Published in J Mol Biol on July 21, 2010
Development of a generic adenovirus delivery system based on structure-guided design of bispecific trimeric DARPin adapters. Proc Natl Acad Sci U S A (2013) 1.46
Nucleic acid recognition by tandem helical repeats. Curr Opin Struct Biol (2011) 1.06
Highly potent VEGF-A-antagonistic DARPins as anti-angiogenic agents for topical and intravitreal applications. Angiogenesis (2012) 0.86
Modulation of the multistate folding of designed TPR proteins through intrinsic and extrinsic factors. Protein Sci (2012) 0.86
Optimization of designed armadillo repeat proteins by molecular dynamics simulations and NMR spectroscopy. Protein Sci (2012) 0.84
Long-range effects and functional consequences of stabilizing mutations in the ankyrin repeat domain of IκBα. J Mol Biol (2012) 0.83
Peptide aptamers: development and applications. Curr Top Med Chem (2015) 0.80
Crystal structures of designed armadillo repeat proteins: implications of construct design and crystallization conditions on overall structure. Protein Sci (2014) 0.80
StaRProtein, a web server for prediction of the stability of repeat proteins. PLoS One (2015) 0.75
Engineering novel binding proteins from nonimmunoglobulin domains. Nat Biotechnol (2005) 3.67
High-affinity binders selected from designed ankyrin repeat protein libraries. Nat Biotechnol (2004) 3.34
Designing repeat proteins: well-expressed, soluble and stable proteins from combinatorial libraries of consensus ankyrin repeat proteins. J Mol Biol (2003) 2.81
Signal sequences directing cotranslational translocation expand the range of proteins amenable to phage display. Nat Biotechnol (2006) 2.18
Wordom: a program for efficient analysis of molecular dynamics simulations. Bioinformatics (2007) 2.05
ProteomeBinders: planning a European resource of affinity reagents for analysis of the human proteome. Nat Methods (2007) 2.04
Designed to be stable: crystal structure of a consensus ankyrin repeat protein. Proc Natl Acad Sci U S A (2003) 1.98
Inhibition of caspase-2 by a designed ankyrin repeat protein: specificity, structure, and inhibition mechanism. Structure (2007) 1.93
Domain interactions in the Fab fragment: a comparative evaluation of the single-chain Fv and Fab format engineered with variable domains of different stability. J Mol Biol (2005) 1.90
A label-free immunosensor array using single-chain antibody fragments. Proc Natl Acad Sci U S A (2005) 1.85
Biophysical properties of human antibody variable domains. J Mol Biol (2003) 1.80
Allosteric inhibition of aminoglycoside phosphotransferase by a designed ankyrin repeat protein. Structure (2005) 1.75
A designed ankyrin repeat protein evolved to picomolar affinity to Her2. J Mol Biol (2007) 1.71
In-vitro protein evolution by ribosome display and mRNA display. J Immunol Methods (2004) 1.69
Ribosome display: selecting and evolving proteins in vitro that specifically bind to a target. Nat Methods (2007) 1.68
Folding and unfolding mechanism of highly stable full-consensus ankyrin repeat proteins. J Mol Biol (2007) 1.67
Design of multivalent complexes using the barnase*barstar module. Nat Biotechnol (2003) 1.65
Direct identification of ligand-receptor interactions on living cells and tissues. Nat Biotechnol (2012) 1.64
Directed evolution of a G protein-coupled receptor for expression, stability, and binding selectivity. Proc Natl Acad Sci U S A (2008) 1.63
Evolution of three human GPCRs for higher expression and stability. J Mol Biol (2011) 1.62
DARPins and other repeat protein scaffolds: advances in engineering and applications. Curr Opin Biotechnol (2011) 1.61
Efficient selection of DARPins with sub-nanomolar affinities using SRP phage display. J Mol Biol (2008) 1.57
Efficient tumor targeting with high-affinity designed ankyrin repeat proteins: effects of affinity and molecular size. Cancer Res (2010) 1.51
Directed in vitro evolution and crystallographic analysis of a peptide-binding single chain antibody fragment (scFv) with low picomolar affinity. J Biol Chem (2004) 1.46
Development of a generic adenovirus delivery system based on structure-guided design of bispecific trimeric DARPin adapters. Proc Natl Acad Sci U S A (2013) 1.46
Designing repeat proteins: modular leucine-rich repeat protein libraries based on the mammalian ribonuclease inhibitor family. J Mol Biol (2003) 1.39
Stability improvement of antibodies for extracellular and intracellular applications: CDR grafting to stable frameworks and structure-based framework engineering. Methods (2004) 1.37
Designed armadillo repeat proteins as general peptide-binding scaffolds: consensus design and computational optimization of the hydrophobic core. J Mol Biol (2007) 1.33
Structure of a kinesin-tubulin complex and implications for kinesin motility. Nat Struct Mol Biol (2013) 1.31
Toward the rational design of p53-stabilizing drugs: probing the surface of the oncogenic Y220C mutant. Chem Biol (2010) 1.27
PEGylation and multimerization of the anti-p185HER-2 single chain Fv fragment 4D5: effects on tumor targeting. J Biol Chem (2006) 1.26
Direct in vivo screening of intrabody libraries constructed on a highly stable single-chain framework. J Biol Chem (2002) 1.25
Engineered proteins as specific binding reagents. Curr Opin Biotechnol (2005) 1.24
Biophysical properties of camelid V(HH) domains compared to those of human V(H)3 domains. Biochemistry (2002) 1.22
Folding of a designed simple ankyrin repeat protein. Protein Sci (2004) 1.20
Stepwise unfolding of ankyrin repeats in a single protein revealed by atomic force microscopy. Biophys J (2005) 1.20
Crystal structure and function of a DARPin neutralizing inhibitor of lactococcal phage TP901-1: comparison of DARPin and camelid VHH binding mode. J Biol Chem (2009) 1.19
Prolyl isomerase PIN1 regulates DNA double-strand break repair by counteracting DNA end resection. Mol Cell (2013) 1.19
Intracellular kinase inhibitors selected from combinatorial libraries of designed ankyrin repeat proteins. J Biol Chem (2005) 1.17
A designed ankyrin repeat protein selected to bind to tubulin caps the microtubule plus end. Proc Natl Acad Sci U S A (2012) 1.16
Structural and biochemical studies on procaspase-8: new insights on initiator caspase activation. Structure (2009) 1.16
Using a beta-hairpin to mimic an alpha-helix: cyclic peptidomimetic inhibitors of the p53-HDM2 protein-protein interaction. Angew Chem Int Ed Engl (2004) 1.16
Selection and characterization of Her2 binding-designed ankyrin repeat proteins. J Biol Chem (2006) 1.15
A novel strategy to design binding molecules harnessing the modular nature of repeat proteins. FEBS Lett (2003) 1.15
Structure of signaling-competent neurotensin receptor 1 obtained by directed evolution in Escherichia coli. Proc Natl Acad Sci U S A (2014) 1.15
Protein PEGylation decreases observed target association rates via a dual blocking mechanism. Mol Pharmacol (2005) 1.14
Multiple conformations of full-length p53 detected with single-molecule fluorescence resonance energy transfer. Proc Natl Acad Sci U S A (2009) 1.13
A recombinant immunotoxin derived from a humanized epithelial cell adhesion molecule-specific single-chain antibody fragment has potent and selective antitumor activity. Clin Cancer Res (2003) 1.13
Critical features for biosynthesis, stability, and functionality of a G protein-coupled receptor uncovered by all-versus-all mutations. Proc Natl Acad Sci U S A (2012) 1.09
Affinity-matured recombinant antibody fragments analyzed by single-molecule force spectroscopy. Biophys J (2007) 1.09
DARPins: an efficient targeting domain for lentiviral vectors. Mol Ther (2011) 1.08
The intracellular antibody capture technology (IACT): towards a consensus sequence for intracellular antibodies. J Mol Biol (2002) 1.08
Turnover-based in vitro selection and evolution of biocatalysts from a fully synthetic antibody library. Nat Biotechnol (2003) 1.06
Structure-based improvement of the biophysical properties of immunoglobulin VH domains with a generalizable approach. Biochemistry (2003) 1.06
Directed evolution of an anti-prion protein scFv fragment to an affinity of 1 pM and its structural interpretation. J Mol Biol (2006) 1.06
Designed ankyrin repeat proteins (DARPins) from research to therapy. Methods Enzymol (2012) 1.06
Structure-activity studies in a family of beta-hairpin protein epitope mimetic inhibitors of the p53-HDM2 protein-protein interaction. Chembiochem (2006) 1.04
In vitro selection and characterization of DARPins and Fab fragments for the co-crystallization of membrane proteins: The Na(+)-citrate symporter CitS as an example. J Struct Biol (2007) 1.03
Structural determinants for improved stability of designed ankyrin repeat proteins with a redesigned C-capping module. J Mol Biol (2010) 1.02
Estimation of protein folding probability from equilibrium simulations. J Chem Phys (2005) 1.02
Structural and functional analysis of phosphorylation-specific binders of the kinase ERK from designed ankyrin repeat protein libraries. Proc Natl Acad Sci U S A (2012) 1.01
Transfer of engineered biophysical properties between different antibody formats and expression systems. Protein Eng Des Sel (2012) 1.00
An antibody library for stabilizing and crystallizing membrane proteins - selecting binders to the citrate carrier CitS. FEBS Lett (2004) 1.00
Bispecific designed ankyrin repeat proteins (DARPins) targeting epidermal growth factor receptor inhibit A431 cell proliferation and receptor recycling. J Biol Chem (2011) 1.00
Stabilizing ionic interactions in a full-consensus ankyrin repeat protein. J Mol Biol (2007) 0.99
Mechanical unfolding of an ankyrin repeat protein. Biophys J (2010) 0.99
EpCAM-targeted delivery of nanocomplexed siRNA to tumor cells with designed ankyrin repeat proteins. Mol Cancer Ther (2009) 0.99
Surprisingly stable helical conformations in alpha/beta-peptides by incorporation of cis-beta-aminocyclopropane carboxylic acids. Angew Chem Int Ed Engl (2004) 0.99
Consensus design of repeat proteins. Chembiochem (2004) 0.98
Structural basis for eliciting a cytotoxic effect in HER2-overexpressing cancer cells via binding to the extracellular domain of HER2. Structure (2013) 0.98
Antitumor activity of an epithelial cell adhesion molecule targeted nanovesicular drug delivery system. Mol Cancer Ther (2007) 0.97
Protein tag-mediated conjugation of oligonucleotides to recombinant affinity binders for proximity ligation. N Biotechnol (2012) 0.97
DARPins recognizing the tumor-associated antigen EpCAM selected by phage and ribosome display and engineered for multivalency. J Mol Biol (2011) 0.97
Interactions of lipopolysaccharide and polymyxin studied by NMR spectroscopy. J Biol Chem (2009) 0.97
Direct molecular evolution of detergent-stable G protein-coupled receptors using polymer encapsulated cells. J Mol Biol (2012) 0.97
Stabilizing membrane proteins through protein engineering. Curr Opin Chem Biol (2013) 0.96
Maximizing detergent stability and functional expression of a GPCR by exhaustive recombination and evolution. J Mol Biol (2012) 0.96
Comparison of in vivo selection and rational design of heterodimeric coiled coils. Structure (2002) 0.95
Isolation of intracellular proteinase inhibitors derived from designed ankyrin repeat proteins by genetic screening. J Biol Chem (2006) 0.95
Crystal structure of a consensus-designed ankyrin repeat protein: implications for stability. Proteins (2006) 0.94
Epithelial cell adhesion molecule-targeted drug delivery for cancer therapy. Expert Opin Drug Deliv (2013) 0.94
Characterization and further stabilization of designed ankyrin repeat proteins by combining molecular dynamics simulations and experiments. J Mol Biol (2007) 0.94
Knowledge-based design of a biosensor to quantify localized ERK activation in living cells. Chem Biol (2013) 0.93
Computational analysis of off-rate selection experiments to optimize affinity maturation by directed evolution. Protein Eng Des Sel (2010) 0.93
Direct selection of antibodies from complex libraries with the protein fragment complementation assay. J Mol Biol (2006) 0.93
Designed ankyrin repeat proteins (DARPins) as novel isoform-specific intracellular inhibitors of c-Jun N-terminal kinases. ACS Chem Biol (2012) 0.93
The influence of the framework core residues on the biophysical properties of immunoglobulin heavy chain variable domains. Protein Eng Des Sel (2009) 0.93
Structural similarities of micelle-bound peptide YY (PYY) and neuropeptide Y (NPY) are related to their affinity profiles at the Y receptors. J Mol Biol (2004) 0.93
Ribosome display: a technology for selecting and evolving proteins from large libraries. Methods Mol Biol (2011) 0.92
Crystal structure of the anti-His tag antibody 3D5 single-chain fragment complexed to its antigen. J Mol Biol (2002) 0.92
Selection and characterization of DARPins specific for the neurotensin receptor 1. Protein Eng Des Sel (2009) 0.92
Selection based on the folding properties of proteins with ribosome display. FEBS Lett (2003) 0.91
Construction and characterization of protein libraries composed of secondary structure modules. Protein Sci (2002) 0.91
Stabilization and humanization of a single-chain Fv antibody fragment specific for human lymphocyte antigen CD19 by designed point mutations and CDR-grafting onto a human framework. Protein Eng Des Sel (2009) 0.91
New mosquito larvicidal tetranortriterpenoids from Turraea wakefieldii and Turraea floribunda. J Agric Food Chem (2004) 0.90
Identification of a basic surface area of the FADD death effector domain critical for apoptotic signaling. FEBS Lett (2002) 0.89
Kinetic response of a photoperturbed allosteric protein. Proc Natl Acad Sci U S A (2013) 0.89