Re-examination of the charge relay system in subtilisin comparison with other serine proteases.

Molecular mechanisms for the conversion of zymogens to active proteolytic enzymes. Protein Sci (1998) 2.52

Current problems in mechanistic studies of serine and cysteine proteinases. Biochem J (1982) 1.71

Evidence for a two-state transition in papain that may have no close analogue in ficin. Differences in the disposition of cationic sites and hydrophobic binding areas in the active centres of papain and ficin. Biochem J (1980) 1.39

Mutagenesis of essential functional residues in acetylcholinesterase. Proc Natl Acad Sci U S A (1990) 1.23

Interactive computer surface graphics approach to study of the active site of bovine trypsin. Proc Natl Acad Sci U S A (1978) 1.08

Do cleavages of amides by serine proteases occur through a stepwise pathway involving tetrahedral intermediates? Proc Natl Acad Sci U S A (1979) 1.02

Catalytic mechanism of serine proteases: reexamination of the pH dependence of the histidyl 1J13C2-H coupling constant in the catalytic triad of alpha-lytic protease. Proc Natl Acad Sci U S A (1981) 1.01

Confirmation of the assignment of the low-field proton resonance of serine proteases by using specifically nitrogen-15 labeled enzyme. Proc Natl Acad Sci U S A (1985) 1.00

Evidence that the lack of high catalytic activity of thiolsubtilisin towards specific substrates may be due to an inappropriately located proton-distribution system. Demonstration of highly nucleophilic character of the thiol group of thiolsubtilisin in the catalytically relevant ionization state of the active centre by use of a two-protonic-state reactivity probe. Biochem J (1981) 0.94

Stereoelectronic control in peptide bond formation. Ab initio calculations and speculations on the mechanism of action of serine proteases. Biophys J (1984) 0.76

Structures of ternary complexes of rat DNA polymerase beta, a DNA template-primer, and ddCTP. Science (1994) 5.57

Loop and subdomain movements in the mechanism of Escherichia coli dihydrofolate reductase: crystallographic evidence. Biochemistry (1997) 5.46

Crystal structure of the ribonuclease H domain of HIV-1 reverse transcriptase. Science (1991) 5.42

Serine proteases: structure and mechanism of catalysis. Annu Rev Biochem (1977) 5.37

Crystal structure of a complex between electron transfer partners, cytochrome c peroxidase and cytochrome c. Science (1992) 4.82

Crystal structures of human DNA polymerase beta complexed with gapped and nicked DNA: evidence for an induced fit mechanism. Biochemistry (1997) 4.55

Role of a buried acid group in the mechanism of action of chymotrypsin. Nature (1969) 4.36

Crystal structure of rat DNA polymerase beta: evidence for a common polymerase mechanism. Science (1994) 4.11

Structure of subtilisin BPN' at 2.5 angström resolution. Nature (1969) 3.98

Crystal structures of Escherichia coli and Lactobacillus casei dihydrofolate reductase refined at 1.7 A resolution. I. General features and binding of methotrexate. J Biol Chem (1982) 3.84

Structure of crystalline -chymotrypsin. V. The atomic structure of tosyl- -chymotrypsin at 2 A resolution. J Mol Biol (1972) 3.09

The 2.6-A crystal structure of Pseudomonas putida cytochrome P-450. J Biol Chem (1985) 2.95

Dihydrofolate reductase: x-ray structure of the binary complex with methotrexate. Science (1977) 2.88

The mental health of patients with a chief complaint of chronic fatigue. A prospective evaluation and follow-up. Arch Intern Med (1988) 2.85

Crystal structure of yeast cytochrome c peroxidase refined at 1.7-A resolution. J Biol Chem (1984) 2.56

Low-resolution electron-density and anomalous-scattering-density maps of Chromatium high-potential iron protein. J Mol Biol (1968) 2.46

Crystal structure of avian dihydrofolate reductase containing phenyltriazine and NADPH. J Biol Chem (1982) 2.45

The stereochemistry of peroxidase catalysis. J Biol Chem (1980) 2.35

Depression and somatization in the chronic fatigue syndrome. Am J Med (1991) 2.33

Crystal structures of human DNA polymerase beta complexed with DNA: implications for catalytic mechanism, processivity, and fidelity. Biochemistry (1996) 2.25

Chymotrypsinogen: 2.5-angstrom crystal structure, comparison with alpha-chymotrypsin, and implications for zymogen activation. Biochemistry (1970) 2.23

Subtilisin; a stereochemical mechanism involving transition-state stabilization. Biochemistry (1972) 2.13

The frequency of the chronic fatigue syndrome in patients with symptoms of persistent fatigue. Ann Intern Med (1988) 2.11

Dihydrofolate reductase from Lactobacillus casei. X-ray structure of the enzyme methotrexate.NADPH complex. J Biol Chem (1978) 1.94

A comparison of Fe 4 S 4 clusters in high-potential iron protein and in ferredoxin. Proc Natl Acad Sci U S A (1972) 1.93

Directed mutagenesis of dihydrofolate reductase. Science (1983) 1.88

Crystal structure of unliganded Escherichia coli dihydrofolate reductase. Ligand-induced conformational changes and cooperativity in binding. Biochemistry (1991) 1.86

Enzyme-DNA interactions required for efficient nucleotide incorporation and discrimination in human DNA polymerase beta. J Biol Chem (1996) 1.85

Synaptic plasma membranes from rat brain synaptosomes: isolation and partial characterization. Biochim Biophys Acta (1971) 1.84

X-ray crystallographic study of boronic acid adducts with subtilisin BPN' (Novo). A model for the catalytic transition state. J Biol Chem (1975) 1.82

Identification of variable domains of the attachment (G) protein of subgroup A respiratory syncytial viruses. J Gen Virol (1991) 1.79

The structure of oxidized cytochrome c 2 of Rhodospirillum rubrum. J Biol Chem (1973) 1.73

Analysis of respiratory syncytial virus strain variation in successive epidemics in one city. J Clin Microbiol (1994) 1.72

Crystal structures of Escherichia coli dihydrofolate reductase: the NADP+ holoenzyme and the folate.NADP+ ternary complex. Substrate binding and a model for the transition state. Biochemistry (1990) 1.71

Amino acid sequence homology among the 2-hydroxy acid dehydrogenases: mitochondrial and cytoplasmic malate dehydrogenases form a homologous system with lactate dehydrogenase. Proc Natl Acad Sci U S A (1982) 1.69

Functional role of aspartic acid-27 in dihydrofolate reductase revealed by mutagenesis. Science (1986) 1.68

Atomic co-ordinates for tosyl-alpha-chymotrypsin. Biochem Biophys Res Commun (1969) 1.67

Crystal structures of recombinant human dihydrofolate reductase complexed with folate and 5-deazafolate. Biochemistry (1990) 1.66

What makes the patient-doctor relationship therapeutic? Exploring the connexional dimension of medical care. Ann Intern Med (1988) 1.64

Crystal structure of rat liver dihydropteridine reductase. Proc Natl Acad Sci U S A (1992) 1.63

In vitro antiviral activity of AG7088, a potent inhibitor of human rhinovirus 3C protease. Antimicrob Agents Chemother (1999) 1.63

The splicing factor-associated protein, p32, regulates RNA splicing by inhibiting ASF/SF2 RNA binding and phosphorylation. EMBO J (1999) 1.63

Refined crystal structures of Escherichia coli and chicken liver dihydrofolate reductase containing bound trimethoprim. J Biol Chem (1985) 1.62

Siderophore presence in sputa of cystic fibrosis patients. Infect Immun (1991) 1.61

Crystal structures of Escherichia coli and Lactobacillus casei dihydrofolate reductase refined at 1.7 A resolution. II. Environment of bound NADPH and implications for catalysis. J Biol Chem (1982) 1.61

Orbital rhabdomyosarcoma. An analysis of 62 cases. Am J Ophthalmol (1966) 1.60

Alpha-delta sleep in patients with a chief complaint of chronic fatigue. South Med J (1994) 1.55

Structure-based design, synthesis, and biological evaluation of irreversible human rhinovirus 3C protease inhibitors. 4. Incorporation of P1 lactam moieties as L-glutamine replacements. J Med Chem (1999) 1.55

Two-Angstrom crystal structure of oxidized Chromatium high potential iron protein. J Biol Chem (1974) 1.55

Structure of and kinetic channelling in bifunctional dihydrofolate reductase-thymidylate synthase. Nat Struct Biol (1994) 1.47

The low yield of physical examinations and laboratory investigations of patients with chronic fatigue. Am J Med Sci (1990) 1.47

A structural basis for metal ion mutagenicity and nucleotide selectivity in human DNA polymerase beta. Biochemistry (1996) 1.45

A hypothetical model of the cytochrome c peroxidase . cytochrome c electron transfer complex. J Biol Chem (1980) 1.44

An x-ray crystallographic study of the binding of peptide chloromethyl ketone inhibitors to subtilisin BPN'. Biochemistry (1972) 1.44

A proposed model for interaction of polypeptides with RNA. Proc Natl Acad Sci U S A (1974) 1.39

Atomic coordinates for subtilisin BPN' (or Novo). Biochem Biophys Res Commun (1971) 1.37

Structure of the human cytomegalovirus protease catalytic domain reveals a novel serine protease fold and catalytic triad. Cell (1996) 1.37

An electron microscopic study of lesion-induced synaptogenesis in the dentate gyrus of the adult rat. I. Magnitude and time course of degeneration. Brain Res (1976) 1.36

Structural bases for function in cytochromes c. An interpretation of comparative x-ray and biochemical data. J Biol Chem (1973) 1.36

The crystal structure of cytochrome c peroxidase. J Biol Chem (1980) 1.35

Single amino acid substitutions dissociate fibrinogen-clotting and thrombomodulin-binding activities of human thrombin. Proc Natl Acad Sci U S A (1991) 1.35

Analysis of relatedness of subgroup A respiratory syncytial viruses isolated worldwide. Virus Res (1992) 1.30

The presence of a histidine-aspartic acid pair in the active site of 2-hydroxyacid dehydrogenases. X-ray refinement of cytoplasmic malate dehydrogenase. J Biol Chem (1983) 1.30

Structure of the iron-sulfur cluster in the Chromatius iron protein at 2.25 Angstrom resolution. Cold Spring Harb Symp Quant Biol (1972) 1.30

Crystal structure of a novel trimethoprim-resistant dihydrofolate reductase specified in Escherichia coli by R-plasmid R67. Biochemistry (1986) 1.29

Structure and biology of tissue factor pathway inhibitor. Thromb Haemost (2001) 1.29

"How much do you get paid if I volunteer?" Suggested institutional policy on reward, consent, and research. Hosp Health Serv Adm (1994) 1.26

Polypeptide halomethyl ketones bind to serine proteases as analogs of the tetrahedral intermediate. X-ray crystallographic comparison of lysine- and phenylalanine-polypeptide chloromethyl ketone-inhibited subtilisin. J Biol Chem (1976) 1.25

Conservation of amino acids in human rhinovirus 3C protease correlates with broad-spectrum antiviral activity of rupintrivir, a novel human rhinovirus 3C protease inhibitor. Antimicrob Agents Chemother (2005) 1.25

Determinants of protein thermostability observed in the 1.9-A crystal structure of malate dehydrogenase from the thermophilic bacterium Thermus flavus. Biochemistry (1993) 1.24

Tripeptide aldehyde inhibitors of human rhinovirus 3C protease: design, synthesis, biological evaluation, and cocrystal structure solution of P1 glutamine isosteric replacements. J Med Chem (1998) 1.24

Electrostatic channeling in the bifunctional enzyme dihydrofolate reductase-thymidylate synthase. J Mol Biol (1996) 1.23

An engineered disulfide bond in dihydrofolate reductase. Biochemistry (1987) 1.20