Localized transfection on arrays of magnetic beads coated with PCR products.

PubWeight™: 1.06‹?› | Rank: Top 15%

🔗 View Article (PMC 2666273)

Published in Nat Methods on January 21, 2005

Authors

Mark Isalan1, Maria Isabel Santori, Cayetano Gonzalez, Luis Serrano

Author Affiliations

1: EMBL, Meyerhofstrasse 1, Heidelberg D-69117, Germany. isalan@embl.de

Articles citing this

Evolvability and hierarchy in rewired bacterial gene networks. Nature (2008) 3.43

Enhancement of the efficiency of non-viral gene delivery by application of pulsed magnetic field. Nucleic Acids Res (2006) 1.01

Cloning-free regulated monitoring of reporter and gene expression. BMC Mol Biol (2009) 0.95

Bioluminescence imaging for assessment and normalization in transfected cell arrays. Biotechnol Bioeng (2007) 0.94

Genetically encoded sender-receiver system in 3D mammalian cell culture. ACS Synth Biol (2013) 0.90

Addition of ascorbic acid to the extracellular environment activates lipoplexes of a ferrocenyl lipid and promotes cell transfection. J Control Release (2011) 0.88

Automated solid-phase subcloning based on beads brought into proximity by magnetic force. PLoS One (2012) 0.86

The living microarray: a high-throughput platform for measuring transcription dynamics in single cells. BMC Genomics (2011) 0.84

Directly transforming PCR-amplified DNA fragments into plant cells is a versatile system that facilitates the transient expression assay. PLoS One (2013) 0.83

Incorporation of DOPE into Lipoplexes formed from a Ferrocenyl Lipid leads to Inverse Hexagonal Nanostructures that allow Redox-Based Control of Transfection in High Serum. Soft Matter (2012) 0.83

Site-specific gene transfer with high efficiency onto a carbon nanotube-loaded electrode. J R Soc Interface (2008) 0.78

Solid-phase cloning for high-throughput assembly of single and multiple DNA parts. Nucleic Acids Res (2015) 0.78

Spatial control of cell transfection using soluble or solid-phase redox agents and a redox-active ferrocenyl lipid. ACS Appl Mater Interfaces (2013) 0.78

Articles cited by this

Initial sequencing and analysis of the human genome. Nature (2001) 212.86

Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A (1988) 193.60

A comprehensive analysis of protein-protein interactions in Saccharomyces cerevisiae. Nature (2000) 47.15

Systematic functional analysis of the Caenorhabditis elegans genome using RNAi. Nature (2003) 26.58

FACS-optimized mutants of the green fluorescent protein (GFP). Gene (1996) 24.12

Predicting subcellular localization of proteins based on their N-terminal amino acid sequence. J Mol Biol (2000) 22.77

A short amino acid sequence able to specify nuclear location. Cell (1984) 21.17

Zinc finger-DNA recognition: crystal structure of a Zif268-DNA complex at 2.1 A. Science (1991) 15.97

A C-terminal signal prevents secretion of luminal ER proteins. Cell (1987) 14.83

Engineered gene circuits. Nature (2002) 6.25

Combinatorial synthesis of genetic networks. Science (2002) 5.91

Microarrays of cells expressing defined cDNAs. Nature (2001) 5.05

The genome of a motile marine Synechococcus. Nature (2003) 4.83

Identification of a peroxisomal targeting signal at the carboxy terminus of firefly luciferase. J Cell Biol (1987) 3.47

GFP-like chromoproteins as a source of far-red fluorescent proteins. FEBS Lett (2001) 3.44

A rapid, generally applicable method to engineer zinc fingers illustrated by targeting the HIV-1 promoter. Nat Biotechnol (2001) 3.12

Elucidating the folding problem of alpha-helices: local motifs, long-range electrostatics, ionic-strength dependence and prediction of NMR parameters. J Mol Biol (1998) 2.78

Zinc fingers are sticking together. Trends Biochem Sci (1998) 2.63

Cleavage site determinants in the mammalian polyadenylation signal. Nucleic Acids Res (1995) 2.63

A microscope-based screening platform for large-scale functional protein analysis in intact cells. FEBS Lett (2003) 2.04

NLSdb: database of nuclear localization signals. Nucleic Acids Res (2003) 1.81

RNAi microarray analysis in cultured mammalian cells. Genome Res (2003) 1.51

RNA interference microarrays: high-throughput loss-of-function genetics in mammalian cells. Proc Natl Acad Sci U S A (2004) 1.29

Cell-biological applications of transfected-cell microarrays. Trends Cell Biol (2002) 1.28

Mammalian two-hybrid system: a complementary approach to the yeast two-hybrid system. Biotechniques (1997) 1.13

Applications of transfected cell microarrays in high-throughput drug discovery. Drug Discov Today (2002) 1.09

siRNA cell arrays for high-content screening microscopy. Biotechniques (2004) 0.90

Motif trap: a rapid method to clone motifs that can target proteins to defined subcellular localisations. J Cell Sci (1999) 0.88

Robotic high-throughput assay for isolating apoptosis-inducing genes. Biotechniques (2002) 0.83

Peroxisomal targeting of mammalian hydroxyacid oxidase 1 requires the C-terminal tripeptide SKI. J Cell Sci (2001) 0.77

Articles by these authors

Predicting changes in the stability of proteins and protein complexes: a study of more than 1000 mutations. J Mol Biol (2002) 9.74

Structure-based redesign of the dimerization interface reduces the toxicity of zinc-finger nucleases. Nat Biotechnol (2007) 8.88

Prediction of sequence-dependent and mutational effects on the aggregation of peptides and proteins. Nat Biotechnol (2004) 7.02

The FoldX web server: an online force field. Nucleic Acids Res (2005) 6.30

Transcriptome complexity in a genome-reduced bacterium. Science (2009) 4.64

Correlation of mRNA and protein in complex biological samples. FEBS Lett (2009) 4.38

Induction of tumor growth by altered stem-cell asymmetric division in Drosophila melanogaster. Nat Genet (2005) 3.99

Proteome organization in a genome-reduced bacterium. Science (2009) 3.97

Impact of genome reduction on bacterial metabolism and its regulation. Science (2009) 3.45

Evolvability and hierarchy in rewired bacterial gene networks. Nature (2008) 3.43

Systematic discovery of new recognition peptides mediating protein interaction networks. PLoS Biol (2005) 3.28

Ectopic expression of germline genes drives malignant brain tumor growth in Drosophila. Science (2010) 3.21

Structure-based assembly of protein complexes in yeast. Science (2004) 2.89

Exploring the sequence determinants of amyloid structure using position-specific scoring matrices. Nat Methods (2010) 2.74

From in vivo to in silico biology and back. Nature (2006) 2.74

Sequence determinants of amyloid fibril formation. Proc Natl Acad Sci U S A (2003) 2.71

SNPeffect: a database mapping molecular phenotypic effects of human non-synonymous coding SNPs. Nucleic Acids Res (2005) 2.69

Noise in transcription negative feedback loops: simulation and experimental analysis. Mol Syst Biol (2006) 2.69

De novo designed peptide-based amyloid fibrils. Proc Natl Acad Sci U S A (2002) 2.59

Control of Drosophila gastrulation by apical localization of adherens junctions and RhoGEF2. Science (2007) 2.51

How protein stability and new functions trade off. PLoS Comput Biol (2008) 2.41

Molecular basis of xeroderma pigmentosum group C DNA recognition by engineered meganucleases. Nature (2008) 2.37

Prediction of water and metal binding sites and their affinities by using the Fold-X force field. Proc Natl Acad Sci U S A (2005) 2.31

Quantification of mRNA and protein and integration with protein turnover in a bacterium. Mol Syst Biol (2011) 2.31

The stability effects of protein mutations appear to be universally distributed. J Mol Biol (2007) 2.25

Protein aggregation and amyloidosis: confusion of the kinds? Curr Opin Struct Biol (2006) 2.10

A comparative study of the relationship between protein structure and beta-aggregation in globular and intrinsically disordered proteins. J Mol Biol (2004) 2.05

Engineering of large numbers of highly specific homing endonucleases that induce recombination on novel DNA targets. J Mol Biol (2005) 2.04

Analysis and description of HOLTIN service provision for AECG monitoring in complex indoor environments. Sensors (Basel) (2013) 2.02

Asterless is a centriolar protein required for centrosome function and embryo development in Drosophila. Curr Biol (2007) 1.99

Protein folding: defining a "standard" set of experimental conditions and a preliminary kinetic data set of two-state proteins. Protein Sci (2005) 1.91

How evolutionary pressure against protein aggregation shaped chaperone specificity. J Mol Biol (2005) 1.91

Computer-aided design of a PDZ domain to recognize new target sequences. Nat Struct Biol (2002) 1.89

Short amino acid stretches can mediate amyloid formation in globular proteins: the Src homology 3 (SH3) case. Proc Natl Acad Sci U S A (2004) 1.83

Recognizing and defining true Ras binding domains I: biochemical analysis. J Mol Biol (2005) 1.76

Computer design of obligate heterodimer meganucleases allows efficient cutting of custom DNA sequences. Nucleic Acids Res (2008) 1.74

Analysis of the human E2 ubiquitin conjugating enzyme protein interaction network. Genome Res (2009) 1.68

Specificity and evolvability in eukaryotic protein interaction networks. PLoS Comput Biol (2006) 1.64

Centrosome dysfunction in Drosophila neural stem cells causes tumors that are not due to genome instability. Curr Biol (2008) 1.64

Polyhomeotic has a tumor suppressor activity mediated by repression of Notch signaling. Nat Genet (2009) 1.62

Drosophila neuroblasts retain the daughter centrosome. Nat Commun (2011) 1.59

Strand-specific deep sequencing of the transcriptome. Genome Res (2010) 1.58

A graphical interface for the FoldX forcefield. Bioinformatics (2011) 1.50

Engineering gene networks to emulate Drosophila embryonic pattern formation. PLoS Biol (2005) 1.48

Contribution of noncentrosomal microtubules to spindle assembly in Drosophila spermatocytes. PLoS Biol (2004) 1.48

Designed tumor necrosis factor-related apoptosis-inducing ligand variants initiating apoptosis exclusively via the DR5 receptor. Proc Natl Acad Sci U S A (2006) 1.45

Cross-talk between phosphorylation and lysine acetylation in a genome-reduced bacterium. Mol Syst Biol (2012) 1.44

A detailed thermodynamic analysis of ras/effector complex interfaces. J Mol Biol (2004) 1.42

The amyloid stretch hypothesis: recruiting proteins toward the dark side. Proc Natl Acad Sci U S A (2005) 1.41

Energy estimation in protein design. Curr Opin Struct Biol (2002) 1.39

Bacterial transcriptomics: what is beyond the RNA horiz-ome? Nat Rev Microbiol (2011) 1.39

PepX: a structural database of non-redundant protein-peptide complexes. Nucleic Acids Res (2009) 1.35

Towards cellular systems in 4D. Cell (2005) 1.33

Recognizing and defining true Ras binding domains II: in silico prediction based on homology modelling and energy calculations. J Mol Biol (2005) 1.33

Comprehensive methylome characterization of Mycoplasma genitalium and Mycoplasma pneumoniae at single-base resolution. PLoS Genet (2013) 1.32

Analyzing protein interaction networks using structural information. Annu Rev Biochem (2008) 1.32

Design of a wearable device for ECG continuous monitoring using wireless technology. Conf Proc IEEE Eng Med Biol Soc (2004) 1.28

Calculation of mutational free energy changes in transition states for protein folding. Biophys J (2003) 1.27

Space as the final frontier in stochastic simulations of biological systems. FEBS Lett (2005) 1.25

Predicted effects of missense mutations on native-state stability account for phenotypic outcome in phenylketonuria, a paradigm of misfolding diseases. Am J Hum Genet (2007) 1.24

Engineering signal transduction pathways. Cell (2010) 1.21

Computational design of peptide ligands. Trends Biotechnol (2011) 1.19

Molecular basis of engineered meganuclease targeting of the endogenous human RAG1 locus. Nucleic Acids Res (2010) 1.19

The interphase microtubule aster is a determinant of asymmetric division orientation in Drosophila neuroblasts. J Cell Biol (2010) 1.17

Unspecific hydrophobic stabilization of folding transition states. Proc Natl Acad Sci U S A (2002) 1.16

Using protein design algorithms to understand the molecular basis of disease caused by protein-DNA interactions: the Pax6 example. Nucleic Acids Res (2010) 1.16

Using a structural and logics systems approach to infer bHLH-DNA binding specificity determinants. Nucleic Acids Res (2011) 1.15

Computer modelling in combination with in vitro studies reveals similar binding affinities of Drosophila Crumbs for the PDZ domains of Stardust and DmPar-6. Eur J Cell Biol (2006) 1.15

Comparative genomics and disorder prediction identify biologically relevant SH3 protein interactions. PLoS Comput Biol (2005) 1.15

Advancing cell biology through proteomics in space and time (PROSPECTS). Mol Cell Proteomics (2012) 1.15

The multiple-specificity landscape of modular peptide recognition domains. Mol Syst Biol (2011) 1.14

An ana2/ctp/mud complex regulates spindle orientation in Drosophila neuroblasts. Dev Cell (2011) 1.13

Cell type-specific importance of ras-c-raf complex association rate constants for MAPK signaling. Sci Signal (2009) 1.12

Personalized respiratory medicine: exploring the horizon, addressing the issues. Summary of a BRN-AJRCCM workshop held in Barcelona on June 12, 2014. Am J Respir Crit Care Med (2015) 1.12

Structures in systems biology. Curr Opin Struct Biol (2007) 1.11

Biased segregation of DNA and centrosomes: moving together or drifting apart? Nat Rev Mol Cell Biol (2009) 1.11

Analysis of disease-linked rhodopsin mutations based on structure, function, and protein stability calculations. J Mol Biol (2010) 1.10

Human epidermal stem cell function is regulated by circadian oscillations. Cell Stem Cell (2013) 1.09

The ubiquitin domain superfold: structure-based sequence alignments and characterization of binding epitopes. J Mol Biol (2005) 1.08

Conformational strain in the hydrophobic core and its implications for protein folding and design. Nat Struct Biol (2002) 1.06

Quantifying information transfer by protein domains: analysis of the Fyn SH2 domain structure. BMC Struct Biol (2008) 1.05

Transcription start site associated RNAs in bacteria. Mol Syst Biol (2012) 1.04

Sequence dependence of amyloid fibril formation: insights from molecular dynamics simulations. J Mol Biol (2005) 1.04

Strategies for protein synthetic biology. Nucleic Acids Res (2010) 1.04

Reconstruction of protein backbones from the BriX collection of canonical protein fragments. PLoS Comput Biol (2008) 1.04

Protein-peptide interactions adopt the same structural motifs as monomeric protein folds. Structure (2009) 1.04

Genome-wide prediction of SH2 domain targets using structural information and the FoldX algorithm. PLoS Comput Biol (2008) 1.02

Crystal structure of I-DmoI in complex with its target DNA provides new insights into meganuclease engineering. Proc Natl Acad Sci U S A (2008) 1.01

High frequency of germline succinate dehydrogenase mutations in sporadic cervical paragangliomas in northern Spain: mitochondrial succinate dehydrogenase structure-function relationships and clinical-pathological correlations. J Clin Endocrinol Metab (2007) 1.01

DR4-selective tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) variants obtained by structure-based design. J Biol Chem (2008) 1.01

Connecting cancer to the asymmetric division of stem cells. Cell (2006) 1.00

Insights into the origin of the tendency of the PI3-SH3 domain to form amyloid fibrils. J Mol Biol (2002) 1.00

BriX: a database of protein building blocks for structural analysis, modeling and design. Nucleic Acids Res (2010) 0.98

Drosophila dd4 mutants reveal that gammaTuRC is required to maintain juxtaposed half spindles in spermatocytes. J Cell Sci (2003) 0.98

Spindle alignment is achieved without rotation after the first cell cycle in Drosophila embryonic neuroblasts. Development (2009) 0.98

The tryptophan switch: changing ligand-binding specificity from type I to type II in SH3 domains. J Mol Biol (2004) 0.96

Structural and functional protein network analyses predict novel signaling functions for rhodopsin. Mol Syst Biol (2011) 0.95

Dissecting the energy metabolism in Mycoplasma pneumoniae through genome-scale metabolic modeling. Mol Syst Biol (2013) 0.95

Amyloid toxicity is independent of polypeptide sequence, length and chirality. J Mol Biol (2007) 0.95