L-Arginine increases the solubility of unfolded species of hen egg white lysozyme.

PubWeight™: 0.97‹?› | Rank: Top 15%

🔗 View Article (PMC 2253432)

Published in Protein Sci on March 01, 2005

Authors

Ravi Charan Reddy K1, Hauke Lilie, Rainer Rudolph, Christian Lange

Author Affiliations

1: Institut für Biotechnologie, Martin-Luther-Universität Halle/Wittenberg, Kurt-Mothes-Strasse 3, 06120 Halle (Saale), Germany.

Articles citing this

Inhibition of protein aggregation: supramolecular assemblies of arginine hold the key. PLoS One (2007) 1.02

Refolding techniques for recovering biologically active recombinant proteins from inclusion bodies. Biomolecules (2014) 0.98

Effects of solutes on solubilization and refolding of proteins from inclusion bodies with high hydrostatic pressure. Protein Sci (2005) 0.98

Second virial coefficient studies of cosolvent-induced protein self-interaction. Biophys J (2005) 0.96

Investigation of cosolute-protein preferential interaction coefficients: new insight into the mechanism by which arginine inhibits aggregation. J Phys Chem B (2009) 0.86

Ionic liquids as refolding additives: N'-alkyl and N'-(omega-hydroxyalkyl) N-methylimidazolium chlorides. Protein Sci (2005) 0.85

L-arginine hydrochloride increases the solubility of folded and unfolded recombinant plasminogen activator rPA. Protein Sci (2010) 0.84

Arginine and the Hofmeister Series: the role of ion-ion interactions in protein aggregation suppression. J Phys Chem B (2011) 0.83

Characterization of neuronal Src kinase purified from a bacterial expression system. Protein Expr Purif (2010) 0.81

L-arginine mediated renaturation enhances yield of human, α6 Type IV collagen non-collagenous domain from bacterial inclusion bodies. Protein Pept Lett (2012) 0.80

Embryonic stem cell-derived factors inhibit T effector activation and induce T regulatory cells by suppressing PKC-θ activation. PLoS One (2012) 0.79

An optimised system for refolding of human glucose 6-phosphate dehydrogenase. BMC Biotechnol (2009) 0.79

Quantification of anti-aggregation activity of chaperones: a test-system based on dithiothreitol-induced aggregation of bovine serum albumin. PLoS One (2013) 0.76

A small molecule chemical chaperone optimizes its unfolded state contraction and denaturant like properties. Sci Rep (2013) 0.75

Articles cited by this

The folding of hen lysozyme involves partially structured intermediates and multiple pathways. Nature (1992) 4.32

The stabilization of proteins by sucrose. J Biol Chem (1981) 3.34

Formation of three-dimensional structure in proteins. I. Rapid nonenzymic reactivation of reduced lysozyme. Biochemistry (1970) 2.17

Protein stability in mixed solvents: a balance of contact interaction and excluded volume. Biophys J (2003) 2.01

Renaturation, purification and characterization of recombinant Fab-fragments produced in Escherichia coli. Biotechnology (N Y) (1991) 1.88

A kinetic study of the competition between renaturation and aggregation during the refolding of denatured-reduced egg white lysozyme. Biochemistry (1991) 1.83

Protein aggregation in vitro and in vivo: a quantitative model of the kinetic competition between folding and aggregation. Biotechnology (N Y) (1991) 1.65

Fluroescence of proteins in 6-M guanidine hydrochloride. A method for the quantitative determination of tryptophan. Eur J Biochem (1976) 1.54

On the role of surface tension in the stabilization of globular proteins. Protein Sci (1996) 1.50

Inclusion bodies: formation and utilisation. Adv Biochem Eng Biotechnol (2004) 1.46

Practical considerations in refolding proteins from inclusion bodies. Protein Expr Purif (2003) 1.45

Inhibition of aggregation side reactions during in vitro protein folding. Methods Enzymol (1999) 1.45

The thermodynamic mechanism of protein stabilization by trehalose. Biophys Chem (1997) 1.44

Proline inhibits aggregation during protein refolding. Protein Sci (2000) 1.38

The effects of arginine on refolding of aggregated proteins: not facilitate refolding, but suppress aggregation. Biochem Biophys Res Commun (2003) 1.35

Contribution of the surface free energy perturbation to protein-solvent interactions. Biochemistry (1994) 1.29

Increased thermal stability of proteins in the presence of amino acids. Biochem J (1994) 1.27

Biophysical effect of amino acids on the prevention of protein aggregation. J Biochem (2002) 1.24

The pro-sequence facilitates folding of human nerve growth factor from Escherichia coli inclusion bodies. Eur J Biochem (2001) 1.22

Highly efficient recovery of functional single-chain Fv fragments from inclusion bodies overexpressed in Escherichia coli by controlled introduction of oxidizing reagent--application to a human single-chain Fv fragment. J Immunol Methods (1998) 1.21

A new protein folding screen: application to the ligand binding domains of a glutamate and kainate receptor and to lysozyme and carbonic anhydrase. Protein Sci (1999) 1.17

The renaturation of reduced chymotrypsinogen A in guanidine HCl. Refolding versus aggregation. J Biol Chem (1978) 1.13

Preferential hydration and the exclusion of cosolvents from protein surfaces. J Chem Phys (2004) 1.12

The likelihood of aggregation during protein renaturation can be assessed using the second virial coefficient. Protein Sci (2003) 1.11

Control of aggregation in protein refolding: a variety of surfactants promote renaturation of carbonic anhydrase II. Protein Sci (1995) 1.06

Efficient in vitro folding of the three-disulfide derivatives of hen lysozyme in the presence of glycerol. FEBS Lett (1992) 1.04

Oxidative renaturation of lysozyme at high concentrations. Biotechnol Bioeng (1997) 1.01

Comparison of the kinetics of S-S bond, secondary structure, and active site formation during refolding of reduced denatured hen egg white lysozyme. Protein Sci (1999) 1.01

Independent domain folding of Pseudomonas exotoxin and single-chain immunotoxins: influence of interdomain connections. Proc Natl Acad Sci U S A (1992) 0.98

Prevention of thermal inactivation and aggregation of lysozyme by polyamines. Eur J Biochem (2003) 0.96

Refolding and recovery of recombinant human matrix metalloproteinase 7 (matrilysin) from inclusion bodies expressed by Escherichia coli. J Biochem (1999) 0.94

Renaturation of recombinant human neurotrophin-3 from inclusion bodies using a suppressor agent of aggregation. Biotechnol Appl Biochem (1998) 0.88

Antitumor activity of interleukin-21 prepared by novel refolding procedure from inclusion bodies expressed in Escherichia coli. FEBS Lett (2002) 0.86

The effects of lauryl maltoside on the reactivation of several enzymes after treatment with guanidinium chloride. Biochim Biophys Acta (1988) 0.85

The non-ionic detergent Brij 58P mimics chaperone effects. FEBS Lett (2002) 0.80

Implication of the structure and stability of disulfide intermediates of lysozyme on the mechanism of renaturation. Mol Cell Biochem (1982) 0.77

Articles by these authors

Crystal structure of the ligand-bound glucagon-like peptide-1 receptor extracellular domain. J Biol Chem (2008) 2.51

Crystal structure of the incretin-bound extracellular domain of a G protein-coupled receptor. Proc Natl Acad Sci U S A (2007) 2.13

Single-particle tracking of murine polyoma virus-like particles on live cells and artificial membranes. Proc Natl Acad Sci U S A (2005) 2.01

Crystal structure of glucagon-like peptide-1 in complex with the extracellular domain of the glucagon-like peptide-1 receptor. J Biol Chem (2009) 1.94

Passing the baton in class B GPCRs: peptide hormone activation via helix induction? Trends Biochem Sci (2009) 1.89

The neurovascular link in health and disease: molecular mechanisms and therapeutic implications. Neuron (2011) 1.85

Ploidy in cyanobacteria. FEMS Microbiol Lett (2011) 1.70

The redox-switch domain of Hsp33 functions as dual stress sensor. Nat Struct Mol Biol (2007) 1.69

Experimental characterization of Cis-acting elements important for translation and transcription in halophilic archaea. PLoS Genet (2007) 1.63

Stimulation of poly(A) polymerase through a direct interaction with the nuclear poly(A) binding protein allosterically regulated by RNA. EMBO J (2003) 1.55

The antibiotic ADEP reprogrammes ClpP, switching it from a regulated to an uncontrolled protease. EMBO Mol Med (2009) 1.53

Adaptor protein controlled oligomerization activates the AAA+ protein ClpC. EMBO J (2006) 1.49

Primary structure elements of spider dragline silks and their contribution to protein solubility. Biochemistry (2004) 1.48

Identification of a redox-regulated chaperone network. EMBO J (2003) 1.46

Inclusion bodies: formation and utilisation. Adv Biochem Eng Biotechnol (2004) 1.46

VEGF mediates commissural axon chemoattraction through its receptor Flk1. Neuron (2011) 1.45

Characterization of a tightly controlled promoter of the halophilic archaeon Haloferax volcanii and its use in the analysis of the essential cct1 gene. Mol Microbiol (2007) 1.40

Identification of residues in the human guanylate-binding protein 1 critical for nucleotide binding and cooperative GTP hydrolysis. J Mol Biol (2004) 1.35

Atu027, a liposomal small interfering RNA formulation targeting protein kinase N3, inhibits cancer progression. Cancer Res (2008) 1.34

Paclitaxel-coated balloons reduce restenosis after femoro-popliteal angioplasty: evidence from the randomized PACIFIER trial. Circ Cardiovasc Interv (2012) 1.29

Regulatory modes of rod outer segment membrane guanylate cyclase differ in catalytic efficiency and Ca(2+)-sensitivity. Eur J Biochem (2003) 1.24

Crystal structure of 12-oxophytodienoate reductase 3 from tomato: self-inhibition by dimerization. Proc Natl Acad Sci U S A (2006) 1.24

Structural insight into poly(A) binding and catalytic mechanism of human PARN. EMBO J (2005) 1.24

Artificial, non-antibody binding proteins for pharmaceutical and industrial applications. Trends Biotechnol (2005) 1.24

Activation of the redox-regulated chaperone Hsp33 by domain unfolding. J Biol Chem (2004) 1.23

Inhibition of ghrelin action in vitro and in vivo by an RNA-Spiegelmer. Proc Natl Acad Sci U S A (2004) 1.20

Ganglioside-dependent cell attachment and endocytosis of murine polyomavirus-like particles. FEBS Lett (2003) 1.20

Interaction of trigger factor with the ribosome. J Mol Biol (2003) 1.19

The isolated N-terminal domain of the glucagon-like peptide-1 (GLP-1) receptor binds exendin peptides with much higher affinity than GLP-1. J Biol Chem (2003) 1.19

Genome copy numbers and gene conversion in methanogenic archaea. J Bacteriol (2010) 1.18

YcdB from Escherichia coli reveals a novel class of Tat-dependently translocated hemoproteins. J Biol Chem (2006) 1.15

Differential structural properties of GLP-1 and exendin-4 determine their relative affinity for the GLP-1 receptor N-terminal extracellular domain. Biochemistry (2007) 1.14

Catalytic activity and chaperone function of human protein-disulfide isomerase are required for the efficient refolding of proinsulin. J Biol Chem (2001) 1.08

Virologic escape during danoprevir (ITMN-191/RG7227) monotherapy is hepatitis C virus subtype dependent and associated with R155K substitution. Antimicrob Agents Chemother (2011) 1.06

The Drosophila melanogaster Gene cg4930 Encodes a High Affinity Inhibitor for Endonuclease G. J Biol Chem (2009) 1.05

Ca(2+) sensor S100beta-modulated sites of membrane guanylate cyclase in the photoreceptor-bipolar synapse. EMBO J (2002) 1.04

In vitro folding, functional characterization, and disulfide pattern of the extracellular domain of human GLP-1 receptor. Biophys Chem (2002) 1.03

Direct kinetic evidence for half-of-the-sites reactivity in the E1 component of the human pyruvate dehydrogenase multienzyme complex through alternating sites cofactor activation. Biochemistry (2006) 1.03

Quantification of ploidy in proteobacteria revealed the existence of monoploid, (mero-)oligoploid and polyploid species. PLoS One (2011) 1.03

Killing of target cells by redirected granzyme B in the absence of perforin. FEBS Lett (2004) 0.99

Transcriptome changes and cAMP oscillations in an archaeal cell cycle. BMC Cell Biol (2007) 0.98

Electron transfer between yeast cytochrome bc(1) complex and cytochrome c: a structural analysis. Biochim Biophys Acta (2002) 0.97

Trinucleotide expansions leading to an extended poly-L-alanine segment in the poly (A) binding protein PABPN1 cause fibril formation. Protein Sci (2003) 0.95

Dissecting NGF interactions with TrkA and p75 receptors by structural and functional studies of an anti-NGF neutralizing antibody. J Mol Biol (2008) 0.94

RamA and RamB are global transcriptional regulators in Corynebacterium glutamicum and control genes for enzymes of the central metabolism. J Biotechnol (2010) 0.94

Atu027 prevents pulmonary metastasis in experimental and spontaneous mouse metastasis models. Clin Cancer Res (2010) 0.94

Gene expression analysis of Corynebacterium glutamicum subjected to long-term lactic acid adaptation. J Bacteriol (2007) 0.94

Renaturation of human proinsulin--a study on refolding and conversion to insulin. Anal Biochem (2002) 0.93

Crystal structure analysis and solution studies of human Lck-SH3; zinc-induced homodimerization competes with the binding of proline-rich motifs. J Mol Biol (2006) 0.93

Identification of a disulfide bridge essential for transport function of the human proton-coupled amino acid transporter hPAT1. J Biol Chem (2009) 0.92

Structural basis for APPTPPPLPP peptide recognition by the FBP11WW1 domain. J Mol Biol (2005) 0.90

High yield production of recombinant native and modified peptides exemplified by ligands for G-protein coupled receptors. Protein Expr Purif (2008) 0.90

Expansion of embryonic and adult neural stem cells by in utero electroporation or viral stereotaxic injection. J Vis Exp (2012) 0.90

Global analysis of mRNA decay in Halobacterium salinarum NRC-1 at single-gene resolution using DNA microarrays. J Bacteriol (2007) 0.89

Structural insights into an equilibrium folding intermediate of an archaeal ankyrin repeat protein. Proc Natl Acad Sci U S A (2008) 0.89

Disulfide linkage and structure of highly stable yeast-derived virus-like particles of murine polyomavirus. J Biol Chem (2014) 0.88

Structural characterization of the pore forming protein TatAd of the twin-arginine translocase in membranes by solid-state 15N-NMR. Biochim Biophys Acta (2007) 0.88

Biophysical comparison of BMP-2, ProBMP-2, and the free pro-peptide reveals stabilization of the pro-peptide by the mature growth factor. J Biol Chem (2005) 0.88

Characterization of the myristoyl lipid modification of membrane-bound GCAP-2 by 2H solid-state NMR spectroscopy. Biochim Biophys Acta (2007) 0.88

An old target revisited: two new privileged skeletons and an unexpected binding mode for HIV-protease inhibitors. Angew Chem Int Ed Engl (2005) 0.88

Unexpected oligomeric structure of the FocA formate channel of Escherichia coli : a paradigm for the formate-nitrite transporter family of integral membrane proteins. FEMS Microbiol Lett (2009) 0.88

Recombinant expression, in vitro refolding, and biophysical characterization of the human glucagon-like peptide-1 receptor. Biochemistry (2010) 0.87

The unique hexokinase of Kluyveromyces lactis. Molecular and functional characterization and evaluation of a role in glucose signaling. J Biol Chem (2003) 0.87

Suppression of protein aggregation by L-arginine. Curr Pharm Biotechnol (2009) 0.87

The galactose switch in Kluyveromyces lactis depends on nuclear competition between Gal4 and Gal1 for Gal80 binding. J Biol Chem (2006) 0.87

Mechanisms of activity and inhibition of the hepatitis C virus RNA-dependent RNA polymerase. J Biol Chem (2010) 0.86

Folding and association of an extremely stable dimeric protein from Sulfolobus islandicus. J Mol Biol (2004) 0.86

Listeriolysin O as cytotoxic component of an immunotoxin. Protein Sci (2009) 0.86

Affilin-novel binding molecules based on human gamma-B-crystallin, an all beta-sheet protein. J Mol Biol (2007) 0.86

Structural studies on a twin-arginine signal sequence. FEBS Lett (2003) 0.86

The mature part of proNGF induces the structure of its pro-peptide. FEBS Lett (2004) 0.85

Intradomain disulfide bonds impede formation of the alternatively folded state of antibody chains. J Mol Biol (2002) 0.85

Mammalian G-protein-coupled receptor expression in Escherichia coli: I. High-throughput large-scale production as inclusion bodies. Anal Biochem (2008) 0.85

Ionic liquids as refolding additives: N'-alkyl and N'-(omega-hydroxyalkyl) N-methylimidazolium chlorides. Protein Sci (2005) 0.85

Mammalian G protein-coupled receptor expression in Escherichia coli: II. Refolding and biophysical characterization of mouse cannabinoid receptor 1 and human parathyroid hormone receptor 1. Anal Biochem (2010) 0.85

Impact of N-terminal domains for corticotropin-releasing factor (CRF) receptor-ligand interactions. Biochemistry (2005) 0.85

Hexa-histidin tag position influences disulfide structure but not binding behavior of in vitro folded N-terminal domain of rat corticotropin-releasing factor receptor type 2a. Protein Sci (2004) 0.85

Induction of autoimmunity by expansion of autoreactive CD4+CD62Llow cells in vivo. J Immunol (2006) 0.84

Structure and function of a regulated archaeal triosephosphate isomerase adapted to high temperature. J Mol Biol (2004) 0.84

Oligomerization and assembly of the matrix protein of Borna disease virus. FEBS Lett (2005) 0.84

The pro-peptide of proNGF: structure formation and intramolecular association with NGF. Protein Sci (2007) 0.84

Characterization of a dipartite iron uptake system from uropathogenic Escherichia coli strain F11. J Biol Chem (2011) 0.84

L-arginine hydrochloride increases the solubility of folded and unfolded recombinant plasminogen activator rPA. Protein Sci (2010) 0.84

Crystal structure of RNase A tandem enzymes and their interaction with the cytosolic ribonuclease inhibitor. FEBS J (2010) 0.83

Comparative label-free monitoring of immunotoxin efficacy in 2D and 3D mamma carcinoma in vitro models by impedance spectroscopy. Biosens Bioelectron (2013) 0.83

Intracellular uptake and inhibitory activity of aromatic fluorinated amino acids in human breast cancer cells. ChemMedChem (2008) 0.82

A novel fusion protein system for the production of native human pepsinogen in the bacterial periplasm. Protein Expr Purif (2006) 0.82

Periplasmic production of native human proinsulin as a fusion to E. coli ecotin. Protein Expr Purif (2007) 0.82

Recombinant human hyaluronidase Hyal-1: insect cells versus Escherichia coli as expression system and identification of low molecular weight inhibitors. Glycobiology (2007) 0.82

RNAi-mediated suppression of constitutive pulmonary gene expression by small interfering RNA in mice. Pulm Pharmacol Ther (2010) 0.81

A tetrapeptide fragment-based design method results in highly stable artificial proteins. Proteins (2007) 0.81

Oxidative folding of nerve growth factor can be mediated by the pro-peptide of neurotrophin-3. FEBS Lett (2007) 0.81