Transfection of mammalian cells with connexins and measurement of voltage sensitivity of their gap junctions.

PubWeight™: 1.04‹?› | Rank: Top 15%

🔗 View Article (PMID 17487162)

Published in Nat Protoc on January 01, 2006

Authors

Cristiane del Corsso1, Miduturu Srinivas, Marcia Urban-Maldonado, Alonso P Moreno, Alfredo G Fort, Glenn I Fishman, David C Spray

Author Affiliations

1: The Dominick P. Purpura Department of Neuroscience, Albert Einstein College of Medicine, 1410 Pelham Parkway South, Bronx, New York 10461, USA.

Articles citing this

Connexin and pannexin mediated cell-cell communication. Neuron Glia Biol (2007) 1.86

Gap junction remodeling and cardiac arrhythmogenesis in a murine model of oculodentodigital dysplasia. Proc Natl Acad Sci U S A (2007) 1.33

DNA-triggered innate immune responses are propagated by gap junction communication. Proc Natl Acad Sci U S A (2009) 1.17

Mefloquine blockade of Pannexin1 currents: resolution of a conflict. Cell Commun Adhes (2009) 1.12

Engineering biosynthetic excitable tissues from unexcitable cells for electrophysiological and cell therapy studies. Nat Commun (2011) 1.10

Functional cardiac tissue engineering. Regen Med (2012) 1.07

Characterizing functional stem cell-cardiomyocyte interactions. Regen Med (2010) 0.92

Calmodulin dependent protein kinase increases conductance at gap junctions formed by the neuronal gap junction protein connexin36. Brain Res (2012) 0.87

Matrix-dependent adhesion mediates network responses to physiological stimulation of the osteocyte cell process. Proc Natl Acad Sci U S A (2013) 0.87

Impact of Astroglial Connexins on Modafinil Pharmacological Properties. Sleep (2016) 0.83

Silencing MaxiK activity in corporal smooth muscle cells initiates compensatory mechanisms to maintain calcium homeostasis. J Sex Med (2011) 0.82

Pannexin1-mediated ATP release provides signal transmission between Neuro2A cells. Neurochem Res (2012) 0.79

Coupled Activation of Primary Sensory Neurons Contributes to Chronic Pain. Neuron (2016) 0.78

Heterotypic connexin50/connexin50 mutant gap junction channels reveal interactions between two hemichannels during transjunctional voltage-dependent gating. J Physiol (2012) 0.78

Quantifying electrical interactions between cardiomyocytes and other cells in micropatterned cell pairs. Methods Mol Biol (2014) 0.78

Cysteine residues in the cytoplasmic carboxyl terminus of connexins dictate gap junction plaque stability. Mol Biol Cell (2017) 0.75

Articles by these authors

Common variants in 22 loci are associated with QRS duration and cardiac ventricular conduction. Nat Genet (2010) 3.55

Reversion of gene expression alterations in hearts of mice with chronic chagasic cardiomyopathy after transplantation of bone marrow cells. Cell Cycle (2011) 3.49

Pannexin1 is part of the pore forming unit of the P2X(7) receptor death complex. FEBS Lett (2007) 3.19

Abnormal conduction and morphology in the atrioventricular node of mice with atrioventricular canal targeted deletion of Alk3/Bmpr1a receptor. Circulation (2007) 2.93

Conditional switching of VEGF provides new insights into adult neovascularization and pro-angiogenic therapy. EMBO J (2002) 2.74

Potent block of Cx36 and Cx50 gap junction channels by mefloquine. Proc Natl Acad Sci U S A (2004) 2.61

Regulation of peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1 alpha ) and mitochondrial function by MEF2 and HDAC5. Proc Natl Acad Sci U S A (2003) 2.48

Pannexin 1: the molecular substrate of astrocyte "hemichannels". J Neurosci (2009) 2.48

Modulation of cardiac gap junction expression and arrhythmic susceptibility. Circ Res (2004) 2.11

Subcellular heterogeneity of sodium current properties in adult cardiac ventricular myocytes. Heart Rhythm (2011) 2.10

The role of the glycocalyx in reorganization of the actin cytoskeleton under fluid shear stress: a "bumper-car" model. Proc Natl Acad Sci U S A (2004) 2.05

Isl1Cre reveals a common Bmp pathway in heart and limb development. Development (2006) 2.04

Structural changes in the carboxyl terminus of the gap junction protein connexin43 indicates signaling between binding domains for c-Src and zonula occludens-1. J Biol Chem (2004) 2.03

Imaging the endothelial glycocalyx in vitro by rapid freezing/freeze substitution transmission electron microscopy. Arterioscler Thromb Vasc Biol (2011) 1.89

Glial cells in (patho)physiology. J Neurochem (2012) 1.89

Connexin and pannexin mediated cell-cell communication. Neuron Glia Biol (2007) 1.86

Pannexin channels are not gap junction hemichannels. Channels (Austin) (2011) 1.84

Neuregulin-1 promotes formation of the murine cardiac conduction system. Proc Natl Acad Sci U S A (2002) 1.81

Connexin family members target to lipid raft domains and interact with caveolin-1. Biochemistry (2002) 1.66

Myocardial Notch signaling reprograms cardiomyocytes to a conduction-like phenotype. Circulation (2012) 1.66

Perspectives on Trypanosoma cruzi-induced heart disease (Chagas disease). Prog Cardiovasc Dis (2009) 1.60

pH-dependent intramolecular binding and structure involving Cx43 cytoplasmic domains. J Biol Chem (2002) 1.59

Connexin43 and connexin45 form heteromeric gap junction channels in which individual components determine permeability and regulation. Circ Res (2002) 1.54

Mechanisms of glutamate release from astrocytes: gap junction "hemichannels", purinergic receptors and exocytotic release. Neurochem Int (2004) 1.53

Gap junctions between cells expressing connexin 43 or 32 show inverse permselectivity to adenosine and ATP. J Biol Chem (2002) 1.49

Alk3/Bmpr1a receptor is required for development of the atrioventricular canal into valves and annulus fibrosus. Circ Res (2005) 1.48

The role of connexins in controlling cell growth and gene expression. Prog Biophys Mol Biol (2007) 1.48

Ephaptic conduction in a cardiac strand model with 3D electrodiffusion. Proc Natl Acad Sci U S A (2008) 1.46

Differentially altered Ca2+ regulation and Ca2+ permeability in Cx26 hemichannels formed by the A40V and G45E mutations that cause keratitis ichthyosis deafness syndrome. J Gen Physiol (2010) 1.45

Connexins, pannexins, innexins: novel roles of "hemi-channels". Pflugers Arch (2008) 1.45

Gap junctions mediate bystander cell death in developing retina. J Neurosci (2003) 1.43

Regulation of connexin43 protein complexes by intracellular acidification. Circ Res (2003) 1.43

Lamin A/C haploinsufficiency causes dilated cardiomyopathy and apoptosis-triggered cardiac conduction system disease. J Mol Cell Cardiol (2007) 1.42

Electrophysiological modeling of fibroblasts and their interaction with myocytes. Ann Biomed Eng (2007) 1.42

Gap junction remodeling and spironolactone-dependent reverse remodeling in the hypertrophied heart. Circ Res (2008) 1.41

Purkinje cells from RyR2 mutant mice are highly arrhythmogenic but responsive to targeted therapy. Circ Res (2010) 1.41

Blockade of gap junctions in vivo provides neuroprotection after perinatal global ischemia. Stroke (2005) 1.36

Loop gating of connexin hemichannels involves movement of pore-lining residues in the first extracellular loop domain. J Biol Chem (2008) 1.36

Contactin-2 expression in the cardiac Purkinje fiber network. Circ Arrhythm Electrophysiol (2010) 1.33

Gap junction remodeling and cardiac arrhythmogenesis in a murine model of oculodentodigital dysplasia. Proc Natl Acad Sci U S A (2007) 1.33

Phosphatase-resistant gap junctions inhibit pathological remodeling and prevent arrhythmias. Circ Res (2011) 1.32

Cell coupling between ventricular myocyte pairs from connexin43-deficient murine hearts. Circ Res (2003) 1.29

The neuronal connexin36 interacts with and is phosphorylated by CaMKII in a way similar to CaMKII interaction with glutamate receptors. Proc Natl Acad Sci U S A (2008) 1.28

New possible roles for aquaporin-4 in astrocytes: cell cytoskeleton and functional relationship with connexin43. FASEB J (2005) 1.28

pH-dependent dimerization of the carboxyl terminal domain of Cx43. Biophys J (2004) 1.26

Divalent cations regulate connexin hemichannels by modulating intrinsic voltage-dependent gating. J Gen Physiol (2008) 1.26

Array analysis of gene expression in connexin-43 null astrocytes. Physiol Genomics (2003) 1.24

Electrical remodeling contributes to complex tachyarrhythmias in connexin43-deficient mouse hearts. FASEB J (2007) 1.23

Proliferation of adult sertoli cells following conditional knockout of the Gap junctional protein GJA1 (connexin 43) in mice. Biol Reprod (2007) 1.23

Prospects for rational development of pharmacological gap junction channel blockers. Curr Drug Targets (2002) 1.22

Consequences of cardiac myocyte-specific ablation of KATP channels in transgenic mice expressing dominant negative Kir6 subunits. Am J Physiol Heart Circ Physiol (2006) 1.22

ATP signaling is deficient in cultured Pannexin1-null mouse astrocytes. Glia (2012) 1.22

Closure of gap junction channels by arylaminobenzoates. Mol Pharmacol (2003) 1.21

Acute downregulation of Cx43 alters P2Y receptor expression levels in mouse spinal cord astrocytes. Glia (2003) 1.21

Reduced intercellular coupling leads to paradoxical propagation across the Purkinje-ventricular junction and aberrant myocardial activation. Proc Natl Acad Sci U S A (2005) 1.21

Genes controlling multiple functional pathways are transcriptionally regulated in connexin43 null mouse heart. Physiol Genomics (2004) 1.20

Block of specific gap junction channel subtypes by 2-aminoethoxydiphenyl borate (2-APB). J Pharmacol Exp Ther (2006) 1.20

Microarray analysis of changes in gene expression in a murine model of chronic chagasic cardiomyopathy. Parasitol Res (2003) 1.19

Optimal lens epithelial cell proliferation is dependent on the connexin isoform providing gap junctional coupling. Invest Ophthalmol Vis Sci (2007) 1.19

Optimized labeling of bone marrow mesenchymal cells with superparamagnetic iron oxide nanoparticles and in vivo visualization by magnetic resonance imaging. J Nanobiotechnology (2011) 1.19

Fluid shear stress remodels expression and function of junctional proteins in cultured bone cells. Am J Physiol Cell Physiol (2002) 1.18

Cardiovascular dysregulation of miR-17-92 causes a lethal hypertrophic cardiomyopathy and arrhythmogenesis. FASEB J (2012) 1.17

Cognitive dysfunction in mice infected with Plasmodium berghei strain ANKA. J Infect Dis (2008) 1.16

The organization of adherens junctions and desmosomes at the cardiac intercalated disc is independent of gap junctions. J Cell Sci (2003) 1.16

Bone marrow cell therapy ameliorates and reverses chagasic cardiomyopathy in a mouse model. J Infect Dis (2008) 1.16

Conditional mineralocorticoid receptor expression in the heart leads to life-threatening arrhythmias. Circulation (2005) 1.15

Autophagy modulates dynamics of connexins at the plasma membrane in a ubiquitin-dependent manner. Mol Biol Cell (2012) 1.15

Sensitivity of the brain transcriptome to connexin ablation. Biochim Biophys Acta (2004) 1.14

Connexin43, the major gap junction protein of astrocytes, is down-regulated in inflamed white matter in an animal model of multiple sclerosis. J Neurosci Res (2005) 1.14

Subdiaphragmatic murine electrophysiological studies: sequential determination of ventricular refractoriness and arrhythmia induction. Am J Physiol Heart Circ Physiol (2003) 1.14

Mefloquine blockade of Pannexin1 currents: resolution of a conflict. Cell Commun Adhes (2009) 1.12

Gene expression alterations in connexin null mice extend beyond the gap junction. Neurochem Int (2004) 1.12

Functional characterization of a naturally occurring Cx50 truncation. Invest Ophthalmol Vis Sci (2006) 1.12

The cardiac conduction system. Circulation (2011) 1.11

Modifications in the biophysical properties of connexin43 channels by a peptide of the cytoplasmic loop region. Circ Res (2004) 1.11

Flow cytometry analysis of gap junction-mediated cell-cell communication: advantages and pitfalls. Cytometry A (2006) 1.10

Virtual cloning, functional expression, and gating analysis of human connexin31.9. Am J Physiol Cell Physiol (2002) 1.10

Acquired infection with Toxoplasma gondii in adult mice results in sensorimotor deficits but normal cognitive behavior despite widespread brain pathology. Microbes Infect (2010) 1.09

Cardiac connexins: genes to nexus. Adv Cardiol (2006) 1.08

Genetically engineered SCN5A mutant pig hearts exhibit conduction defects and arrhythmias. J Clin Invest (2014) 1.08

Focal gap junction uncoupling and spontaneous ventricular ectopy. Am J Physiol Heart Circ Physiol (2005) 1.08

Formation of the gap junction nexus: binding partners for connexins. J Physiol Paris (2002) 1.08

Similar transcriptomic alterations in Cx43 knockdown and knockout astrocytes. Cell Commun Adhes (2008) 1.07

Automated cell-based assay for screening of aquaporin inhibitors. Anal Chem (2009) 1.06

Connexin-43 in the osteogenic BM niche regulates its cellular composition and the bidirectional traffic of hematopoietic stem cells and progenitors. Blood (2012) 1.05

Sequence-specific resonance assignment of the carboxyl terminal domain of Connexin43. J Biomol NMR (2002) 1.04

Induction and patterning of the cardiac conduction system. Int J Dev Biol (2002) 1.03

Interaction between Connexin50 and mitogen-activated protein kinase signaling in lens homeostasis. Mol Biol Cell (2009) 1.03

Regulation of connexin43-protein binding in astrocytes in response to chemical ischemia/hypoxia. J Biol Chem (2004) 1.02