1973) 2.79
The influence of polyvalency on the binding properties of antibodies. Immunochemistry (1972) 2.67
The 2.0 A X-ray crystal structure of chicken egg white cystatin and its possible mode of interaction with cysteine proteinases. EMBO J (1988) 2.48
Cystatin, a protein inhibitor of cysteine proteinases. Improved purification from egg white, characterization, and detection in chicken serum. Biochem J (1983) 2.42
Three-dimensional structure of Schistosoma japonicum glutathione S-transferase fused with a six-amino acid conserved neutralizing epitope of gp41 from HIV. Protein Sci (1994) 1.91
Mutagenesis of a flexible loop in streptavidin leads to higher affinity for the Strep-tag II peptide and improved performance in recombinant protein purification. Protein Eng (1997) 1.85
One-step affinity purification of bacterially produced proteins by means of the "Strep tag" and immobilized recombinant core streptavidin. J Chromatogr A (1994) 1.57
The random peptide library-assisted engineering of a C-terminal affinity peptide, useful for the detection and purification of a functional Ig Fv fragment. Protein Eng (1993) 1.54
Miniantibodies: use of amphipathic helices to produce functional, flexibly linked dimeric FV fragments with high avidity in Escherichia coli. Biochemistry (1992) 1.46
Biochemical properties of cloned glutathione S-transferases from Schistosoma mansoni and Schistosoma japonicum. Mol Biochem Parasitol (1993) 1.44
Disuccinimidyl esters as bifunctional crosslinking reagents for proteins: assays with myosin. FEBS Lett (1979) 1.14
Effect of redox environment on the in vitro and in vivo folding of RTEM-1 beta-lactamase and Escherichia coli alkaline phosphatase. J Biol Chem (1994) 1.07
Characterization by rapid-kinetic and equilibrium methods of the interaction between N-terminally truncated forms of chicken cystatin and the cysteine proteinases papain and actinidin. Biochem J (1992) 0.95
Cytoplasmic and periplasmic production of human placental glutathione transferase in Escherichia coli. Protein Expr Purif (1995) 0.89
The rational construction of an antibody against cystatin: lessons from the crystal structure of an artificial Fab fragment. J Mol Biol (1997) 0.86
Recombinant colorimetric antibodies: construction and characterization of a bifunctional F(ab)2/alkaline phosphatase conjugate produced in Escherichia coli. Biotechnology (N Y) (1993) 0.85
Purification and characterization of a chicken egg white cystatin variant expressed in an Escherichia coli pIN-III-ompA system. Eur J Biochem (1991) 0.83
Synthesis of a (desSer1 Ile29 Leu89) chicken cystatin gene, expression in E. coli as fusion protein and its isolation. FEBS Lett (1989) 0.80
Structure and dynamics of one-dimensional ionic solutions in biological transmembrane channels. Biophys J (1987) 2.10
Molecular interaction between the Strep-tag affinity peptide and its cognate target, streptavidin. J Mol Biol (1996) 2.10
Mutagenesis of a flexible loop in streptavidin leads to higher affinity for the Strep-tag II peptide and improved performance in recombinant protein purification. Protein Eng (1997) 1.85
One-step affinity purification of bacterially produced proteins by means of the "Strep tag" and immobilized recombinant core streptavidin. J Chromatogr A (1994) 1.57
The random peptide library-assisted engineering of a C-terminal affinity peptide, useful for the detection and purification of a functional Ig Fv fragment. Protein Eng (1993) 1.54
Regeneration of lesioned corticospinal tract fibers in the adult rat induced by a recombinant, humanized IN-1 antibody fragment. J Neurosci (2000) 1.45
Simulation of voltage-driven hydrated cation transport through narrow transmembrane channels. Biophys J (1987) 1.34
Small antibody-like proteins with prescribed ligand specificities derived from the lipocalin fold. Proc Natl Acad Sci U S A (1999) 1.34
Application of neutralizing antibodies against NI-35/250 myelin-associated neurite growth inhibitory proteins to the adult rat cerebellum induces sprouting of uninjured purkinje cell axons. J Neurosci (2000) 1.20
Ultrafast electron transfer in the complex between fluorescein and a cognate engineered lipocalin protein, a so-called anticalin. Biochemistry (2002) 1.12
Engineered Fv fragments as a tool for the one-step purification of integral multisubunit membrane protein complexes. Biotechnology (N Y) (1995) 1.05
Immobilization of histidine-tagged proteins on gold surfaces using chelator thioalkanes. Biosens Bioelectron (1999) 1.02
An engineered lipocalin specific for CTLA-4 reveals a combining site with structural and conformational features similar to antibodies. Proc Natl Acad Sci U S A (2009) 1.01
Expression of functional antibody Fv and Fab fragments in Escherichia coli. Methods Enzymol (1989) 0.97
Grafting of a high-affinity Zn(II)-binding site on the beta-barrel of retinol-binding protein results in enhanced folding stability and enables simplified purification. Biochemistry (1994) 0.92
Bacterially produced apolipoprotein D binds progesterone and arachidonic acid, but not bilirubin or E-3M2H. J Mol Recognit (2001) 0.92
A novel type of receptor protein, based on the lipocalin scaffold, with specificity for digoxigenin. J Mol Biol (2000) 0.92
An engineered IN-1 F(ab) fragment with improved affinity for the Nogo-A axonal growth inhibitor permits immunochemical detection and shows enhanced neutralizing activity. Protein Eng (2002) 0.90
Bifunctional and multimeric complexes of streptavidin fused to single chain antibodies (scFv). J Immunol Methods (1995) 0.89
Rapid expression cloning of human immunoglobulin Fab fragments for the analysis of antigen specificity of B cell lymphomas and anti-idiotype lymphoma vaccination. J Immunol Methods (1999) 0.89
Colony assays for antibody fragments expressed in bacteria. J Immunol Methods (1991) 0.87
Retrograde regulation of growth-associated gene expression in adult rat Purkinje cells by myelin-associated neurite growth inhibitory proteins. J Neurosci (1998) 0.86
The rational construction of an antibody against cystatin: lessons from the crystal structure of an artificial Fab fragment. J Mol Biol (1997) 0.86
The bilin-binding protein of Pieris brassicae. cDNA sequence and regulation of expression reveal distinct features of this insect pigment protein. Eur J Biochem (1994) 0.86
Sequence analysis and bacterial production of the anti-c-myc antibody 9E10: the V(H) domain has an extended CDR-H3 and exhibits unusual solubility. FEBS Lett (1997) 0.85
Intracellular expression of a cloned antibody fragment interferes with hepatitis B virus surface antigen secretion. Biochem Biophys Res Commun (1999) 0.84
Fermenter production of an artificial fab fragment, rationally designed for the antigen cystatin, and its optimized crystallization through constant domain shuffling. Proteins (1995) 0.83
Use of an albumin-binding domain for the selective immobilisation of recombinant capture antibody fragments on ELISA plates. J Immunol Methods (1998) 0.83
Functional expression of the uncomplexed serum retinol-binding protein in Escherichia coli. Ligand binding and reversible unfolding characteristics. J Mol Biol (1993) 0.82
Improved folding of apo-retinol-binding protein in the periplasm of Escherichia coli: positive influences of dsbC coexpression and of an amino acid exchange in the vitamin A binding site. Protein Eng (1998) 0.82
The de novo design of an antibody combining site. Crystallographic analysis of the VL domain confirms the structural model. J Mol Biol (1994) 0.82
Single-step purification of a bacterially expressed antibody Fv fragment by immobilized metal affinity chromatography in the presence of betaine. J Chromatogr A (1993) 0.80
The Escherichia coli-derived Fab fragment of the IgM/kappa antibody IN-1 recognizes and neutralizes myelin-associated inhibitors of neurite growth. Eur J Biochem (1996) 0.80
A Zn(II)-binding site engineered into retinol-binding protein exhibits metal-ion specificity and allows highly efficient affinity purification with a newly designed metal ligand. Chem Biol (1996) 0.79
Duocalins: engineered ligand-binding proteins with dual specificity derived from the lipocalin fold. Biol Chem (2001) 0.78
Use of thiophilic adsorption chromatography for the one-step purification of a bacterially produced antibody F(ab) fragment without the need for an affinity tag. Protein Expr Purif (1999) 0.78
Engineering of antibodies with a known three-dimensional structure. Cold Spring Harb Symp Quant Biol (1987) 0.78
Investigation of a tetracycline-regulated phage display system. Protein Eng (1999) 0.77
Properties of FV and Fab fragments of the antibody McPC603 expressed in E. coli. Behring Inst Mitt (1990) 0.76
Regeneration of auditory nerve following complete sectioning and intrathecal application of the IN-1 antibody. Acta Neurochir (Wien) (2002) 0.75