Published in J Am Chem Soc on November 04, 2009
Proton-coupled electron transfer. Chem Rev (2007) 3.01
Reversible, long-range radical transfer in E. coli class Ia ribonucleotide reductase. Acc Chem Res (2013) 1.31
Equilibration of tyrosyl radicals (Y356•, Y731•, Y730•) in the radical propagation pathway of the Escherichia coli class Ia ribonucleotide reductase. J Am Chem Soc (2011) 1.21
Site-specific incorporation of 3-nitrotyrosine as a probe of pKa perturbation of redox-active tyrosines in ribonucleotide reductase. J Am Chem Soc (2010) 1.16
A hot oxidant, 3-NO2Y122 radical, unmasks conformational gating in ribonucleotide reductase. J Am Chem Soc (2010) 1.12
Kinetics of radical intermediate formation and deoxynucleotide production in 3-aminotyrosine-substituted Escherichia coli ribonucleotide reductases. J Am Chem Soc (2011) 1.09
Deciphering radical transport in the large subunit of class I ribonucleotide reductase. J Am Chem Soc (2012) 1.04
ENDOR spectroscopy and DFT calculations: evidence for the hydrogen-bond network within α2 in the PCET of E. coli ribonucleotide reductase. J Am Chem Soc (2012) 0.93
Use of 2,3,5-F(3)Y-β2 and 3-NH(2)Y-α2 to study proton-coupled electron transfer in Escherichia coli ribonucleotide reductase. Biochemistry (2011) 0.81
Hydrogen bond network between amino acid radical intermediates on the proton-coupled electron transfer pathway of E. coli α2 ribonucleotide reductase. J Am Chem Soc (2014) 0.80
Radical-translocation intermediates and hurdling of pathway defects in "super-oxidized" (Mn(IV)/Fe(IV)) Chlamydia trachomatis ribonucleotide reductase. J Am Chem Soc (2012) 0.78
EasySpin, a comprehensive software package for spectral simulation and analysis in EPR. J Magn Reson (2005) 5.97
Protein Radicals in Enzyme Catalysis. Chem Rev (1998) 5.03
Radical initiation in the class I ribonucleotide reductase: long-range proton-coupled electron transfer? Chem Rev (2003) 4.45
Ribonucleotide reductases. Annu Rev Biochem (1998) 4.09
Structure of ribonucleotide reductase protein R1. Nature (1994) 3.76
Three-dimensional structure of the free radical protein of ribonucleotide reductase. Nature (1990) 3.24
Expanding the genetic code. Angew Chem Int Ed Engl (2004) 2.78
Ribonucleotide reductase--a radical enzyme. Science (1983) 2.33
Proton-coupled electron transfer: the mechanistic underpinning for radical transport and catalysis in biology. Philos Trans R Soc Lond B Biol Sci (2006) 1.94
Ribonucleoside diphosphate reductase. Purification of the two subunits, proteins B1 and B2. Eur J Biochem (1969) 1.84
Physicochemical characterization of ribonucleoside diphosphate reductase from Escherichia coli. J Biol Chem (1973) 1.79
Thiyl radicals in ribonucleotide reductases. Science (1996) 1.74
The tyrosine free radical in ribonucleotide reductase from Escherichia coli. J Biol Chem (1978) 1.68
Harnessing free radicals: formation and function of the tyrosyl radical in ribonucleotide reductase. Trends Biochem Sci (1998) 1.58
pH Rate profiles of FnY356-R2s (n = 2, 3, 4) in Escherichia coli ribonucleotide reductase: evidence that Y356 is a redox-active amino acid along the radical propagation pathway. J Am Chem Soc (2006) 1.57
Role of effector binding in allosteric control of ribonucleoside diphosphate reductase. J Mol Biol (1969) 1.57
Ribonucleotide reductases: amazing and confusing. J Biol Chem (1990) 1.56
Site-specific insertion of 3-aminotyrosine into subunit alpha2 of E. coli ribonucleotide reductase: direct evidence for involvement of Y730 and Y731 in radical propagation. J Am Chem Soc (2007) 1.54
EPR distance measurements support a model for long-range radical initiation in E. coli ribonucleotide reductase. J Am Chem Soc (2005) 1.52
Electron spin resonance of the iron-containing protein B2 from ribonucleotide reductase. J Biol Chem (1972) 1.51
Site-specific replacement of Y356 with 3,4-dihydroxyphenylalanine in the beta2 subunit of E. coli ribonucleotide reductase. J Am Chem Soc (2006) 1.48
Ribonucleoside diphosphate reductase. Formation of active and inactive complexes of proteins B1 and B2. J Mol Biol (1969) 1.47
Mono-, di-, tri-, and tetra-substituted fluorotyrosines: new probes for enzymes that use tyrosyl radicals in catalysis. J Am Chem Soc (2006) 1.40
A new method of identifying the site of tyrosyl radicals in proteins. Biophys J (2004) 1.36
An expanding genetic code. Methods (2005) 1.35
Generation of the R2 subunit of ribonucleotide reductase by intein chemistry: insertion of 3-nitrotyrosine at residue 356 as a probe of the radical initiation process. Biochemistry (2003) 1.32
PELDOR spectroscopy with DOPA-beta2 and NH2Y-alpha2s: distance measurements between residues involved in the radical propagation pathway of E. coli ribonucleotide reductase. J Am Chem Soc (2007) 1.17
2,3-difluorotyrosine at position 356 of ribonucleotide reductase R2: a probe of long-range proton-coupled electron transfer. J Am Chem Soc (2003) 1.15
High-field EPR detection of a disulfide radical anion in the reduction of cytidine 5'-diphosphate by the E441Q R1 mutant of Escherichia coli ribonucleotide reductase. Proc Natl Acad Sci U S A (1999) 1.13
Photoactive peptides for light-initiated tyrosyl radical generation and transport into ribonucleotide reductase. J Am Chem Soc (2007) 1.11
Electron transfer reactions of fluorotyrosyl radicals. J Am Chem Soc (2006) 1.10
Ribonucleotide reductases in the twenty-first century. Proc Natl Acad Sci U S A (1998) 1.09
Electron transfer between a tyrosyl radical and a cysteine residue in hemoproteins: spin trapping analysis. J Am Chem Soc (2007) 1.08
Pulsed 180-GHz EPR/ENDOR/PELDOR spectroscopy. Magn Reson Chem (2005) 1.08
Forward and reverse electron transfer with the Y356DOPA-beta2 heterodimer of E. coli ribonucleotide reductase. J Am Chem Soc (2007) 1.07
Structure and interactions of amino acid radicals in class I ribonucleotide reductase studied by ENDOR and high-field EPR spectroscopy. Biochim Biophys Acta (2005) 1.05
Tryptophan and tyrosine radicals in ribonucleotide reductase: a comparative high-field EPR study at 94 GHz. Biochemistry (2001) 1.05
High-frequency (140-GHz) time domain EPR and ENDOR spectroscopy: the tyrosyl radical-diiron cofactor in ribonucleotide reductase from yeast. J Am Chem Soc (2001) 1.02
The g-values and hyperfine coupling of amino acid radicals in proteins: comparison of experimental measurements with ab initio calculations. Magn Reson Chem (2005) 1.02
Tyrosyl radical cofactors. Adv Protein Chem (2001) 1.01
Site-specific incorporation of fluorotyrosines into the R2 subunit of E. coli ribonucleotide reductase by expressed protein ligation. Nat Protoc (2007) 0.97
Determination of the structural environment of the tyrosyl radical in prostaglandin H2 synthase-1: a high frequency ENDOR/EPR study. J Am Chem Soc (2005) 0.96
A stable tyrosyl radical in monoamine oxidase A. J Biol Chem (2004) 0.94
High field EPR studies of mouse ribonucleotide reductase indicate hydrogen bonding of the tyrosyl radical. J Biol Chem (1996) 0.94
Evidence for protein radical-mediated nuclear tunneling in fatty acid alpha-oxygenase. J Am Chem Soc (2008) 0.93
Site-specific replacement of a conserved tyrosine in ribonucleotide reductase with an aniline amino acid: a mechanistic probe for a redox-active tyrosine. J Am Chem Soc (2004) 0.93
The ten-stranded beta/alpha barrel in ribonucleotide reductase protein R1. J Mol Biol (1996) 0.91
Tyrosyl radicals in photosystem II. Biochim Biophys Acta (2004) 0.91
Tyrosyl radicals in enzyme catalysis: some properties and a focus on photosynthetic water oxidation. Acta Chem Scand (1997) 0.90
The electronic structure of the Cys-Tyr(*) free radical in galactose oxidase determined by EPR spectroscopy. Biochemistry (2008) 0.90
The function and characteristics of tyrosyl radical cofactors. Biochim Biophys Acta (2004) 0.90
Isolation and characterization of a neutral imino-semiquinone radical. J Am Chem Soc (2008) 0.78
Bleomycins: towards better therapeutics. Nat Rev Cancer (2005) 2.52
The crystal structure of class II ribonucleotide reductase reveals how an allosterically regulated monomer mimics a dimer. Nat Struct Biol (2002) 2.28
Subcellular localization of yeast ribonucleotide reductase regulated by the DNA replication and damage checkpoint pathways. Proc Natl Acad Sci U S A (2003) 2.24
Structural basis for activation of class Ib ribonucleotide reductase. Science (2010) 2.07
Proton-coupled electron transfer: the mechanistic underpinning for radical transport and catalysis in biology. Philos Trans R Soc Lond B Biol Sci (2006) 1.94
Pre-steady-state and steady-state kinetic analysis of E. coli class I ribonucleotide reductase. Biochemistry (2003) 1.68
Enhanced subunit interactions with gemcitabine-5'-diphosphate inhibit ribonucleotide reductases. Proc Natl Acad Sci U S A (2007) 1.63
The Ralstonia eutropha PhaR protein couples synthesis of the PhaP phasin to the presence of polyhydroxybutyrate in cells and promotes polyhydroxybutyrate production. J Bacteriol (2002) 1.60
pH Rate profiles of FnY356-R2s (n = 2, 3, 4) in Escherichia coli ribonucleotide reductase: evidence that Y356 is a redox-active amino acid along the radical propagation pathway. J Am Chem Soc (2006) 1.57
Site-specific insertion of 3-aminotyrosine into subunit alpha2 of E. coli ribonucleotide reductase: direct evidence for involvement of Y730 and Y731 in radical propagation. J Am Chem Soc (2007) 1.54
Ralstonia eutropha H16 encodes two and possibly three intracellular Poly[D-(-)-3-hydroxybutyrate] depolymerase genes. J Bacteriol (2003) 1.46
Mono-, di-, tri-, and tetra-substituted fluorotyrosines: new probes for enzymes that use tyrosyl radicals in catalysis. J Am Chem Soc (2006) 1.40
Generation of the R2 subunit of ribonucleotide reductase by intein chemistry: insertion of 3-nitrotyrosine at residue 356 as a probe of the radical initiation process. Biochemistry (2003) 1.32
Structure of the nucleotide radical formed during reaction of CDP/TTP with the E441Q-alpha2beta2 of E. coli ribonucleotide reductase. J Am Chem Soc (2009) 1.32
YfaE, a ferredoxin involved in diferric-tyrosyl radical maintenance in Escherichia coli ribonucleotide reductase. Biochemistry (2007) 1.30
NrdI, a flavodoxin involved in maintenance of the diferric-tyrosyl radical cofactor in Escherichia coli class Ib ribonucleotide reductase. Proc Natl Acad Sci U S A (2008) 1.23
Mechanism of inactivation of human ribonucleotide reductase with p53R2 by gemcitabine 5'-diphosphate. Biochemistry (2009) 1.18
PELDOR spectroscopy with DOPA-beta2 and NH2Y-alpha2s: distance measurements between residues involved in the radical propagation pathway of E. coli ribonucleotide reductase. J Am Chem Soc (2007) 1.17
Nuclear localization of the Saccharomyces cerevisiae ribonucleotide reductase small subunit requires a karyopherin and a WD40 repeat protein. Proc Natl Acad Sci U S A (2006) 1.17
2,3-difluorotyrosine at position 356 of ribonucleotide reductase R2: a probe of long-range proton-coupled electron transfer. J Am Chem Soc (2003) 1.15
Structures of the yeast ribonucleotide reductase Rnr2 and Rnr4 homodimers. Biochemistry (2004) 1.13
A hot oxidant, 3-NO2Y122 radical, unmasks conformational gating in ribonucleotide reductase. J Am Chem Soc (2010) 1.12
Heterodisulfide reductase from methanogenic archaea: a new catalytic role for an iron-sulfur cluster. Biol Chem (2005) 1.12
A cysteine-rich CCG domain contains a novel [4Fe-4S] cluster binding motif as deduced from studies with subunit B of heterodisulfide reductase from Methanothermobacter marburgensis. Biochemistry (2007) 1.12
Electron transfer reactions of fluorotyrosyl radicals. J Am Chem Soc (2006) 1.10
The active form of the Saccharomyces cerevisiae ribonucleotide reductase small subunit is a heterodimer in vitro and in vivo. Biochemistry (2005) 1.10
Direct observation of a transient tyrosine radical competent for initiating turnover in a photochemical ribonucleotide reductase. J Am Chem Soc (2007) 1.09
Kinetics of radical intermediate formation and deoxynucleotide production in 3-aminotyrosine-substituted Escherichia coli ribonucleotide reductases. J Am Chem Soc (2011) 1.09
Chris Raetz, scientist and enduring friend. Proc Natl Acad Sci U S A (2011) 1.08
Forward and reverse electron transfer with the Y356DOPA-beta2 heterodimer of E. coli ribonucleotide reductase. J Am Chem Soc (2007) 1.07
Turning on ribonucleotide reductase by light-initiated amino acid radical generation. Proc Natl Acad Sci U S A (2004) 1.06
Mechanistic studies on bleomycin-mediated DNA damage: multiple binding modes can result in double-stranded DNA cleavage. Nucleic Acids Res (2008) 1.05
Synthesis and characterization of oligonucleotides containing a 4'-keto abasic site. Biochemistry (2004) 1.05
Insight into the mechanism of inactivation of ribonucleotide reductase by gemcitabine 5'-diphosphate in the presence or absence of reductant. Biochemistry (2009) 1.04
Importance of the maintenance pathway in the regulation of the activity of Escherichia coli ribonucleotide reductase. Biochemistry (2008) 1.04
Investigation of in vivo diferric tyrosyl radical formation in Saccharomyces cerevisiae Rnr2 protein: requirement of Rnr4 and contribution of Grx3/4 AND Dre2 proteins. J Biol Chem (2011) 1.03
A dual-mode microwave resonator for double electron-electron spin resonance spectroscopy at W-band microwave frequencies. J Magn Reson (2011) 1.03
Domain organization of Salmonella typhimurium formylglycinamide ribonucleotide amidotransferase revealed by X-ray crystallography. Biochemistry (2004) 1.02
Field dependent dynamic nuclear polarization with radicals in aqueous solution. J Am Chem Soc (2008) 1.01
Complexed structures of formylglycinamide ribonucleotide amidotransferase from Thermotoga maritima describe a novel ATP binding protein superfamily. Biochemistry (2006) 1.01
Structure of the nitrogen-centered radical formed during inactivation of E. coli ribonucleotide reductase by 2'-azido-2'-deoxyuridine-5'-diphosphate: trapping of the 3'-ketonucleotide. J Am Chem Soc (2005) 0.99
Incorporation of fluorotyrosines into ribonucleotide reductase using an evolved, polyspecific aminoacyl-tRNA synthetase. J Am Chem Soc (2011) 0.98
Purification of polyhydroxybutyrate synthase from its native organism, Ralstonia eutropha: implications for the initiation and elongation of polymer formation in vivo. Biochemistry (2012) 0.98
Site-specific incorporation of fluorotyrosines into the R2 subunit of E. coli ribonucleotide reductase by expressed protein ligation. Nat Protoc (2007) 0.97
Growth and localization of polyhydroxybutyrate granules in Ralstonia eutropha. J Bacteriol (2011) 0.96
Physicochemical and formulation developability assessment for therapeutic peptide delivery--a primer. AAPS J (2014) 0.96
Solution structure of the hydroperoxide of Co(III) phleomycin complexed with d(CCAGGCCTGG)2: evidence for binding by partial intercalation. Nucleic Acids Res (2002) 0.95
Detection of intermediates from the polymerization reaction catalyzed by a D302A mutant of class III polyhydroxyalkanoate (PHA) synthase. Biochemistry (2005) 0.95
GC/MS methods to quantify the 2-deoxypentos-4-ulose and 3'-phosphoglycolate pathways of 4' oxidation of 2-deoxyribose in DNA: application to DNA damage produced by gamma radiation and bleomycin. Chem Res Toxicol (2007) 0.95
Shuttle DNP spectrometer with a two-center magnet. Phys Chem Chem Phys (2010) 0.95
Methodology to probe subunit interactions in ribonucleotide reductases. Biochemistry (2008) 0.94
Determination of the in vivo stoichiometry of tyrosyl radical per betabeta' in Saccharomyces cerevisiae ribonucleotide reductase. Biochemistry (2006) 0.94
Use of 3-aminotyrosine to examine the pathway dependence of radical propagation in Escherichia coli ribonucleotide reductase. Biochemistry (2009) 0.94
Function of the diiron cluster of Escherichia coli class Ia ribonucleotide reductase in proton-coupled electron transfer. J Am Chem Soc (2013) 0.94
(1)H and (13)C dynamic nuclear polarization in aqueous solution with a two-field (0.35 T/14 T) shuttle DNP spectrometer. J Am Chem Soc (2009) 0.94
Mechanistic studies of semicarbazone triapine targeting human ribonucleotide reductase in vitro and in mammalian cells: tyrosyl radical quenching not involving reactive oxygen species. J Biol Chem (2012) 0.94
ENDOR spectroscopy and DFT calculations: evidence for the hydrogen-bond network within α2 in the PCET of E. coli ribonucleotide reductase. J Am Chem Soc (2012) 0.93
Protein Radicals in Enzyme Catalysis. [Chem. Rev. 1998, 98, 705minus sign762. Chem Rev (1998) 0.93
Site-specific replacement of a conserved tyrosine in ribonucleotide reductase with an aniline amino acid: a mechanistic probe for a redox-active tyrosine. J Am Chem Soc (2004) 0.93
Photo-ribonucleotide reductase β2 by selective cysteine labeling with a radical phototrigger. Proc Natl Acad Sci U S A (2011) 0.93
Mechanism of assembly of the dimanganese-tyrosyl radical cofactor of class Ib ribonucleotide reductase: enzymatic generation of superoxide is required for tyrosine oxidation via a Mn(III)Mn(IV) intermediate. J Am Chem Soc (2013) 0.91
The formylglycinamide ribonucleotide amidotransferase complex from Bacillus subtilis: metabolite-mediated complex formation. Biochemistry (2004) 0.91
Pulsed ELDOR spectroscopy measures the distance between the two tyrosyl dadicals in the R2 subunit of the E. coli ribonucleotide reductase. J Am Chem Soc (2003) 0.91
Re(bpy)(CO)3CN as a probe of conformational flexibility in a photochemical ribonucleotide reductase. Biochemistry (2009) 0.91
Probing secondary structures of spin-labeled RNA by pulsed EPR spectroscopy. Angew Chem Int Ed Engl (2010) 0.90
Saturation factor of nitroxide radicals in liquid DNP by pulsed ELDOR experiments. Phys Chem Chem Phys (2011) 0.90
Orientation selection in distance measurements between nitroxide spin labels at 94 GHz EPR with variable dual frequency irradiation. Phys Chem Chem Phys (2013) 0.90
Class III polyhydroxybutyrate synthase: involvement in chain termination and reinitiation. Biochemistry (2005) 0.90
N5-CAIR mutase: role of a CO2 binding site and substrate movement in catalysis. Biochemistry (2007) 0.90
Nuclear magnetic resonance structural studies and molecular modeling of duplex DNA containing normal and 4'-oxidized abasic sites. Biochemistry (2007) 0.89
A model for the Bacillus subtilis formylglycinamide ribonucleotide amidotransferase multiprotein complex. Biochemistry (2004) 0.89
Mechanistic insight with HBCH2CoA as a probe to polyhydroxybutyrate (PHB) synthases. ACS Chem Biol (2014) 0.88
Clofarabine targets the large subunit (α) of human ribonucleotide reductase in live cells by assembly into persistent hexamers. Chem Biol (2012) 0.87
Epimerization at carbon-5' of (5'R)-[5'-2H]adenosylcobalamin by ribonucleoside triphosphate reductase: cysteine 408-independent cleavage of the Co-C5' bond. Biochemistry (2003) 0.87
Optimization of dynamic nuclear polarization experiments in aqueous solution at 15 MHz/9.7 GHz: a comparative study with DNP at 140 MHz/94 GHz. Phys Chem Chem Phys (2010) 0.86
Conserved electron donor complex Dre2-Tah18 is required for ribonucleotide reductase metallocofactor assembly and DNA synthesis. Proc Natl Acad Sci U S A (2014) 0.86
Formal reduction potential of 3,5-difluorotyrosine in a structured protein: insight into multistep radical transfer. Biochemistry (2013) 0.85
Structure of the tyrosyl biradical in mouse R2 ribonucleotide reductase from high-field PELDOR. Angew Chem Int Ed Engl (2008) 0.85
W-band orientation selective DEER measurements on a Gd3+/nitroxide mixed-labeled protein dimer with a dual mode cavity. J Magn Reson (2012) 0.85
Streptococcus sanguinis class Ib ribonucleotide reductase: high activity with both iron and manganese cofactors and structural insights. J Biol Chem (2013) 0.85
A chemically competent thiosulfuranyl radical on the Escherichia coli class III ribonucleotide reductase. J Am Chem Soc (2014) 0.85
Formylglycinamide ribonucleotide amidotransferase from Thermotoga maritima: structural insights into complex formation. Biochemistry (2008) 0.84
Detection of covalent and noncovalent intermediates in the polymerization reaction catalyzed by a C149S class III polyhydroxybutyrate synthase. Biochemistry (2009) 0.84
Electron paramagnetic resonance spectroscopy measures the distance between the external β-strands of folded α-synuclein in amyloid fibrils. Biophys J (2011) 0.83
Synthesis, characterization and solution structure of tethered oligonucleotides containing an internal 3'-phosphoglycolate, 5'-phosphate gapped lesion. Nucleic Acids Res (2002) 0.83
Conformational states of human H-Ras detected by high-field EPR, ENDOR, and 31P NMR spectroscopy. Magn Reson Chem (2005) 0.83
Redox-linked changes to the hydrogen-bonding network of ribonucleotide reductase β2. J Am Chem Soc (2013) 0.83
Kinetics of hydrogen atom abstraction from substrate by an active site thiyl radical in ribonucleotide reductase. J Am Chem Soc (2014) 0.83
Overhauser DNP with 15N labelled Frémy's salt at 0.35 Tesla. Phys Chem Chem Phys (2011) 0.83
Genetic characterization and role in virulence of the ribonucleotide reductases of Streptococcus sanguinis. J Biol Chem (2013) 0.82
Multifrequency electron paramagnetic resonance characterization of PpoA, a CYP450 fusion protein that catalyzes fatty acid dioxygenation. J Am Chem Soc (2011) 0.82
Use of 2,3,5-F(3)Y-β2 and 3-NH(2)Y-α2 to study proton-coupled electron transfer in Escherichia coli ribonucleotide reductase. Biochemistry (2011) 0.81
Phosphate triester hydrolysis promoted by an N2S(thiolate)Zn complex: mechanistic implications for the metal-dependent reactivity of peptide deformylase. Inorg Chem (2005) 0.81