Published in Methods Enzymol on January 01, 1979
Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries. Proc Natl Acad Sci U S A (1985) 13.19
Cloning and expression of the Mycobacterium bovis BCG gene for extracellular alpha antigen. J Bacteriol (1988) 3.97
Isolation of cDNA clones for the human complement protein factor B, a class III major histocompatibility complex gene product. Proc Natl Acad Sci U S A (1982) 3.89
Isolation and sequence of cDNA clones coding for a member of the family of high mobility group proteins (HMG-T) in trout and analysis of HMG-T-mRNA's in trout tissues. Nucleic Acids Res (1985) 1.91
Isolation and characterization of cDNA clones for human apolipoprotein A-I. Proc Natl Acad Sci U S A (1982) 1.66
Single-base-pair discrimination of terminal mismatches by using oligonucleotide microarrays and neural network analyses. Appl Environ Microbiol (2002) 1.48
A human and a plant intron-containing tRNATyr gene are both transcribed in a HeLa cell extract but spliced along different pathways. EMBO J (1987) 1.47
Development and application of oligonucleotide probes for molecular identification of Xenorhabdus species. Appl Environ Microbiol (1990) 1.34
Adjacent upstream activation sequence elements synergistically regulate transcription of ADH2 in Saccharomyces cerevisiae. Mol Cell Biol (1989) 1.32
All human tRNATyr genes contain introns as a prerequisite for pseudouridine biosynthesis in the anticodon. Nucleic Acids Res (1988) 1.20
Use of synthetic oligonucleotide probes complementary to genes for human HLA-DR alpha and beta as extension primers for the isolation of 5'-specific genomic clones. Proc Natl Acad Sci U S A (1983) 1.09
Estimation of bacterial cell numbers in humic acid-rich salt marsh sediments with probes directed to 16S ribosomal DNA Appl Environ Microbiol (1999) 0.94
Characterization of the gene encoding the hemocyanin subunit e from the tarantula Eurypelma californicum. Proc Natl Acad Sci U S A (1990) 0.90
The double-strand-break repair model for recombination. Cell (1983) 31.21
In vitro selection of RNA molecules that bind specific ligands. Nature (1990) 30.19
Getting started with yeast. Methods Enzymol (1991) 26.67
Yeast transformation: a model system for the study of recombination. Proc Natl Acad Sci U S A (1981) 26.54
Terminal labeling and addition of homopolymer tracts to duplex DNA fragments by terminal deoxynucleotidyl transferase. Nucleic Acids Res (1976) 23.88
DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mapping. Nucleic Acids Res (1974) 19.42
DNA sequence required for efficient transcription termination in yeast. Cell (1982) 14.19
Mutagenesis by insertion of a drug-resistance element carrying an inverted repetition. J Mol Biol (1975) 11.14
Identification and purification of natural killer cell stimulatory factor (NKSF), a cytokine with multiple biologic effects on human lymphocytes. J Exp Med (1989) 10.60
Genetic applications of yeast transformation with linear and gapped plasmids. Methods Enzymol (1983) 8.96
Arylsulfonyltetrazoles, new coupling reagents and further improvements in the triester method for the synthesis of deoxyribooligonucleotides. Nucleic Acids Res (1977) 8.92
A mutant with a defect in telomere elongation leads to senescence in yeast. Cell (1989) 8.69
Elution of DNA from agarose gels after electrophoresis. Methods Enzymol (1979) 8.36
Double-strand breaks at an initiation site for meiotic gene conversion. Nature (1989) 7.82
Isolation and characterization of rat and human glyceraldehyde-3-phosphate dehydrogenase cDNAs: genomic complexity and molecular evolution of the gene. Nucleic Acids Res (1985) 7.67
Amino acid replacements resulting from super-suppression of nonsense mutants of iso-1-cytochrome c from yeast. J Mol Biol (1971) 7.19
Extensive 3'-overhanging, single-stranded DNA associated with the meiosis-specific double-strand breaks at the ARG4 recombination initiation site. Cell (1991) 6.80
In vitro selection of functional nucleic acids. Annu Rev Biochem (1999) 6.55
DNA sequences of telomeres maintained in yeast. Nature (1984) 6.52
Organization of DNA sequences and replication origins at yeast telomeres. Cell (1983) 6.43
Cloning yeast telomeres on linear plasmid vectors. Cell (1982) 6.39
Yeast recombination: the association between double-strand gap repair and crossing-over. Proc Natl Acad Sci U S A (1983) 6.10
Isolation of new ribozymes from a large pool of random sequences [see comment]. Science (1993) 6.08
An initiation site for meiotic gene conversion in the yeast Saccharomyces cerevisiae. Nature (1989) 5.99
Nucleotide sequence analysis of DNA. II. Complete nucleotide sequence of the cohesive ends of bacteriophage lambda DNA. J Mol Biol (1971) 5.80
Terminal labeling and addition of homopolymer tracts to duplex DNA fragments by terminal deoxynucleotidyl transferase. Nucleic Acids Res (1976) 5.73
BK virus DNA: complete nucleotide sequence of a human tumor virus. Science (1979) 5.61
Selection of lys2 Mutants of the Yeast SACCHAROMYCES CEREVISIAE by the Utilization of alpha-AMINOADIPATE. Genetics (1979) 5.41
Uracil incorporation: a source of pulse-labeled DNA fragments in the replication of the Escherichia coli chromosome. Proc Natl Acad Sci U S A (1978) 5.30
Mating signals control expression of mutations resulting from insertion of a transposable repetitive element adjacent to diverse yeast genes. Cell (1980) 5.20
Unequal crossing over in the ribosomal DNA of Saccharomyces cerevisiae. Nature (1980) 5.12
RNA-peptide fusions for the in vitro selection of peptides and proteins. Proc Natl Acad Sci U S A (1997) 5.06
Fungal recombination. Microbiol Rev (1985) 4.90
Transient accumulation of Okazaki fragments as a result of uracil incorporation into nascent DNA. Proc Natl Acad Sci U S A (1977) 4.83
Mutants of yeast defective in iso-1-cytochrome c. Genetics (1974) 4.73
Portable microcomputer software for nucleotide sequence analysis. Nucleic Acids Res (1982) 4.72
Excision repair of uracil incorporated in DNA as a result of a defect in dUTPase. J Mol Biol (1977) 4.68
Pedigree analysis of plasmid segregation in yeast. Cell (1983) 4.65
The guanosine binding site of the Tetrahymena ribozyme. Nature (1989) 4.43
HIV-1 Rev regulation involves recognition of non-Watson-Crick base pairs in viral RNA. Cell (1991) 4.43
Mutants of S. cerevisiae defective in the maintenance of minichromosomes. Genetics (1984) 4.39
Mutations in PTEN are frequent in endometrial carcinoma but rare in other common gynecological malignancies. Cancer Res (1997) 4.32
Insertion of a genetic marker into the ribosomal DNA of yeast. Plasmid (1979) 4.20
Saccharomyces cerevisiae protein involved in plasmid maintenance is necessary for mating of MAT alpha cells. J Mol Biol (1988) 4.15
Construction of artificial chromosomes in yeast. Nature (1983) 4.10
Identification and characterization of genes and mutants for an N-terminal acetyltransferase from yeast. EMBO J (1989) 4.08
An RNA motif that binds ATP. Nature (1993) 3.86
Exonuclease III: use for DNA sequence analysis and in specific deletions of nucleotides. Methods Enzymol (1983) 3.65
Genes affecting the expression of cytochrome c in yeast: genetic mapping and genetic interactions. Genetics (1980) 3.63
A reliable mapping method for sequence determination of oligodeoxyribonucleotides by mobility shift analysis. Anal Biochem (1976) 3.56
Unusual DNA sequences associated with the ends of yeast chromosomes. Nature (1984) 3.54
An improved procedure for utilizing terminal transferase to add homopolymers to the 3' termini of DNA. Nucleic Acids Res (1981) 3.53
Specificity and frequency of ultraviolet-induced reversion of an iso-1-cytochrome c ochre mutant in radiation-sensitive strains of yeast. J Mol Biol (1974) 3.50
A deletion map of cyc1 mutants and its correspondence to mutationally altered iso-1-cytochromes c of yeast. Genetics (1975) 3.48
Terminal transferase-catalyzed addition of nucleotides to the 3' termini of DNA. Methods Enzymol (1980) 3.44
The mutational alteration of the primary structure of yeast iso-1-cytochrome c. J Biol Chem (1968) 3.42
A protein involved in minichromosome maintenance in yeast binds a transcriptional enhancer conserved in eukaryotes. Genes Dev (1989) 3.41
A DNA aptamer that binds adenosine and ATP. Biochemistry (1995) 3.40
Transfer of yeast telomeres to linear plasmids by recombination. Cell (1984) 3.39
Genetics and biosynthesis of cytochrome c. Annu Rev Genet (1971) 3.38
Mutants of yeast initiating translation of iso-1-cytochrome c within a region spanning 37 nucleotides. Cell (1980) 3.37
Activation of mammalian Chk1 during DNA replication arrest: a role for Chk1 in the intra-S phase checkpoint monitoring replication origin firing. J Cell Biol (2001) 3.35
Carbohydrate-deficient glycoprotein syndrome type Ib. Phosphomannose isomerase deficiency and mannose therapy. J Clin Invest (1998) 3.32
The ARD1 gene of yeast functions in the switch between the mitotic cell cycle and alternative developmental pathways. Cell (1985) 3.28
Micromanipulation and dissection of asci. Methods Enzymol (1991) 3.24
Telomeric repeat from T. thermophila cross hybridizes with human telomeres. Nature (1988) 3.20
Chromosomal rearrangements associated with morphological mutants provide a means for genetic variation of Candida albicans. J Bacteriol (1990) 3.17
Chromosome segregation in mitosis and meiosis. Annu Rev Cell Biol (1985) 3.14
Amino-terminal processing of mutant forms of yeast iso-1-cytochrome c. The specificities of methionine aminopeptidase and acetyltransferase. J Biol Chem (1985) 3.13
Structural conventions for group I introns. Nucleic Acids Res (1987) 3.12
Mcm2 is a target of regulation by Cdc7-Dbf4 during the initiation of DNA synthesis. Genes Dev (1997) 3.05
Autonomously replicating sequences in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A (1980) 3.04
An improved strategy for rapid direct sequencing of both strands of long DNA molecules cloned in a plasmid. Nucleic Acids Res (1983) 3.02
A general method for inserting specific DNA sequences into cloning vehicles. Gene (1976) 3.02
Selection in vitro of single-stranded DNA molecules that fold into specific ligand-binding structures. Nature (1992) 3.02
Cell cycle-regulated nuclear localization of MCM2 and MCM3, which are required for the initiation of DNA synthesis at chromosomal replication origins in yeast. Genes Dev (1993) 2.99
Serine substitutions caused by an ochre suppressor in yeast. J Mol Biol (1975) 2.98
Isolation of an efficient actin promoter for use in rice transformation. Plant Cell (1990) 2.94
Incidence and risk factors for side effects of spinal anesthesia. Anesthesiology (1992) 2.94
Mcm2 and Mcm3, two proteins important for ARS activity, are related in structure and function. Genes Dev (1991) 2.92
A mutation allowing an mRNA secondary structure diminishes translation of Saccharomyces cerevisiae iso-1-cytochrome c. Mol Cell Biol (1985) 2.91
Selection of invasive and metastatic subpopulations from a human lung adenocarcinoma cell line. Am J Respir Cell Mol Biol (1997) 2.89
Mutagenic specificity: reversion of iso-1-cytochrome c mutants of yeast. J Mol Biol (1973) 2.88
New rapid methods for DNA sequencing based in exonuclease III digestion followed by repair synthesis. Nucleic Acids Res (1982) 2.88
Functional proteins from a random-sequence library. Nature (2001) 2.81
Chemical synthesis and sequence studies of deoxyribooligonucleotides which constitute the duplex sequence of the lactose operator of Escherichia coli. J Biol Chem (1975) 2.79
Transformation of yeast with synthetic oligonucleotides. Proc Natl Acad Sci U S A (1988) 2.77
Role of the calcium-independent transient outward current I(to1) in shaping action potential morphology and duration. Circ Res (2000) 2.75
Chromosome length controls mitotic chromosome segregation in yeast. Cell (1986) 2.73
Physical interactions among Mcm proteins and effects of Mcm dosage on DNA replication in Saccharomyces cerevisiae. Mol Cell Biol (1996) 2.72
Packaging of an oversize transducing genome by Salmonella phage P22. J Mol Biol (1974) 2.71
Initiating DNA synthesis: from recruiting to activating the MCM complex. J Cell Sci (2001) 2.66
Phenotypic suppression of nonsense mutants in yeast by aminoglycoside antibiotics. Nature (1979) 2.66
Mapping and gene conversion studies with the structural gene for iso-1-cytochrome C in yeast. Genetics (1975) 2.65
Specific Saccharomyces cerevisiae genes are expressed in response to DNA-damaging agents. Mol Cell Biol (1985) 2.63
The use of mRNA display to select high-affinity protein-binding peptides. Proc Natl Acad Sci U S A (2001) 2.63
Nucleotide sequence analysis of DNA. XII. The chemical synthesis and sequence analysis of a dodecadeoxynucleotide which binds to the endolysin gene of bacteriophage lambda. Biochem Biophys Res Commun (1973) 2.63
Heart and combined heart-kidney transplantation in patients with concomitant renal insufficiency and end-stage heart failure. Am J Transplant (2013) 2.61