Published in J Am Chem Soc on June 09, 2004
Protein structure determination in living cells by in-cell NMR spectroscopy. Nature (2009) 1.71
Quantitative NMR analysis of the protein G B1 domain in Xenopus laevis egg extracts and intact oocytes. Proc Natl Acad Sci U S A (2006) 1.70
Differential dynamical effects of macromolecular crowding on an intrinsically disordered protein and a globular protein: implications for in-cell NMR spectroscopy. J Am Chem Soc (2008) 1.49
Protein (19)F NMR in Escherichia coli. J Am Chem Soc (2010) 1.46
Protein nuclear magnetic resonance under physiological conditions. Biochemistry (2009) 1.23
In-cell NMR spectroscopy of proteins inside Xenopus laevis oocytes. J Biomol NMR (2006) 1.10
A new labeling method for methyl transverse relaxation-optimized spectroscopy NMR spectra of alanine residues. J Am Chem Soc (2007) 1.08
Screening of small molecule interactor library by using in-cell NMR spectroscopy (SMILI-NMR). J Med Chem (2009) 1.05
Site-specific labeling of proteins with NMR-active unnatural amino acids. J Biomol NMR (2009) 1.00
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NMR protein structure determination in living E. coli cells using nonlinear sampling. Nat Protoc (2010) 0.87
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Specific ion effects on macromolecular interactions in Escherichia coli extracts. Protein Sci (2014) 0.84
High-resolution magic angle spinning nuclear magnetic resonance analysis of metabolic changes in melanoma cells after induction of melanogenesis. Anal Biochem (2008) 0.84
Suppression of phospholipid biosynthesis by cerulenin in the condensed Single-Protein-Production (cSPP) system. J Biomol NMR (2011) 0.82
A Unique Tool for Cellular Structural Biology: In-cell NMR. J Biol Chem (2015) 0.81
Is buffer a good proxy for a crowded cell-like environment? A comparative NMR study of calmodulin side-chain dynamics in buffer and E. coli lysate. PLoS One (2012) 0.81
In-cell ¹³C NMR spectroscopy for the study of intrinsically disordered proteins. Nat Protoc (2014) 0.80
A new carbamidemethyl-linked lanthanoid chelating tag for PCS NMR spectroscopy of proteins in living HeLa cells. J Biomol NMR (2016) 0.76
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Combined Use of Oligopeptides, Fragment Libraries, and Natural Compounds: A Comprehensive Approach To Sample the Druggability of Vascular Endothelial Growth Factor. ChemMedChem (2015) 0.75
Nix is a selective autophagy receptor for mitochondrial clearance. EMBO Rep (2009) 5.52
Phosphorylation of the autophagy receptor optineurin restricts Salmonella growth. Science (2011) 5.46
Global landscape of HIV-human protein complexes. Nature (2011) 3.70
E3-independent monoubiquitination of ubiquitin-binding proteins. Mol Cell (2007) 2.77
Vif hijacks CBF-β to degrade APOBEC3G and promote HIV-1 infection. Nature (2011) 2.71
Local protease signaling contributes to neural tube closure in the mouse embryo. Dev Cell (2010) 2.19
NES consensus redefined by structures of PKI-type and Rev-type nuclear export signals bound to CRM1. Nat Struct Mol Biol (2010) 2.12
Substrate profiling of cysteine proteases using a combinatorial peptide library identifies functionally unique specificities. J Biol Chem (2006) 2.05
Loss of p63 and its microRNA-205 target results in enhanced cell migration and metastasis in prostate cancer. Proc Natl Acad Sci U S A (2012) 1.97
Low-conductivity buffers for high-sensitivity NMR measurements. J Am Chem Soc (2002) 1.88
Trapping moving targets with small molecules. Science (2009) 1.86
Mycobacterium tuberculosis MycP1 protease plays a dual role in regulation of ESX-1 secretion and virulence. Cell Host Microbe (2010) 1.76
A C-terminal inhibitory domain controls the activity of p63 by an intramolecular mechanism. Mol Cell Biol (2002) 1.73
Engineering of a macromolecular scaffold to develop specific protease inhibitors. Nat Biotechnol (2003) 1.73
Structural basis for tail-anchored membrane protein biogenesis by the Get3-receptor complex. Science (2011) 1.68
Adhesion signaling by a novel mitotic substrate of src kinases. Oncogene (2005) 1.68
Structural basis for the selectivity of the external thioesterase of the surfactin synthetase. Nature (2008) 1.66
Actin binding to the central domain of WASP/Scar proteins plays a critical role in the activation of the Arp2/3 complex. J Biol Chem (2005) 1.64
Conformational switches modulate protein interactions in peptide antibiotic synthetases. Science (2006) 1.61
Coordinate expression and functional profiling identify an extracellular proteolytic signaling pathway. Proc Natl Acad Sci U S A (2007) 1.52
Preparative scale expression of membrane proteins in Escherichia coli-based continuous exchange cell-free systems. Nat Protoc (2007) 1.52
Hepatocyte growth factor is a preferred in vitro substrate for human hepsin, a membrane-anchored serine protease implicated in prostate and ovarian cancers. Biochem J (2005) 1.51
Involvement of the ubiquitin-like domain of TBK1/IKK-i kinases in regulation of IFN-inducible genes. EMBO J (2007) 1.51
High level cell-free expression and specific labeling of integral membrane proteins. Eur J Biochem (2004) 1.45
Plasminogen activation independent of uPA and tPA maintains wound healing in gene-deficient mice. EMBO J (2006) 1.42
A multi-enzyme cascade of hemoglobin proteolysis in the intestine of blood-feeding hookworms. J Biol Chem (2004) 1.41
Structural evolution of C-terminal domains in the p53 family. EMBO J (2007) 1.32
Specificity profiling of seven human tissue kallikreins reveals individual subsite preferences. J Biol Chem (2006) 1.31
Catalytic properties and inhibition of proline-specific dipeptidyl peptidases II, IV and VII. Biochem J (2003) 1.29
Gain-of-function mutation in ADULT syndrome reveals the presence of a second transactivation domain in p63. Hum Mol Genet (2002) 1.29
In-cell NMR spectroscopy. Methods Enzymol (2005) 1.27
Expedient solid-phase synthesis of fluorogenic protease substrates using the 7-amino-4-carbamoylmethylcoumarin (ACC) fluorophore. J Org Chem (2002) 1.26
Fabs enable single particle cryoEM studies of small proteins. Structure (2012) 1.25
Continuous imaging of plasmon rulers in live cells reveals early-stage caspase-3 activation at the single-molecule level. Proc Natl Acad Sci U S A (2009) 1.25
DNA damage in oocytes induces a switch of the quality control factor TAp63α from dimer to tetramer. Cell (2011) 1.25
Cell-free production of G protein-coupled receptors for functional and structural studies. J Struct Biol (2007) 1.24
Cercarial elastase is encoded by a functionally conserved gene family across multiple species of schistosomes. J Biol Chem (2002) 1.22
Elimination of 13Calpha splitting in protein NMR spectra by deconvolution with maximum entropy reconstruction. J Am Chem Soc (2003) 1.21
Inhibition of a viral enzyme by a small-molecule dimer disruptor. Nat Chem Biol (2009) 1.21
Structural and functional relationships in the virulence-associated cathepsin L proteases of the parasitic liver fluke, Fasciola hepatica. J Biol Chem (2007) 1.19
Vinyl sulfones as antiparasitic agents and a structural basis for drug design. J Biol Chem (2009) 1.18
Isotope-coded and affinity-tagged cross-linking (ICATXL): an efficient strategy to probe protein interaction surfaces. J Am Chem Soc (2006) 1.17
Altered substrate specificity of drug-resistant human immunodeficiency virus type 1 protease. J Virol (2002) 1.17
Investigating macromolecules inside cultured and injected cells by in-cell NMR spectroscopy. Nat Protoc (2006) 1.16
Granzyme B proteolyzes receptors important to proliferation and survival, tipping the balance toward apoptosis. J Biol Chem (2006) 1.16
Solution NMR structure of proteorhodopsin. Angew Chem Int Ed Engl (2011) 1.15
Global identification of peptidase specificity by multiplex substrate profiling. Nat Methods (2012) 1.14
Structure and ligand binding of the extended Tudor domain of D. melanogaster Tudor-SN. J Mol Biol (2009) 1.14
Segmental isotopic labeling of a central domain in a multidomain protein by protein trans-splicing using only one robust DnaE intein. Angew Chem Int Ed Engl (2009) 1.13
Potent and selective inhibition of membrane-type serine protease 1 by human single-chain antibodies. Biochemistry (2003) 1.12
Selective chemical functional probes of granzymes A and B reveal granzyme B is a major effector of natural killer cell-mediated lysis of target cells. Chem Biol (2005) 1.12
Differential altered stability and transcriptional activity of ΔNp63 mutants in distinct ectodermal dysplasias. J Cell Sci (2011) 1.11
Characterization of the interaction of GABARAPL-1 with the LIR motif of NBR1. J Mol Biol (2011) 1.11
The parallel G-quadruplex structure of vertebrate telomeric repeat sequences is not the preferred folding topology under physiological conditions. Nucleic Acids Res (2011) 1.11
Quantitative identification of the protonation state of histidines in vitro and in vivo. Biochemistry (2003) 1.11
Cell-free expression as an emerging technique for the large scale production of integral membrane protein. FEBS J (2006) 1.10
Structural investigation of the C-terminal catalytic fragment of presenilin 1. Proc Natl Acad Sci U S A (2010) 1.10
Collagen-binding mode of vWF-A3 domain determined by a transferred cross-saturation experiment. Nat Struct Biol (2003) 1.10
The energetic conversion competence of Escherichia coli during aerobic respiration studied by 31P NMR using a circulating fermentation system. J Biochem (2004) 1.10
Selective inhibition of the collagenolytic activity of human cathepsin K by altering its S2 subsite specificity. Biochemistry (2002) 1.10
The mechanism of inhibition of antibody-based inhibitors of membrane-type serine protease 1 (MT-SP1). J Mol Biol (2007) 1.10
Structure of an Fab-protease complex reveals a highly specific non-canonical mechanism of inhibition. J Mol Biol (2008) 1.09
Biochemical and NMR analyses of an SF3b155-p14-U2AF-RNA interaction network involved in branch point definition during pre-mRNA splicing. RNA (2006) 1.09
A parasite cysteine protease is key to host protein degradation and iron acquisition. J Biol Chem (2008) 1.06
The periplasmic serine protease inhibitor ecotin protects bacteria against neutrophil elastase. Biochem J (2004) 1.05
Antagonistic anti-urokinase plasminogen activator receptor (uPAR) antibodies significantly inhibit uPAR-mediated cellular signaling and migration. J Biol Chem (2010) 1.04
Prediction of protease substrates using sequence and structure features. Bioinformatics (2010) 1.04
The oligomeric structure of human granzyme A is a determinant of its extended substrate specificity. Nat Struct Biol (2003) 1.04
A methylation-dependent electrostatic switch controls DNA repair and transcriptional activation by E. coli ada. Mol Cell (2005) 1.03
Structural basis for phosphorylation-triggered autophagic clearance of Salmonella. Biochem J (2013) 1.03
Communication between the active sites and dimer interface of a herpesvirus protease revealed by a transition-state inhibitor. Proc Natl Acad Sci U S A (2004) 1.03
GPS-Prot: a web-based visualization platform for integrating host-pathogen interaction data. BMC Bioinformatics (2011) 1.03
A novel Entamoeba histolytica cysteine proteinase, EhCP4, is key for invasive amebiasis and a therapeutic target. J Biol Chem (2010) 1.03
Hemoglobin cleavage site-specificity of the Plasmodium falciparum cysteine proteases falcipain-2 and falcipain-3. PLoS One (2009) 1.02
Critical role of amino acid 23 in mediating activity and specificity of vinckepain-2, a papain-family cysteine protease of rodent malaria parasites. Biochem J (2002) 1.02
Production of membrane proteins using cell-free expression systems. Proteomics (2008) 1.02
Cell free expression and functional reconstitution of eukaryotic drug transporters. Biochemistry (2008) 1.01
The guanylate kinase domain of the MAGUK PSD-95 binds dynamically to a conserved motif in MAP1a. Nat Struct Mol Biol (2007) 1.01
IrAE: an asparaginyl endopeptidase (legumain) in the gut of the hard tick Ixodes ricinus. Int J Parasitol (2007) 1.01
Caspase-2 is an initiator caspase responsible for pore-forming toxin-mediated apoptosis. EMBO J (2012) 1.01
Efficient strategy for the rapid backbone assignment of membrane proteins. J Am Chem Soc (2005) 1.01
Global substrate profiling of proteases in human neutrophil extracellular traps reveals consensus motif predominantly contributed by elastase. PLoS One (2013) 1.00
Substrates of the prostate-specific serine protease prostase/KLK4 defined by positional-scanning peptide libraries. Prostate (2005) 0.99
Molecular crowding drives active Pin1 into nonspecific complexes with endogenous proteins prior to substrate recognition. J Am Chem Soc (2013) 0.99
Structures and specificity of the human kallikrein-related peptidases KLK 4, 5, 6, and 7. Biol Chem (2008) 0.99
Papa's got a brand new tag: advances in identification of proteases and their substrates. Trends Biotechnol (2005) 0.99
Development of alpha-keto-based inhibitors of cruzain, a cysteine protease implicated in Chagas disease. Bioorg Med Chem (2005) 0.98
Quantitation of membrane type serine protease 1 (MT-SP1) in transformed and normal cells. Biol Chem (2003) 0.98
Cell-free production of integral membrane proteins on a preparative scale. Methods Mol Biol (2007) 0.98
Granzyme M is a regulatory protease that inactivates proteinase inhibitor 9, an endogenous inhibitor of granzyme B. J Biol Chem (2004) 0.98
Substrate specificity of MarP, a periplasmic protease required for resistance to acid and oxidative stress in Mycobacterium tuberculosis. J Biol Chem (2013) 0.97
A method for integrative structure determination of protein-protein complexes. Bioinformatics (2012) 0.97
Urokinase-type plasminogen activator receptor (uPAR) ligation induces a raft-localized integrin signaling switch that mediates the hypermotile phenotype of fibrotic fibroblasts. J Biol Chem (2014) 0.97