Published in J Bacteriol on December 16, 2011
Localization of poly(3-hydroxybutyrate) (PHB) granule-associated proteins during PHB granule formation and identification of two new phasins, PhaP6 and PhaP7, in Ralstonia eutropha H16. J Bacteriol (2012) 1.08
Purification of polyhydroxybutyrate synthase from its native organism, Ralstonia eutropha: implications for the initiation and elongation of polymer formation in vivo. Biochemistry (2012) 0.98
Comparative proteome analysis reveals four novel polyhydroxybutyrate (PHB) granule-associated proteins in Ralstonia eutropha H16. Appl Environ Microbiol (2014) 0.89
PHB granules are attached to the nucleoid via PhaM in Ralstonia eutropha. BMC Microbiol (2012) 0.88
PhaM is the physiological activator of poly(3-hydroxybutyrate) (PHB) synthase (PhaC1) in Ralstonia eutropha. Appl Environ Microbiol (2013) 0.85
A new view into prokaryotic cell biology from electron cryotomography. Nat Rev Microbiol (2016) 0.84
Identification of proteins associated with polyhydroxybutyrate granules from Herbaspirillum seropedicae SmR1--old partners, new players. PLoS One (2013) 0.84
Formation of Polyphosphate by Polyphosphate Kinases and Its Relationship to Poly(3-Hydroxybutyrate) Accumulation in Ralstonia eutropha Strain H16. Appl Environ Microbiol (2015) 0.84
Development of a transferable bimolecular fluorescence complementation system for the investigation of interactions between poly(3-hydroxybutyrate) granule-associated proteins in Gram-negative bacteria. Appl Environ Microbiol (2013) 0.82
Ultrastructure and complex polar architecture of the human pathogen Campylobacter jejuni. Microbiologyopen (2014) 0.81
Evaluation of a multicore-optimized implementation for tomographic reconstruction. PLoS One (2012) 0.77
Polyhydroxyalkanoate (PHA) Granules Have no Phospholipids. Sci Rep (2016) 0.77
Proteomic phenotyping of Novosphingobium nitrogenifigens reveals a robust capacity for simultaneous nitrogen fixation, polyhydroxyalkanoate production, and resistance to reactive oxygen species. Appl Environ Microbiol (2012) 0.76
Proteins with CHADs (Conserved Histidine α-Helical Domains) Are Attached to Polyphosphate Granules In Vivo and Constitute a Novel Family of Polyphosphate-Associated Proteins (Phosins). Appl Environ Microbiol (2017) 0.75
Computer visualization of three-dimensional image data using IMOD. J Struct Biol (1996) 25.42
Magnetosomes are cell membrane invaginations organized by the actin-like protein MamK. Science (2005) 4.19
Poly-beta-hydroxybutyrate (PHB) biosynthesis in Alcaligenes eutrophus H16. Identification and characterization of the PHB polymerase gene (phbC). J Biol Chem (1989) 3.79
The structure of FtsZ filaments in vivo suggests a force-generating role in cell division. EMBO J (2007) 3.44
Universal architecture of bacterial chemoreceptor arrays. Proc Natl Acad Sci U S A (2009) 2.50
Analysis of a 24-kilodalton protein associated with the polyhydroxyalkanoic acid granules in Alcaligenes eutrophus. J Bacteriol (1995) 2.41
Quantitative genome-scale analysis of protein localization in an asymmetric bacterium. Proc Natl Acad Sci U S A (2009) 2.33
Spatially ordered dynamics of the bacterial carbon fixation machinery. Science (2010) 2.22
Overexpression and purification of the soluble polyhydroxyalkanoate synthase from Alcaligenes eutrophus: evidence for a required posttranslational modification for catalytic activity. Biochemistry (1994) 1.98
Metabolism of poly-beta-hydroxybutyrate. I. Purification, composition, and properties of native poly-beta-hydroxybutyrate granules from Bacillus megaterium. Biochemistry (1968) 1.93
Fast tomographic reconstruction on multicore computers. Bioinformatics (2010) 1.89
Fully automated, sequential tilt-series acquisition with Leginon. J Struct Biol (2009) 1.84
Markov random field based automatic image alignment for electron tomography. J Struct Biol (2007) 1.75
Accumulation of the PhaP phasin of Ralstonia eutropha is dependent on production of polyhydroxybutyrate in cells. J Bacteriol (2001) 1.70
Cloning of an intracellular Poly[D(-)-3-Hydroxybutyrate] depolymerase gene from Ralstonia eutropha H16 and characterization of the gene product. J Bacteriol (2001) 1.70
New insight into the role of the PhaP phasin of Ralstonia eutropha in promoting synthesis of polyhydroxybutyrate. J Bacteriol (2001) 1.69
Regulation of phasin expression and polyhydroxyalkanoate (PHA) granule formation in Ralstonia eutropha H16. Microbiology (2002) 1.68
Synthesis, storage and degradation of neutral lipids in yeast. Biochim Biophys Acta (2006) 1.64
Structural diversity of bacterial flagellar motors. EMBO J (2011) 1.63
Bacterial chromatin. Curr Opin Genet Dev (2005) 1.63
Polyhydroxyalkanoate granules are complex subcellular organelles (carbonosomes). J Bacteriol (2009) 1.60
The Ralstonia eutropha PhaR protein couples synthesis of the PhaP phasin to the presence of polyhydroxybutyrate in cells and promotes polyhydroxybutyrate production. J Bacteriol (2002) 1.60
Nontemplate-dependent polymerization processes: polyhydroxyalkanoate synthases as a paradigm. Annu Rev Biochem (2005) 1.45
Polyhydroxyalkanoate (PHA) hemeostasis: the role of PHA synthase. Nat Prod Rep (2003) 1.45
In vivo monitoring of PHA granule formation using GFP-labeled PHA synthases. FEMS Microbiol Lett (2005) 1.38
Polyhydroxyalkanoates: bioplastics with a green agenda. Curr Opin Microbiol (2010) 1.36
E. coli transports aggregated proteins to the poles by a specific and energy-dependent process. J Mol Biol (2009) 1.34
Electron cryotomography: a new view into microbial ultrastructure. Curr Opin Microbiol (2009) 1.26
Immunocytochemical analysis of poly-beta-hydroxybutyrate (PHB) synthase in Alcaligenes eutrophus H16: localization of the synthase enzyme at the surface of PHB granules. J Bacteriol (1993) 1.25
Kinetic studies of polyhydroxybutyrate granule formation in Wautersia eutropha H16 by transmission electron microscopy. J Bacteriol (2005) 1.24
Nucleoid-associated PhaF phasin drives intracellular location and segregation of polyhydroxyalkanoate granules in Pseudomonas putida KT2442. Mol Microbiol (2010) 1.24
Enzyme-catalyzed synthesis of poly[(R)-(-)-3-hydroxybutyrate]: formation of macroscopic granules in vitro. Proc Natl Acad Sci U S A (1995) 1.21
Elucidation of beta-oxidation pathways in Ralstonia eutropha H16 by examination of global gene expression. J Bacteriol (2010) 1.18
Bacterial TEM: new insights from cryo-microscopy. Methods Cell Biol (2010) 1.17
Identification of a multifunctional protein, PhaM, that determines number, surface to volume ratio, subcellular localization and distribution to daughter cells of poly(3-hydroxybutyrate), PHB, granules in Ralstonia eutropha H16. Mol Microbiol (2011) 1.16
Analysis of transient polyhydroxybutyrate production in Wautersia eutropha H16 by quantitative Western analysis and transmission electron microscopy. J Bacteriol (2005) 1.14
Binding of the major phasin, PhaP1, from Ralstonia eutropha H16 to poly(3-hydroxybutyrate) granules. J Bacteriol (2008) 1.12
Detection and characterization of protein aggregates by fluorescence microscopy. Int J Pharm (2006) 1.09
Polar explorations Recent insights into the polarity of bacterial proteins. Curr Opin Microbiol (2007) 1.05
Characterization of intact subcellular bodies in whole bacteria by cryo-electron tomography and spectroscopic imaging. J Microsc (2006) 1.05
Poly(3-hydroxybutyrate) granules at the early stages of formation are localized close to the cytoplasmic membrane in Caryophanon latum. Appl Environ Microbiol (2006) 1.03
Interaction between poly(3-hydroxybutyrate) granule-associated proteins as revealed by two-hybrid analysis and identification of a new phasin in Ralstonia eutropha H16. Microbiology (2011) 1.03
Transcriptional analysis of Ralstonia eutropha genes related to poly-(R)-3-hydroxybutyrate homeostasis during batch fermentation. Appl Microbiol Biotechnol (2005) 1.01
Fluorescence microscopical investigation of poly(3-hydroxybutyrate) granule formation in bacteria. Biomacromolecules (2005) 0.98
The inherent property of polyhydroxyalkanoate synthase to form spherical PHA granules at the cell poles: the core region is required for polar localization. J Biotechnol (2007) 0.94
Exploiting genomic patterns to discover new supramolecular protein assemblies. Protein Sci (2009) 0.92
Spiral architecture of the nucleoid in Bdellovibrio bacteriovorus. J Bacteriol (2010) 0.91
Microscopical investigation of poly(3-hydroxybutyrate) granule formation in Azotobacter vinelandii. FEMS Microbiol Lett (2007) 0.90
Class III polyhydroxybutyrate synthase: involvement in chain termination and reinitiation. Biochemistry (2005) 0.90
PhaP is involved in the formation of a network on the surface of polyhydroxyalkanoate inclusions in Cupriavidus necator H16. J Bacteriol (2007) 0.81
Atomic force microscopic observation of in vitro polymerized poly[(R)-3-hydroxybutyrate]: insight into possible mechanism of granule formation. Biomacromolecules (2005) 0.78
Nuclear magnetic resonance relaxation studies of poly(hydroxybutyrate) in whole cells and in artificial granules. Int J Biol Macromol (1994) 0.77
Magnetosomes are cell membrane invaginations organized by the actin-like protein MamK. Science (2005) 4.19
The structure of FtsZ filaments in vivo suggests a force-generating role in cell division. EMBO J (2007) 3.44
Location and architecture of the Caulobacter crescentus chemoreceptor array. Mol Microbiol (2008) 3.41
Electron cryotomography of immature HIV-1 virions reveals the structure of the CA and SP1 Gag shells. EMBO J (2007) 3.17
A self-associating protein critical for chromosome attachment, division, and polar organization in caulobacter. Cell (2008) 3.02
Multiple large filament bundles observed in Caulobacter crescentus by electron cryotomography. Mol Microbiol (2006) 2.59
Bleomycins: towards better therapeutics. Nat Rev Cancer (2005) 2.52
Universal architecture of bacterial chemoreceptor arrays. Proc Natl Acad Sci U S A (2009) 2.50
General protein diffusion barriers create compartments within bacterial cells. Cell (2012) 2.48
Molecular organization of Gram-negative peptidoglycan. Proc Natl Acad Sci U S A (2008) 2.33
The crystal structure of class II ribonucleotide reductase reveals how an allosterically regulated monomer mimics a dimer. Nat Struct Biol (2002) 2.28
Subcellular localization of yeast ribonucleotide reductase regulated by the DNA replication and damage checkpoint pathways. Proc Natl Acad Sci U S A (2003) 2.24
Three-dimensional structure of HIV-1 virus-like particles by electron cryotomography. J Mol Biol (2004) 2.10
Structural basis for activation of class Ib ribonucleotide reductase. Science (2010) 2.07
The metabolic enzyme CTP synthase forms cytoskeletal filaments. Nat Cell Biol (2010) 2.01
Proton-coupled electron transfer: the mechanistic underpinning for radical transport and catalysis in biology. Philos Trans R Soc Lond B Biol Sci (2006) 1.94
Bacterial chemoreceptor arrays are hexagonally packed trimers of receptor dimers networked by rings of kinase and coupling proteins. Proc Natl Acad Sci U S A (2012) 1.89
Fully automated, sequential tilt-series acquisition with Leginon. J Struct Biol (2009) 1.84
Electron cryotomography sample preparation using the Vitrobot. Nat Protoc (2006) 1.83
An improved cryogen for plunge freezing. Microsc Microanal (2008) 1.82
In situ structure of the complete Treponema primitia flagellar motor. Nature (2006) 1.82
3-D ultrastructure of O. tauri: electron cryotomography of an entire eukaryotic cell. PLoS One (2007) 1.72
Pre-steady-state and steady-state kinetic analysis of E. coli class I ribonucleotide reductase. Biochemistry (2003) 1.68
Structural diversity of bacterial flagellar motors. EMBO J (2011) 1.63
Enhanced subunit interactions with gemcitabine-5'-diphosphate inhibit ribonucleotide reductases. Proc Natl Acad Sci U S A (2007) 1.63
The structure of isolated Synechococcus strain WH8102 carboxysomes as revealed by electron cryotomography. J Mol Biol (2007) 1.63
Novel ultrastructures of Treponema primitia and their implications for motility. Mol Microbiol (2008) 1.63
FcRn-mediated antibody transport across epithelial cells revealed by electron tomography. Nature (2008) 1.62
The Ralstonia eutropha PhaR protein couples synthesis of the PhaP phasin to the presence of polyhydroxybutyrate in cells and promotes polyhydroxybutyrate production. J Bacteriol (2002) 1.60
The helical MreB cytoskeleton in Escherichia coli MC1000/pLE7 is an artifact of the N-Terminal yellow fluorescent protein tag. J Bacteriol (2012) 1.58
pH Rate profiles of FnY356-R2s (n = 2, 3, 4) in Escherichia coli ribonucleotide reductase: evidence that Y356 is a redox-active amino acid along the radical propagation pathway. J Am Chem Soc (2006) 1.57
Site-specific insertion of 3-aminotyrosine into subunit alpha2 of E. coli ribonucleotide reductase: direct evidence for involvement of Y730 and Y731 in radical propagation. J Am Chem Soc (2007) 1.54
Radiation dose reduction and image enhancement in biological imaging through equally-sloped tomography. J Struct Biol (2008) 1.52
DipM, a new factor required for peptidoglycan remodelling during cell division in Caulobacter crescentus. Mol Microbiol (2010) 1.52
Organization, structure, and assembly of alpha-carboxysomes determined by electron cryotomography of intact cells. J Mol Biol (2009) 1.50
Toward a biomechanical understanding of whole bacterial cells. Annu Rev Biochem (2008) 1.49
A comparison of liquid nitrogen and liquid helium as cryogens for electron cryotomography. J Struct Biol (2006) 1.47
Ralstonia eutropha H16 encodes two and possibly three intracellular Poly[D-(-)-3-hydroxybutyrate] depolymerase genes. J Bacteriol (2003) 1.46
Observations on the behavior of vitreous ice at approximately 82 and approximately 12 K. J Struct Biol (2006) 1.41
Peptidoglycan transformations during Bacillus subtilis sporulation. Mol Microbiol (2013) 1.38
Electron tomography of cells. Q Rev Biophys (2011) 1.37
Peptidoglycan remodeling and conversion of an inner membrane into an outer membrane during sporulation. Cell (2011) 1.37
Three-dimensional structure of Mycoplasma pneumoniae's attachment organelle and a model for its role in gliding motility. Mol Microbiol (2006) 1.36
Long helical filaments are not seen encircling cells in electron cryotomograms of rod-shaped bacteria. Biochem Biophys Res Commun (2011) 1.35
Architecture and assembly of the Gram-positive cell wall. Mol Microbiol (2013) 1.34
Cryo-EM structure of dodecameric Vps4p and its 2:1 complex with Vta1p. J Mol Biol (2008) 1.33
Generation of the R2 subunit of ribonucleotide reductase by intein chemistry: insertion of 3-nitrotyrosine at residue 356 as a probe of the radical initiation process. Biochemistry (2003) 1.32
YfaE, a ferredoxin involved in diferric-tyrosyl radical maintenance in Escherichia coli ribonucleotide reductase. Biochemistry (2007) 1.30
A multidomain hub anchors the chromosome segregation and chemotactic machinery to the bacterial pole. Genes Dev (2012) 1.29
Electron cryotomography: a new view into microbial ultrastructure. Curr Opin Microbiol (2009) 1.26
Architecture of the major component of the type III secretion system export apparatus. Nat Struct Mol Biol (2012) 1.24
Bactofilins, a ubiquitous class of cytoskeletal proteins mediating polar localization of a cell wall synthase in Caulobacter crescentus. EMBO J (2009) 1.23
NrdI, a flavodoxin involved in maintenance of the diferric-tyrosyl radical cofactor in Escherichia coli class Ib ribonucleotide reductase. Proc Natl Acad Sci U S A (2008) 1.23
Rigid, specific, and discrete gold nanoparticle/antibody conjugates. J Am Chem Soc (2006) 1.22
Data management challenges in three-dimensional EM. Nat Struct Mol Biol (2012) 1.19
Mechanism of inactivation of human ribonucleotide reductase with p53R2 by gemcitabine 5'-diphosphate. Biochemistry (2009) 1.18
A "flip-flop" rotation stage for routine dual-axis electron cryotomography. J Struct Biol (2005) 1.17
Nuclear localization of the Saccharomyces cerevisiae ribonucleotide reductase small subunit requires a karyopherin and a WD40 repeat protein. Proc Natl Acad Sci U S A (2006) 1.17
PELDOR spectroscopy with DOPA-beta2 and NH2Y-alpha2s: distance measurements between residues involved in the radical propagation pathway of E. coli ribonucleotide reductase. J Am Chem Soc (2007) 1.17
Bacterial TEM: new insights from cryo-microscopy. Methods Cell Biol (2010) 1.17
Alternative mechanism for bacteriophage adsorption to the motile bacterium Caulobacter crescentus. Proc Natl Acad Sci U S A (2011) 1.17
2,3-difluorotyrosine at position 356 of ribonucleotide reductase R2: a probe of long-range proton-coupled electron transfer. J Am Chem Soc (2003) 1.15
Marine tubeworm metamorphosis induced by arrays of bacterial phage tail-like structures. Science (2014) 1.15
Microtubules in bacteria: Ancient tubulins build a five-protofilament homolog of the eukaryotic cytoskeleton. PLoS Biol (2011) 1.14
Structures of the yeast ribonucleotide reductase Rnr2 and Rnr4 homodimers. Biochemistry (2004) 1.13
Electron cryotomography. Cold Spring Harb Perspect Biol (2010) 1.12
In vivo assembly of an archaeal virus studied with whole-cell electron cryotomography. Structure (2010) 1.12
Correlated light and electron cryo-microscopy. Methods Enzymol (2010) 1.12
A hot oxidant, 3-NO2Y122 radical, unmasks conformational gating in ribonucleotide reductase. J Am Chem Soc (2010) 1.12
Electron transfer reactions of fluorotyrosyl radicals. J Am Chem Soc (2006) 1.10
The active form of the Saccharomyces cerevisiae ribonucleotide reductase small subunit is a heterodimer in vitro and in vivo. Biochemistry (2005) 1.10
Direct observation of a transient tyrosine radical competent for initiating turnover in a photochemical ribonucleotide reductase. J Am Chem Soc (2007) 1.09
Kinetics of radical intermediate formation and deoxynucleotide production in 3-aminotyrosine-substituted Escherichia coli ribonucleotide reductases. J Am Chem Soc (2011) 1.09
Chris Raetz, scientist and enduring friend. Proc Natl Acad Sci U S A (2011) 1.08
Unclosed HIV-1 capsids suggest a curled sheet model of assembly. J Mol Biol (2012) 1.08
Forward and reverse electron transfer with the Y356DOPA-beta2 heterodimer of E. coli ribonucleotide reductase. J Am Chem Soc (2007) 1.07
Plunge freezing for electron cryomicroscopy. Methods Enzymol (2010) 1.06
Turning on ribonucleotide reductase by light-initiated amino acid radical generation. Proc Natl Acad Sci U S A (2004) 1.06
Synthesis and characterization of oligonucleotides containing a 4'-keto abasic site. Biochemistry (2004) 1.05
Mechanistic studies on bleomycin-mediated DNA damage: multiple binding modes can result in double-stranded DNA cleavage. Nucleic Acids Res (2008) 1.05
Cryo-electron tomography of homophilic adhesion mediated by the neural cell adhesion molecule L1. Structure (2009) 1.05
Insight into the mechanism of inactivation of ribonucleotide reductase by gemcitabine 5'-diphosphate in the presence or absence of reductant. Biochemistry (2009) 1.04
Importance of the maintenance pathway in the regulation of the activity of Escherichia coli ribonucleotide reductase. Biochemistry (2008) 1.04
Investigation of in vivo diferric tyrosyl radical formation in Saccharomyces cerevisiae Rnr2 protein: requirement of Rnr4 and contribution of Grx3/4 AND Dre2 proteins. J Biol Chem (2011) 1.03
Structure of bacterial cytoplasmic chemoreceptor arrays and implications for chemotactic signaling. Elife (2014) 1.03
Nanopods: a new bacterial structure and mechanism for deployment of outer membrane vesicles. PLoS One (2011) 1.03
Domain organization of Salmonella typhimurium formylglycinamide ribonucleotide amidotransferase revealed by X-ray crystallography. Biochemistry (2004) 1.02
Electron cryotomography of ESCRT assemblies and dividing Sulfolobus cells suggests that spiraling filaments are involved in membrane scission. Mol Biol Cell (2013) 1.01
The mobility of two kinase domains in the Escherichia coli chemoreceptor array varies with signalling state. Mol Microbiol (2013) 1.01
Complexed structures of formylglycinamide ribonucleotide amidotransferase from Thermotoga maritima describe a novel ATP binding protein superfamily. Biochemistry (2006) 1.01
Activated chemoreceptor arrays remain intact and hexagonally packed. Mol Microbiol (2011) 1.00
Electron cryotomography. Biotechniques (2007) 1.00
Structure of the nitrogen-centered radical formed during inactivation of E. coli ribonucleotide reductase by 2'-azido-2'-deoxyuridine-5'-diphosphate: trapping of the 3'-ketonucleotide. J Am Chem Soc (2005) 0.99
Purification of polyhydroxybutyrate synthase from its native organism, Ralstonia eutropha: implications for the initiation and elongation of polymer formation in vivo. Biochemistry (2012) 0.98
Incorporation of fluorotyrosines into ribonucleotide reductase using an evolved, polyspecific aminoacyl-tRNA synthetase. J Am Chem Soc (2011) 0.98
Structural examination of the transient 3-aminotyrosyl radical on the PCET pathway of E. coli ribonucleotide reductase by multifrequency EPR spectroscopy. J Am Chem Soc (2009) 0.98
The bacterial cytoskeleton: more than twisted filaments. Curr Opin Cell Biol (2012) 0.96
Solution structure of the hydroperoxide of Co(III) phleomycin complexed with d(CCAGGCCTGG)2: evidence for binding by partial intercalation. Nucleic Acids Res (2002) 0.95
Detection of intermediates from the polymerization reaction catalyzed by a D302A mutant of class III polyhydroxyalkanoate (PHA) synthase. Biochemistry (2005) 0.95
GC/MS methods to quantify the 2-deoxypentos-4-ulose and 3'-phosphoglycolate pathways of 4' oxidation of 2-deoxyribose in DNA: application to DNA damage produced by gamma radiation and bleomycin. Chem Res Toxicol (2007) 0.95