Published in Proc Natl Acad Sci U S A on September 21, 2006
Biological phosphoryl-transfer reactions: understanding mechanism and catalysis. Annu Rev Biochem (2011) 1.94
A comparison of vanadate to a 2'-5' linkage at the active site of a small ribozyme suggests a role for water in transition-state stabilization. RNA (2007) 1.27
Mechanistic evidence for a front-side, SNi-type reaction in a retaining glycosyltransferase. Nat Chem Biol (2011) 1.24
Briefly bound to activate: transient binding of a second catalytic magnesium activates the structure and dynamics of CDK2 kinase for catalysis. Structure (2011) 1.21
The catalytic scaffold of the haloalkanoic acid dehalogenase enzyme superfamily acts as a mold for the trigonal bipyramidal transition state. Proc Natl Acad Sci U S A (2008) 1.19
Insights into the reaction of protein-tyrosine phosphatase 1B: crystal structures for transition state analogs of both catalytic steps. J Biol Chem (2010) 1.10
Structure-function analysis of the 3' phosphatase component of T4 polynucleotide kinase/phosphatase. Virology (2007) 1.05
Kinetic isotope effects for alkaline phosphatase reactions: implications for the role of active-site metal ions in catalysis. J Am Chem Soc (2007) 1.03
Atomic details of near-transition state conformers for enzyme phosphoryl transfer revealed by MgF-3 rather than by phosphoranes. Proc Natl Acad Sci U S A (2010) 0.96
Near attack conformers dominate β-phosphoglucomutase complexes where geometry and charge distribution reflect those of substrate. Proc Natl Acad Sci U S A (2012) 0.86
Catalytic mechanism of α-phosphate attack in dUTPase is revealed by X-ray crystallographic snapshots of distinct intermediates, 31P-NMR spectroscopy and reaction path modelling. Nucleic Acids Res (2013) 0.80
α-Fluorophosphonates reveal how a phosphomutase conserves transition state conformation over hexose recognition in its two-step reaction. Proc Natl Acad Sci U S A (2014) 0.80
(19) F NMR and DFT Analysis Reveal Structural and Electronic Transition State Features for RhoA-Catalyzed GTP Hydrolysis. Angew Chem Int Ed Engl (2016) 0.79
Promiscuity in the Enzymatic Catalysis of Phosphate and Sulfate Transfer. Biochemistry (2016) 0.77
Metal Fluorides: Tools for Structural and Computational Analysis of Phosphoryl Transfer Enzymes. Top Curr Chem (J) (2017) 0.75
Crystallography & NMR system: A new software suite for macromolecular structure determination. Acta Crystallogr D Biol Crystallogr (1998) 169.28
A perspective on enzyme catalysis. Science (2003) 5.15
The pentacovalent phosphorus intermediate of a phosphoryl transfer reaction. Science (2003) 3.41
Phosphotransfer networks and cellular energetics. J Exp Biol (2003) 2.79
The rate of hydrolysis of phosphomonoester dianions and the exceptional catalytic proficiencies of protein and inositol phosphatases. Proc Natl Acad Sci U S A (2003) 2.76
The structure of bovine F1-ATPase inhibited by ADP and beryllium fluoride. EMBO J (2004) 2.08
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BeF(3)(-) acts as a phosphate analog in proteins phosphorylated on aspartate: structure of a BeF(3)(-) complex with phosphoserine phosphatase. Proc Natl Acad Sci U S A (2001) 1.62
Enzymatic transition states and transition state analog design. Annu Rev Biochem (1998) 1.48
Caught in the act: the structure of phosphorylated beta-phosphoglucomutase from Lactococcus lactis. Biochemistry (2002) 1.43
Purification and characterization of two phosphoglucomutases from Lactococcus lactis subsp. lactis and their regulation in maltose- and glucose-utilizing cells. J Bacteriol (1994) 1.38
Enzymatic transition states and transition state analogues. Curr Opin Struct Biol (2005) 1.29
MgF(3)(-) as a transition state analog of phosphoryl transfer. Chem Biol (2002) 1.25
Analysis of the substrate specificity loop of the HAD superfamily cap domain. Biochemistry (2004) 1.20
The biochemistry and physiology of metallic fluoride: action, mechanism, and implications. Crit Rev Oral Biol Med (2003) 1.17
AlF3 mimics the transition state of protein phosphorylation in the crystal structure of nucleoside diphosphate kinase and MgADP. Proc Natl Acad Sci U S A (1997) 1.15
Thermodynamic and extrathermodynamic requirements of enzyme catalysis. Biophys Chem (2003) 1.13
Product formation and phosphoglucomutase activities in Lactococcus lactis: cloning and characterization of a novel phosphoglucomutase gene. Microbiology (1997) 1.11
Chemical confirmation of a pentavalent phosphorane in complex with beta-phosphoglucomutase. J Am Chem Soc (2005) 1.05
Catalytic cycling in beta-phosphoglucomutase: a kinetic and structural analysis. Biochemistry (2005) 1.05
Effects of domain dissection on the folding and stability of the 43 kDa protein PGK probed by NMR. J Mol Biol (2003) 1.03
pH influences fluoride coordination number of the AlFx phosphoryl transfer transition state analog. Nat Struct Biol (1999) 0.95
High-energy intermediate or stable transition state analogue: theoretical perspective of the active site and mechanism of beta-phosphoglucomutase. J Am Chem Soc (2004) 0.93
Comment on "The pentacovalent phosphorus intermediate of a phosphoryl transfer reaction". Science (2003) 0.93
Inhibition of the ATPase activity of Escherichia coli ATP synthase by magnesium fluoride. FEBS Lett (2005) 0.90
Fluoride inhibition of yeast enolase: crystal structure of the enolase-Mg(2+)-F(-)-Pi complex at 2.6 A resolution. Proteins (1993) 0.88
Purification and enzymatic characterization of PgcM: a beta-phosphoglucomutase and glucose-1-phosphate phosphodismutase of Bacillus subtilis. Arch Microbiol (2000) 0.85
Crystallization and preliminary X-ray diffraction studies of beta-phosphoglucomutase from Lactococcus lactus. Acta Crystallogr D Biol Crystallogr (2002) 0.84
Metabolism of trehalose in Euglena gracilis. Beta glucose 1,6-bisphosphate, activation factor of phosphoglucomutase for beta glucose 1-phosphate. Eur J Biochem (1974) 0.83
Chemistry. Seeing is believing. Science (2003) 0.83
Computational study of a transition state analog of phosphoryl transfer in the Ras-RasGAP complex: AlF(x) versus MgF3-. J Mol Model (2005) 0.81
Formation constants beta(2) of calcium and magnesium fluorides at 25 degrees C. Talanta (2000) 0.78
The structure of H5N1 avian influenza neuraminidase suggests new opportunities for drug design. Nature (2006) 6.00
Structure and catalytic mechanism of the human histone methyltransferase SET7/9. Nature (2003) 4.00
The rate of hydrolysis of phosphomonoester dianions and the exceptional catalytic proficiencies of protein and inositol phosphatases. Proc Natl Acad Sci U S A (2003) 2.76
Microdroplets in microfluidics: an evolving platform for discoveries in chemistry and biology. Angew Chem Int Ed Engl (2010) 2.61
Microdroplets: a sea of applications? Lab Chip (2008) 2.55
Crystal structure and functional analysis of the histone methyltransferase SET7/9. Cell (2002) 2.27
Picoliter cell lysate assays in microfluidic droplet compartments for directed enzyme evolution. Chem Biol (2012) 1.87
Cross-talk between histone modifications in response to histone deacetylase inhibitors: MLL4 links histone H3 acetylation and histone H3K4 methylation. J Biol Chem (2006) 1.82
The time required for water attack at the phosphorus atom of simple phosphodiesters and of DNA. Proc Natl Acad Sci U S A (2006) 1.78
Static microdroplet arrays: a microfluidic device for droplet trapping, incubation and release for enzymatic and cell-based assays. Lab Chip (2008) 1.59
Catalytic roles for carbon-oxygen hydrogen bonding in SET domain lysine methyltransferases. J Biol Chem (2006) 1.53
Directed evolution of a gatekeeper domain in nonribosomal peptide synthesis. Chem Biol (2011) 1.45
Time-resolved NMR studies of RNA folding. Biopolymers (2007) 1.40
Antibiotic recognition by binuclear metallo-beta-lactamases revealed by X-ray crystallography. J Am Chem Soc (2005) 1.30
Development of quantitative cell-based enzyme assays in microdroplets. Anal Chem (2008) 1.29
Structural and functional analysis of RNA and TAP binding to SF2/ASF. EMBO Rep (2007) 1.28
Essential role of proline isomerization in stefin B tetramer formation. J Mol Biol (2006) 1.23
The crystal structure of diadenosine tetraphosphate hydrolase from Caenorhabditis elegans in free and binary complex forms. Structure (2002) 1.21
Obligate heterodimerization of the archaeal Alba2 protein with Alba1 provides a mechanism for control of DNA packaging. Structure (2005) 1.20
Continuous-flow polymerase chain reaction of single-copy DNA in microfluidic microdroplets. Anal Chem (2009) 1.19
What makes an enzyme promiscuous? Curr Opin Chem Biol (2010) 1.17
Three metal ions participate in the reaction catalyzed by T5 flap endonuclease. J Biol Chem (2008) 1.16
Determination of reference genes for circadian studies in different tissues and mouse strains. BMC Mol Biol (2010) 1.16
Determinants of the endosomal localization of sorting nexin 1. Mol Biol Cell (2005) 1.15
The residue 129 polymorphism in human prion protein does not confer susceptibility to Creutzfeldt-Jakob disease by altering the structure or global stability of PrPC. J Biol Chem (2004) 1.14
Use of relaxation enhancements in a paramagnetic environment for the structure determination of proteins using NMR spectroscopy. Angew Chem Int Ed Engl (2009) 1.14
Controlling the retention of small molecules in emulsion microdroplets for use in cell-based assays. Anal Chem (2009) 1.13
ATP analogues with modified phosphate chains and their selectivity for rat P2X2 and P2X2/3 receptors. Br J Pharmacol (2003) 1.12
Simultaneous determination of gene expression and enzymatic activity in individual bacterial cells in microdroplet compartments. J Am Chem Soc (2009) 1.11
Transition state analogue structures of human phosphoglycerate kinase establish the importance of charge balance in catalysis. J Am Chem Soc (2010) 1.09
The potential of microfluidic water-in-oil droplets in experimental biology. Mol Biosyst (2009) 1.08
An efficient, multiply promiscuous hydrolase in the alkaline phosphatase superfamily. Proc Natl Acad Sci U S A (2010) 1.07
Turnover-based in vitro selection and evolution of biocatalysts from a fully synthetic antibody library. Nat Biotechnol (2003) 1.06
A non-uniformly sampled 4D HCC(CO)NH-TOCSY experiment processed using maximum entropy for rapid protein sidechain assignment. J Magn Reson (2010) 1.06
An integrated device for monitoring time-dependent in vitro expression from single genes in picolitre droplets. Chembiochem (2008) 1.06
Solution structure of the helicase-interaction domain of the primase DnaG: a model for helicase activation. Structure (2005) 1.04
Combined gas chromatographic/mass spectrometric analysis of cholesterol precursors and plant sterols in cultured cells. J Chromatogr B Analyt Technol Biomed Life Sci (2009) 1.04
Prospectively versus retrospectively ECG-gated 256-slice coronary CT angiography: image quality and radiation dose over expanded heart rates. Int J Cardiovasc Imaging (2010) 1.04
A double droplet trap system for studying mass transport across a droplet-droplet interface. Lab Chip (2010) 1.04
Effects of domain dissection on the folding and stability of the 43 kDa protein PGK probed by NMR. J Mol Biol (2003) 1.03
Microfluidic droplets: new integrated workflows for biological experiments. Curr Opin Chem Biol (2010) 1.03
An altered mechanism of hydrolysis for a metal-complexed phosphate diester. J Am Chem Soc (2002) 1.01
Cystatin forms a tetramer through structural rearrangement of domain-swapped dimers prior to amyloidogenesis. J Mol Biol (2004) 1.00
Fast protein motions are coupled to enzyme H-transfer reactions. J Am Chem Soc (2013) 1.00
Structure and dynamics of coxsackievirus B4 2A proteinase, an enyzme involved in the etiology of heart disease. J Virol (2006) 1.00
Definable equilibrium states in the folding of human prion protein. Biochemistry (2005) 0.99
Biochemical, crystallographic, and mutagenic characterization of hint, the AMP-lysine hydrolase, with novel substrates and inhibitors. J Biol Chem (2004) 0.99
Ultrarapid generation of femtoliter microfluidic droplets for single-molecule-counting immunoassays. ACS Nano (2013) 0.99
Simultaneously enhancing spectral resolution and sensitivity in heteronuclear correlation NMR spectroscopy. Angew Chem Int Ed Engl (2013) 0.99
New genotype-phenotype linkages for directed evolution of functional proteins. Curr Opin Struct Biol (2005) 0.98
One in a million: flow cytometric sorting of single cell-lysate assays in monodisperse picolitre double emulsion droplets for directed evolution. Anal Chem (2014) 0.98
The human histone acetyltransferase P/CAF is a promiscuous histone propionyltransferase. Chembiochem (2008) 0.98
An efficient method to assemble linear DNA templates for in vitro screening and selection systems. Nucleic Acids Res (2009) 0.98
Multiple forms of copper (II) co-ordination occur throughout the disordered N-terminal region of the prion protein at pH 7.4. Biochem J (2006) 0.98
Efficient catalytic promiscuity in an enzyme superfamily: an arylsulfatase shows a rate acceleration of 10(13) for phosphate monoester hydrolysis. J Am Chem Soc (2008) 0.98
Anionic charge is prioritized over geometry in aluminum and magnesium fluoride transition state analogs of phosphoryl transfer enzymes. J Am Chem Soc (2008) 0.97
An integrated cell culture lab on a chip: modular microdevices for cultivation of mammalian cells and delivery into microfluidic microdroplets. Lab Chip (2009) 0.97
A highly reactive mononuclear Zn(II) complex for phosphodiester cleavage. J Am Chem Soc (2005) 0.97
Brønsted analysis and rate-limiting steps for the T5 flap endonuclease catalyzed hydrolysis of exonucleolytic substrates. Biochemistry (2010) 0.97
Experimental access to HSQC spectra decoupled in all frequency dimensions. J Magn Reson (2009) 0.96
The Fantastic Four: A plug 'n' play set of optimal control pulses for enhancing NMR spectroscopy. J Magn Reson (2012) 0.96
Atomic details of near-transition state conformers for enzyme phosphoryl transfer revealed by MgF-3 rather than by phosphoranes. Proc Natl Acad Sci U S A (2010) 0.96
Efficient catalytic promiscuity for chemically distinct reactions. Angew Chem Int Ed Engl (2009) 0.95
Structure and mechanism of imidazoleglycerol-phosphate dehydratase. Structure (2005) 0.94
Folding kinetics of the human prion protein probed by temperature jump. Proc Natl Acad Sci U S A (2009) 0.94
Synthesis of 5-fluoropyrimidine nucleotides as sensitive NMR probes of RNA structure. J Am Chem Soc (2007) 0.94
Reprogramming of Escherichia coli K-12 metabolism during the initial phase of transition from an anaerobic to a micro-aerobic environment. PLoS One (2011) 0.94
New NMR experiments for RNA nucleobase resonance assignment and chemical shift analysis of an RNA UUCG tetraloop. J Biomol NMR (2004) 0.94
Dineopentyl phosphate hydrolysis: evidence for stepwise water attack. J Org Chem (2008) 0.93
Altered mechanisms of reactions of phosphate esters bridging a dinuclear metal center. J Am Chem Soc (2004) 0.93
Comment on "The pentacovalent phosphorus intermediate of a phosphoryl transfer reaction". Science (2003) 0.93
Mechanistic study of protein phosphatase-1 (PP1), a catalytically promiscuous enzyme. J Am Chem Soc (2008) 0.93
Cryogenic and laser photoexcitation studies identify multiple roles for active site residues in the light-driven enzyme protochlorophyllide oxidoreductase. J Biol Chem (2009) 0.92
Removal of background signals from fluorescence thermometry measurements in PDMS microchannels using fluorescence lifetime imaging. Lab Chip (2009) 0.92
Amino-functionalized DNA: the properties of C5-amino-alkyl substituted 2'-deoxyuridines and their application in DNA triplex formation. Nucleic Acids Res (2005) 0.92