Published in Protein Eng Des Sel on February 03, 2010
MF59 adjuvant enhances diversity and affinity of antibody-mediated immune response to pandemic influenza vaccines. Sci Transl Med (2011) 2.75
A designed ankyrin repeat protein selected to bind to tubulin caps the microtubule plus end. Proc Natl Acad Sci U S A (2012) 1.16
Mapping protein interactions by combining antibody affinity maturation and mass spectrometry. Anal Biochem (2011) 0.96
Her2-specific multivalent adapters confer designed tropism to adenovirus for gene targeting. J Mol Biol (2010) 0.89
CDR-restricted engineering of native human scFvs creates highly stable and soluble bifunctional antibodies for subcutaneous delivery. MAbs (2013) 0.87
Conformation-dependent recognition of HIV gp120 by designed ankyrin repeat proteins provides access to novel HIV entry inhibitors. J Virol (2013) 0.82
Conceptual and methodological advances in cell-free directed evolution. Curr Opin Struct Biol (2015) 0.77
Multivalent display of minimal Clostridium difficile glycan epitopes mimics antigenic properties of larger glycans. Nat Commun (2016) 0.75
Destabilizing an interacting motif strengthens the association of a designed ankyrin repeat protein with tubulin. Sci Rep (2016) 0.75
Streamlining the Pipeline for Generation of Recombinant Affinity Reagents by Integrating the Affinity Maturation Step. Int J Mol Sci (2015) 0.75
Engineering novel binding proteins from nonimmunoglobulin domains. Nat Biotechnol (2005) 3.67
High-affinity binders selected from designed ankyrin repeat protein libraries. Nat Biotechnol (2004) 3.34
Designing repeat proteins: well-expressed, soluble and stable proteins from combinatorial libraries of consensus ankyrin repeat proteins. J Mol Biol (2003) 2.81
Signal sequences directing cotranslational translocation expand the range of proteins amenable to phage display. Nat Biotechnol (2006) 2.18
ProteomeBinders: planning a European resource of affinity reagents for analysis of the human proteome. Nat Methods (2007) 2.04
Designed to be stable: crystal structure of a consensus ankyrin repeat protein. Proc Natl Acad Sci U S A (2003) 1.98
Inhibition of caspase-2 by a designed ankyrin repeat protein: specificity, structure, and inhibition mechanism. Structure (2007) 1.93
Domain interactions in the Fab fragment: a comparative evaluation of the single-chain Fv and Fab format engineered with variable domains of different stability. J Mol Biol (2005) 1.90
A label-free immunosensor array using single-chain antibody fragments. Proc Natl Acad Sci U S A (2005) 1.85
Biophysical properties of human antibody variable domains. J Mol Biol (2003) 1.80
Allosteric inhibition of aminoglycoside phosphotransferase by a designed ankyrin repeat protein. Structure (2005) 1.75
A designed ankyrin repeat protein evolved to picomolar affinity to Her2. J Mol Biol (2007) 1.71
In-vitro protein evolution by ribosome display and mRNA display. J Immunol Methods (2004) 1.69
Ribosome display: selecting and evolving proteins in vitro that specifically bind to a target. Nat Methods (2007) 1.68
Folding and unfolding mechanism of highly stable full-consensus ankyrin repeat proteins. J Mol Biol (2007) 1.67
Design of multivalent complexes using the barnase*barstar module. Nat Biotechnol (2003) 1.65
Direct identification of ligand-receptor interactions on living cells and tissues. Nat Biotechnol (2012) 1.64
Directed evolution of a G protein-coupled receptor for expression, stability, and binding selectivity. Proc Natl Acad Sci U S A (2008) 1.63
Evolution of three human GPCRs for higher expression and stability. J Mol Biol (2011) 1.62
DARPins and other repeat protein scaffolds: advances in engineering and applications. Curr Opin Biotechnol (2011) 1.61
Efficient selection of DARPins with sub-nanomolar affinities using SRP phage display. J Mol Biol (2008) 1.57
Efficient tumor targeting with high-affinity designed ankyrin repeat proteins: effects of affinity and molecular size. Cancer Res (2010) 1.51
Directed in vitro evolution and crystallographic analysis of a peptide-binding single chain antibody fragment (scFv) with low picomolar affinity. J Biol Chem (2004) 1.46
Development of a generic adenovirus delivery system based on structure-guided design of bispecific trimeric DARPin adapters. Proc Natl Acad Sci U S A (2013) 1.46
Tunable signal processing in synthetic MAP kinase cascades. Cell (2011) 1.45
Designing repeat proteins: modular leucine-rich repeat protein libraries based on the mammalian ribonuclease inhibitor family. J Mol Biol (2003) 1.39
Stability improvement of antibodies for extracellular and intracellular applications: CDR grafting to stable frameworks and structure-based framework engineering. Methods (2004) 1.37
Designed armadillo repeat proteins as general peptide-binding scaffolds: consensus design and computational optimization of the hydrophobic core. J Mol Biol (2007) 1.33
Structure of a kinesin-tubulin complex and implications for kinesin motility. Nat Struct Mol Biol (2013) 1.31
PEGylation and multimerization of the anti-p185HER-2 single chain Fv fragment 4D5: effects on tumor targeting. J Biol Chem (2006) 1.26
Direct in vivo screening of intrabody libraries constructed on a highly stable single-chain framework. J Biol Chem (2002) 1.25
Engineered proteins as specific binding reagents. Curr Opin Biotechnol (2005) 1.24
Biophysical properties of camelid V(HH) domains compared to those of human V(H)3 domains. Biochemistry (2002) 1.22
Stepwise unfolding of ankyrin repeats in a single protein revealed by atomic force microscopy. Biophys J (2005) 1.20
Folding of a designed simple ankyrin repeat protein. Protein Sci (2004) 1.20
Crystal structure and function of a DARPin neutralizing inhibitor of lactococcal phage TP901-1: comparison of DARPin and camelid VHH binding mode. J Biol Chem (2009) 1.19
Intracellular kinase inhibitors selected from combinatorial libraries of designed ankyrin repeat proteins. J Biol Chem (2005) 1.17
A designed ankyrin repeat protein selected to bind to tubulin caps the microtubule plus end. Proc Natl Acad Sci U S A (2012) 1.16
A novel strategy to design binding molecules harnessing the modular nature of repeat proteins. FEBS Lett (2003) 1.15
Selection and characterization of Her2 binding-designed ankyrin repeat proteins. J Biol Chem (2006) 1.15
Structure of signaling-competent neurotensin receptor 1 obtained by directed evolution in Escherichia coli. Proc Natl Acad Sci U S A (2014) 1.15
Protein PEGylation decreases observed target association rates via a dual blocking mechanism. Mol Pharmacol (2005) 1.14
A recombinant immunotoxin derived from a humanized epithelial cell adhesion molecule-specific single-chain antibody fragment has potent and selective antitumor activity. Clin Cancer Res (2003) 1.13
Robust network topologies for generating switch-like cellular responses. PLoS Comput Biol (2011) 1.12
Critical features for biosynthesis, stability, and functionality of a G protein-coupled receptor uncovered by all-versus-all mutations. Proc Natl Acad Sci U S A (2012) 1.09
Affinity-matured recombinant antibody fragments analyzed by single-molecule force spectroscopy. Biophys J (2007) 1.09
DARPins: an efficient targeting domain for lentiviral vectors. Mol Ther (2011) 1.08
Turnover-based in vitro selection and evolution of biocatalysts from a fully synthetic antibody library. Nat Biotechnol (2003) 1.06
Structure-based improvement of the biophysical properties of immunoglobulin VH domains with a generalizable approach. Biochemistry (2003) 1.06
Designed ankyrin repeat proteins (DARPins) from research to therapy. Methods Enzymol (2012) 1.06
Directed evolution of an anti-prion protein scFv fragment to an affinity of 1 pM and its structural interpretation. J Mol Biol (2006) 1.06
In vitro selection and characterization of DARPins and Fab fragments for the co-crystallization of membrane proteins: The Na(+)-citrate symporter CitS as an example. J Struct Biol (2007) 1.03
Structural determinants for improved stability of designed ankyrin repeat proteins with a redesigned C-capping module. J Mol Biol (2010) 1.02
Structural and functional analysis of phosphorylation-specific binders of the kinase ERK from designed ankyrin repeat protein libraries. Proc Natl Acad Sci U S A (2012) 1.01
An antibody library for stabilizing and crystallizing membrane proteins - selecting binders to the citrate carrier CitS. FEBS Lett (2004) 1.00
Transfer of engineered biophysical properties between different antibody formats and expression systems. Protein Eng Des Sel (2012) 1.00
Bispecific designed ankyrin repeat proteins (DARPins) targeting epidermal growth factor receptor inhibit A431 cell proliferation and receptor recycling. J Biol Chem (2011) 1.00
Stabilizing ionic interactions in a full-consensus ankyrin repeat protein. J Mol Biol (2007) 0.99
EpCAM-targeted delivery of nanocomplexed siRNA to tumor cells with designed ankyrin repeat proteins. Mol Cancer Ther (2009) 0.99
Consensus design of repeat proteins. Chembiochem (2004) 0.98
Structural basis for eliciting a cytotoxic effect in HER2-overexpressing cancer cells via binding to the extracellular domain of HER2. Structure (2013) 0.98
Antitumor activity of an epithelial cell adhesion molecule targeted nanovesicular drug delivery system. Mol Cancer Ther (2007) 0.97
Effective phagocytosis of low Her2 tumor cell lines with engineered, aglycosylated IgG displaying high FcγRIIa affinity and selectivity. ACS Chem Biol (2012) 0.97
Positive receptor feedback during lineage commitment can generate ultrasensitivity to ligand and confer robustness to a bistable switch. Biophys J (2008) 0.97
Protein tag-mediated conjugation of oligonucleotides to recombinant affinity binders for proximity ligation. N Biotechnol (2012) 0.97
DARPins recognizing the tumor-associated antigen EpCAM selected by phage and ribosome display and engineered for multivalency. J Mol Biol (2011) 0.97
Direct molecular evolution of detergent-stable G protein-coupled receptors using polymer encapsulated cells. J Mol Biol (2012) 0.97
Stabilizing membrane proteins through protein engineering. Curr Opin Chem Biol (2013) 0.96
Maximizing detergent stability and functional expression of a GPCR by exhaustive recombination and evolution. J Mol Biol (2012) 0.96
Isolation of intracellular proteinase inhibitors derived from designed ankyrin repeat proteins by genetic screening. J Biol Chem (2006) 0.95
Comparison of in vivo selection and rational design of heterodimeric coiled coils. Structure (2002) 0.95
Crystal structure of a consensus-designed ankyrin repeat protein: implications for stability. Proteins (2006) 0.94
Epithelial cell adhesion molecule-targeted drug delivery for cancer therapy. Expert Opin Drug Deliv (2013) 0.94
Characterization and further stabilization of designed ankyrin repeat proteins by combining molecular dynamics simulations and experiments. J Mol Biol (2007) 0.94
Knowledge-based design of a biosensor to quantify localized ERK activation in living cells. Chem Biol (2013) 0.93
Direct selection of antibodies from complex libraries with the protein fragment complementation assay. J Mol Biol (2006) 0.93
Hematopoiesis and its disorders: a systems biology approach. Blood (2010) 0.93
Designed ankyrin repeat proteins (DARPins) as novel isoform-specific intracellular inhibitors of c-Jun N-terminal kinases. ACS Chem Biol (2012) 0.93
The influence of the framework core residues on the biophysical properties of immunoglobulin heavy chain variable domains. Protein Eng Des Sel (2009) 0.93
Synthetic conversion of a graded receptor signal into a tunable, reversible switch. Mol Syst Biol (2011) 0.92
Selection and characterization of DARPins specific for the neurotensin receptor 1. Protein Eng Des Sel (2009) 0.92
Crystal structure of the anti-His tag antibody 3D5 single-chain fragment complexed to its antigen. J Mol Biol (2002) 0.92
Ribosome display: a technology for selecting and evolving proteins from large libraries. Methods Mol Biol (2011) 0.92
Stabilization and humanization of a single-chain Fv antibody fragment specific for human lymphocyte antigen CD19 by designed point mutations and CDR-grafting onto a human framework. Protein Eng Des Sel (2009) 0.91
Selection based on the folding properties of proteins with ribosome display. FEBS Lett (2003) 0.91
Construction and characterization of protein libraries composed of secondary structure modules. Protein Sci (2002) 0.91
Engineering aggregation resistance in IgG by two independent mechanisms: lessons from comparison of Pichia pastoris and mammalian cell expression. J Mol Biol (2012) 0.89
Residue-resolved stability of full-consensus ankyrin repeat proteins probed by NMR. J Mol Biol (2010) 0.89
Integrating extrinsic and intrinsic cues into a minimal model of lineage commitment for hematopoietic progenitors. PLoS Comput Biol (2009) 0.89
Facile double-functionalization of designed ankyrin repeat proteins using click and thiol chemistries. Bioconjug Chem (2012) 0.89
Her2-specific multivalent adapters confer designed tropism to adenovirus for gene targeting. J Mol Biol (2010) 0.89
Rapid selection of high-affinity binders using ribosome display. Methods Mol Biol (2012) 0.89
Nisin-inducible secretion of a biologically active single-chain insulin analog by Lactococcus lactis NZ9000. Biotechnol Bioeng (2011) 0.89
Rapid selection of specific MAP kinase-binders from designed ankyrin repeat protein libraries. Protein Eng Des Sel (2006) 0.89
DARPin-targeting of measles virus: unique bispecificity, effective oncolysis, and enhanced safety. Mol Ther (2013) 0.88
In vitro selection for catalytic activity with ribosome display. J Am Chem Soc (2002) 0.86
NMR solution structure of the monomeric form of the bacteriophage lambda capsid stabilizing protein gpD. J Biomol NMR (2005) 0.86
Engineering signal peptides for enhanced protein secretion from Lactococcus lactis. Appl Environ Microbiol (2012) 0.86