Published in Proc Natl Acad Sci U S A on May 05, 2010
Three-dimensional structure of human γ-secretase. Nature (2014) 2.84
Presenilins and γ-secretase: structure, function, and role in Alzheimer Disease. Cold Spring Harb Perspect Med (2012) 2.02
Structure of a presenilin family intramembrane aspartate protease. Nature (2012) 1.76
Facile backbone structure determination of human membrane proteins by NMR spectroscopy. Nat Methods (2012) 1.16
Solution NMR structure of proteorhodopsin. Angew Chem Int Ed Engl (2011) 1.15
Crystal structure of the γ-secretase component nicastrin. Proc Natl Acad Sci U S A (2014) 1.13
Structural basis of human γ-secretase assembly. Proc Natl Acad Sci U S A (2015) 1.13
Objective identification of residue ranges for the superposition of protein structures. BMC Bioinformatics (2011) 1.09
Mutagenesis mapping of the presenilin 1 calcium leak conductance pore. J Biol Chem (2011) 1.05
Presenilins function in ER calcium leak and Alzheimer's disease pathogenesis. Cell Calcium (2011) 0.99
Functional and topological analysis of Pen-2, the fourth subunit of the gamma-secretase complex. J Biol Chem (2011) 0.97
Generation of Alzheimer disease-associated amyloid β42/43 peptide by γ-secretase can be inhibited directly by modulation of membrane thickness. J Biol Chem (2012) 0.94
Contribution of the γ-secretase subunits to the formation of catalytic pore of presenilin 1 protein. J Biol Chem (2012) 0.93
Structural biology of presenilin 1 complexes. Mol Neurodegener (2014) 0.88
Toward the structure of presenilin/γ-secretase and presenilin homologs. Biochim Biophys Acta (2013) 0.87
Polymer-based cell-free expression of ligand-binding family B G-protein coupled receptors without detergents. Protein Sci (2011) 0.86
Structural biology of presenilins and signal peptide peptidases. J Biol Chem (2013) 0.85
The very many faces of presenilins and the γ-secretase complex. Protoplasma (2013) 0.82
The γ-secretase complex: from structure to function. Front Cell Neurosci (2014) 0.81
Changed membrane integration and catalytic site conformation are two mechanisms behind the increased Aβ42/Aβ40 ratio by presenilin 1 familial Alzheimer-linked mutations. FEBS Open Bio (2014) 0.78
Presenilin transmembrane domain 8 conserved AXXXAXXXG motifs are required for the activity of the γ-secretase complex. J Biol Chem (2015) 0.77
Functional analysis and purification of a Pen-2 fusion protein for γ-secretase structural studies. J Neurochem (2014) 0.76
A simple method for displaying the hydropathic character of a protein. J Mol Biol (1982) 150.65
Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes. J Mol Biol (2001) 66.87
MOLMOL: a program for display and analysis of macromolecular structures. J Mol Graph (1996) 39.73
GROMACS: fast, flexible, and free. J Comput Chem (2005) 27.22
The MARTINI force field: coarse grained model for biomolecular simulations. J Phys Chem B (2007) 9.56
Automated NMR structure calculation with CYANA. Methods Mol Biol (2004) 9.31
Twenty years of the Alzheimer's disease amyloid hypothesis: a genetic perspective. Cell (2005) 8.40
Two transmembrane aspartates in presenilin-1 required for presenilin endoproteolysis and gamma-secretase activity. Nature (1999) 8.28
Structure and mechanism of the M2 proton channel of influenza A virus. Nature (2008) 6.79
Oligomeric and fibrillar species of amyloid-beta peptides differentially affect neuronal viability. J Biol Chem (2002) 5.63
Characterization of membrane proteins in detergent solutions. Biochim Biophys Acta (1976) 4.84
Utilization of site-directed spin labeling and high-resolution heteronuclear nuclear magnetic resonance for global fold determination of large proteins with limited nuclear overhauser effect data. Biochemistry (2000) 4.52
Crystal structure of a rhomboid family intramembrane protease. Nature (2006) 3.37
Interhelical hydrogen bonding drives strong interactions in membrane proteins. Nat Struct Biol (2000) 3.12
beta-Amyloid-(1-42) is a major component of cerebrovascular amyloid deposits: implications for the pathology of Alzheimer disease. Proc Natl Acad Sci U S A (1993) 2.80
NMR structure of Mistic, a membrane-integrating protein for membrane protein expression. Science (2005) 2.41
X-ray structure of EmrE supports dual topology model. Proc Natl Acad Sci U S A (2007) 2.34
Solution nuclear magnetic resonance structure of membrane-integral diacylglycerol kinase. Science (2009) 2.30
Effective energy function for proteins in lipid membranes. Proteins (2003) 2.27
Insertion and assembly of membrane proteins via simulation. J Am Chem Soc (2006) 2.12
Soluble fibrillar oligomer levels are elevated in Alzheimer's disease brain and correlate with cognitive dysfunction. Neurobiol Dis (2009) 2.12
Structure of a site-2 protease family intramembrane metalloprotease. Science (2007) 2.08
NMR study of the tetrameric KcsA potassium channel in detergent micelles. Protein Sci (2006) 2.07
An evaluation of detergents for NMR structural studies of membrane proteins. J Biomol NMR (2004) 1.99
Protein topology of presenilin 1. Neuron (1996) 1.94
NMR solution structure of the integral membrane enzyme DsbB: functional insights into DsbB-catalyzed disulfide bond formation. Mol Cell (2008) 1.92
Electron microscopic structure of purified, active gamma-secretase reveals an aqueous intramembrane chamber and two pores. Proc Natl Acad Sci U S A (2006) 1.77
Cryoelectron microscopy structure of purified gamma-secretase at 12 A resolution. J Mol Biol (2008) 1.75
Site-directed parallel spin-labeling and paramagnetic relaxation enhancement in structure determination of membrane proteins by solution NMR spectroscopy. J Am Chem Soc (2006) 1.73
Active gamma-secretase complexes contain only one of each component. J Biol Chem (2007) 1.69
Structure of the catalytic pore of gamma-secretase probed by the accessibility of substituted cysteines. J Neurosci (2006) 1.66
Presenilin-1 maintains a nine-transmembrane topology throughout the secretory pathway. J Biol Chem (2006) 1.55
Contribution of presenilin transmembrane domains 6 and 7 to a water-containing cavity in the gamma-secretase complex. J Biol Chem (2006) 1.52
A nine-transmembrane domain topology for presenilin 1. J Biol Chem (2005) 1.52
Preparative scale expression of membrane proteins in Escherichia coli-based continuous exchange cell-free systems. Nat Protoc (2007) 1.52
Effect of detergents on the association of the glycophorin a transmembrane helix. Biophys J (2003) 1.51
A novel transmembrane topology of presenilin based on reconciling experimental and computational evidence. FEBS J (2005) 1.42
NMR structure determination of a membrane protein with two transmembrane helices in micelles: MerF of the bacterial mercury detoxification system. Biochemistry (2005) 1.39
Membrane topology of the C. elegans SEL-12 presenilin. Neuron (1996) 1.38
The C-terminal PAL motif and transmembrane domain 9 of presenilin 1 are involved in the formation of the catalytic pore of the gamma-secretase. J Neurosci (2008) 1.30
C-terminal PAL motif of presenilin and presenilin homologues required for normal active site conformation. J Neurochem (2005) 1.27
The presenilin C-terminus is required for ER-retention, nicastrin-binding and gamma-secretase activity. EMBO J (2004) 1.26
Speeding up parallel GROMACS on high-latency networks. J Comput Chem (2007) 1.23
Structure of the Na,K-ATPase regulatory protein FXYD1 in micelles. Biochemistry (2007) 1.23
Evidence for a six-transmembrane domain structure of presenilin 1. J Biol Chem (1997) 1.17
Additional evidence for an eight-transmembrane-domain topology for Caenorhabditis elegans and human presenilins. Proc Natl Acad Sci U S A (1998) 1.17
Transmembrane domain 9 of presenilin determines the dynamic conformation of the catalytic site of gamma-secretase. J Biol Chem (2008) 1.16
Conserved "PAL" sequence in presenilins is essential for gamma-secretase activity, but not required for formation or stabilization of gamma-secretase complexes. Neurobiol Dis (2004) 1.06
The seven-transmembrane spanning topography of the Alzheimer disease-related presenilin proteins in the plasma membranes of cultured cells. Proc Natl Acad Sci U S A (1997) 1.04
Cell free expression and functional reconstitution of eukaryotic drug transporters. Biochemistry (2008) 1.01
Cell-free production of integral membrane proteins on a preparative scale. Methods Mol Biol (2007) 0.98
The presenilins turned inside out: implications for their structures and functions. Proc Natl Acad Sci U S A (2004) 0.97
Cell-free expression and stable isotope labelling strategies for membrane proteins. J Biomol NMR (2009) 0.95
Mechanism of gamma-secretase cleavage activation: is gamma-secretase regulated through autoinhibition involving the presenilin-1 exon 9 loop? Biochemistry (2004) 0.93
Membrane topology of Alzheimer's disease-related presenilin 1. Evidence for the existence of a molecular species with a seven membrane-spanning and one membrane-embedded structure. J Biol Chem (1999) 0.92
Topology of the C-terminal fragment of human presenilin 1. Biochemistry (2005) 0.91
Transmembrane segment enhanced labeling as a tool for the backbone assignment of alpha-helical membrane proteins. Proc Natl Acad Sci U S A (2008) 0.88
NMR studies of subunit c of the ATP synthase from Propionigenium modestum in dodecylsulphate micelles. Eur J Biochem (1999) 0.85
Stable interactions between the transmembrane domains of the adenosine A2A receptor. Protein Sci (2008) 0.84
Protein NMR structure determination with automated NOE assignment using the new software CANDID and the torsion angle dynamics algorithm DYANA. J Mol Biol (2002) 11.20
Atomic-force microscopy: Rhodopsin dimers in native disc membranes. Nature (2003) 5.85
Nix is a selective autophagy receptor for mitochondrial clearance. EMBO Rep (2009) 5.52
Phosphorylation of the autophagy receptor optineurin restricts Salmonella growth. Science (2011) 5.46
Protein NMR structure determination with automated NOE-identification in the NOESY spectra using the new software ATNOS. J Biomol NMR (2002) 4.55
RECOORD: a recalculated coordinate database of 500+ proteins from the PDB using restraints from the BioMagResBank. Proteins (2005) 3.41
Optimal isotope labelling for NMR protein structure determinations. Nature (2006) 2.89
E3-independent monoubiquitination of ubiquitin-binding proteins. Mol Cell (2007) 2.77
CASD-NMR: critical assessment of automated structure determination by NMR. Nat Methods (2009) 2.50
Oligomerization of G protein-coupled receptors: past, present, and future. Biochemistry (2004) 2.03
Loss of p63 and its microRNA-205 target results in enhanced cell migration and metastasis in prostate cancer. Proc Natl Acad Sci U S A (2012) 1.97
Automated protein structure determination from NMR spectra. J Am Chem Soc (2006) 1.96
Low-conductivity buffers for high-sensitivity NMR measurements. J Am Chem Soc (2002) 1.88
A C-terminal inhibitory domain controls the activity of p63 by an intramolecular mechanism. Mol Cell Biol (2002) 1.73
Protein structure determination in living cells by in-cell NMR spectroscopy. Nature (2009) 1.71
Structural basis for tail-anchored membrane protein biogenesis by the Get3-receptor complex. Science (2011) 1.68
Structural basis for the selectivity of the external thioesterase of the surfactin synthetase. Nature (2008) 1.66
Prion protein NMR structures of cats, dogs, pigs, and sheep. Proc Natl Acad Sci U S A (2005) 1.66
Actin binding to the central domain of WASP/Scar proteins plays a critical role in the activation of the Arp2/3 complex. J Biol Chem (2005) 1.64
Rhodopsin signaling and organization in heterozygote rhodopsin knockout mice. J Biol Chem (2004) 1.64
Conformational switches modulate protein interactions in peptide antibiotic synthetases. Science (2006) 1.61
Recommendations of the wwPDB NMR Validation Task Force. Structure (2013) 1.57
Functional characterization of rhodopsin monomers and dimers in detergents. J Biol Chem (2004) 1.57
Preparative scale expression of membrane proteins in Escherichia coli-based continuous exchange cell-free systems. Nat Protoc (2007) 1.52
Calcium-binding proteins: intracellular sensors from the calmodulin superfamily. Biochem Biophys Res Commun (2002) 1.51
Involvement of the ubiquitin-like domain of TBK1/IKK-i kinases in regulation of IFN-inducible genes. EMBO J (2007) 1.51
The autoregulatory role of EsaR, a quorum-sensing regulator in Pantoea stewartii ssp. stewartii: evidence for a repressor function. Mol Microbiol (2002) 1.51
Structural insight into dimeric interaction of the SARAH domains from Mst1 and RASSF family proteins in the apoptosis pathway. Proc Natl Acad Sci U S A (2007) 1.50
NMR structure of the unliganded Bombyx mori pheromone-binding protein at physiological pH. FEBS Lett (2002) 1.49
NMR structure of the integral membrane protein OmpX. J Mol Biol (2004) 1.46
High level cell-free expression and specific labeling of integral membrane proteins. Eur J Biochem (2004) 1.45
KUJIRA, a package of integrated modules for systematic and interactive analysis of NMR data directed to high-throughput NMR structure studies. J Biomol NMR (2007) 1.44
Maturation of an Escherichia coli ribosomal peptide antibiotic by ATP-consuming N-P bond formation in microcin C7. J Am Chem Soc (2008) 1.41
Prion protein NMR structures of chickens, turtles, and frogs. Proc Natl Acad Sci U S A (2005) 1.34
Structural evolution of C-terminal domains in the p53 family. EMBO J (2007) 1.32
Structural insight into the zinc finger CW domain as a histone modification reader. Structure (2010) 1.30
Gain-of-function mutation in ADULT syndrome reveals the presence of a second transactivation domain in p63. Hum Mol Genet (2002) 1.29
In-cell NMR spectroscopy. Methods Enzymol (2005) 1.27
DNA damage in oocytes induces a switch of the quality control factor TAp63α from dimer to tetramer. Cell (2011) 1.25
Cell-free production of G protein-coupled receptors for functional and structural studies. J Struct Biol (2007) 1.24
Methyl groups as probes for proteins and complexes in in-cell NMR experiments. J Am Chem Soc (2004) 1.23
The supramolecular structure of the GPCR rhodopsin in solution and native disc membranes. Mol Membr Biol (2005) 1.23
Structural basis for the sequence-specific RNA-recognition mechanism of human CUG-BP1 RRM3. Nucleic Acids Res (2009) 1.23
Binding of a designed substrate analogue to diisopropyl fluorophosphatase: implications for the phosphotriesterase mechanism. J Am Chem Soc (2006) 1.23
Blind testing of routine, fully automated determination of protein structures from NMR data. Structure (2012) 1.23
ATP-induced conformational changes of the nucleotide-binding domain of Na,K-ATPase. Nat Struct Biol (2003) 1.23
Elimination of 13Calpha splitting in protein NMR spectra by deconvolution with maximum entropy reconstruction. J Am Chem Soc (2003) 1.21
A naturally occurring mutation of the opsin gene (T4R) in dogs affects glycosylation and stability of the G protein-coupled receptor. J Biol Chem (2004) 1.19
Filament structure, organization, and dynamics in MreB sheets. J Biol Chem (2010) 1.18
Investigating macromolecules inside cultured and injected cells by in-cell NMR spectroscopy. Nat Protoc (2006) 1.16
Solution NMR structure of proteorhodopsin. Angew Chem Int Ed Engl (2011) 1.15
Influence of the completeness of chemical shift assignments on NMR structures obtained with automated NOE assignment. J Struct Funct Genomics (2003) 1.14
Atomic-resolution three-dimensional structure of amyloid β fibrils bearing the Osaka mutation. Angew Chem Int Ed Engl (2014) 1.14
Segmental isotopic labeling of a central domain in a multidomain protein by protein trans-splicing using only one robust DnaE intein. Angew Chem Int Ed Engl (2009) 1.13
Solution structure of the RWD domain of the mouse GCN2 protein. Protein Sci (2004) 1.13
A new algorithm for reliable and general NMR resonance assignment. J Am Chem Soc (2012) 1.13
A novel GCAP1 missense mutation (L151F) in a large family with autosomal dominant cone-rod dystrophy (adCORD). Invest Ophthalmol Vis Sci (2005) 1.12
Differential altered stability and transcriptional activity of ΔNp63 mutants in distinct ectodermal dysplasias. J Cell Sci (2011) 1.11
Characterization of the interaction of GABARAPL-1 with the LIR motif of NBR1. J Mol Biol (2011) 1.11
The parallel G-quadruplex structure of vertebrate telomeric repeat sequences is not the preferred folding topology under physiological conditions. Nucleic Acids Res (2011) 1.11
Detecting molecular interactions that stabilize native bovine rhodopsin. J Mol Biol (2006) 1.11
Solution structure of the extraterminal domain of the bromodomain-containing protein BRD4. Protein Sci (2008) 1.11
Quantitative identification of the protonation state of histidines in vitro and in vivo. Biochemistry (2003) 1.11
Cell-free expression as an emerging technique for the large scale production of integral membrane protein. FEBS J (2006) 1.10
Structure and filament dynamics of the pSK41 actin-like ParM protein: implications for plasmid DNA segregation. J Biol Chem (2010) 1.10
Solution structure and siRNA-mediated knockdown analysis of the mitochondrial disease-related protein C12orf65. Proteins (2012) 1.10
Structures of the first and second double-stranded RNA-binding domains of human TAR RNA-binding protein. Protein Sci (2011) 1.10
Objective identification of residue ranges for the superposition of protein structures. BMC Bioinformatics (2011) 1.09
Structural analysis of the DNA-binding domain of the Erwinia amylovora RcsB protein and its interaction with the RcsAB box. J Biol Chem (2003) 1.07
NMR structure of the R-module: a parallel beta-roll subunit from an Azotobacter vinelandii mannuronan C-5 epimerase. J Biol Chem (2006) 1.05