Published in Biochemistry on September 21, 2012
The C2 domains of otoferlin, dysferlin, and myoferlin alter the packing of lipid bilayers. Biochemistry (2013) 0.89
Molecular underpinnings of synaptic vesicle pool heterogeneity. Traffic (2015) 0.85
Differential Membrane Binding Mechanics of Synaptotagmin Isoforms Observed in Atomic Detail. Biochemistry (2016) 0.77
Lateral diffusion of proteins on supported lipid bilayers: additive friction of synaptotagmin 7 C2A-C2B tandem domains. Biochemistry (2014) 0.76
The C2 domains of granuphilin are high-affinity sensors for plasma membrane lipids. Chem Phys Lipids (2013) 0.76
Membrane Docking of the Synaptotagmin 7 C2A Domain: Electron Paramagnetic Resonance Measurements Show Contributions from Two Membrane Binding Loops. Biochemistry (2015) 0.76
Exceptionally tight membrane-binding may explain the key role of the synaptotagmin-7 C2A domain in asynchronous neurotransmitter release. Proc Natl Acad Sci U S A (2017) 0.75
The Pfam protein families database. Nucleic Acids Res (2011) 33.46
Phosphoinositides in cell regulation and membrane dynamics. Nature (2006) 14.00
Membrane lipids: where they are and how they behave. Nat Rev Mol Cell Biol (2008) 13.77
Molecular anatomy of a trafficking organelle. Cell (2006) 11.47
Experimentally determined hydrophobicity scale for proteins at membrane interfaces. Nat Struct Biol (1996) 8.57
Membrane recognition by phospholipid-binding domains. Nat Rev Mol Cell Biol (2008) 7.43
Multiple, distinct forms of bovine and human protein kinase C suggest diversity in cellular signaling pathways. Science (1986) 6.10
Microdomains of high calcium concentration in a presynaptic terminal. Science (1992) 5.09
Calcium dependence of the rate of exocytosis in a synaptic terminal. Nature (1994) 4.77
The C2 domain calcium-binding motif: structural and functional diversity. Protein Sci (1996) 4.20
How does synaptotagmin trigger neurotransmitter release? Annu Rev Biochem (2008) 4.20
Membrane-protein interactions in cell signaling and membrane trafficking. Annu Rev Biophys Biomol Struct (2005) 3.97
Ca(2+)-dependent and -independent activities of neural and non-neural synaptotagmins. Nature (1995) 3.85
Unraveling the mechanisms of synaptotagmin and SNARE function in neurotransmitter release. Trends Cell Biol (2006) 2.84
Synaptotagmin-mediated bending of the target membrane is a critical step in Ca(2+)-regulated fusion. Cell (2009) 2.47
Electrostatic control of the membrane targeting of C2 domains. Mol Cell (2002) 2.18
Solution structures of the Ca2+-free and Ca2+-bound C2A domain of synaptotagmin I: does Ca2+ induce a conformational change? Biochemistry (1998) 2.11
Genetically encoded short peptide tag for versatile protein labeling by Sfp phosphopantetheinyl transferase. Proc Natl Acad Sci U S A (2005) 2.03
Effect of trehalose on protein structure. Protein Sci (2009) 1.97
Synaptotagmins form a hierarchy of exocytotic Ca(2+) sensors with distinct Ca(2+) affinities. EMBO J (2002) 1.88
Three distinct kinetic groupings of the synaptotagmin family: candidate sensors for rapid and delayed exocytosis. Proc Natl Acad Sci U S A (2005) 1.88
Mechanism of phospholipid binding by the C2A-domain of synaptotagmin I. Biochemistry (1998) 1.76
Synaptotagmin VII as a plasma membrane Ca(2+) sensor in exocytosis. Neuron (2001) 1.76
Molecular mechanism of the synaptotagmin-SNARE interaction in Ca2+-triggered vesicle fusion. Nat Struct Mol Biol (2010) 1.64
Position of synaptotagmin I at the membrane interface: cooperative interactions of tandem C2 domains. Biochemistry (2006) 1.54
Role of aromatic side chains in the folding and thermodynamic stability of integral membrane proteins. J Am Chem Soc (2007) 1.53
Ca2+-triggered simultaneous membrane penetration of the tandem C2-domains of synaptotagmin I. Biophys J (2006) 1.52
Ca2+-signaling cycle of a membrane-docking C2 domain. Biochemistry (1997) 1.51
Identification of synaptotagmin effectors via acute inhibition of secretion from cracked PC12 cells. J Cell Biol (2003) 1.51
GRP1 pleckstrin homology domain: activation parameters and novel search mechanism for rare target lipid. Biochemistry (2004) 1.48
Specific translocation of protein kinase Calpha to the plasma membrane requires both Ca2+ and PIP2 recognition by its C2 domain. Mol Biol Cell (2005) 1.47
Membrane-bound orientation and position of the synaptotagmin I C2A domain by site-directed spin labeling. Biochemistry (2003) 1.47
The molecular basis of differential subcellular localization of C2 domains of protein kinase C-alpha and group IVa cytosolic phospholipase A2. J Biol Chem (2003) 1.44
Mechanism of specific membrane targeting by C2 domains: localized pools of target lipids enhance Ca2+ affinity. Biochemistry (2007) 1.42
Synaptotagmin gene content of the sequenced genomes. BMC Genomics (2004) 1.41
C2 domains of protein kinase C isoforms alpha, beta, and gamma: activation parameters and calcium stoichiometries of the membrane-bound state. Biochemistry (2002) 1.39
The Ca2+ affinity of synaptotagmin 1 is markedly increased by a specific interaction of its C2B domain with phosphatidylinositol 4,5-bisphosphate. J Biol Chem (2009) 1.38
The C2B domain of synaptotagmin I is a Ca2+-binding module. Biochemistry (2001) 1.38
Differential but convergent functions of Ca2+ binding to synaptotagmin-1 C2 domains mediate neurotransmitter release. Proc Natl Acad Sci U S A (2009) 1.37
A new phosphatidylinositol 4,5-bisphosphate-binding site located in the C2 domain of protein kinase Calpha. J Biol Chem (2002) 1.37
Structure of human synaptotagmin 1 C2AB in the absence of Ca2+ reveals a novel domain association. Biochemistry (2007) 1.36
Single molecule diffusion of membrane-bound proteins: window into lipid contacts and bilayer dynamics. Biophys J (2010) 1.35
Synaptotagmin-7 is a principal Ca2+ sensor for Ca2+ -induced glucagon exocytosis in pancreas. J Physiol (2009) 1.34
C2 domains from different Ca2+ signaling pathways display functional and mechanistic diversity. Biochemistry (2001) 1.33
Membrane-docking loops of the cPLA2 C2 domain: detailed structural analysis of the protein-membrane interface via site-directed spin-labeling. Biochemistry (2003) 1.28
Synaptotagmin VII is targeted to secretory organelles in PC12 cells, where it functions as a high-affinity calcium sensor. Mol Cell Biol (2005) 1.24
Synaptotagmin III/VII isoforms mediate Ca2+-induced insulin secretion in pancreatic islet beta -cells. J Biol Chem (2000) 1.24
Solution and membrane-bound conformations of the tandem C2A and C2B domains of synaptotagmin 1: Evidence for bilayer bridging. J Mol Biol (2009) 1.17
Phosphatidyl-L-serine is necessary for protein kinase C's high-affinity interaction with diacylglycerol-containing membranes. Biochemistry (1994) 1.16
Synaptotagmins bind calcium to release insulin. Am J Physiol Endocrinol Metab (2008) 1.14
Synaptotagmin VII splice variants alpha, beta, and delta are expressed in pancreatic beta-cells and regulate insulin exocytosis. FASEB J (2007) 1.12
Independent folding and ligand specificity of the C2 calcium-dependent lipid binding domain of cytosolic phospholipase A2. J Biol Chem (1998) 1.11
Membrane penetration by synaptotagmin is required for coupling calcium binding to vesicle fusion in vivo. J Neurosci (2011) 1.04
Lipid specific activation of the murine P4-ATPase Atp8a1 (ATPase II). Biochemistry (2006) 1.03
Hydrophobic interaction chromatography of proteins. J Biotechnol (2001) 1.02
Release mode of large and small dense-core vesicles specified by different synaptotagmin isoforms in PC12 cells. Mol Biol Cell (2011) 0.98
The calcium binding loops of the cytosolic phospholipase A2 C2 domain specify targeting to Golgi and ER in live cells. Mol Biol Cell (2003) 0.97
Synaptotagmin 1 modulates lipid acyl chain order in lipid bilayers by demixing phosphatidylserine. J Biol Chem (2011) 0.95
Use of fluorescence resonance energy transfer to monitor Ca(2+)-triggered membrane docking of C2 domains. Methods Mol Biol (2002) 0.95
Role of electrostatic and hydrophobic interactions in Ca(2+)-dependent phospholipid binding by the C(2)A-domain from synaptotagmin I. Diabetes (2002) 0.94
Mechanism of the calcium-dependent multimerization of synaptotagmin VII mediated by its first and second C2 domains. J Biol Chem (2001) 0.93
Role of C2 domain proteins during synaptic vesicle exocytosis. Biochem Soc Trans (2010) 0.92
Post-translational modifications and lipid binding profile of insect cell-expressed full-length mammalian synaptotagmin 1. Biochemistry (2011) 0.91
Assembly of membrane-bound protein complexes: detection and analysis by single molecule diffusion. Biochemistry (2012) 0.90
Differential role of the Ca(2+) sensor synaptotagmin VII in macrophages and dendritic cells. Immunobiology (2009) 0.90
Nutrient modulation of polarized and sustained submembrane Ca2+ microgradients in mouse pancreatic islet cells. J Physiol (2000) 0.87
The cooperative response of synaptotagmin I C2A. A hypothesis for a Ca2+-driven molecular hammer. Biophys J (2006) 0.84
Distinct roles of the C2A and the C2B domain of the vesicular Ca2+ sensor synaptotagmin 9 in endocrine beta-cells. Biochem J (2007) 0.80
Protein kinase A-dependent phosphorylation of B/K protein. Exp Mol Med (2006) 0.78
Bacterial chemoreceptors: high-performance signaling in networked arrays. Trends Biochem Sci (2007) 5.28
Evidence that opioids may have toll-like receptor 4 and MD-2 effects. Brain Behav Immun (2009) 2.19
Ca2+ influx is an essential component of the positive-feedback loop that maintains leading-edge structure and activity in macrophages. Proc Natl Acad Sci U S A (2007) 1.81
Structure of the conserved HAMP domain in an intact, membrane-bound chemoreceptor: a disulfide mapping study. Biochemistry (2007) 1.80
Use of site-directed cysteine and disulfide chemistry to probe protein structure and dynamics: applications to soluble and transmembrane receptors of bacterial chemotaxis. Methods Enzymol (2007) 1.58
Membrane orientation and position of the C2 domain from cPLA2 by site-directed spin labeling. Biochemistry (2002) 1.57
Engineered socket study of signaling through a four-helix bundle: evidence for a yin-yang mechanism in the kinase control module of the aspartate receptor. Biochemistry (2009) 1.51
Adaptation mechanism of the aspartate receptor: electrostatics of the adaptation subdomain play a key role in modulating kinase activity. Biochemistry (2005) 1.51
Side chains at the membrane-water interface modulate the signaling state of a transmembrane receptor. Biochemistry (2004) 1.49
CheA Kinase of bacterial chemotaxis: chemical mapping of four essential docking sites. Biochemistry (2006) 1.49
GRP1 pleckstrin homology domain: activation parameters and novel search mechanism for rare target lipid. Biochemistry (2004) 1.48
Specific translocation of protein kinase Calpha to the plasma membrane requires both Ca2+ and PIP2 recognition by its C2 domain. Mol Biol Cell (2005) 1.47
The core signaling proteins of bacterial chemotaxis assemble to form an ultrastable complex. Biochemistry (2009) 1.46
Conserved glycine residues in the cytoplasmic domain of the aspartate receptor play essential roles in kinase coupling and on-off switching. Biochemistry (2005) 1.44
Single-molecule fluorescence studies of a PH domain: new insights into the membrane docking reaction. Biophys J (2009) 1.44
Mechanism of specific membrane targeting by C2 domains: localized pools of target lipids enhance Ca2+ affinity. Biochemistry (2007) 1.42
C2 domain of protein kinase C alpha: elucidation of the membrane docking surface by site-directed fluorescence and spin labeling. Biochemistry (2003) 1.40
C2 domains of protein kinase C isoforms alpha, beta, and gamma: activation parameters and calcium stoichiometries of the membrane-bound state. Biochemistry (2002) 1.39
Quantitative analysis of aspartate receptor signaling complex reveals that the homogeneous two-state model is inadequate: development of a heterogeneous two-state model. J Mol Biol (2003) 1.38
Single molecule diffusion of membrane-bound proteins: window into lipid contacts and bilayer dynamics. Biophys J (2010) 1.35
Molecular mechanism of an oncogenic mutation that alters membrane targeting: Glu17Lys modifies the PIP lipid specificity of the AKT1 PH domain. Biochemistry (2008) 1.30
Membrane-docking loops of the cPLA2 C2 domain: detailed structural analysis of the protein-membrane interface via site-directed spin-labeling. Biochemistry (2003) 1.28
Formation of a copper specific binding site in non-native states of beta-2-microglobulin. Biochemistry (2002) 1.28
Use of EPR power saturation to analyze the membrane-docking geometries of peripheral proteins: applications to C2 domains. Annu Rev Biophys Biomol Struct (2005) 1.23
Mapping out regions on the surface of the aspartate receptor that are essential for kinase activation. Biochemistry (2003) 1.17
Evidence that the adaptation region of the aspartate receptor is a dynamic four-helix bundle: cysteine and disulfide scanning studies. Biochemistry (2005) 1.15
Self-induced docking site of a deeply embedded peripheral membrane protein. Biophys J (2006) 1.14
Effect of PIP2 binding on the membrane docking geometry of PKC alpha C2 domain: an EPR site-directed spin-labeling and relaxation study. Biochemistry (2008) 1.07
Membrane docking geometry and target lipid stoichiometry of membrane-bound PKCα C2 domain: a combined molecular dynamics and experimental study. J Mol Biol (2010) 1.06
The 3.2 Å resolution structure of a receptor: CheA:CheW signaling complex defines overlapping binding sites and key residue interactions within bacterial chemosensory arrays. Biochemistry (2013) 1.05
Chemotaxis receptor complexes: from signaling to assembly. PLoS Comput Biol (2007) 1.04
The piston rises again. Structure (2009) 1.04
The PICM chemical scanning method for identifying domain-domain and protein-protein interfaces: applications to the core signaling complex of E. coli chemotaxis. Methods Enzymol (2007) 0.95
Use of fluorescence resonance energy transfer to monitor Ca(2+)-triggered membrane docking of C2 domains. Methods Mol Biol (2002) 0.95
New insights into bacterial chemoreceptor array structure and assembly from electron cryotomography. Biochemistry (2014) 0.94
Defining a key receptor-CheA kinase contact and elucidating its function in the membrane-bound bacterial chemosensory array: a disulfide mapping and TAM-IDS Study. Biochemistry (2013) 0.94
Lateral diffusion of peripheral membrane proteins on supported lipid bilayers is controlled by the additive frictional drags of (1) bound lipids and (2) protein domains penetrating into the bilayer hydrocarbon core. Chem Phys Lipids (2013) 0.93
The GRP1 PH domain, like the AKT1 PH domain, possesses a sentry glutamate residue essential for specific targeting to plasma membrane PI(3,4,5)P(3). Biochemistry (2011) 0.93
Thermal domain motions of CheA kinase in solution: Disulfide trapping reveals the motional constraints leading to trans-autophosphorylation. Biochemistry (2009) 0.92
Assembly of membrane-bound protein complexes: detection and analysis by single molecule diffusion. Biochemistry (2012) 0.90
Isolated bacterial chemosensory array possesses quasi- and ultrastable components: functional links between array stability, cooperativity, and order. Biochemistry (2012) 0.89
Single-molecule studies reveal a hidden key step in the activation mechanism of membrane-bound protein kinase C-α. Biochemistry (2014) 0.88
Molecular mechanism of membrane binding of the GRP1 PH domain. J Mol Biol (2013) 0.87
Ca2+ activation of the cPLA2 C2 domain: ordered binding of two Ca2+ ions with positive cooperativity. Biochemistry (2004) 0.86
Chemotaxis receptors and signaling. Adv Protein Chem (2004) 0.85
Membrane docking geometry of GRP1 PH domain bound to a target lipid bilayer: an EPR site-directed spin-labeling and relaxation study. PLoS One (2012) 0.84
Structure, function, and on-off switching of a core unit contact between CheA kinase and CheW adaptor protein in the bacterial chemosensory array: A disulfide mapping and mutagenesis study. Biochemistry (2013) 0.84
Increasing and decreasing the ultrastability of bacterial chemotaxis core signaling complexes by modifying protein-protein contacts. Biochemistry (2014) 0.81
Zar1 represses translation in Xenopus oocytes and binds to the TCS in maternal mRNAs with different characteristics than Zar2. Biochim Biophys Acta (2013) 0.81
The PH domain of phosphoinositide-dependent kinase-1 exhibits a novel, phospho-regulated monomer-dimer equilibrium with important implications for kinase domain activation: single-molecule and ensemble studies. Biochemistry (2013) 0.80
Interactions of protein kinase C-α C1A and C1B domains with membranes: a combined computational and experimental study. J Am Chem Soc (2014) 0.80
Cation charge and size selectivity of the C2 domain of cytosolic phospholipase A(2). Biochemistry (2002) 0.78
OS-FRET: a new one-sample method for improved FRET measurements. Biochemistry (2010) 0.76
Lateral diffusion of proteins on supported lipid bilayers: additive friction of synaptotagmin 7 C2A-C2B tandem domains. Biochemistry (2014) 0.76
Reprint of: Purification of Proteins Using Polyhistidine Affinity Tags. Protein Expr Purif (2011) 0.75