Mutations in the Yersinia pseudotuberculosis type III secretion system needle protein, YscF, that specifically abrogate effector translocation into host cells.

PubWeight™: 1.16‹?› | Rank: Top 10%

🔗 View Article (PMC 1797200)

Published in J Bacteriol on October 27, 2006

Authors

Alison J Davis1, Joan Mecsas

Author Affiliations

1: Department of Molecular Biology and Microbiology, Tufts University School of Medicine, 136 Harrison Ave., Boston, MA 02111, USA.

Articles citing this

Protein export according to schedule: architecture, assembly, and regulation of type III secretion systems from plant- and animal-pathogenic bacteria. Microbiol Mol Biol Rev (2012) 1.92

The Shigella T3SS needle transmits a signal for MxiC release, which controls secretion of effectors. Mol Microbiol (2010) 1.65

What's the point of the type III secretion system needle? Proc Natl Acad Sci U S A (2008) 1.58

Bacterial type III secretion systems: specialized nanomachines for protein delivery into target cells. Annu Rev Microbiol (2014) 1.55

Innate immune recognition of Yersinia pseudotuberculosis type III secretion. PLoS Pathog (2009) 1.38

YscP and YscU switch the substrate specificity of the Yersinia type III secretion system by regulating export of the inner rod protein YscI. J Bacteriol (2008) 1.32

Structural characterization of the Yersinia pestis type III secretion system needle protein YscF in complex with its heterodimeric chaperone YscE/YscG. J Mol Biol (2008) 1.26

The presence of professional phagocytes dictates the number of host cells targeted for Yop translocation during infection. Cell Microbiol (2010) 1.20

Control of effector export by the Pseudomonas aeruginosa type III secretion proteins PcrG and PcrV. Mol Microbiol (2010) 1.17

Bioinformatic and biochemical evidence for the identification of the type III secretion system needle protein of Chlamydia trachomatis. J Bacteriol (2007) 1.12

Identification and characterization of small-molecule inhibitors of Yop translocation in Yersinia pseudotuberculosis. Antimicrob Agents Chemother (2010) 1.09

Small molecule inhibitors of LcrF, a Yersinia pseudotuberculosis transcription factor, attenuate virulence and limit infection in a murine pneumonia model. Infect Immun (2010) 1.06

Multi-Functional Characteristics of the Pseudomonas aeruginosa Type III Needle-Tip Protein, PcrV; Comparison to Orthologs in other Gram-negative Bacteria. Front Microbiol (2011) 1.03

Impassable YscP substrates and their impact on the Yersinia enterocolitica type III secretion pathway. J Bacteriol (2008) 1.01

The common structural architecture of Shigella flexneri and Salmonella typhimurium type three secretion needles. PLoS Pathog (2013) 0.98

Impact of host membrane pore formation by the Yersinia pseudotuberculosis type III secretion system on the macrophage innate immune response. Infect Immun (2013) 0.92

YopK controls both rate and fidelity of Yop translocation. Mol Microbiol (2012) 0.90

Modified needle-tip PcrV proteins reveal distinct phenotypes relevant to the control of type III secretion and intoxication by Pseudomonas aeruginosa. PLoS One (2011) 0.89

Yersinia pseudotuberculosis uses Ail and YadA to circumvent neutrophils by directing Yop translocation during lung infection. Cell Microbiol (2013) 0.89

ROS-inhibitory activity of YopE is required for full virulence of Yersinia in mice. Cell Microbiol (2010) 0.88

The pathogenesis, detection, and prevention of Vibrio parahaemolyticus. Front Microbiol (2015) 0.87

Yersinia pseudotuberculosis virulence determinants invasin, YopE, and YopT modulate RhoG activity and localization. Infect Immun (2009) 0.87

A dominant-negative needle mutant blocks type III secretion of early but not late substrates in Yersinia. Mol Microbiol (2010) 0.86

YopR impacts type III needle polymerization in Yersinia species. Mol Microbiol (2009) 0.83

A mutant with aberrant extracellular LcrV-YscF interactions fails to form pores and translocate Yop effector proteins but retains the ability to trigger Yop secretion in response to host cell contact. J Bacteriol (2013) 0.82

RfaL is required for Yersinia pestis type III secretion and virulence. Infect Immun (2013) 0.81

Mutated and bacteriophage T4 nanoparticle arrayed F1-V immunogens from Yersinia pestis as next generation plague vaccines. PLoS Pathog (2013) 0.81

Generation and Characterization of a scFv Antibody Against T3SS Needle of Vibrio parahaemolyticus. Indian J Microbiol (2013) 0.78

The type III secretion system apparatus determines the intracellular niche of bacterial pathogens. Proc Natl Acad Sci U S A (2016) 0.77

Piericidin A1 Blocks Yersinia Ysc Type III Secretion System Needle Assembly. mSphere (2017) 0.75

Kinase Activities of RIPK1 and RIPK3 Can Direct IFN-β Synthesis Induced by Lipopolysaccharide. J Immunol (2017) 0.75

Articles cited by this

Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (1970) 1528.65

Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J Mol Biol (1986) 55.34

A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR. J Bacteriol (1988) 31.85

Construction of an eae deletion mutant of enteropathogenic Escherichia coli by using a positive-selection suicide vector. Infect Immun (1991) 15.39

Multiplication of Shigella flexneri within HeLa cells: lysis of the phagocytic vacuole and plasmid-mediated contact hemolysis. Infect Immun (1986) 9.35

Tightly regulated tac promoter vectors useful for the expression of unfused and fused proteins in Escherichia coli. Gene (1988) 8.81

Supramolecular structure of the Salmonella typhimurium type III protein secretion system. Science (1998) 6.55

Target cell contact triggers expression and polarized transfer of Yersinia YopE cytotoxin into mammalian cells. EMBO J (1994) 6.37

Translocation of a hybrid YopE-adenylate cyclase from Yersinia enterocolitica into HeLa cells. Mol Microbiol (1994) 5.82

The cytotoxic protein YopE of Yersinia obstructs the primary host defence. Mol Microbiol (1990) 5.09

A novel EspA-associated surface organelle of enteropathogenic Escherichia coli involved in protein translocation into epithelial cells. EMBO J (1998) 4.53

Intracellular targeting of the Yersinia YopE cytotoxin in mammalian cells induces actin microfilament disruption. Infect Immun (1991) 4.46

The YopB protein of Yersinia pseudotuberculosis is essential for the translocation of Yop effector proteins across the target cell plasma membrane and displays a contact-dependent membrane disrupting activity. EMBO J (1996) 3.82

The tripartite type III secreton of Shigella flexneri inserts IpaB and IpaC into host membranes. J Cell Biol (1999) 3.75

The surface-located YopN protein is involved in calcium signal transduction in Yersinia pseudotuberculosis. Mol Microbiol (1991) 3.56

Structure and composition of the Shigella flexneri "needle complex", a part of its type III secreton. Mol Microbiol (2001) 3.46

The invasion-associated type III system of Salmonella typhimurium directs the translocation of Sip proteins into the host cell. Mol Microbiol (1997) 3.42

Molecular characterization and assembly of the needle complex of the Salmonella typhimurium type III protein secretion system. Proc Natl Acad Sci U S A (2000) 3.38

Analysis of the V antigen lcrGVH-yopBD operon of Yersinia pseudotuberculosis: evidence for a regulatory role of LcrH and LcrV. J Bacteriol (1991) 3.32

Process of protein transport by the type III secretion system. Microbiol Mol Biol Rev (2004) 3.31

An Inv/Mxi-Spa-like type III protein secretion system in Burkholderia pseudomallei modulates intracellular behaviour of the pathogen. Mol Microbiol (2002) 3.23

Contribution of Salmonella typhimurium type III secretion components to needle complex formation. Proc Natl Acad Sci U S A (2000) 3.10

Cell-surface-bound Yersinia translocate the protein tyrosine phosphatase YopH by a polarized mechanism into the target cell. Mol Microbiol (1995) 3.06

The V-antigen of Yersinia forms a distinct structure at the tip of injectisome needles. Science (2005) 2.90

Status of YopM and YopN in the Yersinia Yop virulon: YopM of Y.enterocolitica is internalized inside the cytosol of PU5-1.8 macrophages by the YopB, D, N delivery apparatus. EMBO J (1996) 2.61

The structure of Yersinia pestis V-antigen, an essential virulence factor and mediator of immunity against plague. Structure (2004) 2.53

Isolation and characterization of Ca2+-blind mutants of Yersinia pestis. J Bacteriol (1985) 2.49

Type III protein secretion mechanism in mammalian and plant pathogens. Biochim Biophys Acta (2004) 2.44

Supramolecular structure of the Shigella type III secretion machinery: the needle part is changeable in length and essential for delivery of effectors. EMBO J (2000) 2.39

The V-antigen of Yersinia is surface exposed before target cell contact and involved in virulence protein translocation. Mol Microbiol (1999) 2.37

The needle length of bacterial injectisomes is determined by a molecular ruler. Science (2003) 2.27

The various and varying roles of specific chaperones in type III secretion systems. Curr Opin Microbiol (2003) 2.25

Supermolecular structure of the enteropathogenic Escherichia coli type III secretion system and its direct interaction with the EspA-sheath-like structure. Proc Natl Acad Sci U S A (2001) 2.21

Molecular model of a type III secretion system needle: Implications for host-cell sensing. Proc Natl Acad Sci U S A (2006) 2.16

Assembly of the inner rod determines needle length in the type III secretion injectisome. Nature (2006) 2.15

Helical structure of the needle of the type III secretion system of Shigella flexneri. J Biol Chem (2003) 2.15

LcrG, a secreted protein involved in negative regulation of the low-calcium response in Yersinia pestis. J Bacteriol (1993) 2.08

Insertion of a Yop translocation pore into the macrophage plasma membrane by Yersinia enterocolitica: requirement for translocators YopB and YopD, but not LcrG. Mol Microbiol (1999) 2.08

Differential effects of deletions in lcrV on secretion of V antigen, regulation of the low-Ca2+ response, and virulence of Yersinia pestis. J Bacteriol (1995) 1.93

The needle component of the type III secreton of Shigella regulates the activity of the secretion apparatus. J Biol Chem (2005) 1.92

Bacterial injectisomes: needle length does matter. Science (2005) 1.86

Virulence role of V antigen of Yersinia pestis at the bacterial surface. Infect Immun (1999) 1.80

TyeA, a protein involved in control of Yop release and in translocation of Yersinia Yop effectors. EMBO J (1998) 1.78

Polymerization of a single protein of the pathogen Yersinia enterocolitica into needles punctures eukaryotic cells. Proc Natl Acad Sci U S A (2001) 1.75

LcrV, a substrate for Yersinia enterocolitica type III secretion, is required for toxin targeting into the cytosol of HeLa cells. J Biol Chem (2000) 1.75

Identification of attenuated Yersinia pseudotuberculosis strains and characterization of an orogastric infection in BALB/c mice on day 5 postinfection by signature-tagged mutagenesis. Infect Immun (2001) 1.75

Detection of pathogenic Yersinia enterocolitica by using congo red-magnesium oxalate agar medium. J Clin Microbiol (1989) 1.74

IpaD of Shigella flexneri is independently required for regulation of Ipa protein secretion and efficient insertion of IpaB and IpaC into host membranes. Infect Immun (2005) 1.73

Novel gyrA point mutation in a strain of Escherichia coli resistant to fluoroquinolones but not to nalidixic acid. Antimicrob Agents Chemother (1993) 1.73

Yersinia pestis LcrV forms a stable complex with LcrG and may have a secretion-related regulatory role in the low-Ca2+ response. J Bacteriol (1997) 1.71

Yersinia enterocolitica type III secretion-translocation system: channel formation by secreted Yops. EMBO J (1999) 1.68

Protective anti-V antibodies inhibit Pseudomonas and Yersinia translocon assembly within host membranes. J Infect Dis (2005) 1.67

The Yersinia pestis type III secretion needle plays a role in the regulation of Yop secretion. Mol Microbiol (2005) 1.66

Requirement of the Yersinia pseudotuberculosis effectors YopH and YopE in colonization and persistence in intestinal and lymph tissues. Infect Immun (2003) 1.64

The V antigen of Yersinia pestis regulates Yop vectorial targeting as well as Yop secretion through effects on YopB and LcrG. J Bacteriol (1998) 1.60

LcrV is a channel size-determining component of the Yop effector translocon of Yersinia. Mol Microbiol (2001) 1.56

Solution structure of monomeric BsaL, the type III secretion needle protein of Burkholderia pseudomallei. J Mol Biol (2006) 1.55

Type III machines of pathogenic yersiniae secrete virulence factors into the extracellular milieu. Mol Microbiol (1999) 1.55

Chaperones of the type III secretion pathway: jacks of all trades. Mol Microbiol (2002) 1.52

Role of EscF, a putative needle complex protein, in the type III protein translocation system of enteropathogenic Escherichia coli. Cell Microbiol (2001) 1.46

Role of the Hrp pilus in type III protein secretion in Pseudomonas syringae. Science (2001) 1.41

Genetic analysis of the formation of the Ysc-Yop translocation pore in macrophages by Yersinia enterocolitica: role of LcrV, YscF and YopN. Microb Pathog (2003) 1.39

Type III secretion: the bacteria-eukaryotic cell express. FEMS Microbiol Lett (2005) 1.36

Roles of LcrG and LcrV during type III targeting of effector Yops by Yersinia enterocolitica. J Bacteriol (2001) 1.32

YscB of Yersinia pestis functions as a specific chaperone for YopN. J Bacteriol (1998) 1.31

Shigella Spa32 is an essential secretory protein for functional type III secretion machinery and uniformity of its needle length. J Bacteriol (2002) 1.29

Selection and characterization of Yersinia pestis YopN mutants that constitutively block Yop secretion. Mol Microbiol (2005) 1.28

LcrV of Yersinia pestis enters infected eukaryotic cells by a virulence plasmid-independent mechanism. Infect Immun (1999) 1.16

Identification of SycN, YscX, and YscY, three new elements of the Yersinia yop virulon. J Bacteriol (1999) 1.15

Yersinia has a tropism for B and T cell zones of lymph nodes that is independent of the type III secretion system. PLoS Pathog (2006) 1.14

YopB of Yersinia enterocolitica is essential for YopE translocation. Infect Immun (2001) 1.14

Polarity of enteropathogenic Escherichia coli EspA filament assembly and protein secretion. J Bacteriol (2005) 1.09

PscF is a major component of the Pseudomonas aeruginosa type III secretion needle. FEMS Microbiol Lett (2005) 1.05

Two substrate-targeting sites in the Yersinia protein tyrosine phosphatase co-operate to promote bacterial virulence. Mol Microbiol (2005) 1.00

Yersinia pestis Yop secretion protein F: purification, characterization, and protective efficacy against bubonic plague. Protein Expr Purif (2005) 0.96

Comparative analysis of type III effector translocation by Yersinia pseudotuberculosis expressing native LcrV or PcrV from Pseudomonas aeruginosa. J Infect Dis (2003) 0.93

Articles by these authors

Yersinia pseudotuberculosis disseminates directly from a replicating bacterial pool in the intestine. J Exp Med (2006) 2.11

Self-regulation of Candida albicans population size during GI colonization. PLoS Pathog (2007) 1.85

Requirement of the Yersinia pseudotuberculosis effectors YopH and YopE in colonization and persistence in intestinal and lymph tissues. Infect Immun (2003) 1.64

CD8(+) T cells restrict Yersinia pseudotuberculosis infection: bypass of anti-phagocytosis by targeting antigen-presenting cells. PLoS Pathog (2009) 1.24

The presence of professional phagocytes dictates the number of host cells targeted for Yop translocation during infection. Cell Microbiol (2010) 1.20

Yersinia has a tropism for B and T cell zones of lymph nodes that is independent of the type III secretion system. PLoS Pathog (2006) 1.14

The C-terminal tail of Yersinia pseudotuberculosis YopM is critical for interacting with RSK1 and for virulence. Infect Immun (2010) 1.14

Identification and characterization of small-molecule inhibitors of Yop translocation in Yersinia pseudotuberculosis. Antimicrob Agents Chemother (2010) 1.09

Small molecule inhibitors of LcrF, a Yersinia pseudotuberculosis transcription factor, attenuate virulence and limit infection in a murine pneumonia model. Infect Immun (2010) 1.06

Identification of MrtAB, an ABC transporter specifically required for Yersinia pseudotuberculosis to colonize the mesenteric lymph nodes. PLoS Pathog (2012) 1.02

Intranasal inoculation of mice with Yersinia pseudotuberculosis causes a lethal lung infection that is dependent on Yersinia outer proteins and PhoP. Infect Immun (2006) 1.01

Identifying Yersinia YopH-targeted signal transduction pathways that impair neutrophil responses during in vivo murine infection. Cell Host Microbe (2013) 0.97

The proinflammatory response induced by wild-type Yersinia pseudotuberculosis infection inhibits survival of yop mutants in the gastrointestinal tract and Peyer's patches. Infect Immun (2006) 0.95

A non-invasive quantitative assay to measure murine intestinal inflammation using the neutrophil marker lactoferrin. J Immunol Methods (2006) 0.93

Bacterial Secretion Systems: An Overview. Microbiol Spectr (2016) 0.91

Adhesins and host serum factors drive Yop translocation by yersinia into professional phagocytes during animal infection. PLoS Pathog (2013) 0.90

Yersinia pseudotuberculosis uses Ail and YadA to circumvent neutrophils by directing Yop translocation during lung infection. Cell Microbiol (2013) 0.89

ROS-inhibitory activity of YopE is required for full virulence of Yersinia in mice. Cell Microbiol (2010) 0.88

Development of a vaccinia virus based reservoir-targeted vaccine against Yersinia pestis. Vaccine (2010) 0.88

Effect of age on susceptibility to Salmonella Typhimurium infection in C57BL/6 mice. J Med Microbiol (2009) 0.88

Yersinia pestis AcrAB-TolC in antibiotic resistance and virulence. Antimicrob Agents Chemother (2011) 0.86

A dominant-negative needle mutant blocks type III secretion of early but not late substrates in Yersinia. Mol Microbiol (2010) 0.86

Mutations in the Pseudomonas aeruginosa needle protein gene pscF confer resistance to phenoxyacetamide inhibitors of the type III secretion system. Antimicrob Agents Chemother (2014) 0.83

Oral inoculation with Type III secretion mutants of Yersinia pseudotuberculosis provides protection from oral, intraperitoneal, or intranasal challenge with virulent Yersinia. Vaccine (2006) 0.82

A mutant with aberrant extracellular LcrV-YscF interactions fails to form pores and translocate Yop effector proteins but retains the ability to trigger Yop secretion in response to host cell contact. J Bacteriol (2013) 0.82

Effect of MarA-like proteins on antibiotic resistance and virulence in Yersinia pestis. Infect Immun (2009) 0.77