Dihydrofolate reductase: x-ray structure of the binary complex with methotrexate.

PubWeight™: 2.88‹?› | Rank: Top 1%

🔗 View Article (PMID 17920)

Published in Science on July 29, 1977

Authors

D A Matthews, R A Alden, J T Bolin, S T Freer, R Hamlin, N Xuong, J Kraut, M Poe, M Williams, K Hoogsteen

Articles citing this

Transformation with human dihydrofolate reductase renders malaria parasites insensitive to WR99210 but does not affect the intrinsic activity of proguanil. Proc Natl Acad Sci U S A (1997) 5.75

Molecular basis of differential resistance to cycloguanil and pyrimethamine in Plasmodium falciparum malaria. Proc Natl Acad Sci U S A (1990) 3.98

Crystallographic and molecular-orbital studies on the geometry of antifolate drugs. Biochem J (1980) 3.11

Construction of two Escherichia coli amber suppressor genes: tRNAPheCUA and tRNACysCUA. Proc Natl Acad Sci U S A (1986) 3.00

Evolutionary paths to antibiotic resistance under dynamically sustained drug selection. Nat Genet (2011) 2.86

Association of genetic mutations in Plasmodium vivax dhfr with resistance to sulfadoxine-pyrimethamine: geographical and clinical correlates. Antimicrob Agents Chemother (2001) 2.40

Novel point mutations in the dihydrofolate reductase gene of Plasmodium vivax: evidence for sequential selection by drug pressure. Antimicrob Agents Chemother (2003) 2.26

Identification of the type I trimethoprim-resistant dihydrofolate reductase specified by the Escherichia coli R-plasmid R483: comparison with procaryotic and eucaryotic dihydrofolate reductases. J Bacteriol (1983) 2.15

Dynamic dysfunction in dihydrofolate reductase results from antifolate drug binding: modulation of dynamics within a structural state. Structure (2009) 2.13

Elucidating the germination transcriptional program using small molecules. Plant Physiol (2008) 1.53

Limited polymorphism in the dihydropteroate synthetase gene (dhps) of Plasmodium vivax isolates from Thailand. Antimicrob Agents Chemother (2005) 1.33

Malarial dihydrofolate reductase as a paradigm for drug development against a resistance-compromised target. Proc Natl Acad Sci U S A (2012) 1.26

Crystal structure of dihydrofolate reductase from Plasmodium vivax: pyrimethamine displacement linked with mutation-induced resistance. Proc Natl Acad Sci U S A (2005) 1.22

Diagnostic chemical shift markers for loop conformation and substrate and cofactor binding in dihydrofolate reductase complexes. Protein Sci (2003) 1.20

Divergent evolution of protein conformational dynamics in dihydrofolate reductase. Nat Struct Mol Biol (2013) 1.11

Structure and expression of human dihydropteridine reductase. Proc Natl Acad Sci U S A (1987) 1.11

Evidence for dynamics in proteins as a mechanism for ligand dissociation. Nat Chem Biol (2012) 1.03

Molecular characterization of antifolates resistance-associated genes, (dhfr and dhps) in Plasmodium vivax isolates from the Middle East. Malar J (2009) 1.01

Long-range structural effects in a second-site revertant of a mutant dihydrofolate reductase. Proc Natl Acad Sci U S A (1993) 0.96

First three-dimensional structure of Toxoplasma gondii thymidylate synthase-dihydrofolate reductase: insights for catalysis, interdomain interactions, and substrate channeling. Biochemistry (2013) 0.95

Here be dragons: docking and screening in an uncharted region of chemical space. J Biomol Screen (2005) 0.94

Ultraviolet difference-spectroscopic studies of substrate and inhibitor binding to Lactobacillus casei dihydrofolate reductase. Biochem J (1978) 0.90

Expression of the plasmid-encoded type I dihydrofolate reductase gene in cultured mammalian cells: a novel selectable marker. Nucleic Acids Res (1988) 0.90

Histidine hydrogen-deuterium exchange mass spectrometry for probing the microenvironment of histidine residues in dihydrofolate reductase. PLoS One (2011) 0.89

Understanding resistance to combination chemotherapy. Drug Resist Updat (2012) 0.88

NMR structures of apo L. casei dihydrofolate reductase and its complexes with trimethoprim and NADPH: contributions to positive cooperative binding from ligand-induced refolding, conformational changes, and interligand hydrophobic interactions. Biochemistry (2011) 0.87

Crystal structure of the histone lysine specific demethylase LSD1 complexed with tetrahydrofolate. Protein Sci (2014) 0.86

Transition-state inhibitors of purine salvage and other prospective enzyme targets in malaria. Future Med Chem (2013) 0.83

Isolation and characterization of a variant dihydrofolate reductase cDNA from methotrexate-resistant murine L5178Y cells. Nucleic Acids Res (1990) 0.83

A 1H n.m.r. study of the role of the glutamate moiety in the binding of methotrexate to Lactobacillus casei dihydrofolate reductase. Br J Pharmacol (1984) 0.80

Differences in folate-protein interactions result in differing inhibition of native rat liver and recombinant glycine N-methyltransferase by 5-methyltetrahydrofolate. Biochim Biophys Acta (2011) 0.78

Validation of a Janus role of methotrexate-based PEGylated chitosan nanoparticles in vitro. Nanoscale Res Lett (2014) 0.76

Active site and remote contributions to catalysis in methylthioadenosine nucleosidases. Biochemistry (2015) 0.75

Circular-dichroism studies of ligand binding to dihydrofolate reductase from Lactobacillus casei MTX/R. Biochem J (1979) 0.75

Site specific polarization transfer from a hyperpolarized ligand of dihydrofolate reductase. J Biomol NMR (2016) 0.75

Quantitative PET Reporter Gene Imaging with [(11)C]Trimethoprim. Mol Ther (2017) 0.75

Ligand-directed dibromophenyl benzoate chemistry for rapid and selective acylation of intracellular natural proteins. Chem Sci (2015) 0.75

Articles by these authors

The sequence of the human genome. Science (2001) 101.55

Expression of VEGFR-2 and AC133 by circulating human CD34(+) cells identifies a population of functional endothelial precursors. Blood (2000) 9.24

Structure of large fragment of Escherichia coli DNA polymerase I complexed with dTMP. Nature (1985) 8.46

Intrinsic GTPase activity distinguishes normal and oncogenic ras p21 molecules. Proc Natl Acad Sci U S A (1984) 6.45

Mevinolin: a highly potent competitive inhibitor of hydroxymethylglutaryl-coenzyme A reductase and a cholesterol-lowering agent. Proc Natl Acad Sci U S A (1980) 6.31

Structures of ternary complexes of rat DNA polymerase beta, a DNA template-primer, and ddCTP. Science (1994) 5.57

A cytosolic binding protein for the immunosuppressant FK506 has peptidyl-prolyl isomerase activity but is distinct from cyclophilin. Nature (1989) 5.53

Loop and subdomain movements in the mechanism of Escherichia coli dihydrofolate reductase: crystallographic evidence. Biochemistry (1997) 5.46

Crystal structure of the ribonuclease H domain of HIV-1 reverse transcriptase. Science (1991) 5.42

Serine proteases: structure and mechanism of catalysis. Annu Rev Biochem (1977) 5.37

Nomenclature and classification of purinoceptors. Pharmacol Rev (1994) 5.32

Crystal structure of a complex between electron transfer partners, cytochrome c peroxidase and cytochrome c. Science (1992) 4.82

A Critical Study of the Methods of Estimating Cholesterol and Allied Substances. Biochem J (1921) 4.58

Crystal structures of human DNA polymerase beta complexed with gapped and nicked DNA: evidence for an induced fit mechanism. Biochemistry (1997) 4.55

A comparison of survival, growth, and pulmonary function in patients with cystic fibrosis in Boston and Toronto. J Clin Epidemiol (1988) 4.41

Regulation of elongation factor 2 kinase by p90(RSK1) and p70 S6 kinase. EMBO J (2001) 4.40

Lymphocyte trafficking and regional immunity. Adv Immunol (1999) 4.21

[3H]CGS 21680, a selective A2 adenosine receptor agonist directly labels A2 receptors in rat brain. J Pharmacol Exp Ther (1989) 4.18

Crystal structure of rat DNA polymerase beta: evidence for a common polymerase mechanism. Science (1994) 4.11

Distribution of NHS funds between fundholding and non-fundholding practices. BMJ (1994) 4.05

Structure of subtilisin BPN' at 2.5 angström resolution. Nature (1969) 3.98

Crystal structure of the catalytic subunit of cAMP-dependent protein kinase complexed with MgATP and peptide inhibitor. Biochemistry (1993) 3.88

Crystal structures of Escherichia coli and Lactobacillus casei dihydrofolate reductase refined at 1.7 A resolution. I. General features and binding of methotrexate. J Biol Chem (1982) 3.84

Enrichment of gene-coding sequences in maize by genome filtration. Science (2003) 3.41

Preserving renal function in adults with hypertension and diabetes: a consensus approach. National Kidney Foundation Hypertension and Diabetes Executive Committees Working Group. Am J Kidney Dis (2000) 3.37

Alternate-day prednisone reduces morbidity and improves pulmonary function in cystic fibrosis. Lancet (1985) 3.32

Deprivation and cause specific morbidity: evidence from the Somerset and Avon survey of health. BMJ (1996) 3.18

AHA Science Advisory. Resistance exercise in individuals with and without cardiovascular disease: benefits, rationale, safety, and prescription: An advisory from the Committee on Exercise, Rehabilitation, and Prevention, Council on Clinical Cardiology, American Heart Association; Position paper endorsed by the American College of Sports Medicine. Circulation (2000) 3.05

The 2.6-A crystal structure of Pseudomonas putida cytochrome P-450. J Biol Chem (1985) 2.95

Novel subunit-subunit interactions in the structure of glutamine synthetase. Nature (1986) 2.87

The mental health of patients with a chief complaint of chronic fatigue. A prospective evaluation and follow-up. Arch Intern Med (1988) 2.85

Reliability of the Washington University in St. Louis Kiddie Schedule for Affective Disorders and Schizophrenia (WASH-U-KSADS) mania and rapid cycling sections. J Am Acad Child Adolesc Psychiatry (2001) 2.83

Crystal structure of the biphenyl-cleaving extradiol dioxygenase from a PCB-degrading pseudomonad. Science (1995) 2.68

Final results from a meta-analysis of remedial interventions with drink/drive offenders. Addiction (1995) 2.66

Crystal structure of yeast cytochrome c peroxidase refined at 1.7-A resolution. J Biol Chem (1984) 2.56

Low-resolution electron-density and anomalous-scattering-density maps of Chromatium high-potential iron protein. J Mol Biol (1968) 2.46

Crystal structure of avian dihydrofolate reductase containing phenyltriazine and NADPH. J Biol Chem (1982) 2.45

Identification of inbred mouse strains harboring genetic modifiers of mammary tumor age of onset and metastatic progression. Int J Cancer (1998) 2.36

The stereochemistry of peroxidase catalysis. J Biol Chem (1980) 2.35

The three-dimensional structure of ricin at 2.8 A. J Biol Chem (1987) 2.34

Crystal structure of soybean lipoxygenase L-1 at 1.4 A resolution. Biochemistry (1996) 2.34

Depression and somatization in the chronic fatigue syndrome. Am J Med (1991) 2.33

Crystal structures of human DNA polymerase beta complexed with DNA: implications for catalytic mechanism, processivity, and fidelity. Biochemistry (1996) 2.25

Molecular recognition of the inhibitor AG-1343 by HIV-1 protease: conformationally flexible docking by evolutionary programming. Chem Biol (1995) 2.23

Chymotrypsinogen: 2.5-angstrom crystal structure, comparison with alpha-chymotrypsin, and implications for zymogen activation. Biochemistry (1970) 2.23

Double-blind and placebo-controlled study of lithium for adolescent bipolar disorders with secondary substance dependency. J Am Acad Child Adolesc Psychiatry (1998) 2.16

Incentive spirometry decreases respiratory complications following major abdominal surgery. Surgeon (2007) 2.15

Possible discrimination in recruitment of psychiatry residents? Am J Psychiatry (1997) 2.14

Subtilisin; a stereochemical mechanism involving transition-state stabilization. Biochemistry (1972) 2.13

The adenosine/neutrophil paradox resolved: human neutrophils possess both A1 and A2 receptors that promote chemotaxis and inhibit O2 generation, respectively. J Clin Invest (1990) 2.12

The frequency of the chronic fatigue syndrome in patients with symptoms of persistent fatigue. Ann Intern Med (1988) 2.11

Prepubertal and young adolescent bipolarity versus ADHD: assessment and validity using the WASH-U-KSADS, CBCL and TRF. J Affect Disord (1998) 2.10

Towards a revised nomenclature for P1 and P2 receptors. Trends Pharmacol Sci (1997) 2.09

Guidelines for the diagnosis and treatment of canine chronic valvular heart disease. J Vet Intern Med (2009) 2.06

Characterization of metabolites of the chemopreventive agent curcumin in human and rat hepatocytes and in the rat in vivo, and evaluation of their ability to inhibit phorbol ester-induced prostaglandin E2 production. Cancer Res (2001) 2.05

British HIV Association guidelines for the routine investigation and monitoring of adult HIV-1-infected individuals 2011. HIV Med (2012) 2.05

Multiwire area X-ray diffractometers. Methods Enzymol (1985) 2.04

Correlation between fragment size at D4F104S1 and age at onset or at wheelchair use, with a possible generational effect, accounts for much phenotypic variation in 4q35-facioscapulohumeral muscular dystrophy (FSHD) Hum Mol Genet (1995) 2.02

The segment polarity gene armadillo interacts with the wingless signaling pathway in both embryonic and adult pattern formation. Development (1991) 2.01

Adenosine receptors: pharmacology, structure-activity relationships, and therapeutic potential. J Med Chem (1992) 2.01

Quality of life measured by the St George's Respiratory Questionnaire and spirometry. Eur Respir J (2009) 1.99

CGS 21680C, an A2 selective adenosine receptor agonist with preferential hypotensive activity. J Pharmacol Exp Ther (1989) 1.99

Purine receptors in mammalian tissues: pharmacology and functional significance. Annu Rev Pharmacol Toxicol (1987) 1.97

Histopathology of the upper small intestines in typhoid fever. Biopsy study of experimental disease in man. Am J Dig Dis (1966) 1.97

Dihydrofolate reductase from Lactobacillus casei. X-ray structure of the enzyme methotrexate.NADPH complex. J Biol Chem (1978) 1.94

A comparison of Fe 4 S 4 clusters in high-potential iron protein and in ferredoxin. Proc Natl Acad Sci U S A (1972) 1.93

Crystallographic structure and functional interpretation of the cytoplasmic domain of erythrocyte membrane band 3. Blood (2000) 1.92

Detection of single mammalian cells by high-resolution magnetic resonance imaging. Biophys J (1999) 1.90

Directed mutagenesis of dihydrofolate reductase. Science (1983) 1.88

Evolutionary relationships among extradiol dioxygenases. J Bacteriol (1996) 1.88

Deciphering common failures in molecular docking of ligand-protein complexes. J Comput Aided Mol Des (2000) 1.87

The three-dimensional structure of Asn102 mutant of trypsin: role of Asp102 in serine protease catalysis. Science (1987) 1.87

Broad-spectrum, non-opioid analgesic activity by selective modulation of neuronal nicotinic acetylcholine receptors. Science (1998) 1.87

Crystal structure of unliganded Escherichia coli dihydrofolate reductase. Ligand-induced conformational changes and cooperativity in binding. Biochemistry (1991) 1.86

P2 purinergic receptors: modulation of cell function and therapeutic potential. J Pharmacol Exp Ther (2000) 1.85

Enzyme-DNA interactions required for efficient nucleotide incorporation and discrimination in human DNA polymerase beta. J Biol Chem (1996) 1.85

Synaptic plasma membranes from rat brain synaptosomes: isolation and partial characterization. Biochim Biophys Acta (1971) 1.84

Behavioural abnormalities and selective neuronal loss in HD transgenic mice expressing mutated full-length HD cDNA. Nat Genet (1998) 1.83

X-ray crystallographic study of boronic acid adducts with subtilisin BPN' (Novo). A model for the catalytic transition state. J Biol Chem (1975) 1.82

Identification of variable domains of the attachment (G) protein of subgroup A respiratory syncytial viruses. J Gen Virol (1991) 1.79

Human and murine cytotoxic T lymphocyte serine proteases: subsite mapping with peptide thioester substrates and inhibition of enzyme activity and cytolysis by isocoumarins. Biochemistry (1991) 1.77