Published in Science on June 21, 1991
Differential amplification of rRNA genes by polymerase chain reaction. Appl Environ Microbiol (1992) 14.96
Bias in template-to-product ratios in multitemplate PCR. Appl Environ Microbiol (1998) 10.53
Consistent viral evolutionary changes associated with the progression of human immunodeficiency virus type 1 infection. J Virol (1999) 10.41
16S rRNA phylogenetic analysis of the bacterial endosymbionts associated with cytoplasmic incompatibility in insects. Proc Natl Acad Sci U S A (1992) 9.14
Inhibition and facilitation of nucleic acid amplification. Appl Environ Microbiol (1997) 9.00
Estimation of diversity and community structure through restriction fragment length polymorphism distribution analysis of bacterial 16S rRNA genes from a microbial mat at an active, hydrothermal vent system, Loihi Seamount, Hawaii. Appl Environ Microbiol (1994) 4.87
Evidence for immune selection of hepatitis C virus (HCV) putative envelope glycoprotein variants: potential role in chronic HCV infections. Proc Natl Acad Sci U S A (1992) 4.47
Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymerase system. Proc Natl Acad Sci U S A (1992) 4.04
Sequence identity of the n-1 product of a synthetic oligonucleotide. Nucleic Acids Res (1995) 3.88
Extension of base mispairs by Taq DNA polymerase: implications for single nucleotide discrimination in PCR. Nucleic Acids Res (1992) 3.74
Phylogenetic compositions of bacterioplankton from two California estuaries compared by denaturing gradient gel electrophoresis of 16S rDNA fragments. Appl Environ Microbiol (1996) 3.59
Theoretical uncertainty of measurements using quantitative polymerase chain reaction. Biophys J (1996) 3.30
Mycobacterium paratuberculosis DNA in Crohn's disease tissue. Gut (1992) 3.22
Multiplex detection of four pathogenic retroviruses using molecular beacons. Proc Natl Acad Sci U S A (1999) 3.10
Design and evaluation of Bacteroides DNA probes for the specific detection of human fecal pollution. Appl Environ Microbiol (1995) 3.08
Detection of adenoviruses and enteroviruses in polluted waters by nested PCR amplification. Appl Environ Microbiol (1994) 3.07
Concordant clonal delineation of methicillin-resistant Staphylococcus aureus by macrorestriction analysis and polymerase chain reaction genome fingerprinting. J Clin Microbiol (1993) 2.79
Towards practical, high-capacity, low-maintenance information storage in synthesized DNA. Nature (2013) 2.56
A cell-free protein synthesis system for high-throughput proteomics. Proc Natl Acad Sci U S A (2002) 2.55
A multiple-tubes approach for accurate genotyping of very small DNA samples by using PCR: statistical considerations. Am J Hum Genet (1992) 2.42
Single-nucleotide polymorphism identification assays using a thermostable DNA polymerase and delayed extraction MALDI-TOF mass spectrometry. Genome Res (1997) 2.37
The group A streptococcal virR49 gene controls expression of four structural vir regulon genes. Infect Immun (1995) 2.33
Comparison of plasmid- and chromosome-based polymerase chain reaction assays for detecting Chlamydia trachomatis nucleic acids. J Clin Microbiol (1993) 2.13
Extensive allelic variation in Cryptococcus neoformans. J Clin Microbiol (1992) 2.06
Typing of Legionella pneumophila strains by polymerase chain reaction-mediated DNA fingerprinting. J Clin Microbiol (1993) 2.04
An in silico approach for the evaluation of DNA barcodes. BMC Genomics (2010) 1.99
Advances in nucleic acid-based detection methods. Clin Microbiol Rev (1992) 1.84
PCR and single-strand conformational polymorphism for recognition of medically important opportunistic fungi. J Clin Microbiol (1995) 1.73
Direct and crossover PCR amplification to facilitate Tn5supF-based sequencing of lambda phage clones. Nucleic Acids Res (1991) 1.68
Development of species-specific DNA probes for Campylobacter jejuni, Campylobacter coli, and Campylobacter lari by polymerase chain reaction fingerprinting. J Clin Microbiol (1993) 1.67
Quantitative titration of nucleic acids by enzymatic amplification reactions run to saturation. Nucleic Acids Res (1993) 1.62
A PCR assay for identification of Enterococcus faecium. J Clin Microbiol (1997) 1.61
Genotyping Naegleria spp. and Naegleria fowleri isolates by interrepeat polymerase chain reaction. J Clin Microbiol (1992) 1.58
Genetic diversity of Borrelia burgdorferi sensu lato in ticks from mainland Portugal. J Clin Microbiol (2000) 1.50
Reinfection of liver graft by hepatitis C virus after liver transplantation. J Clin Invest (1992) 1.45
Identification of Bacteroides forsythus in subgingival plaque from patients with advanced periodontitis. J Clin Microbiol (1993) 1.45
The Nramp1 antimicrobial resistance gene segregates independently of resistance to virulent Mycobacterium tuberculosis. Immunology (1996) 1.44
Cystic fibrosis transmembrane conductance regulator (CFTR) gene mutations in allergic bronchopulmonary aspergillosis. Am J Hum Genet (1996) 1.38
Double-stranded DNA segments can efficiently prime the amplification of human genomic DNA. Nucleic Acids Res (1992) 1.37
RecOR suppression of recF mutant phenotypes in Escherichia coli K-12. J Bacteriol (1994) 1.28
Similarity between the group B and A streptococcal C5a peptidase genes. Infect Immun (1992) 1.27
Colorectal papillomavirus infection in patients with colorectal cancer. Clin Cancer Res (2005) 1.27
Rapid diagnosis of Mycobacterium tuberculosis bacteremia by PCR. J Clin Microbiol (1996) 1.24
Identification of Cryptosporidium spp. oocysts in United Kingdom noncarbonated natural mineral waters and drinking waters by using a modified nested PCR-restriction fragment length polymorphism assay. Appl Environ Microbiol (2003) 1.23
Detection of mycobacterial DNA in pleural fluid from patients with tuberculous pleurisy by means of the polymerase chain reaction: comparison of two protocols. Thorax (1992) 1.23
PCR in laboratory diagnosis of human Borrelia burgdorferi infections. Clin Microbiol Rev (1997) 1.23
Early expression of cytokine mRNA in mice infected with Listeria monocytogenes. Infect Immun (1992) 1.23
Advances in microfluidic PCR for point-of-care infectious disease diagnostics. Biotechnol Adv (2011) 1.22
Primary central nervous system lymphomas are derived from germinal-center B cells and show a preferential usage of the V4-34 gene segment. Am J Pathol (1999) 1.20
Analysis of genes encoding two unique type IIa immunoglobulin G-binding proteins expressed by a single group A streptococcal isolate. Infect Immun (1994) 1.20
Analysis of four diverse population groups indicates that a subset of cystic fibrosis mutations occur in common among Caucasians. Am J Hum Genet (1992) 1.17
Detection of genomic and intermediate replicative strands of hepatitis C virus in liver tissue by in situ hybridization. J Clin Invest (1993) 1.17
CD4 molecules with a diversity of mutations encompassing the CDR3 region efficiently support human immunodeficiency virus type 1 envelope glycoprotein-mediated cell fusion. J Virol (1993) 1.13
PCR amplification of highly GC-rich DNA template after denaturation by NaOH. Nucleic Acids Res (1993) 1.11
Human lupus anti-DNA autoantibodies undergo essentially primary V kappa gene rearrangements. EMBO J (1994) 1.10
Detection of Salmonella spp. in oysters by PCR. Appl Environ Microbiol (1994) 1.10
Comparison of polymerase chain reaction assay with culture for detection of genital mycoplasmas in perinatal infections. Eur J Clin Microbiol Infect Dis (1998) 1.09
Binding of Legionella pneumophila to macrophages increases cellular cytokine mRNA. Infect Immun (1994) 1.08
Increased expression of basement membrane collagen in human diabetic retinopathy. J Clin Invest (1994) 1.07
Increased clusterin expression in Fuchs' endothelial dystrophy. Invest Ophthalmol Vis Sci (2008) 1.04
Genetic diversity, population structure and linkage disequilibrium in elite Chinese winter wheat investigated with SSR markers. PLoS One (2012) 1.04
Competitive amplification and unspecific amplification in polymerase chain reaction with confronting two-pair primers. J Mol Diagn (2002) 1.03
Mycobacterium paratuberculosis DNA not detected in Crohn's disease tissue by fluorescent polymerase chain reaction. Gut (1995) 1.03
Obstacles of Multiplex Real-Time PCR for Bacterial 16S rDNA: Primer Specifity and DNA Decontamination of Taq Polymerase. Transfus Med Hemother (2010) 1.03
Length variation within intron 2 of the human IL-1 receptor antagonist protein gene (IL1RN). Nucleic Acids Res (1991) 1.02
Is 16S rDNA a reliable phylogenetic marker to characterize relationships below the family level in the enterobacteriaceae? J Mol Evol (2008) 1.01
Achalasia is not associated with measles or known herpes and human papilloma viruses. Dig Dis Sci (1997) 0.99
Molecular phylogenetic inference from saber-toothed cat fossils of Rancho La Brea. Proc Natl Acad Sci U S A (1992) 0.99
A rapid, non-radioactive screening test for fragile X mutations at the FRAXA and FRAXE loci. J Med Genet (1995) 0.95
Polymerase chain reaction in intraocular inflammation. Open Ophthalmol J (2008) 0.92
Rapid epidemiologic analysis of cytomegalovirus by using polymerase chain reaction amplification of the L-S junction region. J Clin Microbiol (1992) 0.92
Characterization of the immunoglobulin heavy chain complementarity determining region (CDR)-III sequences from human B cell precursor acute lymphoblastic leukemia cells. J Clin Invest (1992) 0.91
Interferon gamma quantification in cerebrospinal fluid compared with PCR for the diagnosis of tuberculous meningitis. J Neurol (2006) 0.89
Protein tyrosine phosphatase, PTP1B, expression and activity in rat corneal endothelial cells. Mol Vis (2007) 0.88
Polymerase chain reaction: basic protocol plus troubleshooting and optimization strategies. J Vis Exp (2012) 0.87
Loss of allosteric control but retention of the bifunctional catalytic competence of a fusion protein formed by excision of 260 base pairs from the 3' terminus of pheA from Erwinia herbicola. Appl Environ Microbiol (1992) 0.87
Optochemical control of deoxyoligonucleotide function via a nucleobase-caging approach. Acc Chem Res (2013) 0.87
Sequencing heavy- and light-chain variable genes of single B-hybridoma cells by total enzymatic amplification. Proc Natl Acad Sci U S A (1992) 0.87
Vertical distribution and diversity of bacteria and archaea in sulfide and methane-rich cold seep sediments located at the base of the Florida Escarpment. Extremophiles (2006) 0.86
Polymerase chain reaction in the diagnosis of uveitis. Int Ophthalmol Clin (2005) 0.86
Fast and easy method for construction of plasmid vectors using modified quick-change mutagenesis. Methods Mol Biol (2012) 0.85
Rapid, sensitive PCR-based detection of mycoplasmas in simulated samples of animal sera. Appl Environ Microbiol (1994) 0.84
Development of a nested PCR assay for detection of feline infectious peritonitis virus in clinical specimens. J Clin Microbiol (1997) 0.83
Identification of a cytochrome P450 gene by reverse transcription--PCR using degenerate primers containing inosine. Proc Natl Acad Sci U S A (1993) 0.83
Correlation between extent of osteolytic damage and metastatic burden of human breast cancer metastasis in nude mice: real-time PCR quantitation. Clin Exp Metastasis (2002) 0.83
Extensive recombination due to heteroduplexes generates large amounts of artificial gene fragments during PCR. PLoS One (2014) 0.82
Peptide rescue of an N-terminal truncation of the Stoffel fragment of taq DNA polymerase. Protein Sci (1996) 0.81
Structural heterogeneity of the streptococcal C5a peptidase gene in Streptococcus pyogenes. J Bacteriol (2002) 0.81
Approaches to analyse dynamic microbial communities such as those seen in cystic fibrosis lung. Hum Genomics (2009) 0.80
Interrogating the transgenic genome: development of an interspecies tiling array. Syst Biol Reprod Med (2011) 0.78
Molecular detection of Sarcocystis species in slaughtered sheep by PCR-RFLP from south-western of Iran. J Parasit Dis (2013) 0.78
Detection of carcinoembryonic antigen mRNA in the mesenteric vein of patients with resectable colorectal cancer. Surg Today (1998) 0.76
Field effect sensors for nucleic Acid detection: recent advances and future perspectives. Sensors (Basel) (2015) 0.76
The genome of the THE I human transposable repetitive elements is composed of a basic motif homologous to an ancestral immunoglobulin gene sequence. Proc Natl Acad Sci U S A (1994) 0.75
Prevention of product carry-over by single tube two-round (ST-2R) PCR: application to BCR-ABL analysis in chronic myelogenous leukemia. Nucleic Acids Res (1995) 0.75
Establishment of conditions for the detection of bovine herpesvirus-1 by polymerase chain reaction using primers in the thymidine kinase region. Can J Vet Res (1995) 0.75
DNA enzyme immunoassay of the PCR-amplified HLA-DQ alpha gene for estimating residual leukocytes in filtered blood. Clin Diagn Lab Immunol (1995) 0.75
The polymerase chain reaction and other amplification techniques in immunological research and diagnosis. Immunology (1992) 0.75
Human papillomaviruses in hypopharyngeal squamous cell carcinoma. Indian J Otolaryngol Head Neck Surg (1998) 0.75
DNA amplification for direct detection of HIV-1 in DNA of peripheral blood mononuclear cells. Science (1988) 13.46
Effects of primer-template mismatches on the polymerase chain reaction: human immunodeficiency virus type 1 model studies. Nucleic Acids Res (1990) 9.28
Identification of human immunodeficiency virus sequences by using in vitro enzymatic amplification and oligomer cleavage detection. J Virol (1987) 5.27
Human immunodeficiency virus type 1 infection in homosexual men who remain seronegative for prolonged periods. N Engl J Med (1989) 4.20
Three cdg operons control cellular turnover of cyclic di-GMP in Acetobacter xylinum: genetic organization and occurrence of conserved domains in isoenzymes. J Bacteriol (1998) 3.81
Cloning and endonuclease mapping of the hepatitis B viral genome. Nature (1979) 3.62
Enzymatic amplification of HTLV-I viral sequences from peripheral blood mononuclear cells and infected tissues. Blood (1988) 2.98
Enzymatic gene amplification: qualitative and quantitative methods for detecting proviral DNA amplified in vitro. J Infect Dis (1988) 2.90
Human immunodeficiency virus type 1 (HIV-1) infection a median of 18 months before a diagnostic western blot. Evidence from a cohort of homosexual men. Ann Intern Med (1989) 2.44
Detection of hepatitis B virus sequences in serum by using in vitro enzymatic amplification. J Virol Methods (1988) 2.36
Detection of ras gene mutations in pancreatic juice and peripheral blood of patients with pancreatic adenocarcinoma. Cancer Res (1993) 2.22
Loss of human immunodeficiency virus type 1 (HIV-1) antibodies with evidence of viral infection in asymptomatic homosexual men. A report from the Multicenter AIDS Cohort Study. Ann Intern Med (1988) 2.18
The use of terminal blocking groups for the specific joining of oligonucleotides in RNA ligase reactions containing equimolar concentrations of acceptor and donor molecules. Nucleic Acids Res (1976) 2.03
Characterization of a pre-S polypeptide on the surfaces of infectious avian hepadnavirus particles. J Virol (1987) 1.95
Human immunodeficiency virus type 1 detected in all seropositive symptomatic and asymptomatic individuals. J Clin Microbiol (1990) 1.88
A sensitive method for the identification of uncharacterized viruses related to known virus groups: hepadnavirus model system. Proc Natl Acad Sci U S A (1988) 1.76
Characterization of a sequence of human T cell leukemia virus type I from a patient with chronic progressive myelopathy. J Infect Dis (1988) 1.71
High prevalence of HTLV-II among intravenous drug abusers: PCR confirmation and typing. AIDS Res Hum Retroviruses (1990) 1.64
Quantitation of HIV-1 proviral DNA relative to cellular DNA by the polymerase chain reaction. Anal Biochem (1990) 1.57
A guide to the design and use of mismatched and degenerate primers. PCR Methods Appl (1994) 1.38
Absence of HIV-1 infection in antibody-negative sexual partners of HIV-1 infected hemophiliacs. Transfusion (1989) 1.36
Hepatitis B virus polypeptide X: expression in Escherichia coli and identification of specific antibodies in sera from hepatitis B virus-infected humans. J Virol (1986) 1.35
Outcome of African American men screened for prostate cancer: the Detroit Education and Early Detection Study. J Urol (1997) 1.24
Identification of hepatitis B virus polypeptides encoded by the entire pre-s open reading frame. J Virol (1985) 1.23
Identification and localization of pre-s-encoded polypeptides from woodchuck and ground squirrel hepatitis viruses. J Virol (1986) 1.21
Determination of a unique and immunodominant epitope of human T cell lymphotropic virus type I. J Infect Dis (1990) 1.15
Enzymatic amplification of the human immunodeficiency virus in peripheral blood mononuclear cells from pediatric patients. J Infect Dis (1989) 1.12
A polyclonal CD4+ and CD8+ lymphocytosis in a patient doubly infected with HTLV-I and HIV-1: a clinical and molecular analysis. Am J Hematol (1989) 1.11
Amplification of ssb-1 mutant single-stranded DNA-binding protein in Escherichia coli. J Mol Biol (1983) 1.08
Positive selection in the human genome inferred from human-chimp-mouse orthologous gene alignments. Cold Spring Harb Symp Quant Biol (2003) 1.07
The eight genes of bacteriophages phi X174 and S13 and comparison of the phage-specified proteins. J Mol Biol (1970) 1.00
Low incidence of HTLV infections in random blood donors with indeterminate western blot patterns. Transfusion (1990) 0.99
'Single addition' and 'transnucleotidation' reactions catalyzed by polynucleotide phosphorylase. Effect of enzymatic removal of inorganic phosphate during reaction. Nucleic Acids Res (1974) 0.96
Modified Western blot assay for confirmation and differentiation of human T cell lymphotropic virus types I and II. J Infect Dis (1991) 0.95
Pneumothorax complicating enteral feeding tube placement. JPEN J Parenter Enteral Nutr (1985) 0.95
Serological characterization and gene localization of an Escherichia coli-expressed 37-kilodalton Treponema pallidum antigen. Infect Immun (1986) 0.93
Predicted secondary structure similarity in the absence of primary amino acid sequence homology: hepatitis B virus open reading frames. Proc Natl Acad Sci U S A (1984) 0.93
Detection and characterization of HIV-1 by polymerase chain reaction. J Acquir Immune Defic Syndr (1990) 0.90
Hepatitis B virus particles contain a polypeptide encoded by the largest open reading frame: a putative reverse transcriptase. J Virol (1988) 0.90
Binding of glucocorticoid receptors to DNA. J Biol Chem (1975) 0.90
The 140-kDa adenovirus DNA polymerase is recognized by antibodies to Escherichia coli-synthesized determinants predicted from an open reading frame on the adenovirus genome. Proc Natl Acad Sci U S A (1985) 0.88
Significance of human T-lymphotropic virus type I indeterminant serological findings among healthy individuals. Vox Sang (1991) 0.85
Real-time dynamics of string breaking. Phys Rev Lett (2013) 0.83
Amplification and analysis of specific DNA and RNA sequences of bovine leukemia virus from infected cows by polymerase chain reaction. J Clin Microbiol (1992) 0.83
Activity of polynucleotide phosphorylase with nucleoside dephosphates containing sugar ring modifications. Biochemistry (1978) 0.83
Transmission of serologically silent hepatitis B virus along with hepatitis C virus in two cases of posttransfusion hepatitis. Transfusion (1992) 0.82
Selective amplification of RNA utilizing the nucleotide analog dITP and Thermus thermophilus DNA polymerase. Nucleic Acids Res (1996) 0.80
Evaluation of HIV type 1 western blot-indeterminate blood donors for the presence of human or bovine retroviruses. AIDS Res Hum Retroviruses (1995) 0.80
Current status of minimally invasive esophagectomy. Minerva Chir (2004) 0.78
Laparoscopic esophagectomy. Minerva Chir (2005) 0.75
The effect of parabiosis on audiogenic convulsions in seizure-susceptible mice. Exp Neurol (1970) 0.75
New rulings on the Cetus patent for taq polymerase. Mol Diagn (2000) 0.75
Diagnosis of coronary heart disease. J Rehabil (1966) 0.75
Polymerase chain reaction for seronegative health care workers with parenteral exposure to HIV-infected patients. N Engl J Med (1989) 0.75