Proteomic profiling of Plasmodium sporozoite maturation identifies new proteins essential for parasite development and infectivity.

PubWeight™: 1.98‹?› | Rank: Top 2%

🔗 View Article (PMC 2570797)

Published in PLoS Pathog on October 31, 2008

Authors

Edwin Lasonder1, Chris J Janse, Geert-Jan van Gemert, Gunnar R Mair, Adriaan M W Vermunt, Bruno G Douradinha, Vera van Noort, Martijn A Huynen, Adrian J F Luty, Hans Kroeze, Shahid M Khan, Robert W Sauerwein, Andrew P Waters, Matthias Mann, Hendrik G Stunnenberg

Author Affiliations

1: Department of Molecular Biology, NCMLS, Radboud University Nijmegen, Nijmegen, The Netherlands.

Articles citing this

Visualisation and quantitative analysis of the rodent malaria liver stage by real time imaging. PLoS One (2009) 2.18

The Plasmodium eukaryotic initiation factor-2alpha kinase IK2 controls the latency of sporozoites in the mosquito salivary glands. J Exp Med (2010) 2.08

Retracted Branched tricarboxylic acid metabolism in Plasmodium falciparum. Nature (2010) 1.92

Protein export marks the early phase of gametocytogenesis of the human malaria parasite Plasmodium falciparum. Mol Cell Proteomics (2010) 1.92

Universal features of post-transcriptional gene regulation are critical for Plasmodium zygote development. PLoS Pathog (2010) 1.90

Three members of the 6-cys protein family of Plasmodium play a role in gamete fertility. PLoS Pathog (2010) 1.72

Preerythrocytic, live-attenuated Plasmodium falciparum vaccine candidates by design. Proc Natl Acad Sci U S A (2009) 1.72

Sterile protective immunity to malaria is associated with a panel of novel P. falciparum antigens. Mol Cell Proteomics (2011) 1.53

Malaria parasite development in the mosquito and infection of the mammalian host. Annu Rev Microbiol (2009) 1.52

Translocation of sickle cell erythrocyte microRNAs into Plasmodium falciparum inhibits parasite translation and contributes to malaria resistance. Cell Host Microbe (2012) 1.45

Harnessing genomics and genome biology to understand malaria biology. Nat Rev Genet (2012) 1.41

UIS2: A Unique Phosphatase Required for the Development of Plasmodium Liver Stages. PLoS Pathog (2016) 1.40

Reconstruction and flux-balance analysis of the Plasmodium falciparum metabolic network. Mol Syst Biol (2010) 1.37

Wolbachia infections in Anopheles gambiae cells: transcriptomic characterization of a novel host-symbiont interaction. PLoS Pathog (2011) 1.27

Life cycle studies of the hexose transporter of Plasmodium species and genetic validation of their essentiality. Mol Microbiol (2009) 1.25

Looking under the skin: the first steps in malarial infection and immunity. Nat Rev Microbiol (2013) 1.20

Total and putative surface proteomics of malaria parasite salivary gland sporozoites. Mol Cell Proteomics (2013) 1.19

Global analysis of protein expression and phosphorylation of three stages of Plasmodium falciparum intraerythrocytic development. J Proteome Res (2013) 1.15

In silico and biological survey of transcription-associated proteins implicated in the transcriptional machinery during the erythrocytic development of Plasmodium falciparum. BMC Genomics (2010) 1.12

Organellar proteomics reveals hundreds of novel nuclear proteins in the malaria parasite Plasmodium falciparum. Genome Biol (2012) 1.12

Plasmodium sporozoite-host interactions from the dermis to the hepatocyte. Curr Opin Microbiol (2009) 1.10

Plasmodium immunomics. Int J Parasitol (2010) 1.09

Pre-erythrocytic malaria vaccines: identifying the targets. Expert Rev Vaccines (2012) 1.07

The tumour antigen PRAME is a subunit of a Cul2 ubiquitin ligase and associates with active NFY promoters. EMBO J (2011) 1.03

Central carbon metabolism of Plasmodium parasites. Mol Biochem Parasitol (2010) 1.02

A rapid and scalable density gradient purification method for Plasmodium sporozoites. Malar J (2012) 1.01

Type II fatty acid biosynthesis is essential for Plasmodium falciparum sporozoite development in the midgut of Anopheles mosquitoes. Eukaryot Cell (2013) 0.98

Development of the piggyBac transposable system for Plasmodium berghei and its application for random mutagenesis in malaria parasites. BMC Genomics (2011) 0.97

Plasmodium falciparum proteome changes in response to doxycycline treatment. Malar J (2010) 0.96

Glutathione reductase-null malaria parasites have normal blood stage growth but arrest during development in the mosquito. J Biol Chem (2010) 0.96

Plasmodium falciparum PfA-M1 aminopeptidase is trafficked via the parasitophorous vacuole and marginally delivered to the food vacuole. Malar J (2010) 0.96

Perturbations of Plasmodium Puf2 expression and RNA-seq of Puf2-deficient sporozoites reveal a critical role in maintaining RNA homeostasis and parasite transmissibility. Cell Microbiol (2013) 0.95

Application of genomics to field investigations of malaria by the international centers of excellence for malaria research. Acta Trop (2011) 0.95

Predicting and exploring network components involved in pathogenesis in the malaria parasite via novel subnetwork alignments. BMC Syst Biol (2015) 0.94

Translational control in Plasmodium and toxoplasma parasites. Eukaryot Cell (2012) 0.93

Interrogating the Plasmodium Sporozoite Surface: Identification of Surface-Exposed Proteins and Demonstration of Glycosylation on CSP and TRAP by Mass Spectrometry-Based Proteomics. PLoS Pathog (2016) 0.91

Use of a selective inhibitor to define the chemotherapeutic potential of the plasmodial hexose transporter in different stages of the parasite's life cycle. Antimicrob Agents Chemother (2011) 0.91

A constitutive pan-hexose permease for the Plasmodium life cycle and transgenic models for screening of antimalarial sugar analogs. FASEB J (2010) 0.91

Recent Progress in Functional Genomic Research in Plasmodium falciparum. Curr Genomics (2010) 0.90

Identification of a major rif transcript common to gametocytes and sporozoites of Plasmodium falciparum. Malar J (2010) 0.88

Proteomic analysis of Plasmodium in the mosquito: progress and pitfalls. Parasitology (2012) 0.87

The evolutionary ecology of complex lifecycle parasites: linking phenomena with mechanisms. Heredity (Edinb) (2014) 0.87

A vacuolar iron-transporter homologue acts as a detoxifier in Plasmodium. Nat Commun (2016) 0.86

Plasmodial sugar transporters as anti-malarial drug targets and comparisons with other protozoa. Malar J (2011) 0.85

Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes: molecular insight into sex-specific processes and translational repression. Nucleic Acids Res (2016) 0.85

Module-based subnetwork alignments reveal novel transcriptional regulators in malaria parasite Plasmodium falciparum. BMC Syst Biol (2012) 0.84

Zinc finger nuclease-based double-strand breaks attenuate malaria parasites and reveal rare microhomology-mediated end joining. Genome Biol (2015) 0.84

Protease-associated cellular networks in malaria parasite Plasmodium falciparum. BMC Genomics (2011) 0.83

An efficient system to generate monoclonal antibodies against membrane-associated proteins by immunisation with antigen-expressing mammalian cells. BMC Biotechnol (2010) 0.83

RON12, a novel Plasmodium-specific rhoptry neck protein important for parasite proliferation. Cell Microbiol (2013) 0.82

Salivary gland-specific P. berghei reporter lines enable rapid evaluation of tissue-specific sporozoite loads in mosquitoes. PLoS One (2012) 0.82

The case for a rational genome-based vaccine against malaria. Front Microbiol (2015) 0.81

Topoisomerase II from Human Malaria Parasites: EXPRESSION, PURIFICATION, AND SELECTIVE INHIBITION. J Biol Chem (2015) 0.80

Indels, structural variation, and recombination drive genomic diversity in Plasmodium falciparum. Genome Res (2016) 0.80

Characterization of Plasmodium developmental transcriptomes in Anopheles gambiae midgut reveals novel regulators of malaria transmission. Cell Microbiol (2014) 0.80

Exploring systems affected by the heat shock response in Plasmodium falciparum via protein association networks. Int J Comput Biol Drug Des (2014) 0.79

Purine import into malaria parasites as a target for antimalarial drug development. Ann N Y Acad Sci (2014) 0.78

A small mitochondrial protein present in myzozoans is essential for malaria transmission. Open Biol (2016) 0.78

Reality check for malaria proteomics. Genome Biol (2009) 0.77

3D analysis of the TCR/pMHCII complex formation in monkeys vaccinated with the first peptide inducing sterilizing immunity against human malaria. PLoS One (2010) 0.77

Genome-Wide Collation of the Plasmodium falciparum WDR Protein Superfamily Reveals Malarial Parasite-Specific Features. PLoS One (2015) 0.77

A novel subnetwork alignment approach predicts new components of the cell cycle regulatory apparatus in Plasmodium falciparum. BMC Bioinformatics (2013) 0.77

Maternally supplied S-acyl-transferase is required for crystalloid organelle formation and transmission of the malaria parasite. Proc Natl Acad Sci U S A (2016) 0.77

Translational Control of UIS4 Protein of the Host-Parasite Interface Is Mediated by the RNA Binding Protein Puf2 in Plasmodium berghei Sporozoites. PLoS One (2016) 0.76

Dihydroquinazolinone inhibitors of proliferation of blood and liver stage malaria parasites. Antimicrob Agents Chemother (2013) 0.76

A Putative Small Solute Transporter Is Responsible for the Secretion of G377 and TRAP-Containing Secretory Vesicles during Plasmodium Gamete Egress and Sporozoite Motility. PLoS Pathog (2016) 0.76

Defining rules of CD8(+) T cell expansion against pre-erythrocytic Plasmodium antigens in sporozoite-immunized mice. Malar J (2016) 0.75

Translational regulation in blood stages of the malaria parasite Plasmodium spp.: systems-wide studies pave the way. Wiley Interdiscip Rev RNA (2016) 0.75

Epigenetic regulation of Plasmodium falciparum clonally variant gene expression during development in Anopheles gambiae. Sci Rep (2017) 0.75

Heavy path mining of protein-protein associations in the malaria parasite. Methods (2015) 0.75

Proteome mapping of Plasmodium: identification of the P. yoelii remodellome. Sci Rep (2016) 0.75

Comparative Genomics and Systems Biology of Malaria Parasites Plasmodium. Curr Bioinform (2012) 0.75

Malaria sporozoite proteome leaves a trail. Genome Biol (2009) 0.75

Comparative Plasmodium gene overexpression reveals distinct perturbation of sporozoite transmission by profilin. Mol Biol Cell (2016) 0.75

Plasmodium Cysteine Repeat Modular Proteins 3 and 4 are essential for malaria parasite transmission from the mosquito to the host. Malar J (2011) 0.75

Translational Control in the Latency of Apicomplexan Parasites. Trends Parasitol (2017) 0.75

Articles cited by this

BiNGO: a Cytoscape plugin to assess overrepresentation of gene ontology categories in biological networks. Bioinformatics (2005) 24.04

GO::TermFinder--open source software for accessing Gene Ontology information and finding significantly enriched Gene Ontology terms associated with a list of genes. Bioinformatics (2004) 20.23

The transcriptome of the intraerythrocytic developmental cycle of Plasmodium falciparum. PLoS Biol (2003) 11.86

Exponentially modified protein abundance index (emPAI) for estimation of absolute protein amount in proteomics by the number of sequenced peptides per protein. Mol Cell Proteomics (2005) 10.67

A proteomic view of the Plasmodium falciparum life cycle. Nature (2002) 10.65

Discovery of gene function by expression profiling of the malaria parasite life cycle. Science (2003) 10.57

PlasmoDB: the Plasmodium genome resource. A database integrating experimental and computational data. Nucleic Acids Res (2003) 6.19

A comprehensive survey of the Plasmodium life cycle by genomic, transcriptomic, and proteomic analyses. Science (2005) 5.99

A Plasmodium berghei reference line that constitutively expresses GFP at a high level throughout the complete life cycle. Mol Biochem Parasitol (2004) 5.80

Analysis of the Plasmodium falciparum proteome by high-accuracy mass spectrometry. Nature (2002) 5.48

Trypsin cleaves exclusively C-terminal to arginine and lysine residues. Mol Cell Proteomics (2004) 4.79

TRAP is necessary for gliding motility and infectivity of plasmodium sporozoites. Cell (1997) 4.65

Genetically modified Plasmodium parasites as a protective experimental malaria vaccine. Nature (2004) 4.35

Improved detection of overrepresentation of Gene-Ontology annotations with parent child analysis. Bioinformatics (2007) 4.32

High-efficiency transfection and drug selection of genetically transformed blood stages of the rodent malaria parasite Plasmodium berghei. Nat Protoc (2006) 4.19

Regulation of sexual development of Plasmodium by translational repression. Science (2006) 4.15

Migration of Plasmodium sporozoites through cells before infection. Science (2001) 3.89

Protein abundance profiling of the Escherichia coli cytosol. BMC Genomics (2008) 3.78

Proteome analysis of separated male and female gametocytes reveals novel sex-specific Plasmodium biology. Cell (2005) 3.77

Global analysis of transcript and protein levels across the Plasmodium falciparum life cycle. Genome Res (2004) 3.63

A combined transcriptome and proteome survey of malaria parasite liver stages. Proc Natl Acad Sci U S A (2008) 3.56

Intracellular parasite invasion strategies. Science (2004) 3.36

Plasmodium liver stage developmental arrest by depletion of a protein at the parasite-host interface. Proc Natl Acad Sci U S A (2005) 3.06

Plasmodium biology: genomic gleanings. Cell (2003) 2.89

A role for apical membrane antigen 1 during invasion of hepatocytes by Plasmodium falciparum sporozoites. J Biol Chem (2003) 2.84

A central role for P48/45 in malaria parasite male gamete fertility. Cell (2001) 2.73

Demonstration of heat-shock protein 70 in the sporozoite stage of malaria parasites. Parasitol Res (1994) 2.65

Cytoskeleton of apicomplexan parasites. Microbiol Mol Biol Rev (2002) 2.61

Genetically attenuated, P36p-deficient malarial sporozoites induce protective immunity and apoptosis of infected liver cells. Proc Natl Acad Sci U S A (2005) 2.49

Circumsporozoite protein is required for development of malaria sporozoites in mosquitoes. Nature (1997) 2.49

PlasmoDB: the Plasmodium genome resource. An integrated database providing tools for accessing, analyzing and mapping expression and sequence data (both finished and unfinished). Nucleic Acids Res (2002) 2.37

Migration through host cells activates Plasmodium sporozoites for infection. Nat Med (2002) 2.22

Infectivity of cultured Plasmodium falciparum gametocytes to mosquitoes. Parasitology (1989) 2.10

Characterization of Plasmodium falciparum sporozoite surface protein 2. Proc Natl Acad Sci U S A (1992) 2.00

Co-ordinated programme of gene expression during asexual intraerythrocytic development of the human malaria parasite Plasmodium falciparum revealed by microarray analysis. Mol Microbiol (2001) 1.96

Two Plasmodium rhomboid proteases preferentially cleave different adhesins implicated in all invasive stages of malaria. PLoS Pathog (2006) 1.95

Myosin A tail domain interacting protein (MTIP) localizes to the inner membrane complex of Plasmodium sporozoites. J Cell Sci (2003) 1.85

Shotgun DNA microarrays and stage-specific gene expression in Plasmodium falciparum malaria. Mol Microbiol (2000) 1.84

CelTOS, a novel malarial protein that mediates transmission to mosquito and vertebrate hosts. Mol Microbiol (2006) 1.84

Infectivity-associated changes in the transcriptional repertoire of the malaria parasite sporozoite stage. J Biol Chem (2002) 1.83

A Plasmodium sporozoite protein with a membrane attack complex domain is required for breaching the liver sinusoidal cell layer prior to hepatocyte infection. Cell Microbiol (2005) 1.83

Cell-passage activity is required for the malarial parasite to cross the liver sinusoidal cell layer. PLoS Biol (2004) 1.81

Serial analysis of gene expression in Plasmodium falciparum reveals the global expression profile of erythrocytic stages and the presence of anti-sense transcripts in the malarial parasite. Mol Biol Cell (2001) 1.79

Differential transcriptome profiling identifies Plasmodium genes encoding pre-erythrocytic stage-specific proteins. Mol Microbiol (2004) 1.76

Plasmodium sporozoite invasion into insect and mammalian cells is directed by the same dual binding system. EMBO J (2002) 1.73

A malarial cysteine protease is necessary for Plasmodium sporozoite egress from oocysts. J Exp Med (2005) 1.71

Two proteins with 6-cys motifs are required for malarial parasites to commit to infection of the hepatocyte. Mol Microbiol (2005) 1.64

Evidence-based annotation of the malaria parasite's genome using comparative expression profiling. PLoS One (2008) 1.61

Exploring the transcriptome of the malaria sporozoite stage. Proc Natl Acad Sci U S A (2001) 1.59

Selection by flow-sorting of genetically transformed, GFP-expressing blood stages of the rodent malaria parasite, Plasmodium berghei. Nat Protoc (2006) 1.50

von Willebrand Factor A domain-related protein, a novel microneme protein of the malaria ookinete highly conserved throughout Plasmodium parasites. Mol Biochem Parasitol (2001) 1.50

Apicomplexan gliding motility and host cell invasion: overhauling the motor model. Trends Parasitol (2004) 1.47

Pfs47, paralog of the male fertility factor Pfs48/45, is a female specific surface protein in Plasmodium falciparum. Mol Biochem Parasitol (2006) 1.42

MAEBL is essential for malarial sporozoite infection of the mosquito salivary gland. J Exp Med (2002) 1.41

A malaria membrane skeletal protein is essential for normal morphogenesis, motility, and infectivity of sporozoites. J Cell Biol (2004) 1.37

Manipulating the Plasmodium genome. Curr Issues Mol Biol (2005) 1.36

Ranking: a closer look on globalisation methods for normalisation of gene expression arrays. Nucleic Acids Res (2002) 1.35

A multidomain adhesion protein family expressed in Plasmodium falciparum is essential for transmission to the mosquito. J Exp Med (2004) 1.33

A malaria scavenger receptor-like protein essential for parasite development. Mol Microbiol (2002) 1.33

Selection of Anopheles stephensi for refractoriness and susceptibility to Plasmodium falciparum. Med Vet Entomol (1989) 1.28

Analysis of the Plasmodium and Anopheles transcriptomes during oocyst differentiation. J Biol Chem (2003) 1.26

Genetic disruption of the Plasmodium falciparum digestive vacuole plasmepsins demonstrates their functional redundancy. J Biol Chem (2004) 1.25

Evidence for a common role for the serine-type Plasmodium falciparum serine repeat antigen proteases: implications for vaccine and drug design. Infect Immun (2007) 1.23

A conserved U-rich RNA region implicated in regulation of translation in Plasmodium female gametocytes. Nucleic Acids Res (2007) 1.23

A surface phospholipase is involved in the migration of plasmodium sporozoites through cells. J Biol Chem (2004) 1.19

A long and winding road: the Plasmodium sporozoite's journey in the mammalian host. Parasitol Int (2007) 1.18

The Plasmodium TRAP/MIC2 family member, TRAP-Like Protein (TLP), is involved in tissue traversal by sporozoites. Cell Microbiol (2008) 1.17

Getting infectious: formation and maturation of Plasmodium sporozoites in the Anopheles vector. Cell Microbiol (2006) 1.14

Plasmodium falciparum sporozoite invasion is inhibited by naturally acquired or experimentally induced polyclonal antibodies to the STARP antigen. Eur J Immunol (1997) 1.10

PfSPATR, a Plasmodium falciparum protein containing an altered thrombospondin type I repeat domain is expressed at several stages of the parasite life cycle and is the target of inhibitory antibodies. J Biol Chem (2003) 1.09

Sporozoite load of mosquitoes infected with Plasmodium falciparum. Trans R Soc Trop Med Hyg (1990) 1.04

Exit of Plasmodium sporozoites from oocysts is an active process that involves the circumsporozoite protein. PLoS Pathog (2005) 1.03

Preerythrocytic malaria vaccine development. Curr Opin Infect Dis (2007) 1.03

The elusive malaria sporozoite in the mammalian host. Mol Microbiol (2004) 1.02

Plasmodium cysteine repeat modular proteins 1-4: complex proteins with roles throughout the malaria parasite life cycle. Cell Microbiol (2007) 1.01

Nomadic or sessile: can Kupffer cells function as portals for malaria sporozoites to the liver? Cell Microbiol (2006) 1.00

The Plasmodium falciparum knob-associated PfEMP3 antigen is also expressed at pre-erythrocytic stages and induces antibodies which inhibit sporozoite invasion. Mol Biochem Parasitol (2001) 0.99

Depletion of the Plasmodium berghei thrombospondin-related sporozoite protein reveals a role in host cell entry by sporozoites. Mol Biochem Parasitol (2007) 0.97

Vaccination against malaria with live parasites. Expert Rev Vaccines (2006) 0.96

Genetically modified Plasmodium highlights the potential of whole parasite vaccine strategies. Trends Immunol (2005) 0.94

Plasmodium falciparum phosphoenolpyruvate carboxykinase is developmentally regulated in gametocytes. Mol Biochem Parasitol (2000) 0.93

Liver invasion by malarial parasites--how do malarial parasites break through the host barrier? Cell Microbiol (2004) 0.92

Migration through host cells: the first steps of Plasmodium sporozoites in the mammalian host. Cell Microbiol (2004) 0.90

Serial Analysis of Gene Expression in Plasmodium berghei salivary gland sporozoites. BMC Genomics (2007) 0.89

New concepts in vaccine development in malaria. Curr Opin Infect Dis (2007) 0.86

Sporozoite and liver stage antigen Plasmodium falciparum peptides bind specifically to human hepatocytes. Vaccine (2004) 0.85

Integrative analysis of intraerythrocytic differentially expressed transcripts yields novel insights into the biology of Plasmodium falciparum. Malar J (2003) 0.80

Articles by these authors

MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification. Nat Biotechnol (2008) 38.00

Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry. Nature (2002) 37.66

Stable isotope labeling by amino acids in cell culture, SILAC, as a simple and accurate approach to expression proteomics. Mol Cell Proteomics (2002) 30.58

Mass spectrometry-based proteomics. Nature (2003) 28.94

Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Cell (2006) 20.92

Lysine acetylation targets protein complexes and co-regulates major cellular functions. Science (2009) 20.49

International network of cancer genome projects. Nature (2010) 20.35

miRNPs: a novel class of ribonucleoproteins containing numerous microRNAs. Genes Dev (2002) 19.24

Universal sample preparation method for proteome analysis. Nat Methods (2009) 16.88

In-gel digestion for mass spectrometric characterization of proteins and proteomes. Nat Protoc (2006) 16.56

Andromeda: a peptide search engine integrated into the MaxQuant environment. J Proteome Res (2011) 14.82

Stop and go extraction tips for matrix-assisted laser desorption/ionization, nanoelectrospray, and LC/MS sample pretreatment in proteomics. Anal Chem (2003) 13.58

An oestrogen-receptor-alpha-bound human chromatin interactome. Nature (2009) 12.16

Optimized design and assessment of whole genome tiling arrays. Bioinformatics (2007) 11.38

Protocol for micro-purification, enrichment, pre-fractionation and storage of peptides for proteomics using StageTips. Nat Protoc (2007) 10.71

Exponentially modified protein abundance index (emPAI) for estimation of absolute protein amount in proteomics by the number of sequenced peptides per protein. Mol Cell Proteomics (2005) 10.67

Parts per million mass accuracy on an Orbitrap mass spectrometer via lock mass injection into a C-trap. Mol Cell Proteomics (2005) 10.60

STRING: known and predicted protein-protein associations, integrated and transferred across organisms. Nucleic Acids Res (2005) 10.44

Comprehensive mass-spectrometry-based proteome quantification of haploid versus diploid yeast. Nature (2008) 10.28

The minimum information about a proteomics experiment (MIAPE). Nat Biotechnol (2007) 10.24

Genome sequence and comparative analysis of the model rodent malaria parasite Plasmodium yoelii yoelii. Nature (2002) 8.92

Proteomic characterization of the human centrosome by protein correlation profiling. Nature (2003) 8.74

Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal (2010) 8.61

Repeatability of published microarray gene expression analyses. Nat Genet (2008) 8.24

Mass spectrometry-based proteomics turns quantitative. Nat Chem Biol (2005) 7.52

Nucleolar proteome dynamics. Nature (2005) 7.32

Directed proteomic analysis of the human nucleolus. Curr Biol (2002) 7.04

Identification of a gene causing human cytochrome c oxidase deficiency by integrative genomics. Proc Natl Acad Sci U S A (2003) 6.87

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria. Cell (2003) 6.77

Selective anchoring of TFIID to nucleosomes by trimethylation of histone H3 lysine 4. Cell (2007) 6.62

Mechanism of regulation of WAVE1-induced actin nucleation by Rac1 and Nck. Nature (2002) 6.26

A comprehensive survey of the Plasmodium life cycle by genomic, transcriptomic, and proteomic analyses. Science (2005) 5.99

Quantitative comparison of genome-wide DNA methylation mapping technologies. Nat Biotechnol (2010) 5.96

The transcriptional and epigenomic foundations of ground state pluripotency. Cell (2012) 5.87

A Plasmodium berghei reference line that constitutively expresses GFP at a high level throughout the complete life cycle. Mol Biochem Parasitol (2004) 5.80

Large-scale proteomic analysis of the human spliceosome. Genome Res (2002) 5.75

Specificity in Toll-like receptor signalling through distinct effector functions of TRAF3 and TRAF6. Nature (2005) 5.72

Analysis of the Plasmodium falciparum proteome by high-accuracy mass spectrometry. Nature (2002) 5.48

Deep proteome and transcriptome mapping of a human cancer cell line. Mol Syst Biol (2011) 5.48

Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle. Mol Cell (2008) 5.38

ChIP-Seq of ERalpha and RNA polymerase II defines genes differentially responding to ligands. EMBO J (2009) 5.36

Complement-like protein TEP1 is a determinant of vectorial capacity in the malaria vector Anopheles gambiae. Cell (2004) 5.24

Temporal analysis of phosphotyrosine-dependent signaling networks by quantitative proteomics. Nat Biotechnol (2004) 5.22

A practical recipe for stable isotope labeling by amino acids in cell culture (SILAC). Nat Protoc (2006) 5.21

Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway. Mol Cell Proteomics (2005) 5.07

Higher-energy C-trap dissociation for peptide modification analysis. Nat Methods (2007) 5.04

SILAC mouse for quantitative proteomics uncovers kindlin-3 as an essential factor for red blood cell function. Cell (2008) 5.02

Genome-wide profiling of PPARgamma:RXR and RNA polymerase II occupancy reveals temporal activation of distinct metabolic pathways and changes in RXR dimer composition during adipogenesis. Genes Dev (2008) 4.93

Unbiased quantitative proteomics of lipid rafts reveals high specificity for signaling factors. Proc Natl Acad Sci U S A (2003) 4.87

Genomes in flux: the evolution of archaeal and proteobacterial gene content. Genome Res (2002) 4.85

A practical guide to the MaxQuant computational platform for SILAC-based quantitative proteomics. Nat Protoc (2009) 4.84

Trypsin cleaves exclusively C-terminal to arginine and lysine residues. Mol Cell Proteomics (2004) 4.79

Genome-wide analysis of ETS-family DNA-binding in vitro and in vivo. EMBO J (2010) 4.78

Proteome-wide analysis of chaperonin-dependent protein folding in Escherichia coli. Cell (2005) 4.78

A dual pressure linear ion trap Orbitrap instrument with very high sequencing speed. Mol Cell Proteomics (2009) 4.75

Hitting hotspots: spatial targeting of malaria for control and elimination. PLoS Med (2012) 4.68

PHOSIDA (phosphorylation site database): management, structural and evolutionary investigation, and prediction of phosphosites. Genome Biol (2007) 4.66

Transcriptome complexity in a genome-reduced bacterium. Science (2009) 4.64

A mammalian organelle map by protein correlation profiling. Cell (2006) 4.54

A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles. Mol Cell Proteomics (2011) 4.40

Quantitative interaction proteomics and genome-wide profiling of epigenetic histone marks and their readers. Cell (2010) 4.31

The ABC's (and XYZ's) of peptide sequencing. Nat Rev Mol Cell Biol (2004) 4.24

Human Proteinpedia enables sharing of human protein data. Nat Biotechnol (2008) 4.21

Submicroscopic Plasmodium falciparum gametocyte densities frequently result in mosquito infection. Am J Trop Med Hyg (2007) 4.20

Paraspeckles: a novel nuclear domain. Curr Biol (2002) 4.19

High-efficiency transfection and drug selection of genetically transformed blood stages of the rodent malaria parasite Plasmodium berghei. Nat Protoc (2006) 4.19

Regulation of sexual development of Plasmodium by translational repression. Science (2006) 4.15

Deciphering the evolution and metabolism of an anammox bacterium from a community genome. Nature (2006) 4.06

Protection against a malaria challenge by sporozoite inoculation. N Engl J Med (2009) 4.03

Quantitative proteomics reveals subset-specific viral recognition in dendritic cells. Immunity (2010) 4.03

Proteome organization in a genome-reduced bacterium. Science (2009) 3.97

High efficiency transfection of Plasmodium berghei facilitates novel selection procedures. Mol Biochem Parasitol (2005) 3.95

Axin-mediated CKI phosphorylation of beta-catenin at Ser 45: a molecular switch for the Wnt pathway. Genes Dev (2002) 3.80

The human urinary proteome contains more than 1500 proteins, including a large proportion of membrane proteins. Genome Biol (2006) 3.79

Protein abundance profiling of the Escherichia coli cytosol. BMC Genomics (2008) 3.78

Decoding signalling networks by mass spectrometry-based proteomics. Nat Rev Mol Cell Biol (2010) 3.78

Proteome analysis of separated male and female gametocytes reveals novel sex-specific Plasmodium biology. Cell (2005) 3.77

Mass spectrometry-based proteomics using Q Exactive, a high-performance benchtop quadrupole Orbitrap mass spectrometer. Mol Cell Proteomics (2011) 3.75

Bacille Calmette-Guerin induces NOD2-dependent nonspecific protection from reinfection via epigenetic reprogramming of monocytes. Proc Natl Acad Sci U S A (2012) 3.62

Quantitative proteomics combined with BAC TransgeneOmics reveals in vivo protein interactions. J Cell Biol (2010) 3.61

A TFTC/STAGA module mediates histone H2A and H2B deubiquitination, coactivates nuclear receptors, and counteracts heterochromatin silencing. Mol Cell (2008) 3.60

Precision mapping of an in vivo N-glycoproteome reveals rigid topological and sequence constraints. Cell (2010) 3.60