Observing protein interactions and their stoichiometry in living cells by brightness analysis of fluorescence fluctuation experiments.

PubWeight™: 0.95‹?› | Rank: Top 15%

🔗 View Article (PMID 20580971)

Published in Methods Enzymol on January 01, 2010

Authors

Yan Chen1, Jolene Johnson, Patrick Macdonald, Bin Wu, Joachim D Mueller

Author Affiliations

1: School of Physics and Astronomy, University of Minnesota, Minneapolis, Minnesota, USA.

Articles citing this

The impact of heterogeneity and dark acceptor states on FRET: implications for using fluorescent protein donors and acceptors. PLoS One (2012) 0.97

Chromophore maturation and fluorescence fluctuation spectroscopy of fluorescent proteins in a cell-free expression system. Anal Biochem (2011) 0.95

Fluorescence polarization and fluctuation analysis monitors subunit proximity, stoichiometry, and protein complex hydrodynamics. PLoS One (2012) 0.91

Quantifying protein-protein interactions of peripheral membrane proteins by fluorescence brightness analysis. Biophys J (2014) 0.91

APOBEC3 multimerization correlates with HIV-1 packaging and restriction activity in living cells. J Mol Biol (2013) 0.90

Quantifying the dynamics of the oligomeric transcription factor STAT3 by pair correlation of molecular brightness. Nat Commun (2016) 0.85

New insights into HTLV-1 particle structure, assembly, and Gag-Gag interactions in living cells. Viruses (2011) 0.82

Molecular brightness analysis reveals phosphatidylinositol 4-Kinase IIβ association with clathrin-coated vesicles in living cells. Biophys J (2012) 0.81

Coexistence of phases in a protein heterodimer. J Chem Phys (2012) 0.78

Automated Analysis of Single-Molecule Photobleaching Data by Statistical Modeling of Spot Populations. Biophys J (2015) 0.78

Quantifying intracellular dynamics using fluorescence fluctuation spectroscopy. Protoplasma (2014) 0.76

Association of Endophilin B1 with Cytoplasmic Vesicles. Biophys J (2016) 0.75

Deciphering CaMKII Multimerization Using Fluorescence Correlation Spectroscopy and Homo-FRET Analysis. Biophys J (2017) 0.75

Perturbation of Human T-Cell Leukemia Virus Type 1 Particle Morphology by Differential Gag Co-Packaging. Viruses (2017) 0.75

Articles by these authors

Real-time observation of transcription initiation and elongation on an endogenous yeast gene. Science (2011) 3.67

(E)-1-{4-[Bis(4-meth-oxy-phen-yl)meth-yl]piperazin-1-yl}-3-(4-fluoro-phen-yl)prop-2-en-1-one. Acta Crystallogr Sect E Struct Rep Online (2011) 3.63

High expression levels of putative hepatic stem/progenitor cell biomarkers related to tumour angiogenesis and poor prognosis of hepatocellular carcinoma. Gut (2010) 2.58

Single β-actin mRNA detection in neurons reveals a mechanism for regulating its translatability. Science (2014) 2.23

Preferences for self-management support: findings from a survey of diabetes patients in safety-net health systems. Patient Educ Couns (2007) 2.04

Cooperative assembly and dynamic disassembly of MDA5 filaments for viral dsRNA recognition. Proc Natl Acad Sci U S A (2011) 1.99

An NMR approach to structural proteomics. Proc Natl Acad Sci U S A (2002) 1.95

Association of autophagy defect with a malignant phenotype and poor prognosis of hepatocellular carcinoma. Cancer Res (2008) 1.85

Response of soil respiration to soil temperature and moisture in a 50-year-old oriental arborvitae plantation in China. PLoS One (2011) 1.80

Blood pressure and stroke risk among diabetic patients. J Clin Endocrinol Metab (2013) 1.79

A novel loss-of-function DDAH1 promoter polymorphism is associated with increased susceptibility to thrombosis stroke and coronary heart disease. Circ Res (2010) 1.70

Evaluation of the biocompatibility of a chitosan scaffold in mice. J Biomed Mater Res (2002) 1.62

RIG-I forms signaling-competent filaments in an ATP-dependent, ubiquitin-independent manner. Mol Cell (2013) 1.57

Retroperitoneal laparoscopic anatrophic nephrolithotomy for large staghorn calculi. Int J Urol (2010) 1.53

Xom interacts with and stimulates transcriptional activity of LEF1/TCFs: implications for ventral cell fate determination during vertebrate embryogenesis. Cell Res (2007) 1.51

Rosuvastatin attenuates the elevation in blood pressure induced by overexpression of human C-reactive protein. Hypertens Res (2011) 1.50

Uniform hexagonal graphene flakes and films grown on liquid copper surface. Proc Natl Acad Sci U S A (2012) 1.49

Monomeric red fluorescent proteins with a large Stokes shift. Proc Natl Acad Sci U S A (2010) 1.47

(E)-1-(4-Benzhydrylpiperazin-1-yl)-3-(3,4-dieth-oxy-phen-yl)prop-2-en-1-one ethanol monosolvate. Acta Crystallogr Sect E Struct Rep Online (2011) 1.46

PUMA-mediated intestinal epithelial apoptosis contributes to ulcerative colitis in humans and mice. J Clin Invest (2011) 1.43

PUMA sensitizes lung cancer cells to chemotherapeutic agents and irradiation. Clin Cancer Res (2006) 1.43

Second- versus first-generation azoles for antifungal prophylaxis in hematology patients: a systematic review and meta-analysis. Ann Hematol (2013) 1.39

Retracted Reanalysis of the gene expression profile in chronic pancreatitis via bioinformatics methods. Eur J Med Res (2014) 1.39

(E)-1-{4-[Bis(4-meth-oxy-phen-yl)meth-yl]piperazin-1-yl}-3-(3,4-dieth-oxy-phen-yl)prop-2-en-1-one ethanol disolvate. Acta Crystallogr Sect E Struct Rep Online (2012) 1.39

Studies related to the relative thermodynamic stability of C-terminal peptidyl esters of O-hydroxy thiophenol: emergence of a doable strategy for non-cysteine ligation applicable to the chemical synthesis of glycopeptides. J Am Chem Soc (2006) 1.37

Visual disability, visual function, and myopia among rural chinese secondary school children: the Xichang Pediatric Refractive Error Study (X-PRES)--report 1. Invest Ophthalmol Vis Sci (2008) 1.34

Kinetic mechanism for viral dsRNA length discrimination by MDA5 filaments. Proc Natl Acad Sci U S A (2012) 1.33

A universal method for the preparation of covalent protein-DNA conjugates for use in creating protein nanostructures. Angew Chem Int Ed Engl (2007) 1.33

Molecular basis of Pirh2-mediated p53 ubiquitylation. Nat Struct Mol Biol (2008) 1.32

Increasing prevalence of diabetes in middle or low income residents in Louisiana from 2000 to 2009. Diabetes Res Clin Pract (2011) 1.32

Dual-color photon-counting histogram. Biophys J (2004) 1.29

Genome-wide identification and characterization of novel genes involved in terpenoid biosynthesis in Salvia miltiorrhiza. J Exp Bot (2012) 1.26

Total vs. proximal gastrectomy for proximal gastric cancer: a systematic review and meta-analysis. Hepatogastroenterology (2012) 1.24

The mechanics behind DNA sequence-dependent properties of the nucleosome. Nucleic Acids Res (2012) 1.23

Blind testing of routine, fully automated determination of protein structures from NMR data. Structure (2012) 1.23

CD24 is a novel predictor for poor prognosis of hepatocellular carcinoma after surgery. Clin Cancer Res (2009) 1.22

Isolation, antimicrobial resistance, and virulence genes of Pasteurella multocida strains from swine in China. J Clin Microbiol (2009) 1.22

Racial disparities in diabetic complications in an underinsured population. J Clin Endocrinol Metab (2012) 1.22

Aggressive blood pressure control increases coronary heart disease risk among diabetic patients. Diabetes Care (2013) 1.21

Toward homogeneous erythropoietin: chemical synthesis of the Ala1-Gly28 glycopeptide domain by "alanine" ligation. J Am Chem Soc (2009) 1.21

MicroRNA-195-5p suppresses glucose uptake and proliferation of human bladder cancer T24 cells by regulating GLUT3 expression. FEBS Lett (2012) 1.20

(E)-1-{4-[Bis(4-bromo-phen-yl)meth-yl]piperazin-1-yl}-3-(4-eth-oxy-3-meth-oxy-phen-yl)prop-2-en-1-one. Acta Crystallogr Sect E Struct Rep Online (2011) 1.19

Persistence of side population cells with high drug efflux capacity in pancreatic cancer. World J Gastroenterol (2008) 1.18

(E)-1-{4-[Bis(4-meth-oxy-phen-yl)meth-yl]piperazin-1-yl}-3-(4-methyl-phen-yl)prop-2-en-1-one. Acta Crystallogr Sect E Struct Rep Online (2011) 1.17

Partial deletions are associated with an increased risk of complete deletion in AZFc: a new insight into the role of partial AZFc deletions in male infertility. J Med Genet (2007) 1.16

Characteristics of gastroesophageal reflux disease in Japan: increased prevalence in elderly women. J Gastroenterol (2003) 1.15

The pathogenesis of severe fever with thrombocytopenia syndrome virus infection in alpha/beta interferon knockout mice: insights into the pathologic mechanisms of a new viral hemorrhagic fever. J Virol (2013) 1.13

The b2/b3 subdeletion shows higher risk of spermatogenic failure and higher frequency of complete AZFc deletion than the gr/gr subdeletion in a Chinese population. Hum Mol Genet (2008) 1.13

A generalized description of the elastic properties of nanowires. Nano Lett (2006) 1.13

Cytokine and chemokine levels in patients infected with the novel avian influenza A (H7N9) virus in China. J Infect Dis (2013) 1.13

β-Actin mRNA compartmentalization enhances focal adhesion stability and directs cell migration. Genes Dev (2012) 1.13

Increased expression of MMP9 is correlated with poor prognosis of nasopharyngeal carcinoma. BMC Cancer (2010) 1.11

Increasing incidence of gestational diabetes mellitus in Louisiana, 1997-2009. J Womens Health (Larchmt) (2011) 1.11

(E)-1-{4-[Bis(4-fluoro-phen-yl)meth-yl]piperazin-1-yl}-3-(4-eth-oxy-phen-yl)prop-2-en-1-one. Acta Crystallogr Sect E Struct Rep Online (2011) 1.11

miR-21 downregulated TCF21 to inhibit KISS1 in renal cancer. Urology (2012) 1.10

DNA stretching in the nucleosome facilitates alkylation by an intercalating antitumour agent. Nucleic Acids Res (2009) 1.10

Mutation in the SYNJ1 gene associated with autosomal recessive, early-onset Parkinsonism. Hum Mutat (2013) 1.10

Fluorescence fluctuation spectroscopy on viral-like particles reveals variable gag stoichiometry. Biophys J (2009) 1.10

Hollow ARROW Waveguides on Self-Aligned Pedestals for Improved Geometry and Transmission. IEEE Photonics Technol Lett (2010) 1.09

Detection of severe fever with thrombocytopenia syndrome virus by reverse transcription-cross-priming amplification coupled with vertical flow visualization. J Clin Microbiol (2012) 1.09

Rapid and sensitive detection of novel avian-origin influenza A (H7N9) virus by reverse transcription loop-mediated isothermal amplification combined with a lateral-flow device. PLoS One (2013) 1.09

Brightness analysis by Z-scan fluorescence fluctuation spectroscopy for the study of protein interactions within living cells. Biophys J (2010) 1.09

Oxygen-aided synthesis of polycrystalline graphene on silicon dioxide substrates. J Am Chem Soc (2011) 1.08

Hispolon induces apoptosis in human gastric cancer cells through a ROS-mediated mitochondrial pathway. Free Radic Biol Med (2008) 1.08

(E)-1-{4-[Bis(4-bromo-phen-yl)meth-yl]piperazin-1-yl}-3-(4-bromo-phen-yl)prop-2-en-1-one. Acta Crystallogr Sect E Struct Rep Online (2011) 1.07

Racial differences in the association between gestational diabetes mellitus and risk of type 2 diabetes. J Womens Health (Larchmt) (2012) 1.06

Icariin and its derivative icariside II extend healthspan via insulin/IGF-1 pathway in C. elegans. PLoS One (2011) 1.06

Molecular characterization of transcriptome-wide interactions between highly pathogenic porcine reproductive and respiratory syndrome virus and porcine alveolar macrophages in vivo. Int J Biol Sci (2011) 1.06

Comparative transcriptional profiling and preliminary study on heterosis mechanism of super-hybrid rice. Mol Plant (2010) 1.05

Adeno-associated virus-mediated osteoprotegerin gene transfer protects against particulate polyethylene-induced osteolysis in a murine model. Arthritis Rheum (2002) 1.05

Regulation of local expression of cell adhesion and motility-related mRNAs in breast cancer cells by IMP1/ZBP1. J Cell Sci (2012) 1.05

HbA1c and coronary heart disease risk among diabetic patients. Diabetes Care (2013) 1.05

Increasing prevalence of hypertension in low income residents within Louisiana State University Health Care Services Division Hospital System. Eur J Intern Med (2012) 1.05

Dapagliflozin treatment for type 2 diabetes: a systematic review and meta-analysis of randomized controlled trials. Diabetes Metab Res Rev (2014) 1.04

Barriers to physical activity among predominantly low-income African-American patients with type 2 diabetes. Diabetes Care (2005) 1.04

Muscle histopathology in nebulin-related nemaline myopathy: ultrastrastructural findings correlated to disease severity and genotype. Acta Neuropathol Commun (2014) 1.04

Association of common variants of CYP4A11 and CYP4F2 with stroke in the Han Chinese population. Pharmacogenet Genomics (2010) 1.04

Mature Homogeneous Erythropoietin-Level Building Blocks by Chemical Synthesis: The EPO 114-166 Glycopeptide Domain, Presenting the O-Linked Glycophorin. Tetrahedron Lett (2006) 1.03

1-[Bis(4-fluoro-phen-yl)meth-yl]-4-[2-(2-methyl-phen-oxy)eth-yl]piperazine. Acta Crystallogr Sect E Struct Rep Online (2012) 1.03

Association between UHMWPE particle-induced inflammatory osteoclastogenesis and expression of RANKL, VEGF, and Flt-1 in vivo. Biomaterials (2006) 1.02

Production of high-quality carbon nanoscrolls with microwave spark assistance in liquid nitrogen. Adv Mater (2011) 1.02

Seroepidemiology of human enterovirus71 and coxsackievirusA16 in Jiangsu province, China. Virol J (2012) 1.02

Macrophage depletion diminishes implant-wear-induced inflammatory osteolysis in a mouse model. J Biomed Mater Res A (2008) 1.01

Synthesis and evaluation of the cytotoxicity of apoptolidinones A and D. J Org Chem (2008) 1.01

Berberine inhibits metastasis of nasopharyngeal carcinoma 5-8F cells by targeting Rho kinase-mediated Ezrin phosphorylation at threonine 567. J Biol Chem (2009) 1.01

Implant wear induces inflammation, but not osteoclastic bone resorption, in RANK(-/-) mice. J Orthop Res (2006) 1.01

Elevated body burdens of PBDEs, dioxins, and PCBs on thyroid hormone homeostasis at an electronic waste recycling site in China. Environ Sci Technol (2010) 1.01

Reassortant H9N2 influenza viruses containing H5N1-like PB1 genes isolated from black-billed magpies in Southern China. PLoS One (2011) 1.01

Improvement of image quality and radiation dose of CT perfusion of the brain by means of low-tube voltage (70 KV). Eur Radiol (2014) 1.00

Elevated expression of CDK4 in lung cancer. J Transl Med (2011) 1.00

Biophysical analysis of HTLV-1 particles reveals novel insights into particle morphology and Gag stochiometry. Retrovirology (2010) 1.00

Severe hypersensitivity dermatitis and liver dysfunction induced by occupational exposure to trichloroethylene. Ind Health (2009) 0.99

β-Elemene induces apoptosis in human renal-cell carcinoma 786-0 cells through inhibition of MAPK/ERK and PI3K/Akt/ mTOR signalling pathways. Asian Pac J Cancer Prev (2012) 0.98

Evolution of cooperation driven by reputation-based migration. PLoS One (2012) 0.98

Event (error and near-miss) reporting and learning system for process improvement in radiation oncology. Med Phys (2010) 0.98

Murine model of prosthesis failure for the long-term study of aseptic loosening. J Orthop Res (2007) 0.97

The occurrence of Bordetella bronchiseptica in pigs with clinical respiratory disease. Vet J (2010) 0.97

Association of distinctive Epstein-Barr virus variants with gastric carcinoma in Guangzhou, southern China. J Med Virol (2010) 0.96

Atomic details of near-transition state conformers for enzyme phosphoryl transfer revealed by MgF-3 rather than by phosphoranes. Proc Natl Acad Sci U S A (2010) 0.96