Published in J Biol Chem on August 21, 1998
Different modes of SecY-SecA interactions revealed by site-directed in vivo photo-cross-linking. Proc Natl Acad Sci U S A (2006) 1.61
SecM facilitates translocase function of SecA by localizing its biosynthesis. Genes Dev (2005) 1.57
Dimeric SecA is essential for protein translocation. Proc Natl Acad Sci U S A (2005) 1.39
Role of lipids in the translocation of proteins across membranes. Biochem J (2000) 1.04
Roles of SecG in ATP- and SecA-dependent protein translocation. Proc Natl Acad Sci U S A (1998) 1.03
Competitive binding of the SecA ATPase and ribosomes to the SecYEG translocon. J Biol Chem (2012) 0.90
The SecA subunit of Escherichia coli preprotein translocase is exposed to the periplasm. J Bacteriol (1998) 0.89
Phospholipids induce conformational changes of SecA to form membrane-specific domains: AFM structures and implication on protein-conducting channels. PLoS One (2013) 0.81
The variable subdomain of Escherichia coli SecA functions to regulate SecA ATPase activity and ADP release. J Bacteriol (2012) 0.78
Design, Synthesis and Evaluation of Triazole-Pyrimidine Analogues as SecA Inhibitors. ChemMedChem (2015) 0.76
SecA functions in vivo as a discrete anti-parallel dimer to promote protein transport. Mol Microbiol (2016) 0.75
RNA synthesis initiates in vitro conversion of M13 DNA to its replicative form. Proc Natl Acad Sci U S A (1972) 8.09
Initiation of DNA synthesis: synthesis of phiX174 replicative form requires RNA synthesis resistant to rifampicin. Proc Natl Acad Sci U S A (1972) 5.74
Sec18p (NSF)-driven release of Sec17p (alpha-SNAP) can precede docking and fusion of yeast vacuoles. Cell (1996) 5.18
Conserved residues of the leader peptide are essential for cleavage by leader peptidase. J Biol Chem (1985) 4.89
The purified E. coli integral membrane protein SecY/E is sufficient for reconstitution of SecA-dependent precursor protein translocation. Cell (1990) 4.78
The assembly of proteins into biological membranes: The membrane trigger hypothesis. Annu Rev Biochem (1979) 4.05
The ATPase activity of SecA is regulated by acidic phospholipids, SecY, and the leader and mature domains of precursor proteins. Cell (1990) 4.00
SecA promotes preprotein translocation by undergoing ATP-driven cycles of membrane insertion and deinsertion. Cell (1994) 3.95
The GTPase Ypt7p of Saccharomyces cerevisiae is required on both partner vacuoles for the homotypic fusion step of vacuole inheritance. EMBO J (1995) 3.76
The binding cascade of SecB to SecA to SecY/E mediates preprotein targeting to the E. coli plasma membrane. Cell (1990) 3.58
Defining the functions of trans-SNARE pairs. Nature (1998) 3.52
Multiple methods of visualizing the yeast vacuole permit evaluation of its morphology and inheritance during the cell cycle. J Cell Biol (1987) 3.48
A vacuolar v-t-SNARE complex, the predominant form in vivo and on isolated vacuoles, is disassembled and activated for docking and fusion. J Cell Biol (1998) 3.44
Leader peptidase catalyzes the release of exported proteins from the outer surface of the Escherichia coli plasma membrane. J Biol Chem (1985) 3.35
SecA protein hydrolyzes ATP and is an essential component of the protein translocation ATPase of Escherichia coli. EMBO J (1989) 3.33
Sequence of the leader peptidase gene of Escherichia coli and the orientation of leader peptidase in the bacterial envelope. J Biol Chem (1983) 3.22
Synthesis, assembly into the cytoplasmic membrane, and proteolytic processing of the precursor of coliphage M13 coat protein. J Biol Chem (1980) 3.09
Delta mu H+ and ATP function at different steps of the catalytic cycle of preprotein translocase. Cell (1991) 2.98
Trigger factor: a soluble protein that folds pro-OmpA into a membrane-assembly-competent form. Proc Natl Acad Sci U S A (1987) 2.97
Organelle inheritance. Cell (1996) 2.96
Assembly of proteins into membranes. Science (1980) 2.92
Three pure chaperone proteins of Escherichia coli--SecB, trigger factor and GroEL--form soluble complexes with precursor proteins in vitro. EMBO J (1989) 2.89
Asymmetric orientation of phage M13 coat protein in Escherichia coli cytoplasmic membranes and in synthetic lipid vesicles. Proc Natl Acad Sci U S A (1976) 2.84
Purification and characterization of leader (signal) peptidase from Escherichia coli. J Biol Chem (1980) 2.76
Distinct catalytic roles of the SecYE, SecG and SecDFyajC subunits of preprotein translocase holoenzyme. EMBO J (1997) 2.60
SecA membrane cycling at SecYEG is driven by distinct ATP binding and hydrolysis events and is regulated by SecD and SecF. Cell (1995) 2.58
Homotypic vacuole fusion requires Sec17p (yeast alpha-SNAP) and Sec18p (yeast NSF). EMBO J (1996) 2.52
Docking of yeast vacuoles is catalyzed by the Ras-like GTPase Ypt7p after symmetric priming by Sec18p (NSF). J Cell Biol (1997) 2.51
Mechanisms of membrane assembly: effects of energy poisons on the conversion of soluble M13 coliphage procoat to membrane-bound coat protein. Proc Natl Acad Sci U S A (1980) 2.51
G-protein ligands inhibit in vitro reactions of vacuole inheritance. J Cell Biol (1994) 2.51
ProOmpA is stabilized for membrane translocation by either purified E. coli trigger factor or canine signal recognition particle. Cell (1988) 2.42
A new form of DNA polymerase 3 and a copolymerase replicate a long, single-stranded primer-template. Proc Natl Acad Sci U S A (1973) 2.42
Effects of two sec genes on protein assembly into the plasma membrane of Escherichia coli. J Biol Chem (1985) 2.40
Use of phoA fusions to study the topology of the Escherichia coli inner membrane protein leader peptidase. J Bacteriol (1989) 2.38
SecA protein, a peripheral protein of the Escherichia coli plasma membrane, is essential for the functional binding and translocation of proOmpA. EMBO J (1989) 2.33
Three v-SNAREs and two t-SNAREs, present in a pentameric cis-SNARE complex on isolated vacuoles, are essential for homotypic fusion. J Cell Biol (1999) 2.31
In vitro reactions of vacuole inheritance in Saccharomyces cerevisiae. J Cell Biol (1992) 2.28
Both ATP and the electrochemical potential are required for optimal assembly of pro-OmpA into Escherichia coli inner membrane vesicles. Proc Natl Acad Sci U S A (1986) 2.16
The docking stage of yeast vacuole fusion requires the transfer of proteins from a cis-SNARE complex to a Rab/Ypt protein. J Cell Biol (2000) 2.13
The isolation of homogeneous leader peptidase from a strain of Escherichia coli which overproduces the enzyme. J Biol Chem (1982) 2.11
ProOmpA spontaneously folds in a membrane assembly competent state which trigger factor stabilizes. EMBO J (1988) 2.10
Intervacuole exchange in the yeast zygote: a new pathway in organelle communication. Science (1988) 2.06
Soluble precursor of an integral membrane protein: synthesis of procoat protein in Escherichia coli infected with bacteriophage M13. Proc Natl Acad Sci U S A (1979) 1.99
Genetic mapping of the Escherichia coli leader (signal) peptidase gene (lep): a new approach for determining the map position of a cloned gene. J Bacteriol (1983) 1.98
Isolation of mutants in M13 coat protein that affect its synthesis, processing, and assembly into phage. J Biol Chem (1985) 1.96
Isolation of the Escherichia coli leader peptidase gene and effects of leader peptidase overproduction in vivo. Proc Natl Acad Sci U S A (1981) 1.96
The SecA and SecY subunits of translocase are the nearest neighbors of a translocating preprotein, shielding it from phospholipids. EMBO J (1993) 1.91
M13 procoat and a pre-immunoglobulin share processing specificity but use different membrane receptor mechanisms. Proc Natl Acad Sci U S A (1983) 1.90
Asymmetric orientation of a phage coat protein in cytoplasmic membrane of Escherichia coli. Proc Natl Acad Sci U S A (1975) 1.89
Molecular characterization of VAC1, a gene required for vacuole inheritance and vacuole protein sorting. J Biol Chem (1992) 1.89
Vam7p, a vacuolar SNAP-25 homolog, is required for SNARE complex integrity and vacuole docking and fusion. EMBO J (1998) 1.89
The "trigger factor cycle" includes ribosomes, presecretory proteins, and the plasma membrane. Cell (1988) 1.86
DNA polymerase 3 star requires ATP to start synthesis on a primed DNA. Proc Natl Acad Sci U S A (1973) 1.85
A holoenzyme form of deoxyribonucleic acid polymerase III. Isolation and properties. J Biol Chem (1974) 1.84
Energetics and intermediates of the assembly of Protein OmpA into the outer membrane of Escherichia coli. J Biol Chem (1983) 1.84
SecYEG and SecA are the stoichiometric components of preprotein translocase. J Biol Chem (1995) 1.81
Phosphatidylinositol 4,5-bisphosphate regulates two steps of homotypic vacuole fusion. Mol Biol Cell (2000) 1.80
Interactions of melittin, a preprotein model, with detergents. Biochemistry (1979) 1.80
The SecDFyajC domain of preprotein translocase controls preprotein movement by regulating SecA membrane cycling. EMBO J (1997) 1.74
Sequential action of two GTPases to promote vacuole docking and fusion. EMBO J (2000) 1.73
SecA protein needs both acidic phospholipids and SecY/E protein for functional high-affinity binding to the Escherichia coli plasma membrane. J Biol Chem (1991) 1.71
Synthesis of phage M13 coat protein and its assembly into membranes in vitro. Proc Natl Acad Sci U S A (1978) 1.69
Rho1p and Cdc42p act after Ypt7p to regulate vacuole docking. EMBO J (2001) 1.65
Ergosterol is required for the Sec18/ATP-dependent priming step of homotypic vacuole fusion. EMBO J (2001) 1.65
SecY, SecE, and band 1 form the membrane-embedded domain of Escherichia coli preprotein translocase. J Biol Chem (1992) 1.65
Thioredoxin is required for vacuole inheritance in Saccharomyces cerevisiae. J Cell Biol (1996) 1.64
Translational and post-translational cleavage of M13 procoat protein: extracts of both the cytoplasmic and outer membranes of Escherichia coli contain leader peptidase activity. Proc Natl Acad Sci U S A (1979) 1.62
A heterodimer of thioredoxin and I(B)2 cooperates with Sec18p (NSF) to promote yeast vacuole inheritance. J Cell Biol (1997) 1.60
LMA1 binds to vacuoles at Sec18p (NSF), transfers upon ATP hydrolysis to a t-SNARE (Vam3p) complex, and is released during fusion. Cell (1998) 1.59
Trigger factor depletion or overproduction causes defective cell division but does not block protein export. J Bacteriol (1990) 1.59
Genome-wide scan in a nationwide study sample of schizophrenia families in Finland reveals susceptibility loci on chromosomes 2q and 5q. Hum Mol Genet (2001) 1.57
Bacterial leader peptidase, a membrane protein without a leader peptide, uses the same export pathway as pre-secretory proteins. Cell (1984) 1.56
Determination of four biochemically distinct, sequential stages during vacuole inheritance in vitro. J Cell Biol (1994) 1.56
Specific recognition of the leader region of precursor proteins is required for the activation of translocation ATPase of Escherichia coli. Proc Natl Acad Sci U S A (1989) 1.53
The biosynthesis of membrane-bound M13 coat protein. Energetics and assembly intermediates. J Biol Chem (1982) 1.50
Biogenesis of the gram-negative bacterial envelope. Cell (1997) 1.49
The secY protein can act post-translationally to promote bacterial protein export. J Biol Chem (1986) 1.44
Vacuole acidification is required for trans-SNARE pairing, LMA1 release, and homotypic fusion. Proc Natl Acad Sci U S A (1999) 1.40
The cytoplasmic carboxy terminus of M13 procoat is required for the membrane insertion of its central domain. Nature (1986) 1.40
Melittin forms crystals which are suitable for high resolution X-ray structural analysis and which reveal a molecular 2-fold axis of symmetry. J Biol Chem (1980) 1.40
Leader peptidase is found in both the inner and outer membranes of Escherichia coli. J Biol Chem (1981) 1.37
Translocation can drive the unfolding of a preprotein domain. EMBO J (1993) 1.37
SecD and SecF are required for the proton electrochemical gradient stimulation of preprotein translocation. EMBO J (1994) 1.36
A new role for a SNARE protein as a regulator of the Ypt7/Rab-dependent stage of docking. Proc Natl Acad Sci U S A (2000) 1.35
The cytoplasmic domain of Escherichia coli leader peptidase is a "translocation poison" sequence. Proc Natl Acad Sci U S A (1988) 1.34
Proteins needed for vesicle budding from the Golgi complex are also required for the docking step of homotypic vacuole fusion. J Cell Biol (2000) 1.34
The PrlA and PrlG phenotypes are caused by a loosened association among the translocase SecYEG subunits. EMBO J (1999) 1.32
ProOmpA contains secondary and tertiary structure prior to translocation and is shielded from aggregation by association with SecB protein. EMBO J (1990) 1.31
M13 procoat inserts into liposomes in the absence of other membrane proteins. J Biol Chem (1985) 1.31
The role of the polar, carboxyl-terminal domain of Escherichia coli leader peptidase in its translocation across the plasma membrane. J Biol Chem (1986) 1.30
Membrane assembly from purified components. I. Isolated M13 procoat does not require ribosomes or soluble proteins for processing by membranes. Cell (1981) 1.21
Both an N-terminal 65-kDa domain and a C-terminal 30-kDa domain of SecA cycle into the membrane at SecYEG during translocation. Proc Natl Acad Sci U S A (1997) 1.20
Leader peptidase of Escherichia coli: critical role of a small domain in membrane assembly. Science (1987) 1.19
Membrane assembly from purified components. II. Assembly of M13 procoat into liposomes reconstituted with purified leader peptidase. Cell (1981) 1.17
Yeast vacuoles fragment when microtubules are disrupted. J Cell Biol (1988) 1.17
Sec-dependent membrane protein biogenesis: SecYEG, preprotein hydrophobicity and translocation kinetics control the stop-transfer function. EMBO J (1998) 1.14
Reconstitution of rapid and asymmetric assembly of M13 procoat protein into liposomes which have bacterial leader peptidase. J Biol Chem (1983) 1.14
The protease-protected 30 kDa domain of SecA is largely inaccessible to the membrane lipid phase. EMBO J (1997) 1.13
Requirements for substrate recognition by bacterial leader peptidase. EMBO J (1986) 1.13
Proton transfer is rate-limiting for translocation of precursor proteins by the Escherichia coli translocase. Proc Natl Acad Sci U S A (1991) 1.12