Expression, purification and characterization of the secreted luciferase of the copepod Metridia longa from Sf9 insect cells.

PubWeight™: 0.81‹?›

🔗 View Article (PMC 2577724)

Published in Protein Expr Purif on June 10, 2008

Authors

Galina A Stepanyuk1, Hao Xu, Chia-Kuei Wu, Svetlana V Markova, John Lee, Eugene S Vysotski, Bi-Cheng Wang

Author Affiliations

1: Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, USA.

Articles cited by this

Oxidized redox state of glutathione in the endoplasmic reticulum. Science (1992) 6.91

Radical SAM, a novel protein superfamily linking unresolved steps in familiar biosynthetic pathways with radical mechanisms: functional characterization using new analysis and information visualization methods. Nucleic Acids Res (2001) 6.49

Codon-optimized Gaussia luciferase cDNA for mammalian gene expression in culture and in vivo. Mol Ther (2005) 6.04

Protein disulfide bond formation in prokaryotes. Annu Rev Biochem (2003) 3.76

A secreted luciferase for ex vivo monitoring of in vivo processes. Nat Methods (2008) 3.31

The crystal structure of the photoprotein aequorin at 2.3 A resolution. Nature (2000) 2.77

Red-shifted Renilla reniformis luciferase variants for imaging in living subjects. Nat Methods (2007) 2.64

In vitro folding of inclusion body proteins. FASEB J (1996) 2.40

Recombinant Gaussia luciferase. Overexpression, purification, and analytical application of a bioluminescent reporter for DNA hybridization. Anal Chem (2002) 1.91

Crystal structures of the luciferase and green fluorescent protein from Renilla reniformis. J Mol Biol (2007) 1.44

Cloning and expression of cDNA for a luciferase from the marine copepod Metridia longa. A novel secreted bioluminescent reporter enzyme. J Biol Chem (2003) 1.42

On the cysteine and cystine content of proteins. Differences between intracellular and extracellular proteins. J Mol Evol (1977) 1.30

Structure of the Ca2+-regulated photoprotein obelin at 1.7 A resolution determined directly from its sulfur substructure. Protein Sci (2000) 1.29

Ca2+-regulated photoproteins: structural insight into the bioluminescence mechanism. Acc Chem Res (2004) 1.25

The discovery of aequorin and green fluorescent protein. J Microsc (2005) 1.17

Real-time bioluminescence imaging of a protein secretory pathway in living mammalian cells using Gaussia luciferase. FEBS Lett (2007) 1.03

Identification of two catalytic domains in a luciferase secreted by the copepod Gaussia princeps. Biochem Biophys Res Commun (2007) 1.02

Interchange of aequorin and obelin bioluminescence color is determined by substitution of one active site residue of each photoprotein. FEBS Lett (2005) 1.00

Structural studies of the interaction of S-adenosylmethionine with the [4Fe-4S] clusters in biotin synthase and pyruvate formate-lyase activating enzyme. Protein Sci (2003) 0.94

Vargula hilgendorfii luciferase: a secreted reporter enzyme for monitoring gene expression in mammalian cells. Gene (1990) 0.90

Secretional luciferase of the luminous shrimp Oplophorus gracilirostris: cDNA cloning of a novel imidazopyrazinone luciferase(1). FEBS Lett (2000) 0.90

Synthesis of 13C-dehydrocoelenterazine and model studies on Symplectoteuthis squid bioluminescence. Bioorg Med Chem Lett (1998) 0.89

The oxidative folding of proteins by disulfide plus thiol does not correlate with redox potential. Protein Eng (1989) 0.89

Crystal structure of a Ca2+-discharged photoprotein: implications for mechanisms of the calcium trigger and bioluminescence. J Biol Chem (2004) 0.88

All three Ca2+-binding loops of photoproteins bind calcium ions: the crystal structures of calcium-loaded apo-aequorin and apo-obelin. Protein Sci (2005) 0.88

Structure of native Renilla reinformis luciferin. Proc Natl Acad Sci U S A (1977) 0.87

Imaging of luciferase secretion from transformed Chinese hamster ovary cells. Proc Natl Acad Sci U S A (1992) 0.86

Crystal structure of obelin after Ca2+-triggered bioluminescence suggests neutral coelenteramide as the primary excited state. Proc Natl Acad Sci U S A (2006) 0.86

Crystal structure of coelenterazine-binding protein from Renilla muelleri at 1.7 A: why it is not a calcium-regulated photoprotein. Photochem Photobiol Sci (2008) 0.86

Spectral tuning of obelin bioluminescence by mutations of Trp92. FEBS Lett (2003) 0.81

Continuous measurement of targeted promoter activity by a secreted bioluminescence reporter, Vargula hilgendorfii luciferase. Anal Biochem (2001) 0.79

Isolation and properties of the luciferase stored in the ovary of the scyphozoan medusa Periphylla periphylla. Biol Bull (2001) 0.77

Articles by these authors

The Genomes of Oryza sativa: a history of duplications. PLoS Biol (2005) 7.67

The NLRC4 inflammasome receptors for bacterial flagellin and type III secretion apparatus. Nature (2011) 6.21

A test of enhancing model accuracy in high-throughput crystallography. J Struct Funct Genomics (2005) 5.96

Disruption of Cnp1 uncouples oligodendroglial functions in axonal support and myelination. Nat Genet (2003) 5.43

Curved EFC/F-BAR-domain dimers are joined end to end into a filament for membrane invagination in endocytosis. Cell (2007) 4.73

The phosphothreonine lyase activity of a bacterial type III effector family. Science (2007) 3.18

Hedgehog/Wnt feedback supports regenerative proliferation of epithelial stem cells in bladder. Nature (2011) 3.15

Crystal structure of the cytoskeleton-associated protein glycine-rich (CAP-Gly) domain. J Biol Chem (2002) 3.01

Targeting the tumour vasculature: insights from physiological angiogenesis. Nat Rev Cancer (2010) 2.93

Granulocyte-colony stimulating factor promotes lung metastasis through mobilization of Ly6G+Ly6C+ granulocytes. Proc Natl Acad Sci U S A (2010) 2.77

JAK2V617F-mediated phosphorylation of PRMT5 downregulates its methyltransferase activity and promotes myeloproliferation. Cancer Cell (2011) 2.56

Reconstituted membrane fusion requires regulatory lipids, SNAREs and synergistic SNARE chaperones. EMBO J (2008) 2.16

Effects of D-4F on vasodilation and vessel wall thickness in hypercholesterolemic LDL receptor-null and LDL receptor/apolipoprotein A-I double-knockout mice on Western diet. Circ Res (2005) 2.06

Colonoscopy: practice variation among 69 hospital-based endoscopists. Gastrointest Endosc (2003) 2.02

Coronary bypass surgery performed off pump does not result in lower in-hospital morbidity than coronary artery bypass grafting performed on pump. Circulation (2004) 1.95

High levels of persistent expression of alpha1-antitrypsin mediated by the nonhuman primate serotype rh.10 adeno-associated virus despite preexisting immunity to common human adeno-associated viruses. Mol Ther (2005) 1.94

Foundations of representation: where might graphical symbol systems come from? Cogn Sci (2007) 1.80

Technical factors related to the incidence of adjacent superior segment facet joint violation after transpedicular instrumentation in the lumbar spine. Eur Spine J (2008) 1.75

Spontaneous resolution in patients with congenital Brown syndrome. J AAPOS (2008) 1.74

Induction of interleukin-12 and gamma interferon requires tumor necrosis factor alpha for protective T1-cell-mediated immunity to pulmonary Cryptococcus neoformans infection. Infect Immun (2002) 1.70

Study on the tongue manifestations for the blood-stasis and toxin syndrome in the stable patients of coronary heart disease. Chin J Integr Med (2011) 1.65

Impact of nodal metastases on prognosis in patients with well-differentiated thyroid cancer. Arch Otolaryngol Head Neck Surg (2002) 1.62

Photodynamic characterization and in vitro application of methylene blue-containing nanoparticle platforms. Photochem Photobiol (2005) 1.56

Diagnostic and clinical benefit of combined coronary calcium and perfusion assessment in patients undergoing PET/CT myocardial perfusion stress imaging. J Nucl Cardiol (2009) 1.55

Age-associated difference in circadian sleep-wake and rest-activity rhythms. Physiol Behav (2002) 1.53

Preoperative laparoscopic ligation of the left gastric vessels in preparation for esophagectomy. Ann Thorac Surg (2006) 1.52

Human thrombopoietin reduces myocardial infarct size, apoptosis, and stunning following ischaemia/reperfusion in rats. Cardiovasc Res (2007) 1.52

Laparoscopic transgastric access to the biliary tree after Roux-en-Y gastric bypass. Obes Surg (2007) 1.52

Cysteine methylation disrupts ubiquitin-chain sensing in NF-κB activation. Nature (2011) 1.50

The leukemogenicity of AML1-ETO is dependent on site-specific lysine acetylation. Science (2011) 1.49

A Legionella type IV effector activates the NF-kappaB pathway by phosphorylating the IkappaB family of inhibitors. Proc Natl Acad Sci U S A (2009) 1.48

Novel selective inhibitors of nuclear export CRM1 antagonists for therapy in mantle cell lymphoma. Exp Hematol (2012) 1.47

Endemic Venezuelan equine encephalitis in northern Peru. Emerg Infect Dis (2004) 1.47

The multifunctional human p100 protein 'hooks' methylated ligands. Nat Struct Mol Biol (2007) 1.46

Cloning and expression of cDNA for a luciferase from the marine copepod Metridia longa. A novel secreted bioluminescent reporter enzyme. J Biol Chem (2003) 1.42

Long-term functional results after ileal pouch anal restorative proctocolectomy for ulcerative colitis: a prospective observational study. Ann Surg (2003) 1.40

Nasal dorsal augmentation with freeze-dried allograft bone. Plast Reconstr Surg (2009) 1.40

XS0601 reduces the incidence of restenosis: a prospective study of 335 patients undergoing percutaneous coronary intervention in China. Chin Med J (Engl) (2006) 1.39

The Southeast Collaboratory for Structural Genomics: a high-throughput gene to structure factory. Acc Chem Res (2003) 1.39

Mechanism of class 1 (glycosylhydrolase family 47) {alpha}-mannosidases involved in N-glycan processing and endoplasmic reticulum quality control. J Biol Chem (2005) 1.38

Nanoparticles for two-photon photodynamic therapy in living cells. Nano Lett (2006) 1.37

Structure of D-AKAP2:PKA RI complex: insights into AKAP specificity and selectivity. Structure (2010) 1.36

2-Isopropyl-5-methyl-cyclo-hexyl N-cyclo-hexyl-P-phenyl-phospho-namidate. Acta Crystallogr Sect E Struct Rep Online (2010) 1.36

Life in the fast lane for protein crystallization and X-ray crystallography. Prog Biophys Mol Biol (2005) 1.33

Effects of D-4F on vasodilation, oxidative stress, angiostatin, myocardial inflammation, and angiogenic potential in tight-skin mice. Am J Physiol Heart Circ Physiol (2007) 1.32

An endoscopic approach to biliary complications following orthotopic liver transplantation. Liver Int (2003) 1.31

The role of BRAF mutation and p53 inactivation during transformation of a subpopulation of primary human melanocytes. Am J Pathol (2009) 1.31

Structure of dNTP-inducible dNTP triphosphohydrolase: insight into broad specificity for dNTPs and triphosphohydrolase-type hydrolysis. Acta Crystallogr D Biol Crystallogr (2007) 1.29

Identification of underexpressed genes in early- and late-stage primary ovarian tumors by suppression subtraction hybridization. Cancer Res (2002) 1.29

Comparison of individual and combination DNA vaccines for B. anthracis, Ebola virus, Marburg virus and Venezuelan equine encephalitis virus. Vaccine (2003) 1.29

Structural insights into the enzymatic mechanism of the pathogenic MAPK phosphothreonine lyase. Mol Cell (2007) 1.28

Ca2+-regulated photoproteins: structural insight into the bioluminescence mechanism. Acc Chem Res (2004) 1.25

Integrating traditional medicine with biomedicine towards a patient-centered healthcare system. Chin J Integr Med (2011) 1.25

A multi-center randomized double-blind placebo-controlled trial of Xiongshao Capsule in preventing restenosis after percutaneous coronary intervention: a subgroup analysis of senile patients. Chin J Integr Med (2011) 1.23

Development of biodegradable crosslinked urethane-doped polyester elastomers. Biomaterials (2008) 1.21

The crystal structure of augmenter of liver regeneration: A mammalian FAD-dependent sulfhydryl oxidase. Protein Sci (2003) 1.20

Parameter-space screening: a powerful tool for high-throughput crystal structure determination. Acta Crystallogr D Biol Crystallogr (2005) 1.20

Using telemedicine to conduct behavioral assessments. J Appl Behav Anal (2006) 1.20

Primary tracheobronchial amyloidosis in China: analysis of 64 cases and a review of literature. J Huazhong Univ Sci Technolog Med Sci (2010) 1.19

Sec18p and Vam7p remodel trans-SNARE complexes to permit a lipid-anchored R-SNARE to support yeast vacuole fusion. EMBO J (2007) 1.19

Quantum-dot-based immunofluorescent imaging of HER2 and ER provides new insights into breast cancer heterogeneity. Nanotechnology (2010) 1.18

Total synthesis of 1-O-methyllateriflorone. Angew Chem Int Ed Engl (2003) 1.17

TIP30/CC3 expression in breast carcinoma: relation to metastasis, clinicopathologic parameters, and P53 expression. Hum Pathol (2006) 1.16

Neuroimaging findings in macrocephaly-capillary malformation: a longitudinal study of 17 patients. Am J Med Genet A (2007) 1.16

In silico discovery of human natural antisense transcripts. BMC Bioinformatics (2006) 1.16

Randomized multicenter trial of foscarnet versus ganciclovir for preemptive therapy of cytomegalovirus infection after allogeneic stem cell transplantation. Blood (2002) 1.14

Size and shape determination of spheroidal scatterers using two-dimensional angle resolved scattering. Opt Express (2010) 1.12

Genetic deletion of apolipoprotein A-I increases airway hyperresponsiveness, inflammation, and collagen deposition in the lung. J Lipid Res (2010) 1.12

Adiponectin protects against cerebral ischemia-reperfusion injury through anti-inflammatory action. Brain Res (2009) 1.11

Genotypic and phenotypic features of citrin deficiency: five-year experience in a Chinese pediatric center. Int J Mol Med (2011) 1.10

Analysis on outcome of 5284 patients with coronary artery disease: the role of integrative medicine. J Ethnopharmacol (2011) 1.10

Stochastic optimal controller design for uncertain nonlinear networked control system via neuro dynamic programming. IEEE Trans Neural Netw Learn Syst (2013) 1.10

Forebrain NR2B overexpression facilitating the prefrontal cortex long-term potentiation and enhancing working memory function in mice. PLoS One (2011) 1.10

Effect of ERCP mechanical simulator (EMS) practice on trainees' ERCP performance in the early learning period: US multicenter randomized controlled trial. Am J Gastroenterol (2010) 1.10

Biomimetic total synthesis of gambogin and rate acceleration of pericyclic reactions in aqueous media. Angew Chem Int Ed Engl (2005) 1.10

Thirty-kilodalton Tat-interacting protein suppresses tumor metastasis by inhibition of osteopontin transcription in human hepatocellular carcinoma. Hepatology (2008) 1.09

Helicobacter pylori infection generated gastric cancer through p53-Rb tumor-suppressor system mutation and telomerase reactivation. World J Gastroenterol (2003) 1.09

Coracohumeral interval imaging in subcoracoid impingement syndrome on MRI. AJR Am J Roentgenol (2006) 1.08

The potential benefit of complementary/alternative medicine in cardiovascular diseases. Evid Based Complement Alternat Med (2012) 1.08

X-ray crystal structures of reduced rubrerythrin and its azide adduct: a structure-based mechanism for a non-heme diiron peroxidase. J Am Chem Soc (2002) 1.08

Clinicopathological features associated with lymph node metastasis in early gastric cancer: analysis of a single-institution experience in China. Can J Gastroenterol (2009) 1.08

Encapsulation of methylene blue in polyacrylamide nanoparticle platforms protects its photodynamic effectiveness. Biochem Biophys Res Commun (2008) 1.07

Biomimetically inspired total synthesis and structure activity relationships of 1-O-methyllateriflorone. 6 pi electrocyclizations in organic synthesis. J Am Chem Soc (2004) 1.07

Tanshinone IIA: A Promising Natural Cardioprotective Agent. Evid Based Complement Alternat Med (2012) 1.07

Characterization of soybean genomic features by analysis of its expressed sequence tags. Theor Appl Genet (2003) 1.06

Complementary and alternative medicine: is it possible to be mainstream? Chin J Integr Med (2012) 1.06

(NZ)CH...O contacts assist crystallization of a ParB-like nuclease. BMC Struct Biol (2007) 1.06

Structural basis for the exocellulase activity of the cellobiohydrolase CbhA from Clostridium thermocellum. Biochemistry (2004) 1.06

Structural evidence that brain cyclic nucleotide phosphodiesterase is a member of the 2H phosphodiesterase superfamily. J Biol Chem (2003) 1.04

TIP30 inhibits growth of HCC cell lines and inhibits HCC xenografts in mice in combination with 5-FU. Hepatology (2006) 1.03

The morphogenesis of wormian bones: a study of craniosynostosis and purposeful cranial deformation. Am J Med Genet A (2007) 1.03

miR-874 Inhibits cell proliferation, migration and invasion through targeting aquaporin-3 in gastric cancer. J Gastroenterol (2013) 1.03

Expression profile of multiple aquaporins in human gastric carcinoma and its clinical significance. Biomed Pharmacother (2010) 1.02

The ability of MLL to bind RUNX1 and methylate H3K4 at PU.1 regulatory regions is impaired by MDS/AML-associated RUNX1/AML1 mutations. Blood (2011) 1.02

Bosma arhinia microphthalmia syndrome. Am J Med Genet A (2006) 1.01

Conserved genes in a path from commensalism to pathogenicity: comparative phylogenetic profiles of Staphylococcus epidermidis RP62A and ATCC12228. BMC Genomics (2006) 1.01

High-coverage proteome analysis reveals the first insight of protein modification systems in the pathogenic spirochete Leptospira interrogans. Cell Res (2009) 1.01

Optochemical nanosensor PEBBLEs: photonic explorers for bioanalysis with biologically localized embedding. Curr Opin Chem Biol (2004) 1.01

Crystal structures of tyrosyl-tRNA synthetases from Archaea. J Mol Biol (2005) 1.00