An improved in vitro and in vivo Sindbis virus expression system through host and virus engineering.

PubWeight™: 0.79‹?›

🔗 View Article (PMC 2730471)

Published in Virus Res on February 05, 2009

Authors

Toey Nivitchanyong1, Yien Che Tsai, Michael J Betenbaugh, George A Oyler

Author Affiliations

1: Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD, United States. Tarangsri.niv@biotec.or.th

Articles cited by this

Regulated translation initiation controls stress-induced gene expression in mammalian cells. Mol Cell (2000) 17.44

Protein translation and folding are coupled by an endoplasmic-reticulum-resident kinase. Nature (1999) 16.97

The alphaviruses: gene expression, replication, and evolution. Microbiol Rev (1994) 16.68

An integrated stress response regulates amino acid metabolism and resistance to oxidative stress. Mol Cell (2003) 15.12

CHOP is implicated in programmed cell death in response to impaired function of the endoplasmic reticulum. Genes Dev (1998) 11.86

Perk is essential for translational regulation and cell survival during the unfolded protein response. Mol Cell (2000) 11.09

ER stress triggers apoptosis by activating BH3-only protein Bim. Cell (2007) 7.50

A new generation of animal cell expression vectors based on the Semliki Forest virus replicon. Biotechnology (N Y) (1991) 7.08

Production of recombinant protein therapeutics in cultivated mammalian cells. Nat Biotechnol (2004) 5.67

Thapsigargin inhibits the sarcoplasmic or endoplasmic reticulum Ca-ATPase family of calcium pumps. J Biol Chem (1991) 4.92

Sindbis virus: an efficient, broad host range vector for gene expression in animal cells. Science (1989) 4.84

Sindbis virus expression vectors: packaging of RNA replicons by using defective helper RNAs. J Virol (1993) 4.19

Two distinct stress signaling pathways converge upon the CHOP promoter during the mammalian unfolded protein response. J Mol Biol (2002) 3.87

Parkin suppresses unfolded protein stress-induced cell death through its E3 ubiquitin-protein ligase activity. J Biol Chem (2000) 3.86

Bax-independent inhibition of apoptosis by Bcl-XL. Nature (1996) 3.82

Molecular mechanisms of interferon resistance mediated by viral-directed inhibition of PKR, the interferon-induced protein kinase. Pharmacol Ther (1998) 3.64

Activating transcription factor 3 is integral to the eukaryotic initiation factor 2 kinase stress response. Mol Cell Biol (2004) 3.57

Ligand-independent dimerization activates the stress response kinases IRE1 and PERK in the lumen of the endoplasmic reticulum. J Biol Chem (2000) 3.40

Regulation of apoptosis by endoplasmic reticulum pathways. Oncogene (2003) 3.32

Selection of RNA replicons capable of persistent noncytopathic replication in mammalian cells. J Virol (1999) 2.49

Noncytopathic Sindbis virus RNA vectors for heterologous gene expression. Proc Natl Acad Sci U S A (1998) 2.44

Japanese encephalitis virus infection initiates endoplasmic reticulum stress and an unfolded protein response. J Virol (2002) 2.41

Bc1-2 protects mice against fatal alphavirus encephalitis. Proc Natl Acad Sci U S A (1996) 2.31

Comparison of the effects of Sindbis virus and Sindbis virus replicons on host cell protein synthesis and cytopathogenicity in BHK cells. J Virol (1994) 2.25

Alphavirus-based expression vectors: strategies and applications. Proc Natl Acad Sci U S A (1996) 2.18

A model for the double-stranded RNA (dsRNA)-dependent dimerization and activation of the dsRNA-activated protein kinase PKR. J Biol Chem (1997) 2.13

Sindbis virus nonstructural protein nsP2 is cytotoxic and inhibits cellular transcription. J Virol (2006) 1.97

Viral determinants of age-dependent virulence of Sindbis virus for mice. J Virol (1993) 1.95

Translation of Sindbis virus mRNA: analysis of sequences downstream of the initiating AUG codon that enhance translation. J Virol (1996) 1.89

Neurovirulent strains of Alphavirus induce apoptosis in bcl-2-expressing cells: role of a single amino acid change in the E2 glycoprotein. Proc Natl Acad Sci U S A (1994) 1.84

Identification of a novel regulatory domain in Bcl-X(L) and Bcl-2. EMBO J (1997) 1.83

PKR-dependent and -independent mechanisms are involved in translational shutoff during Sindbis virus infection. J Virol (2004) 1.81

Identification of a novel function of the alphavirus capping apparatus. RNA 5'-triphosphatase activity of Nsp2. J Biol Chem (2000) 1.74

Sindbis virus induces apoptosis through a caspase-dependent, CrmA-sensitive pathway. J Virol (1998) 1.71

Establishment and maintenance of persistent infection by Sindbis virus in BHK cells. J Virol (1980) 1.66

Parkin facilitates the elimination of expanded polyglutamine proteins and leads to preservation of proteasome function. J Biol Chem (2003) 1.60

Identification of mutations in a Sindbis virus variant able to establish persistent infection in BHK cells: the importance of a mutation in the nsP2 gene. Virology (1997) 1.57

Alphavirus vectors for gene expression and vaccines. Curr Opin Biotechnol (1999) 1.34

Molecular analysis of neurovirulent strains of Sindbis virus that evolve during persistent infection of scid mice. J Virol (1993) 1.28

Inhibition of virus-induced neuronal apoptosis by Bax. Nat Med (1999) 1.22

The unfolded protein response. Mol Biotechnol (2006) 1.16

Inhibitor specificity of recombinant and endogenous caspase-9. Biochem J (2002) 1.07

Virus-induced apoptosis. Adv Pharmacol (1997) 1.07

A single mutation in the E2 glycoprotein important for neurovirulence influences binding of sindbis virus to neuroblastoma cells. J Virol (2002) 1.07

A single amino acid change in the E2 glycoprotein of Sindbis virus confers neurovirulence by altering an early step of virus replication. J Virol (1997) 1.03

Parkin and endoplasmic reticulum stress. Ann N Y Acad Sci (2003) 1.03

RNA viruses as gene expression vectors. Virus Genes (1989) 0.99

Biology and application of alphaviruses in gene therapy. Gene Ther (2005) 0.96

Aven and Bcl-xL enhance protection against apoptosis for mammalian cells exposed to various culture conditions. Biotechnol Bioeng (2004) 0.93

Combining caspase and mitochondrial dysfunction inhibitors of apoptosis to limit cell death in mammalian cell cultures. Biotechnol Bioeng (2006) 0.91

Enhanced cell culture performance using inducible anti-apoptotic genes E1B-19K and Aven in the production of a monoclonal antibody with Chinese hamster ovary cells. Biotechnol Bioeng (2007) 0.89

Inhibiting apoptosis in mammalian cell culture using the caspase inhibitor XIAP and deletion mutants. Biotechnol Bioeng (2002) 0.89

A comparison of the properties of a Bcl-xL variant to the wild-type anti-apoptosis inhibitor in mammalian cell cultures. Metab Eng (2003) 0.86

Comparison of Bcl-2 to a Bcl-2 deletion mutant for mammalian cells exposed to culture insults. Biotechnol Bioeng (2001) 0.86

Part I. Bcl-2 and Bcl-x(L) limit apoptosis upon infection with alphavirus vectors. Biotechnol Bioeng (2000) 0.85

Anti-apoptotic effect of Bcl-2 overexpression in RIN cells infected with Semliki Forest virus. Apoptosis (1997) 0.85

Study of caspase inhibitors for limiting death in mammalian cell culture. Biotechnol Bioeng (2003) 0.84

Alphaviruses as expression vectors. Curr Opin Biotechnol (1997) 0.82

Recent advances in gene expression using alphavirus vectors. Curr Opin Biotechnol (1998) 0.82

Alphavirus cDNA-based expression vectors: effects of RNA transcription and nuclear export. Biotechnol Bioeng (2003) 0.82

Antiapoptosis chemicals prolong productive lifetimes of mammalian cells upon Sindbis virus vector infection. Biotechnol Bioeng (1999) 0.81

Novel vectors expressing anti-apoptotic protein Bcl-2 to study cell death in Semliki Forest virus-infected cells. Virus Res (2007) 0.79

Articles by these authors

The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line. Nat Biotechnol (2011) 2.87

Structural basis for ubiquitin recognition and autoubiquitination by Rabex-5. Nat Struct Mol Biol (2006) 2.85

Inhibitors of ubiquitin-activating enzyme (E1), a new class of potential cancer therapeutics. Cancer Res (2007) 2.60

The activity of a human endoplasmic reticulum-associated degradation E3, gp78, requires its Cue domain, RING finger, and an E2-binding site. Proc Natl Acad Sci U S A (2006) 2.41

Ubiquitin ligases, critical mediators of endoplasmic reticulum-associated degradation. Semin Cell Dev Biol (2007) 2.13

Allosteric activation of E2-RING finger-mediated ubiquitylation by a structurally defined specific E2-binding region of gp78. Mol Cell (2009) 1.83

Differences in the tumor microenvironment between African-American and European-American breast cancer patients. PLoS One (2009) 1.72

A green light for engineered algae: redirecting metabolism to fuel a biotechnology revolution. Curr Opin Biotechnol (2008) 1.69

The Rab5 guanine nucleotide exchange factor Rabex-5 binds ubiquitin (Ub) and functions as a Ub ligase through an atypical Ub-interacting motif and a zinc finger domain. J Biol Chem (2006) 1.68

Parkin facilitates the elimination of expanded polyglutamine proteins and leads to preservation of proteasome function. J Biol Chem (2003) 1.60

Stress-induced phosphorylation and proteasomal degradation of mitofusin 2 facilitates mitochondrial fragmentation and apoptosis. Mol Cell (2012) 1.46

A mathematical model of N-linked glycosylation. Biotechnol Bioeng (2005) 1.42

Controlling N-linked glycan site occupancy. Biochim Biophys Acta (2005) 1.42

Enhanced transient recombinant protein production in CHO cells through the co-transfection of the product gene with Bcl-xL. Biotechnol J (2014) 1.38

Genomic landscapes of Chinese hamster ovary cell lines as revealed by the Cricetulus griseus draft genome. Nat Biotechnol (2013) 1.36

Comparing N-glycan processing in mammalian cell lines to native and engineered lepidopteran insect cell lines. Glycoconj J (2004) 1.27

Structure and synthesis of polyisoprenoids used in N-glycosylation across the three domains of life. Biochim Biophys Acta (2009) 1.17

Increased expression of the integral membrane protein ErbB2 in Chinese hamster ovary cells expressing the anti-apoptotic gene Bcl-xL. Protein Expr Purif (2009) 1.09

Chinese hamster genome database: an online resource for the CHO community at www.CHOgenome.org. Biotechnol Bioeng (2011) 1.08

Links between metabolism and apoptosis in mammalian cells: applications for anti-apoptosis engineering. Metab Eng (2007) 1.07

Accelerating genome editing in CHO cells using CRISPR Cas9 and CRISPy, a web-based target finding tool. Biotechnol Bioeng (2014) 1.04

Targeting of gp78 for ubiquitin-mediated proteasomal degradation by Hrd1: cross-talk between E3s in the endoplasmic reticulum. Biochem Biophys Res Commun (2009) 1.03

A mathematical model to derive N-glycan structures and cellular enzyme activities from mass spectrometric data. Glycobiology (2009) 1.01

BCL-xL-dependent light scattering by apoptotic cells. Biophys J (2004) 1.00

The emerging CHO systems biology era: harnessing the 'omics revolution for biotechnology. Curr Opin Biotechnol (2013) 0.98

Life and death in mammalian cell culture: strategies for apoptosis inhibition. Trends Biotechnol (2004) 0.97

The effect of mixotrophy on microalgal growth, lipid content, and expression levels of three pathway genes in Chlorella sorokiniana. Appl Microbiol Biotechnol (2011) 0.97

Engineering sialic acid synthetic ability into insect cells: identifying metabolic bottlenecks and devising strategies to overcome them. Biochemistry (2003) 0.96

Production and N-glycan analysis of secreted human erythropoietin glycoprotein in stably transfected Drosophila S2 cells. Biotechnol Bioeng (2005) 0.95

Expanding the spectral palette of fluorescent proteins for the green microalga Chlamydomonas reinhardtii. Plant J (2013) 0.95

Targeting botulinum neurotoxin persistence by the ubiquitin-proteasome system. Proc Natl Acad Sci U S A (2010) 0.94

False positive reactivity of recombinant, diagnostic, glycoproteins produced in High Five insect cells: effect of glycosylation. J Immunol Methods (2007) 0.93

Aven and Bcl-xL enhance protection against apoptosis for mammalian cells exposed to various culture conditions. Biotechnol Bioeng (2004) 0.93

An improved colony PCR procedure for genetic screening of Chlorella and related microalgae. Biotechnol Lett (2011) 0.91

Combining caspase and mitochondrial dysfunction inhibitors of apoptosis to limit cell death in mammalian cell cultures. Biotechnol Bioeng (2006) 0.91

Expression of a functional Drosophila melanogaster N-acetylneuraminic acid (Neu5Ac) phosphate synthase gene: evidence for endogenous sialic acid biosynthetic ability in insects. Glycobiology (2002) 0.91

Early prediction of instability of Chinese hamster ovary cell lines expressing recombinant antibodies and antibody-fusion proteins. Biotechnol Bioeng (2011) 0.90

Accelerated neuronal cell recovery from Botulinum neurotoxin intoxication by targeted ubiquitination. PLoS One (2011) 0.90

Humanization of lepidopteran insect-cell-produced glycoproteins. Acc Chem Res (2003) 0.90

Cellular trafficking and photochemical internalization of cell penetrating peptide linked cargo proteins: a dual fluorescent labeling study. Bioconjug Chem (2011) 0.89

Enhanced cell culture performance using inducible anti-apoptotic genes E1B-19K and Aven in the production of a monoclonal antibody with Chinese hamster ovary cells. Biotechnol Bioeng (2007) 0.89

Inhibiting apoptosis in mammalian cell culture using the caspase inhibitor XIAP and deletion mutants. Biotechnol Bioeng (2002) 0.89

Enhancement of transient gene expression and culture viability using Chinese hamster ovary cells overexpressing Bcl-x(L). Biotechnol Bioeng (2008) 0.89

GlycoFly: a database of Drosophila N-linked glycoproteins identified using SPEG--MS techniques. J Proteome Res (2011) 0.88

Engineering intracellular CMP-sialic acid metabolism into insect cells and methods to enhance its generation. Biochemistry (2005) 0.87

A comparison of the properties of a Bcl-xL variant to the wild-type anti-apoptosis inhibitor in mammalian cell cultures. Metab Eng (2003) 0.86

Purification, characterization, and cloning of a Spodoptera frugiperda Sf9 beta-N-acetylhexosaminidase that hydrolyzes terminal N-acetylglucosamine on the N-glycan core. J Biol Chem (2006) 0.85

Anterior thalamic mediation of experimental seizures: selective EEG spectral coherence. Epilepsia (2003) 0.85

Bcl-2 family in inter-organelle modulation of calcium signaling; roles in bioenergetics and cell survival. J Bioenerg Biomembr (2014) 0.85

Integration of the transcriptome and glycome for identification of glycan cell signatures. PLoS Comput Biol (2013) 0.85

Physiological evaluation of a new Chlorella sorokiniana isolate for its biomass production and lipid accumulation in photoautotrophic and heterotrophic cultures. Biotechnol Bioeng (2012) 0.84

Chemical caspase inhibitors enhance cell culture viabilities and protein titer. Biotechnol Prog (2007) 0.84

Study of caspase inhibitors for limiting death in mammalian cell culture. Biotechnol Bioeng (2003) 0.84

Expression of a functional Drosophila melanogaster CMP-sialic acid synthetase. Differential localization of the Drosophila and human enzymes. J Biol Chem (2006) 0.84

Optimization of tetracycline-responsive recombinant protein production and effect on cell growth and ER stress in mammalian cells. Biotechnol Bioeng (2005) 0.84

Expression of anti-apoptosis genes alters lactate metabolism of Chinese Hamster Ovary cells in culture. Biotechnol Bioeng (2009) 0.83

Mcl-1 overexpression leads to higher viabilities and increased production of humanized monoclonal antibody in Chinese hamster ovary cells. Biotechnol Prog (2009) 0.83

Complex-type biantennary N-glycans of recombinant human transferrin from Trichoplusia ni insect cells expressing mammalian [beta]-1,4-galactosyltransferase and [beta]-1,2-N-acetylglucosaminyltransferase II. Glycobiology (2002) 0.83

Directed evolution of mammalian anti-apoptosis proteins by somatic hypermutation. Protein Eng Des Sel (2011) 0.82

Generation of a phage-display library of single-domain camelid VH H antibodies directed against Chlamydomonas reinhardtii antigens, and characterization of VH Hs binding cell-surface antigens. Plant J (2013) 0.82

E2F-1 overexpression increases viable cell density in batch cultures of Chinese hamster ovary cells. J Biotechnol (2008) 0.81

Anti-apoptotic genes Aven and E1B-19K enhance performance of BHK cells engineered to express recombinant factor VIII in batch and low perfusion cell culture. Biotechnol Bioeng (2007) 0.81

Sinusoidal modeling of ictal activity along a thalamus-to-cortex seizure pathway I: new coherence approaches. Ann Biomed Eng (2004) 0.81

The C-terminal transmembrane domain of Bcl-xL mediates changes in mitochondrial morphology. Biophys J (2007) 0.81

Transient and stable expression of the neurotensin receptor NTS1: a comparison of the baculovirus-insect cell and the T-REx-293 expression systems. PLoS One (2013) 0.81

Proliferation and pluripotency of human embryonic stem cells maintained on type I collagen. Stem Cells Dev (2010) 0.80

Polyprenyl lipid synthesis in mammalian cells expressing human cis-prenyl transferase. Biochem Biophys Res Commun (2005) 0.80

Inhibition of neurotransmitter release by peptides that mimic the N-terminal domain of SNAP-25. J Protein Chem (2003) 0.79

Protein and genome evolution in Mammalian cells for biotechnology applications. Mol Biotechnol (2009) 0.79

Combining high-throughput screening of caspase activity with anti-apoptosis genes for development of robust CHO production cell lines. Biotechnol Prog (2010) 0.79

N-glycan structures of human transferrin produced by Lymantria dispar (gypsy moth) cells using the LdMNPV expression system. Glycobiology (2003) 0.79

Tyrosine phosphorylation of botulinum neurotoxin protease domains. Front Pharmacol (2012) 0.78

Bioprospecting of microalgae for integrated biomass production and phytoremediation of unsterilized wastewater and anaerobic digestion centrate. Appl Microbiol Biotechnol (2015) 0.78

Persistence of Botulinum neurotoxin inactivation of nerve function. Curr Top Microbiol Immunol (2013) 0.78

Significance of the parkin gene and protein in understanding Parkinson's disease. Curr Neurol Neurosci Rep (2002) 0.78

Feast or famine: autophagy control and engineering in eukaryotic cell culture. Curr Opin Biotechnol (2008) 0.78

Inhibiting the apoptosis pathway using MDM2 in mammalian cell cultures. Biotechnol Bioeng (2007) 0.78

Algal chloroplast produced camelid VH H antitoxins are capable of neutralizing botulinum neurotoxin. Plant Biotechnol J (2014) 0.78

Antigen retrieval to improve the immunocytochemistry detection of sigma-1 receptors and ER chaperones. Histochem Cell Biol (2011) 0.78

Rapid detection and quantification of triacylglycerol by HPLC-ELSD in Chlamydomonas reinhardtii and Chlorella strains. Lipids (2013) 0.78

Coupling enrichment methods with proteomics for understanding and treating disease. Proteomics Clin Appl (2015) 0.78

An HPLC-MALDI MS method for N-glycan analyses using smaller size samples: application to monitor glycan modulation by medium conditions. Glycoconj J (2009) 0.78

Retargeting Clostridium difficile Toxin B to Neuronal Cells as a Potential Vehicle for Cytosolic Delivery of Therapeutic Biomolecules to Treat Botulism. J Toxicol (2011) 0.78

Characterization of three Chlorella sorokiniana strains in anaerobic digested effluent from cattle manure. Bioresour Technol (2013) 0.77

The effect of iron on growth, lipid accumulation, and gene expression profile of the freshwater microalga Chlorella sorokiniana. Appl Microbiol Biotechnol (2014) 0.77

DmSAS is required for sialic acid biosynthesis in cultured Drosophila third instar larvae CNS neurons. ACS Chem Biol (2011) 0.77

Achieving high throughput sequencing of a cDNA library utilizing an alternative protocol for the bench top next-generation sequencing system. J Microbiol Methods (2012) 0.76

Tat-tetanus toxin fragment C: a novel protein delivery vector and its use with photochemical internalization. J Drug Target (2013) 0.76

Green algae as a platform to express therapeutic proteins. Discov Med (2009) 0.76