Published in Biochemistry on March 07, 2006
Biomolecular electrostatics and solvation: a computational perspective. Q Rev Biophys (2012) 1.39
Engineering protein allostery: 1.05 A resolution structure and enzymatic properties of a Na+-activated trypsin. J Mol Biol (2008) 0.83
Functional and structural characterization of Vibrio cholerae extracellular serine protease B, VesB. J Biol Chem (2014) 0.80
Why Ser and not Thr brokers catalysis in the trypsin fold. Biochemistry (2015) 0.80
Combinatorial enzyme design probes allostery and cooperativity in the trypsin fold. J Mol Biol (2010) 0.79
Molecular Basis of Enhanced Activity in Factor VIIa-Trypsin Variants Conveys Insights into Tissue Factor-mediated Allosteric Regulation of Factor VIIa Activity. J Biol Chem (2015) 0.75
Severe FVII deficiency caused by a new point mutation combined with a previously undetected gene deletion. Br J Haematol (2005) 2.00
Evolution of enzyme cascades from embryonic development to blood coagulation. Trends Biochem Sci (2002) 1.96
A C60 primary ion beam system for time of flight secondary ion mass spectrometry: its development and secondary ion yield characteristics. Anal Chem (2003) 1.96
Thrombin mutant W215A/E217A acts as a platelet GPIb antagonist. Arterioscler Thromb Vasc Biol (2007) 1.80
Role of Na+ and K+ in enzyme function. Physiol Rev (2006) 1.80
Molecular dissection of Na+ binding to thrombin. J Biol Chem (2004) 1.72
The development of inflammatory joint disease is attenuated in mice expressing the anticoagulant prothrombin mutant W215A/E217A. Blood (2011) 1.71
Studies on the basis for the properties of fibrin produced from fibrinogen-containing gamma' chains. Blood (2005) 1.64
Protective signaling by activated protein C is mechanistically linked to protein C activation on endothelial cells. J Biol Chem (2006) 1.58
Exposure of R169 controls protein C activation and autoactivation. Blood (2012) 1.52
Conformational selection or induced fit? A critical appraisal of the kinetic mechanism. Biochemistry (2012) 1.39
Structural identification of the pathway of long-range communication in an allosteric enzyme. Proc Natl Acad Sci U S A (2008) 1.31
Rapid kinetics of Na+ binding to thrombin. J Biol Chem (2006) 1.29
Expression, purification, and characterization of authentic monoferric and apo-human serum transferrins. Protein Expr Purif (2004) 1.25
Investigation of the mechanism of iron release from the C-lobe of human serum transferrin: mutational analysis of the role of a pH sensitive triad. Biochemistry (2003) 1.23
Effect of glycosylation on the function of a soluble, recombinant form of the transferrin receptor. Biochemistry (2006) 1.23
Crystal structures of murine thrombin in complex with the extracellular fragments of murine protease-activated receptors PAR3 and PAR4. Proc Natl Acad Sci U S A (2007) 1.23
Conformational selection in trypsin-like proteases. Curr Opin Struct Biol (2012) 1.20
Properties and mechanisms of action of naturally occurring antifungal peptides. Cell Mol Life Sci (2013) 1.20
The thrombin mutant W215A/E217A shows safe and potent anticoagulant and antithrombotic effects in vivo. J Biol Chem (2002) 1.19
Evolution of peptidase diversity. J Biol Chem (2008) 1.16
Regulation of the fibrosis and angiogenesis promoter SPARC/osteonectin in human adipose tissue by weight change, leptin, insulin, and glucose. Diabetes (2009) 1.13
Intrinsic fluorescence reports a global conformational change in the N-lobe of human serum transferrin following iron release. Biochemistry (2007) 1.13
Relative antithrombotic and antihemostatic effects of protein C activator versus low-molecular-weight heparin in primates. Blood (2007) 1.12
Differential effect of a his tag at the N- and C-termini: functional studies with recombinant human serum transferrin. Biochemistry (2002) 1.12
Crystal structure of thrombin in a self-inhibited conformation. J Biol Chem (2006) 1.11
The anticoagulant thrombin mutant W215A/E217A has a collapsed primary specificity pocket. J Biol Chem (2004) 1.10
Mutational analysis of C-lobe ligands of human serum transferrin: insights into the mechanism of iron release. Biochemistry (2005) 1.09
Engineering thrombin for selective specificity toward protein C and PAR1. J Biol Chem (2010) 1.09
Thrombomodulin changes the molecular surface of interaction and the rate of complex formation between thrombin and protein C. J Biol Chem (2004) 1.08
Synergistic anion and metal binding to the ferric ion-binding protein from Neisseria gonorrhoeae. J Biol Chem (2002) 1.08
Allostery in trypsin-like proteases suggests new therapeutic strategies. Trends Biotechnol (2011) 1.07
Redesigning the monovalent cation specificity of an enzyme. Proc Natl Acad Sci U S A (2003) 1.05
Conformational selection is a dominant mechanism of ligand binding. Biochemistry (2013) 1.05
Evidence that His349 acts as a pH-inducible switch to accelerate receptor-mediated iron release from the C-lobe of human transferrin. J Biol Inorg Chem (2010) 1.03
Mechanism of Na(+) binding to thrombin resolved by ultra-rapid kinetics. Biophys Chem (2007) 1.01
A fully human, allosteric monoclonal antibody that activates the insulin receptor and improves glycemic control. Diabetes (2012) 1.01
Crystal structure of thrombin in complex with fibrinogen gamma' peptide. Biophys Chem (2006) 0.99
Crystallographic and kinetic evidence of allostery in a trypsin-like protease. Biochemistry (2011) 0.98
Mutant N143P reveals how Na+ activates thrombin. J Biol Chem (2009) 0.97
Crystal structures of prethrombin-2 reveal alternative conformations under identical solution conditions and the mechanism of zymogen activation. Biochemistry (2011) 0.97
Important role of the cys-191 cys-220 disulfide bond in thrombin function and allostery. J Biol Chem (2007) 0.96
A novel, potent dual inhibitor of the leukocyte proteases cathepsin G and chymase: molecular mechanisms and anti-inflammatory activity in vivo. J Biol Chem (2005) 0.96
Crystal structure of thrombin bound to the uncleaved extracellular fragment of PAR1. J Biol Chem (2010) 0.96
Crystal structure of prethrombin-1. Proc Natl Acad Sci U S A (2010) 0.96
Crystal structure of the anticoagulant slow form of thrombin. J Biol Chem (2002) 0.96
Composition of pH-sensitive triad in C-lobe of human serum transferrin. Comparison to sequences of ovotransferrin and lactoferrin provides insight into functional differences in iron release. Biochemistry (2005) 0.96
Dissecting substrate recognition by thrombin using the inactive mutant S195A. Biophys Chem (2003) 0.96
Energetic and structural consequences of perturbing Gly-193 in the oxyanion hole of serine proteases. J Biol Chem (2005) 0.95
Activation of stress signalling pathways enhances tolerance of fungi to chemical fungicides and antifungal proteins. Cell Mol Life Sci (2014) 0.95
A loop in the N-lobe of human serum transferrin is critical for binding to the transferrin receptor as revealed by mutagenesis, isothermal titration calorimetry, and epitope mapping. J Mol Recognit (2009) 0.95
Genetically engineering transferrin to improve its in vitro ability to deliver cytotoxins. J Control Release (2008) 0.95
Mechanism of the anticoagulant activity of thrombin mutant W215A/E217A. J Biol Chem (2009) 0.95
Thrombin mutant W215A/E217A treatment improves neurological outcome and reduces cerebral infarct size in a mouse model of ischemic stroke. Stroke (2011) 0.94
Inequivalent contribution of the five tryptophan residues in the C-lobe of human serum transferrin to the fluorescence increase when iron is released. Biochemistry (2009) 0.94
The conformation of the activation peptide of protein C is influenced by Ca2+ and Na+ binding. J Biol Chem (2004) 0.93
Murine thrombin lacks Na+ activation but retains high catalytic activity. J Biol Chem (2006) 0.93
Recombinant expression and functional characterization of human hephaestin: a multicopper oxidase with ferroxidase activity. Biochemistry (2005) 0.93
Ser(214) is crucial for substrate binding to serine proteases. J Biol Chem (2002) 0.92
Discovery of potent, selective, orally active, nonpeptide inhibitors of human mast cell chymase. J Med Chem (2007) 0.92
Human hephaestin expression is not limited to enterocytes of the gastrointestinal tract but is also found in the antrum, the enteric nervous system, and pancreatic {beta}-cells. Am J Physiol Gastrointest Liver Physiol (2009) 0.92
Autoactivation of thrombin precursors. J Biol Chem (2013) 0.92
Crystal structure of prothrombin reveals conformational flexibility and mechanism of activation. J Biol Chem (2013) 0.92
Three-dimensional models of proteases involved in patterning of the Drosophila Embryo. Crucial role of predicted cation binding sites. J Biol Chem (2002) 0.92
Identification and mechanism of action of the plant defensin NaD1 as a new member of the antifungal drug arsenal against Candida albicans. Antimicrob Agents Chemother (2013) 0.92
Stabilization of the E* form turns thrombin into an anticoagulant. J Biol Chem (2009) 0.91
Multi-copper oxidases and human iron metabolism. Nutrients (2013) 0.90
The thrombin epitope recognizing thrombomodulin is a highly cooperative hot spot in exosite I. J Biol Chem (2002) 0.90
Residue Asp-189 controls both substrate binding and the monovalent cation specificity of thrombin. J Biol Chem (2003) 0.89
Evidence of the E*-E equilibrium from rapid kinetics of Na+ binding to activated protein C and factor Xa. J Phys Chem B (2010) 0.89
Hirudin binding reveals key determinants of thrombin allostery. J Biol Chem (2005) 0.88
Rigidification of the autolysis loop enhances Na(+) binding to thrombin. Biophys Chem (2011) 0.88
High resolution crystal structures of free thrombin in the presence of K(+) reveal the molecular basis of monovalent cation selectivity and an inactive slow form. Biophys Chem (2006) 0.88
The position of arginine 124 controls the rate of iron release from the N-lobe of human serum transferrin. A structural study. J Biol Chem (2002) 0.87
Preclinical characterization of Aurora kinase inhibitor R763/AS703569 identified through an image-based phenotypic screen. J Cancer Res Clin Oncol (2010) 0.86
Thrombin functions through its RGD sequence in a non-canonical conformation. J Biol Chem (2005) 0.86
Conserved Ser residues, the shutter region, and speciation in serpin evolution. J Biol Chem (2003) 0.86
Maximizing filamentous phage yield during computer-controlled fermentation. Bioprocess Biosyst Eng (2009) 0.86
Histone H4 promotes prothrombin autoactivation. J Biol Chem (2013) 0.86
Substrate recognition drives the evolution of serine proteases. J Biol Chem (2002) 0.85
Structural basis of Na+ activation mimicry in murine thrombin. J Biol Chem (2007) 0.85
Human RBP4 adipose tissue expression is gender specific and influenced by leptin. Clin Endocrinol (Oxf) (2011) 0.85
Structural basis of thrombin-protease-activated receptor interactions. IUBMB Life (2011) 0.84
Engineering protein allostery: 1.05 A resolution structure and enzymatic properties of a Na+-activated trypsin. J Mol Biol (2008) 0.83
Cytochrome b(5) is a major reductant in vivo of human indoleamine 2,3-dioxygenase expressed in yeast. FEBS Lett (2006) 0.83
Structure-based mutagenesis reveals critical residues in the transferrin receptor participating in the mechanism of pH-induced release of iron from human serum transferrin. Biochemistry (2012) 0.83
Three-dimensional modeling of thrombin-fibrinogen interaction. J Biol Chem (2002) 0.83
High density array screening to identify the genetic requirements for transition metal tolerance in Saccharomyces cerevisiae. Metallomics (2011) 0.82
Identification of the epitope of a monoclonal antibody that disrupts binding of human transferrin to the human transferrin receptor. FEBS J (2005) 0.82
Engineering protease specificity made simple, but not simpler. Nat Chem Biol (2008) 0.82
Redesigning allosteric activation in an enzyme. Proc Natl Acad Sci U S A (2011) 0.82
Engineering the primary substrate specificity of Streptomyces griseus trypsin. Biochemistry (2003) 0.82
Properties of a homogeneous C-lobe prepared by introduction of a TEV cleavage site between the lobes of human transferrin. Protein Expr Purif (2010) 0.81
Ionic residues of human serum transferrin affect binding to the transferrin receptor and iron release. Biochemistry (2012) 0.81
Structural and functional consequences of the substitution of glycine 65 with arginine in the N-lobe of human transferrin. Biochemistry (2009) 0.81
A novel murine protein with no effect on iron homoeostasis is homologous with transferrin and is the putative inhibitor of carbonic anhydrase. Biochem J (2007) 0.80
Why Ser and not Thr brokers catalysis in the trypsin fold. Biochemistry (2015) 0.80
The role of Zn2+ on the structure and stability of murine adenosine deaminase. J Phys Chem B (2010) 0.79
Combinatorial enzyme design probes allostery and cooperativity in the trypsin fold. J Mol Biol (2010) 0.79
Is Na+ a coagulation factor? Thromb Haemost (2006) 0.79