Epigenetic engineering of ribosomal RNA genes enhances protein production.

PubWeight™: 0.81‹?›

🔗 View Article (PMC 2721416)

Published in PLoS One on August 14, 2009

Authors

Raffaella Santoro1, Philipp Lienemann, Martin Fussenegger

Author Affiliations

1: Department of Biosystems Science and Engineering, ETH Zurich, Basel, Switzerland. raffaella.santoro@bsse.ethz.ch

Articles cited by this

Does the ribosome translate cancer? Nat Rev Cancer (2003) 6.89

Life on a planet of its own: regulation of RNA polymerase I transcription in the nucleolus. Genes Dev (2003) 3.85

The nucleolar remodeling complex NoRC mediates heterochromatin formation and silencing of ribosomal gene transcription. Nat Genet (2002) 3.58

Two different chromatin structures coexist in ribosomal RNA genes throughout the cell cycle. Cell (1989) 3.51

Nucleolar clustering of dispersed tRNA genes. Science (2003) 3.50

Intergenic transcripts regulate the epigenetic state of rRNA genes. Mol Cell (2006) 2.89

In exponentially growing Saccharomyces cerevisiae cells, rRNA synthesis is determined by the summed RNA polymerase I loading rate rather than by the number of active genes. Mol Cell Biol (2003) 2.88

RNA polymerases I and III, growth control and cancer. Nat Rev Mol Cell Biol (2005) 2.77

NoRC--a novel member of mammalian ISWI-containing chromatin remodeling machines. EMBO J (2001) 2.67

Engineered glycoforms of an antineuroblastoma IgG1 with optimized antibody-dependent cellular cytotoxic activity. Nat Biotechnol (1999) 2.44

The chromatin remodeling complex NoRC targets HDAC1 to the ribosomal gene promoter and represses RNA polymerase I transcription. EMBO J (2002) 2.36

Molecular mechanisms mediating methylation-dependent silencing of ribosomal gene transcription. Mol Cell (2001) 2.24

Role of human ribosomal RNA (rRNA) promoter methylation and of methyl-CpG-binding protein MBD2 in the suppression of rRNA gene expression. J Biol Chem (2003) 2.15

Controlled proliferation by multigene metabolic engineering enhances the productivity of Chinese hamster ovary cells. Nat Biotechnol (1998) 2.11

A housekeeper with power of attorney: the rRNA genes in ribosome biogenesis. Cell Mol Life Sci (2007) 1.98

Growth factor signaling regulates elongation of RNA polymerase I transcription in mammals via UBF phosphorylation and r-chromatin remodeling. Mol Cell (2006) 1.97

Coordinate regulation of ribosome biogenesis and function by the ribosomal protein S6 kinase, a key mediator of mTOR function. Growth Factors (2007) 1.89

Epigenetic mechanism of rRNA gene silencing: temporal order of NoRC-mediated histone modification, chromatin remodeling, and DNA methylation. Mol Cell Biol (2005) 1.66

Evaluating quantitative variation in the genome of Zea mays. Genetics (1986) 1.61

SAMY, a novel mammalian reporter gene derived from Bacillus stearothermophilus alpha-amylase. Gene (2002) 1.45

Epigenetic disruption of ribosomal RNA genes and nucleolar architecture in DNA methyltransferase 1 (Dnmt1) deficient cells. Nucleic Acids Res (2007) 1.42

The chromatin remodeling complex NoRC controls replication timing of rRNA genes. EMBO J (2004) 1.34

Nucleolar dominance: a model for rRNA gene silencing. Genes Dev (2006) 1.30

Frequent hypomethylation in Wilms tumors of pericentromeric DNA in chromosomes 1 and 16. Cancer Genet Cytogenet (1999) 1.25

The silence of the ribosomal RNA genes. Cell Mol Life Sci (2005) 1.14

Novel CNBP- and La-based translation control systems for mammalian cells. Biotechnol Bioeng (2003) 0.94

The ribosomal RNA gene cluster in aneuploid chickens: evidence for increased gene dosage and regulation of gene expression. J Cell Biol (1985) 0.86

Tight interaction between densely methylated DNA fragments and the methyl-CpG binding domain of the rat MeCP2 protein attached to a solid support. Biol Chem (1999) 0.82

Engineering mRNA translation initiation to enhance transient gene expression in chinese hamster ovary cells. Biotechnol Prog (2003) 0.81

Articles by these authors

A tunable synthetic mammalian oscillator. Nature (2009) 4.33

Method for generation of homogeneous multicellular tumor spheroids applicable to a wide variety of cell types. Biotechnol Bioeng (2003) 3.04

An engineered epigenetic transgene switch in mammalian cells. Nat Biotechnol (2004) 3.02

A synthetic optogenetic transcription device enhances blood-glucose homeostasis in mice. Science (2011) 2.77

Programmable single-cell mammalian biocomputers. Nature (2012) 2.56

Adult stem cell therapy of female stress urinary incontinence. Eur Urol (2007) 2.48

Retracted Autologous myoblasts and fibroblasts versus collagen for treatment of stress urinary incontinence in women: a randomised controlled trial. Lancet (2007) 2.35

Drug-sensing hydrogels for the inducible release of biopharmaceuticals. Nat Mater (2008) 2.23

Hysteresis in a synthetic mammalian gene network. Proc Natl Acad Sci U S A (2005) 2.12

Emerging biomedical applications of synthetic biology. Nat Rev Genet (2011) 2.05

BioLogic gates enable logical transcription control in mammalian cells. Biotechnol Bioeng (2004) 1.97

Synthetic two-way communication between mammalian cells. Nat Biotechnol (2012) 1.78

Synthetic ecosystems based on airborne inter- and intrakingdom communication. Proc Natl Acad Sci U S A (2007) 1.77

Macrolide-based transgene control in mammalian cells and mice. Nat Biotechnol (2002) 1.72

A closed-loop synthetic gene circuit for the treatment of diet-induced obesity in mice. Nat Commun (2013) 1.71

A synthetic mammalian gene circuit reveals antituberculosis compounds. Proc Natl Acad Sci U S A (2008) 1.68

Self-sufficient control of urate homeostasis in mice by a synthetic circuit. Nat Biotechnol (2010) 1.64

A synthetic time-delay circuit in mammalian cells and mice. Proc Natl Acad Sci U S A (2007) 1.59

Microscale tissue engineering using gravity-enforced cell assembly. Trends Biotechnol (2004) 1.55

Autologous myoblasts and fibroblasts for female stress incontinence: a 1-year follow-up in 123 patients. BJU Int (2007) 1.55

SAMY, a novel mammalian reporter gene derived from Bacillus stearothermophilus alpha-amylase. Gene (2002) 1.45

A synthetic erectile optogenetic stimulator enabling blue-light-inducible penile erection. Angew Chem Int Ed Engl (2015) 1.44

The NoRC complex mediates the heterochromatin formation and stability of silent rRNA genes and centromeric repeats. EMBO J (2010) 1.40

Advanced modular self-inactivating lentiviral expression vectors for multigene interventions in mammalian cells and in vivo transduction. Nucleic Acids Res (2002) 1.37

Design of custom-shaped vascularized tissues using microtissue spheroids as minimal building units. Tissue Eng (2006) 1.29

Gas-inducible transgene expression in mammalian cells and mice. Nat Biotechnol (2004) 1.27

Heat-stable oral alga-based vaccine protects mice from Staphylococcus aureus infection. J Biotechnol (2009) 1.26

An engineered mammalian band-pass network. Nucleic Acids Res (2010) 1.16

Mammalian synthetic biology: engineering of sophisticated gene networks. J Biotechnol (2007) 1.15

From gene switches to mammalian designer cells: present and future prospects. Trends Biotechnol (2012) 1.14

Design of artificial myocardial microtissues. Tissue Eng (2004) 1.12

Engineering of synthetic mammalian gene networks. Chem Biol (2009) 1.12

Streptomyces-derived quorum-sensing systems engineered for adjustable transgene expression in mammalian cells and mice. Nucleic Acids Res (2003) 1.07

Macrolide- and tetracycline-adjustable siRNA-mediated gene silencing in mammalian cells using polymerase II-dependent promoter derivatives. Biotechnol Bioeng (2004) 1.05

Artificial regulatory networks and cascades for discrete multilevel transgene control in mammalian cells. Biotechnol Bioeng (2003) 1.04

Controlling transgene expression in subcutaneous implants using a skin lotion containing the apple metabolite phloretin. Proc Natl Acad Sci U S A (2009) 1.03

Xbp1-based engineering of secretory capacity enhances the productivity of Chinese hamster ovary cells. Metab Eng (2006) 1.02

Artificial mammalian gene regulation networks-novel approaches for gene therapy and bioengineering. J Biotechnol (2002) 1.01

VEGF profiling and angiogenesis in human microtissues. J Biotechnol (2005) 1.00

A genetic time-delay circuitry in mammalian cells. Biotechnol Bioeng (2007) 1.00

Scaffold-free cell delivery for use in regenerative medicine. Adv Drug Deliv Rev (2010) 1.00

The food additive vanillic acid controls transgene expression in mammalian cells and mice. Nucleic Acids Res (2011) 0.99

Pharmacologic transgene control systems for gene therapy. J Gene Med (2006) 0.99

Molecular diversity--the toolbox for synthetic gene switches and networks. Curr Opin Chem Biol (2011) 0.99

Effects of protein and gene transfer of the angiopoietin-1 fibrinogen-like receptor-binding domain on endothelial and vessel organization. J Biol Chem (2005) 0.98

A designer network coordinating bovine artificial insemination by ovulation-triggered release of implanted sperms. J Control Release (2010) 0.97

A synthetic low-frequency mammalian oscillator. Nucleic Acids Res (2010) 0.97

A genetic redox sensor for mammalian cells. Metab Eng (2006) 0.97

An overview of the diverse roles of G-protein coupled receptors (GPCRs) in the pathophysiology of various human diseases. Biotechnol Adv (2013) 0.96

Vitamin H-regulated transgene expression in mammalian cells. Nucleic Acids Res (2007) 0.96

The use of light for engineered control and reprogramming of cellular functions. Curr Opin Biotechnol (2011) 0.96

Myoblast and fibroblast therapy for post-prostatectomy urinary incontinence: 1-year followup of 63 patients. J Urol (2007) 0.96

Synthetic mammalian gene circuits for biomedical applications. Curr Opin Chem Biol (2013) 0.95

Tissue-transplant fusion and vascularization of myocardial microtissues and macrotissues implanted into chicken embryos and rats. Tissue Eng (2006) 0.95

Intronically encoded siRNAs improve dynamic range of mammalian gene regulation systems and toggle switch. Nucleic Acids Res (2008) 0.95

Novel CNBP- and La-based translation control systems for mammalian cells. Biotechnol Bioeng (2003) 0.94

Modeling the quorum sensing regulatory network of human-pathogenic Pseudomonas aeruginosa. Biotechnol Prog (2004) 0.94

Biomedically relevant circuit-design strategies in mammalian synthetic biology. Mol Syst Biol (2013) 0.93

Recent advances in mammalian synthetic biology-design of synthetic transgene control networks. Curr Opin Biotechnol (2009) 0.93

Pharmaceutically controlled designer circuit for the treatment of the metabolic syndrome. Proc Natl Acad Sci U S A (2012) 0.93

A synthetic mammalian electro-genetic transcription circuit. Nucleic Acids Res (2009) 0.93

Toward construction of a self-sustained clock-like expression system based on the mammalian circadian clock. Biotechnol Bioeng (2004) 0.93

Multi-gene engineering: simultaneous expression and knockdown of six genes off a single platform. Biotechnol Bioeng (2007) 0.93

Therapeutic protein transduction of mammalian cells and mice by nucleic acid-free lentiviral nanoparticles. Nucleic Acids Res (2006) 0.92

Inducible product gene expression technology tailored to bioprocess engineering. Curr Opin Biotechnol (2007) 0.92

Conditional human VEGF-mediated vascularization in chicken embryos using a novel temperature-inducible gene regulation (TIGR) system. Nucleic Acids Res (2003) 0.92

Versatile macrolide-responsive mammalian expression vectors for multiregulated multigene metabolic engineering. Biotechnol Bioeng (2002) 0.91

Engineering of synthetic intercellular communication systems. Metab Eng (2012) 0.91

Impact of RNA interference on gene networks. Metab Eng (2006) 0.90

Dual-regulated expression of C/EBP-alpha and BMP-2 enables differential differentiation of C2C12 cells into adipocytes and osteoblasts. Nucleic Acids Res (2004) 0.90

Impact of coexpression and coamplification of sICAM and antiapoptosis determinants bcl-2/bcl-x(L) on productivity, cell survival, and mitochondria number in CHO-DG44 grown in suspension and serum-free media. Biotechnol Bioeng (2002) 0.90

An engineered L-arginine sensor of Chlamydia pneumoniae enables arginine-adjustable transcription control in mammalian cells and mice. Nucleic Acids Res (2007) 0.90

Ectopic expression of human mTOR increases viability, robustness, cell size, proliferation, and antibody production of chinese hamster ovary cells. Biotechnol Bioeng (2010) 0.89

Novel promoter/transactivator configurations for macrolide- and streptogramin-responsive transgene expression in mammalian cells. J Gene Med (2002) 0.89

Broad-spectrum protein biosensors for class-specific detection of antibiotics. Biotechnol Bioeng (2005) 0.89

Engineering molecular circuits using synthetic biology in mammalian cells. Annu Rev Chem Biomol Eng (2012) 0.89

Synthetic biology: Synchronized bacterial clocks. Nature (2010) 0.89

Smart medication through combination of synthetic biology and cell microencapsulation. Metab Eng (2011) 0.89

Semi-synthetic mammalian gene regulatory networks. Metab Eng (2005) 0.89

Transgene control engineering in mammalian cells. Methods Mol Biol (2005) 0.89

Ligand-dependent regulatory RNA parts for Synthetic Biology in eukaryotes. Curr Opin Biotechnol (2010) 0.89

Differential effect of exocytic SNAREs on the production of recombinant proteins in mammalian cells. Biotechnol Bioeng (2010) 0.88

A biotin-triggered genetic switch in mammalian cells and mice. Metab Eng (2009) 0.88

A novel reporter system for bacterial and mammalian cells based on the non-ribosomal peptide indigoidine. Metab Eng (2012) 0.87

Engineering of ribozyme-based riboswitches for mammalian cells. Methods (2012) 0.87

Modulation of cardiomyocyte electrical properties using regulated bone morphogenetic protein-2 expression. Tissue Eng Part A (2008) 0.87

The impact of synthetic biology on drug discovery. Drug Discov Today (2009) 0.87

Mammalian synthetic biology--from tools to therapies. Bioessays (2010) 0.86

Rational design of a small molecule-responsive intramer controlling transgene expression in mammalian cells. Nucleic Acids Res (2011) 0.86

Detailed design and comparative analysis of protocols for optimized production of high-performance HIV-1-derived lentiviral particles. Metab Eng (2005) 0.86

A novel mammalian expression system derived from components coordinating nicotine degradation in arthrobacter nicotinovorans pAO1. Nucleic Acids Res (2005) 0.86

Life after the synthetic cell. Nature (2010) 0.86

Design and in vivo characterization of self-inactivating human and non-human lentiviral expression vectors engineered for streptogramin-adjustable transgene expression. Nucleic Acids Res (2004) 0.86

De novo design and construction of an inducible gene expression system in mammalian cells. Methods Enzymol (2011) 0.86

Synthesis and characterization of PEG-based drug-responsive biohybrid hydrogels. Macromol Rapid Commun (2012) 0.85

Self-assembly of sensory neurons into ganglia-like microtissues. J Biotechnol (2005) 0.85

The vesicle-trafficking protein munc18b increases the secretory capacity of mammalian cells. Metab Eng (2009) 0.85

A novel system for trigger-controlled drug release from polymer capsules. J Control Release (2008) 0.85

Molecular engineering of exocytic vesicle traffic enhances the productivity of Chinese hamster ovary cells. Biotechnol Bioeng (2009) 0.84