Published in Anal Biochem on July 26, 2008
An overview of enzymatic reagents for the removal of affinity tags. Protein Expr Purif (2011) 1.46
Activity of the Human Rhinovirus 3C Protease Studied in Various Buffers, Additives and Detergents Solutions for Recombinant Protein Production. PLoS One (2016) 1.40
The New York Consortium on Membrane Protein Structure (NYCOMPS): a high-throughput platform for structural genomics of integral membrane proteins. J Struct Funct Genomics (2010) 1.07
The variable detergent sensitivity of proteases that are utilized for recombinant protein affinity tag removal. Protein Expr Purif (2011) 0.96
Solution-NMR characterization of outer-membrane protein A from E. coli in lipid bilayer nanodiscs and detergent micelles. Chembiochem (2014) 0.93
Expression and purification of the membrane enzyme selenoprotein K. Protein Expr Purif (2012) 0.81
Green fluorescent protein (GFP)-based overexpression screening and characterization of AgrC, a Receptor protein of quorum sensing in Staphylococcus aureus. Int J Mol Sci (2013) 0.75
The use of differential scanning fluorimetry to detect ligand interactions that promote protein stability. Nat Protoc (2007) 9.92
Chemical screening methods to identify ligands that promote protein stability, protein crystallization, and structure determination. Proc Natl Acad Sci U S A (2006) 4.45
Rapid screening for improved solubility of small human proteins produced as fusion proteins in Escherichia coli. Protein Sci (2002) 2.51
Improved solubility of TEV protease by directed evolution. J Biotechnol (2005) 2.35
Structural basis for synthesis of inflammatory mediators by human leukotriene C4 synthase. Nature (2007) 2.17
The use of systematic N- and C-terminal deletions to promote production and structural studies of recombinant proteins. Protein Expr Purif (2007) 2.10
Calcium and pH-dependent packing and release of the gel-forming MUC2 mucin. Proc Natl Acad Sci U S A (2012) 2.10
Bicarbonate and functional CFTR channel are required for proper mucin secretion and link cystic fibrosis with its mucus phenotype. J Exp Med (2012) 2.08
The cyclin-dependent kinase 8 module sterically blocks Mediator interactions with RNA polymerase II. Proc Natl Acad Sci U S A (2006) 1.91
ATP-induced conformational dynamics in the AAA+ motor unit of magnesium chelatase. Structure (2010) 1.63
Crystal structure of conserved domains 1 and 2 of the human DEAD-box helicase DDX3X in complex with the mononucleotide AMP. J Mol Biol (2007) 1.58
Structural basis for induced formation of the inflammatory mediator prostaglandin E2. Proc Natl Acad Sci U S A (2008) 1.58
Single-particle cryoelectron microscopy analysis reveals the HIV-1 spike as a tripod structure. Proc Natl Acad Sci U S A (2010) 1.54
Structural basis for detoxification and oxidative stress protection in membranes. J Mol Biol (2006) 1.49
Crystal structure of thy1, a thymidylate synthase complementing protein from Thermotoga maritima at 2.25 A resolution. Proteins (2002) 1.45
Human microsomal prostaglandin E synthase-1: purification, functional characterization, and projection structure determination. J Biol Chem (2003) 1.39
The DEXD/H-box RNA helicase DDX19 is regulated by an {alpha}-helical switch. J Biol Chem (2009) 1.39
Increased hydrophobicity at the N terminus/membrane interface impairs gating of the severe combined immunodeficiency-related ORAI1 mutant. J Biol Chem (2009) 1.39
An affibody in complex with a target protein: structure and coupled folding. Proc Natl Acad Sci U S A (2003) 1.35
His tag effect on solubility of human proteins produced in Escherichia coli: a comparison between four expression vectors. J Struct Funct Genomics (2004) 1.31
Enabling IMAC purification of low abundance recombinant proteins from E. coli lysates. Nat Methods (2009) 1.23
Comparative structural analysis of human DEAD-box RNA helicases. PLoS One (2010) 1.21
Stress sensor triggers conformational response of the integral membrane protein microsomal glutathione transferase 1. Biochemistry (2004) 1.13
Zinc binding catalytic domain of human tankyrase 1. J Mol Biol (2008) 1.09
High-resolution structure of a BRICHOS domain and its implications for anti-amyloid chaperone activity on lung surfactant protein C. Proc Natl Acad Sci U S A (2012) 1.06
Engineering membrane protein overproduction in Escherichia coli. Protein Sci (2008) 1.03
Identification of key residues determining species differences in inhibitor binding of microsomal prostaglandin E synthase-1. J Biol Chem (2010) 1.02
Crystal structure of an iron-containing 1,3-propanediol dehydrogenase (TM0920) from Thermotoga maritima at 1.3 A resolution. Proteins (2004) 1.00
Effect of N-terminal solubility enhancing fusion proteins on yield of purified target protein. J Struct Funct Genomics (2006) 1.00
A new cryo-EM single-particle ab initio reconstruction method visualizes secondary structure elements in an ATP-fueled AAA+ motor. J Mol Biol (2007) 0.99
Magnetite nanoparticles can be coupled to microbubbles to support multimodal imaging. Biomacromolecules (2012) 0.99
Structural basis of the iron storage function of frataxin from single-particle reconstruction of the iron-loaded oligomer. Biochemistry (2008) 0.98
Catalysis within the lipid bilayer-structure and mechanism of the MAPEG family of integral membrane proteins. Curr Opin Struct Biol (2008) 0.96
A general, robust method for the quality control of intact proteins using LC-ESI-MS. J Chromatogr B Analyt Technol Biomed Life Sci (2007) 0.95
Location of substrate binding sites within the integral membrane protein microsomal glutathione transferase-1. Biochemistry (2007) 0.94
Turning of the receptor-binding domains opens up the murine leukaemia virus Env for membrane fusion. EMBO J (2008) 0.93
Structural insights into the mechanisms of Mg2+ uptake, transport, and gating by CorA. Proc Natl Acad Sci U S A (2012) 0.93
tmRNA.SmpB complex mimics native aminoacyl-tRNAs in the A site of stalled ribosomes. J Struct Biol (2009) 0.92
Liposome and protein based stealth nanoparticles. Faraday Discuss (2013) 0.90
Projection structure at 8 A resolution of the melibiose permease, an Na-sugar co-transporter from Escherichia coli. EMBO J (2002) 0.90
The crystal structure of a human PP2A phosphatase activator reveals a novel fold and highly conserved cleft implicated in protein-protein interactions. J Biol Chem (2006) 0.90
Subsequent events to GTP binding by the plant PsbO protein: structural changes, GTP hydrolysis and dissociation from the photosystem II complex. Biochim Biophys Acta (2006) 0.89
Three-dimensional structure of the sugar symporter melibiose permease from cryo-electron microscopy. J Struct Biol (2005) 0.89
Biochemical discrimination between selenium and sulfur 1: a single residue provides selenium specificity to human selenocysteine lyase. PLoS One (2012) 0.89
Cryo-EM reveals promoter DNA binding and conformational flexibility of the general transcription factor TFIID. Structure (2009) 0.88
A simple strategy towards membrane protein purification and crystallization. Int J Biol Macromol (2006) 0.88
The major peptidyl-prolyl isomerase activity in thylakoid lumen of plant chloroplasts belongs to a novel cyclophilin TLP20. FEBS Lett (2003) 0.88
Co2+ selectivity of Thermotoga maritima CorA and its inability to regulate Mg2+ homeostasis present a new class of CorA proteins. J Biol Chem (2011) 0.87
Subunit arrangement in the dodecameric chloroplast small heat shock protein Hsp21. Protein Sci (2010) 0.87
Screening methods to determine biophysical properties of proteins in structural genomics. Anal Biochem (2003) 0.86
Exploring the structure and function of Thermotoga maritima CorA reveals the mechanism of gating and ion selectivity in Co2+/Mg2+ transport. Biochem J (2013) 0.86
On the interplay of shell structure with low- and high-frequency mechanics of multifunctional magnetic microbubbles. Soft Matter (2014) 0.86
A cost-effective method for simultaneous homo-oligomeric size determination and monodispersity conditions for membrane proteins. Anal Biochem (2011) 0.86
Crystal structure of uronate isomerase (TM0064) from Thermotoga maritima at 2.85 A resolution. Proteins (2003) 0.84
Transmembrane topology of FRO2, a ferric chelate reductase from Arabidopsis thaliana. Plant Mol Biol (2006) 0.84
Microsomal glutathione transferase 1 exhibits one-third-of-the-sites-reactivity towards glutathione. Arch Biochem Biophys (2009) 0.84
Crystal structure of the ATPase domain of the human AAA+ protein paraplegin/SPG7. PLoS One (2009) 0.82
The solution structure of ribosomal protein L18 from Thermus thermophilus reveals a conserved RNA-binding fold. Biochem J (2002) 0.82
Crystal structure of a novel Thermotoga maritima enzyme (TM1112) from the cupin family at 1.83 A resolution. Proteins (2004) 0.82
Structure of human argininosuccinate synthetase. Acta Crystallogr D Biol Crystallogr (2008) 0.81
Structural determination of functional domains in early B-cell factor (EBF) family of transcription factors reveals similarities to Rel DNA-binding proteins and a novel dimerization motif. J Biol Chem (2010) 0.81
1H, 13C and 15N resonance assignments of an affibody-target complex. J Biomol NMR (2002) 0.81
Strategies for the purification and on-column cleavage of glutathione-S-transferase fusion target proteins. J Chromatogr B Analyt Technol Biomed Life Sci (2002) 0.80
The 3-D structure of microsomal glutathione transferase 1 at 6 A resolution as determined by electron crystallography of p22(1)2(1) crystals. Biochim Biophys Acta (2002) 0.80
The projection structure of Kch, a putative potassium channel in Escherichia coli, by electron crystallography. Biochim Biophys Acta (2013) 0.79
Crystal structure of a zinc-containing glycerol dehydrogenase (TM0423) from Thermotoga maritima at 1.5 A resolution. Proteins (2003) 0.79
The mechanisms of Mg2+ and Co2+ transport by the CorA family of divalent cation transporters. Curr Top Membr (2012) 0.79
Rapana thomasiana hemocyanin (RtH): comparison of the two isoforms, RtH1 and RtH2, at 19A and 16A resolution. Micron (2006) 0.78
Crystal structure of O-acetylserine sulfhydrylase (TM0665) from Thermotoga maritima at 1.8 A resolution. Proteins (2004) 0.77
Single particle refinement in electron crystallography: a pilot study. J Struct Biol (2007) 0.76
Comprehensive analysis and identification of the human STIM1 domains for structural and functional studies. PLoS One (2013) 0.75
Assembly of Kch, a putative potassium channel from Escherichia coli. J Struct Biol (2009) 0.75
High-level expression, purification, and crystallization of recombinant rat leukotriene C(4) synthase from the yeast Pichia pastoris. Protein Expr Purif (2008) 0.75
Expression and purification of the recombinant membrane protein YidC: a case study for increased stability and solubility. Protein Expr Purif (2008) 0.75
A systematic approach to isolate mono-disperse membrane proteins - purification of zinc transporter ZntB. Protein Expr Purif (2010) 0.75