Eg5 steps it up!

The homotetrameric kinesin-5 KLP61F preferentially crosslinks microtubules into antiparallel orientations. Curr Biol (2008) 1.64

Kinesin-5-dependent poleward flux and spindle length control in Drosophila embryo mitosis. Mol Biol Cell (2009) 1.57

Kinesin-5/Eg5 is important for transport of CARTS from the trans-Golgi network to the cell surface. J Cell Biol (2013) 1.47

Phospho-regulated interaction between kinesin-6 Klp9p and microtubule bundler Ase1p promotes spindle elongation. Dev Cell (2009) 1.35

Spindle pole regulation by a discrete Eg5-interacting domain in TPX2. Curr Biol (2008) 1.26

Dynamic partitioning of mitotic kinesin-5 cross-linkers between microtubule-bound and freely diffusing states. J Cell Biol (2008) 1.15

Directionality of individual kinesin-5 Cin8 motors is modulated by loop 8, ionic strength and microtubule geometry. EMBO J (2011) 1.13

Getting in sync with dimeric Eg5. Initiation and regulation of the processive run. J Biol Chem (2007) 1.08

The bipolar assembly domain of the mitotic motor kinesin-5. Nat Commun (2013) 1.03

Structural basis for the assembly of the mitotic motor Kinesin-5 into bipolar tetramers. Elife (2014) 1.01

Microtubule-nucleus interactions in Dictyostelium discoideum mediated by central motor kinesins. Eukaryot Cell (2009) 0.93

Kinesin-5 in Drosophila embryo mitosis: sliding filament or spindle matrix mechanism? Cell Motil Cytoskeleton (2009) 0.91

Tyrosines in the kinesin-5 head domain are necessary for phosphorylation by Wee1 and for mitotic spindle integrity. Curr Biol (2009) 0.91

mRNA expression analysis of cell cycle genes in islets of pregnant mice. Diabetologia (2010) 0.88

Inhibition of KSP by ARRY-520 induces cell cycle block and cell death via the mitochondrial pathway in AML cells. Leukemia (2009) 0.86

Essential genes for astroglial development and axon pathfinding during zebrafish embryogenesis. Dev Dyn (2010) 0.85

Virosome presents multimodel cancer therapy without viral replication. Biomed Res Int (2013) 0.80

Kif11 dependent cell cycle progression in radial glial cells is required for proper neurogenesis in the zebrafish neural tube. Dev Biol (2013) 0.79

Development of hemiasterlin derivatives as potential anticancer agents that inhibit tubulin polymerization and synergize with a stilbene tubulin inhibitor. Invest New Drugs (2011) 0.78

Phase II study to assess the efficacy, safety and tolerability of the mitotic spindle kinesin inhibitor AZD4877 in patients with recurrent advanced urothelial cancer. Invest New Drugs (2013) 0.78

Histone H3 Phosphorylation in Human Skin Histoculture as a Tool to Evaluate Patient's Response to Antiproliferative Drugs. Biomark Insights (2016) 0.75

Bead movement by single kinesin molecules studied with optical tweezers. Nature (1990) 8.23

Kinesin walks hand-over-hand. Science (2003) 7.14

Mitotic spindle organization by a plus-end-directed microtubule motor. Nature (1992) 6.52

Fluorescent speckle microscopy, a method to visualize the dynamics of protein assemblies in living cells. Curr Biol (1998) 5.69

Phosphorylation by p34cdc2 regulates spindle association of human Eg5, a kinesin-related motor essential for bipolar spindle formation in vivo. Cell (1995) 5.68

Mechanics of the kinesin step. Nature (2005) 5.15

The bipolar mitotic kinesin Eg5 moves on both microtubules that it crosslinks. Nature (2005) 4.63

Two mitotic kinesins cooperate to drive sister chromatid separation during anaphase. Nature (2003) 4.63

Crystal structure of the kinesin motor domain reveals a structural similarity to myosin. Nature (1996) 4.48

The depolymerizing kinesin MCAK uses lattice diffusion to rapidly target microtubule ends. Nature (2006) 3.89

Kinesin moves by an asymmetric hand-over-hand mechanism. Science (2003) 3.83

Myosin step size. Estimation from slow sliding movement of actin over low densities of heavy meromyosin. J Mol Biol (1990) 3.80

The spindle: a dynamic assembly of microtubules and motors. Nat Cell Biol (2001) 3.74

The bipolar kinesin, KLP61F, cross-links microtubules within interpolar microtubule bundles of Drosophila embryonic mitotic spindles. J Cell Biol (1999) 3.44

Kinesin-related cut7 protein associates with mitotic and meiotic spindles in fission yeast. Nature (1992) 3.33

The kinesin-like protein KLP61F is essential for mitosis in Drosophila. J Cell Biol (1993) 3.32

Probing the kinesin reaction cycle with a 2D optical force clamp. Proc Natl Acad Sci U S A (2003) 3.25

Crystal structure of the motor domain of the kinesin-related motor ncd. Nature (1996) 3.21

Opposing motor activities are required for the organization of the mammalian mitotic spindle pole. J Cell Biol (1996) 3.15

Poly(ADP-ribose) is required for spindle assembly and structure. Nature (2004) 3.11

Direct observation of microtubule dynamics at kinetochores in Xenopus extract spindles: implications for spindle mechanics. J Cell Biol (2003) 3.01

A mitotic lamin B matrix induced by RanGTP required for spindle assembly. Science (2006) 2.87

Switch-based mechanism of kinesin motors. Nature (2001) 2.86

Poleward microtubule flux mitotic spindles assembled in vitro. J Cell Biol (1991) 2.80

Alternate fast and slow stepping of a heterodimeric kinesin molecule. Nat Cell Biol (2003) 2.74

Individual dimers of the mitotic kinesin motor Eg5 step processively and support substantial loads in vitro. Nat Cell Biol (2006) 2.73

Mechanism of the single-headed processivity: diffusional anchoring between the K-loop of kinesin and the C terminus of tubulin. Proc Natl Acad Sci U S A (2000) 2.73

A processive single-headed motor: kinesin superfamily protein KIF1A. Science (1999) 2.64

Stepping and stretching. How kinesin uses internal strain to walk processively. J Biol Chem (2003) 2.60

A "slow" homotetrameric kinesin-related motor protein purified from Drosophila embryos. J Biol Chem (1994) 2.57

Efficient mitosis in human cells lacking poleward microtubule flux. Curr Biol (2005) 2.39

Kinesin's processivity results from mechanical and chemical coordination between the ATP hydrolysis cycles of the two motor domains. Proc Natl Acad Sci U S A (1999) 2.27

Poleward microtubule flux is a major component of spindle dynamics and anaphase a in mitotic Drosophila embryos. Curr Biol (2002) 2.27

Eg5 is static in bipolar spindles relative to tubulin: evidence for a static spindle matrix. J Cell Biol (2001) 2.23

Crystal structure of the mitotic spindle kinesin Eg5 reveals a novel conformation of the neck-linker. J Biol Chem (2001) 2.18

The kinesin Eg5 drives poleward microtubule flux in Xenopus laevis egg extract spindles. J Cell Biol (2004) 2.06

Cloning by differential screening of a Xenopus cDNA that encodes a kinesin-related protein. Mol Cell Biol (1991) 1.95

Backsteps induced by nucleotide analogs suggest the front head of kinesin is gated by strain. Proc Natl Acad Sci U S A (2006) 1.85

KIF1A alternately uses two loops to bind microtubules. Science (2004) 1.82

Allosteric inhibition of kinesin-5 modulates its processive directional motility. Nat Chem Biol (2006) 1.81

Working strokes by single molecules of the kinesin-related microtubule motor ncd. Nat Cell Biol (2000) 1.71

Monastrol stabilises an attached low-friction mode of Eg5. Curr Biol (2004) 1.58

Spindle microtubules in flux. J Cell Sci (2005) 1.56

NuMA is a component of an insoluble matrix at mitotic spindle poles. Cell Motil Cytoskeleton (1999) 1.45

Mitosis, microtubules, and the matrix. J Cell Biol (2001) 1.43

Kinetic evidence for low chemical processivity in ncd and Eg5. J Mol Biol (1997) 1.39

Nanometres and piconewtons: the macromolecular mechanics of kinesin. Trends Cell Biol (1995) 1.31

Docking and rolling, a model of how the mitotic motor Eg5 works. J Biol Chem (2005) 1.30

A structural model for monastrol inhibition of dimeric kinesin Eg5. EMBO J (2006) 1.27

Kinesin 5-independent poleward flux of kinetochore microtubules in PtK1 cells. J Cell Biol (2006) 1.27

A force balance model of early spindle pole separation in Drosophila embryos. Biophys J (2003) 1.25

A mechanistic model for the organization of microtubule asters by motor and non-motor proteins in a mammalian mitotic extract. Mol Biol Cell (2004) 1.23

Eg5 causes elongation of meiotic spindles when flux-associated microtubule depolymerization is blocked. Curr Biol (2004) 1.19

Monomeric kinesin head domains hydrolyze multiple ATP molecules before release from a microtubule. J Biol Chem (1997) 1.12

Mechanism and function of poleward flux in Xenopus extract meiotic spindles. Philos Trans R Soc Lond B Biol Sci (2005) 1.08

Functional roles of poleward microtubule flux during mitosis. Cell Cycle (2006) 1.05

Motility of dimeric ncd on a metal-chelating surfactant: evidence that ncd is not processive. Biochemistry (1999) 0.96

Searching for a spindle matrix. J Cell Biol (2001) 0.88

Optical trapping. Rev Sci Instrum (2004) 7.15

Direct observation of base-pair stepping by RNA polymerase. Nature (2005) 6.29

Ubiquitous transcriptional pausing is independent of RNA polymerase backtracking. Cell (2003) 3.92

Kinesin moves by an asymmetric hand-over-hand mechanism. Science (2003) 3.83

An automated two-dimensional optical force clamp for single molecule studies. Biophys J (2002) 3.57

Nanomechanical measurements of the sequence-dependent folding landscapes of single nucleic acid hairpins. Proc Natl Acad Sci U S A (2006) 3.56

Sequence-resolved detection of pausing by single RNA polymerase molecules. Cell (2006) 3.50

Backtracking by single RNA polymerase molecules observed at near-base-pair resolution. Nature (2003) 3.50

Direct measurement of the full, sequence-dependent folding landscape of a nucleic acid. Science (2006) 3.49

Direct observation of hierarchical folding in single riboswitch aptamers. Science (2008) 3.22

Passive all-optical force clamp for high-resolution laser trapping. Phys Rev Lett (2005) 3.13

Coordination of opposite-polarity microtubule motors. J Cell Biol (2002) 3.04

Individual dimers of the mitotic kinesin motor Eg5 step processively and support substantial loads in vitro. Nat Cell Biol (2006) 2.73

Stepping and stretching. How kinesin uses internal strain to walk processively. J Biol Chem (2003) 2.60

High-resolution, single-molecule measurements of biomolecular motion. Annu Rev Biophys Biomol Struct (2007) 2.58

Applied force reveals mechanistic and energetic details of transcription termination. Cell (2008) 2.45

Simultaneous, coincident optical trapping and single-molecule fluorescence. Nat Methods (2004) 2.20

Sequence-dependent pausing of single lambda exonuclease molecules. Science (2003) 1.88

Backsteps induced by nucleotide analogs suggest the front head of kinesin is gated by strain. Proc Natl Acad Sci U S A (2006) 1.85

Forward and reverse motion of single RecBCD molecules on DNA. Biophys J (2004) 1.82

Single-molecule studies of RNA polymerase: motoring along. Annu Rev Biochem (2008) 1.80

Single-molecule, motion-based DNA sequencing using RNA polymerase. Science (2006) 1.69

Binding and translocation of termination factor rho studied at the single-molecule level. J Mol Biol (2012) 1.58

Combined optical trapping and single-molecule fluorescence. J Biol (2003) 1.52

Trigger loop dynamics mediate the balance between the transcriptional fidelity and speed of RNA polymerase II. Proc Natl Acad Sci U S A (2012) 1.51

A universal pathway for kinesin stepping. Nat Struct Mol Biol (2011) 1.43

E. coli NusG inhibits backtracking and accelerates pause-free transcription by promoting forward translocation of RNA polymerase. J Mol Biol (2010) 1.36

Picocalorimetry of transcription by RNA polymerase. Biophys J (2005) 1.33

Direct observation of the binding state of the kinesin head to the microtubule. Nature (2009) 1.32

Folding and unfolding single RNA molecules under tension. Curr Opin Chem Biol (2008) 1.26

Pulling on the nascent RNA during transcription does not alter kinetics of elongation or ubiquitous pausing. Mol Cell (2006) 1.26

Statistical kinetics of macromolecular dynamics. Biophys J (2005) 1.24

Optical tweezers study life under tension. Nat Photonics (2011) 1.22

Single-molecule studies of RNA polymerase: one singular sensation, every little step it takes. Mol Cell (2011) 1.21

Measurement of the effective focal shift in an optical trap. Opt Lett (2005) 1.19

Resource Letter: LBOT-1: Laser-based optical tweezers. Am J Phys (2003) 1.17

Structure of the transcriptional network controlling white-opaque switching in Candida albicans. Mol Microbiol (2013) 1.14

Direct observation of cotranscriptional folding in an adenine riboswitch. Science (2012) 1.13

Reconstructing folding energy landscapes by single-molecule force spectroscopy. Annu Rev Biophys (2014) 1.12

The importance of lattice defects in katanin-mediated microtubule severing in vitro. Biophys J (2002) 1.07

Folding energy landscape of the thiamine pyrophosphate riboswitch aptamer. Proc Natl Acad Sci U S A (2012) 1.07

Identification and characterization of a previously undescribed family of sequence-specific DNA-binding domains. Proc Natl Acad Sci U S A (2013) 1.03

Precision steering of an optical trap by electro-optic deflection. Opt Lett (2008) 1.00

Applied force provides insight into transcriptional pausing and its modulation by transcription factor NusA. Mol Cell (2011) 0.97

Force and premature binding of ADP can regulate the processivity of individual Eg5 dimers. Biophys J (2009) 0.92

Direct measurements of kinesin torsional properties reveal flexible domains and occasional stalk reversals during stepping. Proc Natl Acad Sci U S A (2009) 0.91

Kinesin steps do not alternate in size. Biophys J (2007) 0.90

Transcription factors TFIIF and TFIIS promote transcript elongation by RNA polymerase II by synergistic and independent mechanisms. Proc Natl Acad Sci U S A (2014) 0.88

A not-so-cheap stunt. Science (2002) 0.88

On the origin of kinesin limping. Biophys J (2009) 0.88

Morphometric assessment of collagen accumulation in germinal matrix vessels of premature human neonates. Neuropathol Appl Neurobiol (2005) 0.87

Electrostatics of nucleic acid folding under conformational constraint. J Am Chem Soc (2012) 0.85

Efficient reconstitution of transcription elongation complexes for single-molecule studies of eukaryotic RNA polymerase II. Transcription (2012) 0.85

Anatomical analysis of the developing cerebral vasculature in premature neonates: absence of precapillary arteriole-to-venous shunts. Pediatr Res (2002) 0.82

Subependymal veins in premature neonates: implications for hemorrhage. Pediatr Neurol (2004) 0.81

Single-molecule studies of RNAPII elongation. Biochim Biophys Acta (2012) 0.80

An optical apparatus for rotation and trapping. Methods Enzymol (2010) 0.79

Not so lame after all: kinesin still walks with a hobbled head. J Gen Physiol (2007) 0.78

Microfluidic affinity and ChIP-seq analyses converge on a conserved FOXP2-binding motif in chimp and human, which enables the detection of evolutionarily novel targets. Nucleic Acids Res (2013) 0.78

Attitudes and expectations about music therapy for premature infants among staff in a neonatal intensive care unit. Altern Ther Health Med (2004) 0.78

Temporal expression pattern of cerebrovascular endothelial cell alkaline phosphatase during human gestation. J Neuropathol Exp Neurol (2002) 0.78

UK defence group's structure could limit its usefulness. Nature (2009) 0.75

Germinal matrix cells associate with veins and a glial scaffold in the human fetal brain. Brain Res Dev Brain Res (2005) 0.75