Published in Proc Natl Acad Sci U S A on September 01, 1986
Multiple cis regulatory elements for maximal expression of the cauliflower mosaic virus 35S promoter in transgenic plants. Plant Cell (1989) 3.54
The CaMV 35S enhancer contains at least two domains which can confer different developmental and tissue-specific expression patterns. EMBO J (1989) 2.98
Isolation of an efficient actin promoter for use in rice transformation. Plant Cell (1990) 2.94
Electroporation by using bipolar oscillating electric field: an improved method for DNA transfection of NIH 3T3 cells. Proc Natl Acad Sci U S A (1991) 1.98
Evaluation of selectable markers for obtaining stable transformants in the gramineae. Plant Physiol (1988) 1.40
Expression of mRNA electroporated into plant and animal cells. Nucleic Acids Res (1987) 1.27
Study of mechanisms of electric field-induced DNA transfection. III. Electric parameters and other conditions for effective transfection. Biophys J (1992) 1.12
Autonomous transposition of the tobacco retrotransposon Tto1 in rice. Plant Cell (1996) 1.11
Molecular genetic improvement of cereals: transgenic wheat (Triticum aestivum L.). Plant Cell Rep (2007) 1.01
Soybean protoplast culture and direct gene uptake and expression by cultured soybean protoplasts. Plant Physiol (1987) 0.92
Wheat nuclear protein HBP-1 binds to the hexameric sequence in the promoter of various plant genes. Nucleic Acids Res (1989) 0.89
Uptake and expression of bacterial and cyanobacterial genes by isolated cucumber etioplasts. Proc Natl Acad Sci U S A (1987) 0.89
Electroporation: an arsenal of application. Cytotechnology (2007) 0.82
Transfection by electroporation. Curr Protoc Mol Biol (2003) 0.77
Genetic transformation of Sorghum bicolor. Physiol Mol Biol Plants (2009) 0.76
A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology (1973) 127.36
Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Mol Cell Biol (1982) 116.75
A simple and general method for transferring genes into plants. Science (1985) 25.43
Gene transfer into mouse lyoma cells by electroporation in high electric fields. EMBO J (1982) 17.98
Enhancer-dependent expression of human kappa immunoglobulin genes introduced into mouse pre-B lymphocytes by electroporation. Proc Natl Acad Sci U S A (1984) 17.51
Nucleotide sequence analysis of the chloramphenicol resistance transposon Tn9. Nature (1980) 12.45
Expression of genes transferred into monocot and dicot plant cells by electroporation. Proc Natl Acad Sci U S A (1985) 12.21
Direct transfer of cloned genes from bacteria to mammalian cells. Proc Natl Acad Sci U S A (1980) 6.21
Stable transformation of maize after gene transfer by electroporation. Nature (1986) 5.40
Transient expression of genes introduced into cultured cells of Drosophila. Proc Natl Acad Sci U S A (1983) 3.93
Electric field mediated gene transfer. Biochem Biophys Res Commun (1982) 3.91
Bacterial gene inserted in an engineered RNA virus: efficient expression in monocotyledonous plant cells. Science (1986) 3.09
Cauliflower mosaic virus on its way to becoming a useful plant vector. Curr Top Microbiol Immunol (1982) 2.22
The 5' termini of RNAs encoded by the transposable element copia. Nucleic Acids Res (1981) 1.89
Construction of a heat-inducible gene for plants. Demonstration of heat-inducible activity of the Drosophila hsp70 promoter in plants. EMBO J (1985) 1.33
Use of carboxyfluorescein diacetate to study formation of permeable channels between mouse blastomeres. Nature (1982) 1.17
Evolution of cytochrome C investigated by the maximum parsimony method. J Mol Evol (1981) 1.13
Human hybridoma cells produced by electro-fusion. FEBS Lett (1982) 0.88
Terminal labeling and addition of homopolymer tracts to duplex DNA fragments by terminal deoxynucleotidyl transferase. Nucleic Acids Res (1976) 23.88
DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mapping. Nucleic Acids Res (1974) 19.42
Arylsulfonyltetrazoles, new coupling reagents and further improvements in the triester method for the synthesis of deoxyribooligonucleotides. Nucleic Acids Res (1977) 8.92
Elution of DNA from agarose gels after electrophoresis. Methods Enzymol (1979) 8.36
Isolation and characterization of rat and human glyceraldehyde-3-phosphate dehydrogenase cDNAs: genomic complexity and molecular evolution of the gene. Nucleic Acids Res (1985) 7.67
Nucleotide sequence analysis of DNA. II. Complete nucleotide sequence of the cohesive ends of bacteriophage lambda DNA. J Mol Biol (1971) 5.80
Terminal labeling and addition of homopolymer tracts to duplex DNA fragments by terminal deoxynucleotidyl transferase. Nucleic Acids Res (1976) 5.73
BK virus DNA: complete nucleotide sequence of a human tumor virus. Science (1979) 5.61
Unequal crossing over in the ribosomal DNA of Saccharomyces cerevisiae. Nature (1980) 5.12
Portable microcomputer software for nucleotide sequence analysis. Nucleic Acids Res (1982) 4.72
Mutations in PTEN are frequent in endometrial carcinoma but rare in other common gynecological malignancies. Cancer Res (1997) 4.32
Insertion of a genetic marker into the ribosomal DNA of yeast. Plasmid (1979) 4.20
Exonuclease III: use for DNA sequence analysis and in specific deletions of nucleotides. Methods Enzymol (1983) 3.65
A reliable mapping method for sequence determination of oligodeoxyribonucleotides by mobility shift analysis. Anal Biochem (1976) 3.56
An improved procedure for utilizing terminal transferase to add homopolymers to the 3' termini of DNA. Nucleic Acids Res (1981) 3.53
Terminal transferase-catalyzed addition of nucleotides to the 3' termini of DNA. Methods Enzymol (1980) 3.44
Activation of mammalian Chk1 during DNA replication arrest: a role for Chk1 in the intra-S phase checkpoint monitoring replication origin firing. J Cell Biol (2001) 3.35
Carbohydrate-deficient glycoprotein syndrome type Ib. Phosphomannose isomerase deficiency and mannose therapy. J Clin Invest (1998) 3.32
An improved strategy for rapid direct sequencing of both strands of long DNA molecules cloned in a plasmid. Nucleic Acids Res (1983) 3.02
A general method for inserting specific DNA sequences into cloning vehicles. Gene (1976) 3.02
Isolation of an efficient actin promoter for use in rice transformation. Plant Cell (1990) 2.94
Incidence and risk factors for side effects of spinal anesthesia. Anesthesiology (1992) 2.94
Selection of invasive and metastatic subpopulations from a human lung adenocarcinoma cell line. Am J Respir Cell Mol Biol (1997) 2.89
New rapid methods for DNA sequencing based in exonuclease III digestion followed by repair synthesis. Nucleic Acids Res (1982) 2.88
Chemical synthesis and sequence studies of deoxyribooligonucleotides which constitute the duplex sequence of the lactose operator of Escherichia coli. J Biol Chem (1975) 2.79
Role of the calcium-independent transient outward current I(to1) in shaping action potential morphology and duration. Circ Res (2000) 2.75
Nucleotide sequence analysis of DNA. XII. The chemical synthesis and sequence analysis of a dodecadeoxynucleotide which binds to the endolysin gene of bacteriophage lambda. Biochem Biophys Res Commun (1973) 2.63
Cloned synthetic lac operator DNA is biologically active. Nature (1976) 2.59
Sequence analysis of short DNA fragments. Methods Enzymol (1980) 2.56
Mild and moderate asthma is associated with airway goblet cell hyperplasia and abnormalities in mucin gene expression. Am J Respir Crit Care Med (2001) 2.53
IEX-1L, an apoptosis inhibitor involved in NF-kappaB-mediated cell survival. Science (1998) 2.46
Control of oligodendrocyte differentiation by the Nkx2.2 homeodomain transcription factor. Development (2001) 2.43
Terminal transferase: use of the tailing of DNA and for in vitro mutagenesis. Methods Enzymol (1983) 2.36
Molecular genetic evidence supporting the clonality and appendiceal origin of Pseudomyxoma peritonei in women. Am J Pathol (1999) 2.34
The interleukin 1 inhibitor rilonacept in treatment of chronic gouty arthritis: results of a placebo-controlled, monosequence crossover, non-randomised, single-blind pilot study. Ann Rheum Dis (2009) 2.29
Rice chloroplast DNA molecules are heterogeneous as revealed by DNA sequences of a cluster of genes. Nucleic Acids Res (1987) 2.25
Mapping the 5'-terminal nucleotides of the DNA of bacteriophage lambda and related phages. Proc Natl Acad Sci U S A (1967) 2.23
Differential regulation of airway mucin gene expression and mucin secretion by extracellular nucleotide triphosphates. Am J Respir Cell Mol Biol (2001) 2.22
Synchronous digestion of SV40 DNA by exonuclease III. Biochemistry (1976) 2.21
Rice alcohol dehydrogenase genes: anaerobic induction, organ specific expression and characterization of cDNA clones. Plant Mol Biol (1989) 2.20
The growth of cells in serum-free hormone-supplemented media. Methods Enzymol (1979) 2.18
Hybridization with synthetic oligonucleotides. Methods Enzymol (1979) 2.17
Synthetic adaptors for cloning DNA. Methods Enzymol (1979) 2.14
Nucleotide sequence analysis of bacteriophage DNA. Methods Enzymol (1974) 2.14
Microsatellite instability and the clinicopathological features of sporadic colorectal cancer. Gut (2001) 2.08
Nucleotide sequence analysis of DNA. Nat New Biol (1972) 2.07
Nucleotide sequence analysis of DNA. IX. Use of oligonucleotides of defined sequence as primers in DNA sequence analysis. Biochem Biophys Res Commun (1972) 2.04
Minor vein structure and sugar transport in Arabidopsis thaliana. Planta (2000) 1.98
Minimal length of the lactose operator sequence for the specific recognition by the lactose repressor. Proc Natl Acad Sci U S A (1977) 1.95
Complete nucleotide sequence of the cohesive ends of bacteriophage P2 deoxyribonucleic acid. J Biol Chem (1974) 1.94
MR nerve imaging in a prospective cohort of patients with suspected carpal tunnel syndrome. Neurology (2002) 1.94
Gender-related differences in the characteristics of problem gamblers using a gambling helpline. Am J Psychiatry (2001) 1.93
DNA sequence of a mutation in the leader region of the yeast iso-1-cytochrome c mRNA. Cell (1981) 1.92
Nucleotide sequence analysis of DNA. IV. Complete nucleotide sequence of the left-hand cohesive end of coliphage 186 DNA. J Mol Biol (1972) 1.88
Growth and differentiation of human nasal epithelial cells in culture. Serum-free, hormone-supplemented medium and proteoglycan synthesis. Am Rev Respir Dis (1985) 1.87
Nucleotide sequence analysis of DNA. I. Partial sequence of the cohesive ends of bacteriophage lambda and 186 DNA. J Mol Biol (1970) 1.86
Profiling expression patterns and isolating differentially expressed genes by cDNA microarray system with colorimetry detection. Genomics (1998) 1.84
Isolation and structure of a rat cytochrome c gene. J Biol Chem (1981) 1.81
Changes in pulmonary function test results after 1 year of therapy as predictors of survival in patients with idiopathic pulmonary fibrosis. Chest (1995) 1.78
Can physicians be induced to resume obstetric practice? J Am Board Fam Pract (1992) 1.78
Nonallelic members of the cytochrome c multigene family of the rat may arise through different messenger RNAs. Cell (1983) 1.76
Structure and base sequence in the cohesive ends of bacteriophage lambda DNA. J Mol Biol (1968) 1.76
RNA polymerase pausing and transcript release at the lambda tR1 terminator in vitro. J Biol Chem (1983) 1.71
Analysis of rice Act1 5' region activity in transgenic rice plants. Plant Cell (1991) 1.70
High-velocity microprojectiles for delivering nucleic acids into living cells. 1987. Biotechnology (1992) 1.69
Global analysis of gene expression in invasion by a lung cancer model. Cancer Res (2001) 1.65
Synthesis of human insulin gene. VIII. Construction of expression vectors for fused proinsulin production in Escherichia coli. Gene (1984) 1.64
BK virus DNA sequence coding for the amino-terminus of the T-antigen. Virology (1979) 1.62
Interaction of a gibberellin-induced factor with the upstream region of an alpha-amylase gene in rice aleurone tissue. Proc Natl Acad Sci U S A (1988) 1.58
Characterization of human mucin 5B gene expression in airway epithelium and the genomic clone of the amino-terminal and 5'-flanking region. Am J Respir Cell Mol Biol (2001) 1.56
Nucleotide sequence analysis of deoxyribonucleic acid. VII. Characterization of Escherichia coli exonuclease 3 activity for possible use in terminal nucleotide sequence analysis of duplex deoxyribonucleic acid. J Biol Chem (1972) 1.56
Identification of phloem involved in assimilate loading in leaves by the activity of the galactinol synthase promoter. Plant Physiol (2000) 1.55
Exome sequencing-driven discovery of coding polymorphisms associated with common metabolic phenotypes. Diabetologia (2012) 1.55
Comparative study of papovavirus DNA: BKV(MM), BKV(WT) and SV40. Nucleic Acids Res (1979) 1.55
Cytochrome oxidase subunit II gene of rice has an insertion sequence within the intron. Nucleic Acids Res (1984) 1.53
Synthesis of the human insulin gene. Part III. Chemical synthesis of 5'-phosphomonoester group containing deoxyribooligonucleotides by the modified phosphotriester method. Its application in the synthesis of seventeen fragments constituting human insulin C-chain DNA. Nucleic Acids Res (1980) 1.53
A biphasic chamber system for maintaining polarity of differentiation of cultured respiratory tract epithelial cells. In Vitro Cell Dev Biol (1988) 1.50
Nucleotide sequence analysis of DNA. XI. The 3' terminal sequences of bacteriophage lambda and phi 80 DNA. Biochem Biophys Res Commun (1973) 1.50
Construction of expression vectors based on the rice actin 1 (Act1) 5' region for use in monocot transformation. Mol Gen Genet (1991) 1.49
Nucleotide recognition sequence at the cleavage site of Haemophilus aegyptius II (Hae II) restriction endonuclease. Biochem Biophys Res Commun (1976) 1.48
Chemical and enzymatic synthesis of lactose operator of Escherichia coli and its binding to lactose repressor. Proc Natl Acad Sci U S A (1976) 1.48
Risk factors for subclinical atherosclerosis in a prospective cohort of patients with systemic lupus erythematosus. Ann Rheum Dis (2003) 1.48
Structure of two unlinked Drosophila melanogaster glyceraldehyde-3-phosphate dehydrogenase genes. J Biol Chem (1985) 1.48
Control of glycolysis by phosphofructokinase in slices of rat liver, Novikoff hepatoma, and adenocarcinomas. Biochem Biophys Res Commun (1964) 1.47
Continuous multiplication of rabbit tracheal epithelial cells in a defined, hormone-supplemented medium. In Vitro (1982) 1.46
Induction of T-cell activation by oxidized low density lipoprotein. Arterioscler Thromb (1992) 1.46
Chemical synthesis of versatile adaptors for molecular cloning. Biochem Biophys Res Commun (1978) 1.45
Loss of FHIT expression in cervical carcinoma cell lines and primary tumors. Cancer Res (1997) 1.42
Transcription terminates at lambda tR1 in three clusters. Proc Natl Acad Sci U S A (1982) 1.42
A simple and rapid nucleotide sequencing strategy and its application in analyzing a rice histone 3 gene. Gene (1986) 1.40
Adaptors, linkers, and methylation. Methods Enzymol (1987) 1.39
BK virus DNA sequence: extent of homology with simian virus 40 DNA. Proc Natl Acad Sci U S A (1979) 1.39
Retracted Nucleotide sequences of mitochondrial ATPase subunit 9 genes from three lines of rice (Oryza sativa L.) Nucleic Acids Res (1990) 1.39
Recognition sequence of restriction endonuclease KpnI from Klebsiella pneumoniae. Nucleic Acids Res (1978) 1.38
Nucleotide sequence analysis of DNA. X. Complete nucleotide sequence of the cohesive ends of bacteriophage phi80 DNA. J Mol Biol (1973) 1.38
TP53 mutations are frequent in malignant NF1 tumors. Genes Chromosomes Cancer (1994) 1.37
Light-regulated and cell-specific expression of tomato rbcS-gusA and rice rbcS-gusA fusion genes in transgenic rice. Plant Physiol (1993) 1.37
The role of replication proteins in the regulation of bacteriophage T4 transcription. II. Gene 45 and late transcription uncoupled from replication. J Mol Biol (1975) 1.35
The geminivirus BR1 movement protein binds single-stranded DNA and localizes to the cell nucleus. Plant Cell (1994) 1.34