Published in Nucleic Acids Res on July 15, 2003
Tagging a T. brucei RRNA locus improves stable transfection efficiency and circumvents inducible expression position effects. Mol Biochem Parasitol (2005) 1.89
Trypanosoma brucei homologous recombination is dependent on substrate length and homology, though displays a differential dependence on mismatch repair as substrate length decreases. Nucleic Acids Res (2007) 1.29
The small chromosomes of Trypanosoma brucei involved in antigenic variation are constructed around repetitive palindromes. Genome Res (2004) 1.20
A novel role for BRCA1 in regulating breast cancer cell spreading and motility. J Cell Biol (2011) 1.08
Protein targeting of an unusual, evolutionarily conserved adenylate kinase to a eukaryotic flagellum. Mol Biol Cell (2004) 1.03
RNA polymerase I transcription stimulates homologous recombination in Trypanosoma brucei. Mol Biochem Parasitol (2007) 0.94
TbISWI regulates multiple polymerase I (Pol I)-transcribed loci and is present at Pol II transcription boundaries in Trypanosoma brucei. Eukaryot Cell (2011) 0.88
Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-selectable genes. Nature (1988) 20.36
Targeting, disruption, replacement, and allele rescue: integrative DNA transformation in yeast. Methods Enzymol (1991) 16.88
A tightly regulated inducible expression system for conditional gene knock-outs and dominant-negative genetics in Trypanosoma brucei. Mol Biochem Parasitol (1999) 11.77
Cultivation and in vitro cloning or procyclic culture forms of Trypanosoma brucei in a semi-defined medium. Short communication. Acta Trop (1979) 8.43
Homologous recombination in Escherichia coli: dependence on substrate length and homology. Genetics (1986) 7.97
High frequency targeting of genes to specific sites in the mammalian genome. Cell (1986) 6.91
Inactivation of the mouse Msh2 gene results in mismatch repair deficiency, methylation tolerance, hyperrecombination, and predisposition to cancer. Cell (1995) 5.54
Highly efficient gene targeting in embryonic stem cells through homologous recombination with isogenic DNA constructs. Proc Natl Acad Sci U S A (1992) 4.50
Reexamination of gene targeting frequency as a function of the extent of homology between the targeting vector and the target locus. Mol Cell Biol (1992) 4.19
Homologous integration in mammalian cells without target gene selection. Genes Dev (1988) 3.54
Tubulin genes are tandemly linked and clustered in the genome of trypanosoma brucei. Cell (1983) 3.40
Three small RNAs within the 10 kb trypanosome rRNA transcription unit are analogous to domain VII of other eukaryotic 28S rRNAs. Nucleic Acids Res (1986) 3.30
Gene replacement in parasitic protozoa. Nature (1990) 3.15
Targeted insertion of the neomycin phosphotransferase gene into the tubulin gene cluster of Trypanosoma brucei. Nature (1990) 2.91
Substrate length requirements for efficient mitotic recombination in Saccharomyces cerevisiae. Mol Cell Biol (1993) 2.81
Manipulating the mammalian genome by homologous recombination. Proc Natl Acad Sci U S A (2001) 2.71
Mismatch repair, genetic stability, and cancer. Science (1994) 2.65
Gene targeting in normal and amplified cell lines. Nature (1990) 2.54
Expression of a bacterial gene in a trypanosomatid protozoan. Science (1989) 2.52
Inducible gene expression in trypanosomes mediated by a prokaryotic repressor. Science (1995) 2.42
Stable expression of the bacterial neor gene in Leishmania enriettii. Nature (1990) 2.25
Vectors for inducible expression of toxic gene products in bloodstream and procyclic Trypanosoma brucei. Mol Biochem Parasitol (1997) 2.17
Homologous recombination and stable transfection in the parasitic protozoan Trypanosoma brucei. Science (1990) 2.06
Stable integrative transformation of Trypanosoma brucei that occurs exclusively by homologous recombination. Proc Natl Acad Sci U S A (1991) 1.75
Regulated processive transcription of chromatin by T7 RNA polymerase in Trypanosoma brucei. Nucleic Acids Res (1998) 1.70
Tubulin genes of Trypanosoma brucei: a tightly clustered family of alternating genes. Proc Natl Acad Sci U S A (1983) 1.61
Positive genetic selection for gene disruption in mammalian cells by homologous recombination. Proc Natl Acad Sci U S A (1989) 1.50
Axenic culture of African trypanosome bloodstream forms. Parasitol Today (1994) 1.47
Expression and function of surface proteins in Trypanosoma brucei. Mol Biochem Parasitol (1998) 1.39
In vivo epitope tagging of Trypanosoma brucei genes using a one step PCR-based strategy. Mol Biochem Parasitol (2001) 1.37
Predominance of duplicative VSG gene conversion in antigenic variation in African trypanosomes. Mol Cell Biol (1999) 1.34
Characterization of satellite DNA in Trypanosoma brucei and Trypanosoma cruzi. J Mol Biol (1983) 1.30
The frequency of gene targeting in yeast depends on the number of target copies. Proc Natl Acad Sci U S A (1994) 1.24
Chromosome structure: DNA nucleotide sequence elements of a subset of the minichromosomes of the protozoan Trypanosoma brucei. Mol Cell Biol (1991) 1.18
Tandemly repeated DNA is a target for the partial replacement of thymine by beta-D-glucosyl-hydroxymethyluracil in Trypanosoma brucei. Mol Biochem Parasitol (2000) 1.18
Homologous recombination of SV40 DNA in COS7 cells occurs with high frequency in a gene dose independent fashion. Nucleic Acids Res (1988) 1.17
Autonomously replicating single-copy episomes in Trypanosoma brucei show unusual stability. EMBO J (1993) 1.16
Parameters controlling the rate of gene targeting frequency in the protozoan parasite Leishmania. Nucleic Acids Res (1997) 1.09
Polymerase chain reaction-based gene disruption in Trypanosoma brucei. Mol Biochem Parasitol (1997) 1.08
Stable transformation of Trypanosoma cruzi: inactivation of the PUB12.5 polyubiquitin gene by targeted gene disruption. Mol Biochem Parasitol (1993) 1.06
The African trypanosome genome. Int J Parasitol (2000) 1.02
Antigenic variation in trypanosomes. Arch Med Res (1996) 1.01
A ribosomal RNA gene promoter at the telomere of a mini-chromosome in Trypanosoma brucei. Nucleic Acids Res (1992) 0.99
Targeting of exogenous DNA into Trypanosoma brucei requires a high degree of homology between donor and target DNA. Mol Biochem Parasitol (1997) 0.98
Mitochondrial minicircle DNA supports plasmid replication and maintenance in nuclei of Trypanosoma brucei. Proc Natl Acad Sci U S A (1994) 0.93
Diversity and dynamics of the minichromosomal karyotype in Trypanosoma brucei. Mol Biochem Parasitol (2001) 0.89
Trypanosoma brucei brucei: differences in the nuclear chromatin of bloodstream forms and procyclic culture forms. Parasitology (1993) 0.88
Direct visualisation of individual gene organisation in Trypanosoma brucei by high-resolution in situ hybridisation. Chromosoma (1998) 0.84
The genome of the African trypanosome Trypanosoma brucei. Science (2005) 11.48
The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease. Science (2005) 7.61
Flagellar motility is required for the viability of the bloodstream trypanosome. Nature (2006) 4.71
The Meckel-Gruber Syndrome proteins MKS1 and meckelin interact and are required for primary cilium formation. Hum Mol Genet (2006) 3.06
Targeting of a tetracycline-inducible expression system to the transcriptionally silent minichromosomes of Trypanosoma brucei. Mol Biochem Parasitol (2002) 2.74
Centriole/basal body morphogenesis and migration during ciliogenesis in animal cells. J Cell Sci (2007) 2.46
A high-order trans-membrane structural linkage is responsible for mitochondrial genome positioning and segregation by flagellar basal bodies in trypanosomes. Mol Biol Cell (2003) 2.09
Functional genomics in Trypanosoma brucei: a collection of vectors for the expression of tagged proteins from endogenous and ectopic gene loci. Mol Biochem Parasitol (2007) 1.96
Reconstructing the evolutionary history of the centriole from protein components. J Cell Sci (2010) 1.87
Three-dimensional cellular architecture of the flagellar pocket and associated cytoskeleton in trypanosomes revealed by electron microscope tomography. J Cell Sci (2009) 1.82
Dyneins across eukaryotes: a comparative genomic analysis. Traffic (2007) 1.81
The calpains: modular designs and functional diversity. Genome Biol (2007) 1.80
Molecular evolution of FtsZ protein sequences encoded within the genomes of archaea, bacteria, and eukaryota. J Mol Evol (2004) 1.65
A "holistic" kinesin phylogeny reveals new kinesin families and predicts protein functions. Mol Biol Cell (2006) 1.59
Variant surface glycoprotein RNA interference triggers a precytokinesis cell cycle arrest in African trypanosomes. Proc Natl Acad Sci U S A (2005) 1.58
Nesprin-2 interacts with meckelin and mediates ciliogenesis via remodelling of the actin cytoskeleton. J Cell Sci (2009) 1.58
The heart of darkness: growth and form of Trypanosoma brucei in the tsetse fly. Trends Parasitol (2009) 1.54
Chromosome-wide analysis of gene function by RNA interference in the african trypanosome. Eukaryot Cell (2006) 1.47
A repetitive protein essential for the flagellum attachment zone filament structure and function in Trypanosoma brucei. Protist (2007) 1.44
Asymmetric cell division as a route to reduction in cell length and change in cell morphology in trypanosomes. Protist (2007) 1.43
The flagella connector of Trypanosoma brucei: an unusual mobile transmembrane junction. J Cell Sci (2004) 1.43
More than one way to build a flagellum: comparative genomics of parasitic protozoa. Curr Biol (2004) 1.36
The DNA sequence of chromosome I of an African trypanosome: gene content, chromosome organisation, recombination and polymorphism. Nucleic Acids Res (2003) 1.33
A novel ISWI is involved in VSG expression site downregulation in African trypanosomes. EMBO J (2007) 1.33
Trypanosome IFT mutants provide insight into the motor location for mobility of the flagella connector and flagellar membrane formation. J Cell Sci (2006) 1.29
Cell biology of the trypanosome genome. Microbiol Mol Biol Rev (2010) 1.27
An essential quality control mechanism at the eukaryotic basal body prior to intraflagellar transport. Traffic (2007) 1.27
The paraflagellar rod of kinetoplastid parasites: from structure to components and function. Int J Parasitol (2009) 1.25
The evolution of the cytoskeleton. J Cell Biol (2011) 1.22
The Parkin co-regulated gene product, PACRG, is an evolutionarily conserved axonemal protein that functions in outer-doublet microtubule morphogenesis. J Cell Sci (2005) 1.21
The cell cycle of Leishmania: morphogenetic events and their implications for parasite biology. Mol Microbiol (2010) 1.21
The small chromosomes of Trypanosoma brucei involved in antigenic variation are constructed around repetitive palindromes. Genome Res (2004) 1.20
Combining RNA interference mutants and comparative proteomics to identify protein components and dependences in a eukaryotic flagellum. J Biol Chem (2008) 1.19
Patterns of kinesin evolution reveal a complex ancestral eukaryote with a multifunctional cytoskeleton. BMC Evol Biol (2010) 1.17
Gamma-tubulin functions in the nucleation of a discrete subset of microtubules in the eukaryotic flagellum. Curr Biol (2003) 1.17
Basal body movements orchestrate membrane organelle division and cell morphogenesis in Trypanosoma brucei. J Cell Sci (2010) 1.14
The kinetoplast duplication cycle in Trypanosoma brucei is orchestrated by cytoskeleton-mediated cell morphogenesis. Mol Cell Biol (2010) 1.14
A meckelin-filamin A interaction mediates ciliogenesis. Hum Mol Genet (2011) 1.12
Basal body and flagellum mutants reveal a rotational constraint of the central pair microtubules in the axonemes of trypanosomes. J Cell Sci (2006) 1.11
The hydrocephalus inducing gene product, Hydin, positions axonemal central pair microtubules. BMC Biol (2007) 1.11
Distinct and overlapping roles for two Dicer-like proteins in the RNA interference pathways of the ancient eukaryote Trypanosoma brucei. Proc Natl Acad Sci U S A (2009) 1.11
Diversification of function by different isoforms of conventionally shared RNA polymerase subunits. Mol Biol Cell (2007) 1.11
Evolutionary relationships and protein domain architecture in an expanded calpain superfamily in kinetoplastid parasites. J Mol Evol (2005) 1.10
Repetitive elements in genomes of parasitic protozoa. Microbiol Mol Biol Rev (2003) 1.09
Membrane domains and flagellar pocket boundaries are influenced by the cytoskeleton in African trypanosomes. Proc Natl Acad Sci U S A (2009) 1.08
Integration of pZJM library plasmids into unexpected locations in the Trypanosoma brucei genome. Mol Biochem Parasitol (2004) 1.08
Cytokinesis in Trypanosoma brucei differs between bloodstream and tsetse trypomastigote forms: implications for microtubule-based morphogenesis and mutant analysis. Mol Microbiol (2013) 1.04
Flagellar and ciliary beating in trypanosome motility. Cell Motil Cytoskeleton (2007) 1.04
Beyond 9+0: noncanonical axoneme structures characterize sensory cilia from protists to humans. FASEB J (2010) 1.04
Protein targeting of an unusual, evolutionarily conserved adenylate kinase to a eukaryotic flagellum. Mol Biol Cell (2004) 1.03
Genome-wide analysis reveals extensive functional interaction between DNA replication initiation and transcription in the genome of Trypanosoma brucei. Cell Rep (2012) 1.01
The trypanosome flagellum. J Cell Sci (2003) 1.01
The FACT subunit TbSpt16 is involved in cell cycle specific control of VSG expression sites in Trypanosoma brucei. Mol Microbiol (2010) 0.98
Structural asymmetry and discrete nucleic acid subdomains in the Trypanosoma brucei kinetoplast. Mol Microbiol (2007) 0.98
A new generation of T7 RNA polymerase-independent inducible expression plasmids for Trypanosoma brucei. PLoS One (2012) 0.98
The limits on trypanosomatid morphological diversity. PLoS One (2013) 0.98
A SAS-6-like protein suggests that the Toxoplasma conoid complex evolved from flagellar components. Eukaryot Cell (2013) 0.97
Histone H1 plays a role in heterochromatin formation and VSG expression site silencing in Trypanosoma brucei. PLoS Pathog (2012) 0.97
An evolutionarily conserved coiled-coil protein implicated in polycystic kidney disease is involved in basal body duplication and flagellar biogenesis in Trypanosoma brucei. Mol Cell Biol (2005) 0.97
The structural mechanics of cell division in Trypanosoma brucei. Biochem Soc Trans (2008) 0.97
Evolution of tubulin gene arrays in Trypanosomatid parasites: genomic restructuring in Leishmania. BMC Genomics (2006) 0.96
Cryo-electron tomography and 3-D analysis of the intact flagellum in Trypanosoma brucei. J Struct Biol (2012) 0.96
NLP is a novel transcription regulator involved in VSG expression site control in Trypanosoma brucei. Nucleic Acids Res (2010) 0.96
Evolutionary cell biology of chromosome segregation: insights from trypanosomes. Open Biol (2013) 0.95
Cryptic paraflagellar rod in endosymbiont-containing kinetoplastid protozoa. Eukaryot Cell (2005) 0.95
Perturbation of phosphatidylethanolamine synthesis affects mitochondrial morphology and cell-cycle progression in procyclic-form Trypanosoma brucei. Mol Microbiol (2009) 0.95
Cell morphogenesis of Trypanosoma brucei requires the paralogous, differentially expressed calpain-related proteins CAP5.5 and CAP5.5V. Protist (2009) 0.94
Intracellular positioning of isoforms explains an unusually large adenylate kinase gene family in the parasite Trypanosoma brucei. J Biol Chem (2005) 0.94
Detailed interrogation of trypanosome cell biology via differential organelle staining and automated image analysis. BMC Biol (2012) 0.94
A protein-protein interaction map of the Trypanosoma brucei paraflagellar rod. PLoS One (2009) 0.94
Blocking variant surface glycoprotein synthesis in Trypanosoma brucei triggers a general arrest in translation initiation. PLoS One (2009) 0.94
The evolution of land plant cilia. New Phytol (2012) 0.94
RNA interference mutant induction in vivo demonstrates the essential nature of trypanosome flagellar function during mammalian infection. Eukaryot Cell (2007) 0.94
Expression and cellular localisation of calpain-like proteins in Trypanosoma brucei. Mol Biochem Parasitol (2009) 0.93
Functional characterization of cohesin SMC3 and separase and their roles in the segregation of large and minichromosomes in Trypanosoma brucei. Mol Microbiol (2009) 0.93
Conservation of ciliary proteins in plants with no cilia. BMC Plant Biol (2011) 0.91
A new function of Trypanosoma brucei mitochondrial topoisomerase II is to maintain kinetoplast DNA network topology. Mol Microbiol (2008) 0.91
WCB is a C2 domain protein defining the plasma membrane - sub-pellicular microtubule corset of kinetoplastid parasites. Protist (2007) 0.90
Functional characterisation and drug target validation of a mitotic kinesin-13 in Trypanosoma brucei. PLoS Pathog (2010) 0.90
Functional characterization of cohesin subunit SCC1 in Trypanosoma brucei and dissection of mutant phenotypes in two life cycle stages. Mol Microbiol (2008) 0.90
Relationships between the major kinetoplastid paraflagellar rod proteins: a consolidating nomenclature. Mol Biochem Parasitol (2004) 0.90
Calmodulin is required for paraflagellar rod assembly and flagellum-cell body attachment in trypanosomes. Protist (2013) 0.90
The Argonaute protein TbAGO1 contributes to large and mini-chromosome segregation and is required for control of RIME retroposons and RHS pseudogene-associated transcripts. Mol Biochem Parasitol (2007) 0.89
The expanded Kinesin-13 repertoire of trypanosomes contains only one mitotic Kinesin indicating multiple extra-nuclear roles. PLoS One (2010) 0.89
Modes of flagellar assembly in Chlamydomonas reinhardtii and Trypanosoma brucei. Elife (2014) 0.88
A cell-body groove housing the new flagellum tip suggests an adaptation of cellular morphogenesis for parasitism in the bloodstream form of Trypanosoma brucei. J Cell Sci (2013) 0.88
Analysis of the trypanosome flagellar proteome using a combined electron transfer/collisionally activated dissociation strategy. J Am Soc Mass Spectrom (2008) 0.88
Cytokinesis in trypanosomes. Cytoskeleton (Hoboken) (2012) 0.87
Ultrastructural investigation methods for Trypanosoma brucei. Methods Cell Biol (2010) 0.87
Bioinformatic insights to the ESAG5 and GRESAG5 gene families in kinetoplastid parasites. Mol Biochem Parasitol (2008) 0.87
A post-assembly structural modification to the lumen of flagellar microtubule doublets. Curr Biol (2006) 0.86
Functional studies of an evolutionarily conserved, cytochrome b5 domain protein reveal a specific role in axonemal organisation and the general phenomenon of post-division axonemal growth in trypanosomes. Cell Motil Cytoskeleton (2009) 0.86
Identification of a crenarchaeal orthologue of Elf1: implications for chromatin and transcription in Archaea. Biol Direct (2009) 0.86
Ab initio identification of novel regulatory elements in the genome of Trypanosoma brucei by Bayesian inference on sequence segmentation. PLoS One (2011) 0.85
Identification and characterization of two trypanosome TFIIS proteins exhibiting particular domain architectures and differential nuclear localizations. Mol Microbiol (2008) 0.85
Depletion of mitochondrial acyl carrier protein in bloodstream-form Trypanosoma brucei causes a kinetoplast segregation defect. Eukaryot Cell (2011) 0.85