Published in Methods Enzymol on January 01, 1979
Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6 kilobase pairs. Genetics (1993) 6.47
Intrinsic GTPase activity distinguishes normal and oncogenic ras p21 molecules. Proc Natl Acad Sci U S A (1984) 6.45
Physical and genetic characterization of the glnA--glnG region of the Escherichia coli chromosome. Proc Natl Acad Sci U S A (1981) 5.63
Molecular characterization of a fimbrial adhesin, F1845, mediating diffuse adherence of diarrhea-associated Escherichia coli to HEp-2 cells. J Bacteriol (1989) 4.37
Characterization of a gene, glnL, the product of which is involved in the regulation of nitrogen utilization in Escherichia coli. J Bacteriol (1982) 4.19
Isolation and nucleotide sequence determination of a gene encoding a heat-stable enterotoxin of Escherichia coli. Infect Immun (1983) 4.14
Somatic cells efficiently join unrelated DNA segments end-to-end. Mol Cell Biol (1982) 4.13
Properties of plasmids responsible for production of extended-spectrum beta-lactamases. Antimicrob Agents Chemother (1991) 3.90
LcrD, a membrane-bound regulator of the Yersinia pestis low-calcium response. J Bacteriol (1991) 3.43
Identification and cloning of a hemin storage locus involved in the pigmentation phenotype of Yersinia pestis. J Bacteriol (1990) 3.09
Cloning of a restriction-modification system from Proteus vulgaris and its use in analyzing a methylase-sensitive phenotype in Escherichia coli. J Bacteriol (1985) 2.92
Cloning of plasmid genes encoding resistance to cadmium, zinc, and cobalt in Alcaligenes eutrophus CH34. J Bacteriol (1987) 2.86
Isolation and sequence of the gene for the large subunit of ribulose-1,5-bisphosphate carboxylase from the cyanobacterium Anabaena 7120. Proc Natl Acad Sci U S A (1983) 2.82
Isolation and characterization of the Streptococcus mutans gtfC gene, coding for synthesis of both soluble and insoluble glucans. Infect Immun (1988) 2.75
Coliphage P1-mediated transduction of cloned DNA from Escherichia coli to Myxococcus xanthus: use for complementation and recombinational analyses. J Bacteriol (1983) 2.73
Site-specific DNA condensation and pairing mediated by the int protein of bacteriophage lambda. Proc Natl Acad Sci U S A (1982) 2.67
A single base change in the Shine-Dalgarno region of 16S rRNA of Escherichia coli affects translation of many proteins. Proc Natl Acad Sci U S A (1987) 2.62
Determination of the complete nucleotide sequence of the Sendai virus genome RNA and the predicted amino acid sequences of the F, HN and L proteins. Nucleic Acids Res (1986) 2.60
Analysis of an activatable promoter: sequences in the simian virus 40 late promoter required for T-antigen-mediated trans activation. Mol Cell Biol (1985) 2.55
Identification and cloning of a fur regulatory gene in Yersinia pestis. J Bacteriol (1991) 2.55
Nucleotide sequence of the 5' noncoding region and part of the gag gene of Rous sarcoma virus. J Virol (1982) 2.53
Regulation at the glnL-operator-promoter of the complex glnALG operon of Escherichia coli. J Bacteriol (1983) 2.52
Mutational analysis of the redox-sensitive transcriptional regulator OxyR: regions important for oxidation and transcriptional activation. J Bacteriol (1995) 2.44
A low-Ca2+ response operon encodes the V antigen of Yersinia pestis. Infect Immun (1986) 2.39
The yopM gene of Yersinia pestis encodes a released protein having homology with the human platelet surface protein GPIb alpha. J Bacteriol (1989) 2.37
Fragments from both termini of the herpes simplex virus type 1 genome contain signals required for the encapsidation of viral DNA. Nucleic Acids Res (1983) 2.37
Genetic analysis of the antifungal activity of a soilborne Pseudomonas aureofaciens strain. Appl Environ Microbiol (1991) 2.32
Yersinia pestis pH 6 antigen: genetic, biochemical, and virulence characterization of a protein involved in the pathogenesis of bubonic plague. Infect Immun (1990) 2.30
The Yersinia pestis V antigen is a regulatory protein necessary for Ca2(+)-dependent growth and maximal expression of low-Ca2+ response virulence genes. J Bacteriol (1991) 2.30
Development of anti-pp60src serum with antigen produced in Escherichia coli. J Virol (1983) 2.26
Complete nucleotide sequence of the influenza A/PR/8/34 virus NS gene and comparison with the NS genes of the A/Udorn/72 and A/FPV/Rostock/34 strains. Nucleic Acids Res (1980) 2.25
Conservation of the 2,4-diacetylphloroglucinol biosynthesis locus among fluorescent Pseudomonas strains from diverse geographic locations. Appl Environ Microbiol (1996) 2.25
DNA sequence comparison between two tissue-specific variants of the autonomous parvovirus, minute virus of mice. Nucleic Acids Res (1985) 2.24
An animal source for the ROB-1 beta-lactamase of Haemophilus influenzae type b. Antimicrob Agents Chemother (1986) 2.21
A mutation in the 530 loop of Escherichia coli 16S ribosomal RNA causes resistance to streptomycin. Nucleic Acids Res (1988) 2.13
Cloning of the glutamine synthetase I gene from Rhizobium meliloti. J Bacteriol (1983) 2.13
lcrH, a gene necessary for virulence of Yersinia pestis and for the normal response of Y. pestis to ATP and calcium. Infect Immun (1989) 2.12
Isolation, characterization, and DNA sequence analysis of an AAC(6')-II gene from Pseudomonas aeruginosa. Antimicrob Agents Chemother (1989) 2.08
Cloning, expression, and mapping of the Staphylococcus aureus alpha-hemolysin determinant in Escherichia coli K-12. Infect Immun (1983) 2.03
Structure of Marek's disease virus DNA: detailed restriction enzyme map. J Virol (1984) 2.03
Cloning and characterization of the Flavobacterium johnsoniae (Cytophaga johnsonae) gliding motility gene, gldA. Proc Natl Acad Sci U S A (1997) 2.01
Gene escape model: transfer of heavy metal resistance genes from Escherichia coli to Alcaligenes eutrophus on agar plates and in soil samples. Appl Environ Microbiol (1990) 2.00
A recB recC sbcB recJ host prevents recA-independent deletions in recombinant cosmid DNA propagated in Escherichia coli. J Bacteriol (1989) 1.90
Mutational analysis of the redox-sensitive transcriptional regulator OxyR: regions important for DNA binding and multimerization. J Bacteriol (1995) 1.89
Determination of the promoter strength in the mixed transcription system: promoters of lactose, tryptophan and ribosomal protein L10 operons from Escherichia coli. Nucleic Acids Res (1983) 1.88
Use of chromosomal integration in the establishment and expression of blaZ, a Staphylococcus aureus beta-lactamase gene, in Bacillus subtilis. J Bacteriol (1984) 1.87
The apocytochrome b gene in maize mitochondria does not contain introns and is preceded by a potential ribosome binding site. EMBO J (1984) 1.87
blaI and blaR1 regulate beta-lactamase and PBP 2a production in methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother (1993) 1.86
Human U1 RNA genes contain an unusually sensitive nuclease S1 cleavage site within the conserved 3' flanking region. Proc Natl Acad Sci U S A (1984) 1.86
Two independent peroxisomal targeting signals in catalase A of Saccharomyces cerevisiae. J Cell Biol (1993) 1.86
Development of techniques for the genetic manipulation of the gliding bacterium Cytophaga johnsonae. J Bacteriol (1996) 1.81
Cloning and heterologous expression of glycosidase genes from Saccharomyces cerevisiae. Proc Natl Acad Sci U S A (1987) 1.80
Cloning of a phosphate-regulated hemolysin gene (phospholipase C) from Pseudomonas aeruginosa. J Bacteriol (1982) 1.76
Thiol-beta-lactamase: replacement of the active-site serine of RTEM beta-lactamase by a cysteine residue. Proc Natl Acad Sci U S A (1982) 1.74
Plasmid-determined immunity of Escherichia coli K-12 to colicin Ia Is mediated by a plasmid-encoded membrane protein. J Bacteriol (1981) 1.73
Transcription initiation site of rat ribosomal DNA. Nucleic Acids Res (1982) 1.72
Effect of intron mutations on processing and function of Saccharomyces cerevisiae SUP53 tRNA in vitro and in vivo. Mol Cell Biol (1986) 1.71
Construction of a potential live oral bivalent vaccine for typhoid fever and cholera-Escherichia coli-related diarrheas. Infect Immun (1984) 1.70
Immunological and physicochemical characterization of heat-labile enterotoxins isolated from two strains of Escherichia coli. Infect Immun (1982) 1.70
Yersinia pestis YopM: thrombin binding and overexpression. Infect Immun (1992) 1.69
Molecular cloning of the Escherichia coli O75X adhesin. Infect Immun (1987) 1.67
Characterization and expression of the Escherichia coli Mrr restriction system. J Bacteriol (1991) 1.67
Nucleotide sequence of the aacC2 gene, a gentamicin resistance determinant involved in a hospital epidemic of multiply resistant members of the family Enterobacteriaceae. Antimicrob Agents Chemother (1989) 1.66
Nucleotide sequence of a segment of human DNA containing the three tRNA genes. Nucleic Acids Res (1982) 1.65
Construction of plasmids for expression of Rous sarcoma virus transforming protein, p60src, in Escherichia coli. Proc Natl Acad Sci U S A (1982) 1.63
Identification of pilR, which encodes a transcriptional activator of the Pseudomonas aeruginosa pilin gene. J Bacteriol (1992) 1.62
The cloning and expression of the aroL gene from Escherichia coli K12. Purification and complete amino acid sequence of shikimate kinase II, the aroL-gene product. Biochem J (1986) 1.60
Alginate synthesis in Pseudomonas aeruginosa: environmental regulation of the algC promoter. J Bacteriol (1992) 1.58
Cloning and expression of the Pst I restriction-modification system in Escherichia coli. Proc Natl Acad Sci U S A (1981) 1.57
Genetic transfer by conjugation in the thermophilic green sulfur bacterium Chlorobium tepidum. J Bacteriol (1995) 1.57
Structural analysis of plasmid pSN2 in Staphylococcus aureus: no involvement in enterotoxin B production. J Bacteriol (1982) 1.57
Invasion of epithelial cells by Yersinia pestis: evidence for a Y. pestis-specific invasin. Infect Immun (2000) 1.56
Bacillus subtilis sucrose-specific enzyme II of the phosphotransferase system: expression in Escherichia coli and homology to enzymes II from enteric bacteria. Proc Natl Acad Sci U S A (1987) 1.56
Identification of cDNA clones encoding a precursor of rat liver cathepsin B. Proc Natl Acad Sci U S A (1985) 1.55
Transposon insertions in the Flavobacterium johnsoniae ftsX gene disrupt gliding motility and cell division. J Bacteriol (2000) 1.53
Mapping of export signals of Pseudomonas aeruginosa pilin with alkaline phosphatase fusions. J Bacteriol (1987) 1.53
E. coli host strains significantly affect the quality of small scale plasmid DNA preparations used for sequencing. Nucleic Acids Res (1993) 1.53
Overproduction and nucleotide sequence of the respiratory D-lactate dehydrogenase of Escherichia coli. J Bacteriol (1985) 1.51
cDNA cloning of portions of the bacteriophage phi 6 genome. J Bacteriol (1985) 1.51
Escherichia coli F41 adhesin: genetic organization, nucleotide sequence, and homology with the K88 determinant. J Bacteriol (1988) 1.51
Transcription mapping of the Escherichia coli chromosome by electron microscopy. J Bacteriol (1989) 1.50
Structure and regulation of the Yersinia pestis yscBCDEF operon. J Bacteriol (1992) 1.50
Transcriptional and translational analysis of a strongly expressed early region of the vaccinia virus genome. J Virol (1984) 1.49
Cloning and characterization of a gene (fadR) involved in regulation of fatty acid metabolism in Escherichia coli. J Bacteriol (1985) 1.46
A soluble transcription system derived from purified vaccinia virions. J Virol (1985) 1.45
A human DNA-binding protein is methylation-specific and sequence-specific. Nucleic Acids Res (1986) 1.45
mRNA leader length and initiation codon context determine alternative AUG selection for the yeast gene MOD5. Proc Natl Acad Sci U S A (1991) 1.44
Cloning and characterization of oriL2, a large palindromic DNA replication origin of herpes simplex virus type 2. J Virol (1986) 1.44
Cloning and characterization of the Flavobacterium johnsoniae gliding-motility genes gldB and gldC. J Bacteriol (2000) 1.43
lcrR, a low-Ca2(+)-response locus with dual Ca2(+)-dependent functions in Yersinia pestis. J Bacteriol (1990) 1.41
Effects of multiplicity of infection, bacterial protein synthesis, and growth phase on adhesion to and invasion of human cell lines by Salmonella typhimurium. Infect Immun (1993) 1.41
Development of efficient suicide mechanisms for biological containment of bacteria. Appl Environ Microbiol (1991) 1.40
araB Gene and nucleotide sequence of the araC gene of Erwinia carotovora. J Bacteriol (1985) 1.39
Molecular cloning of the cDNA coding for rat ornithine transcarbamoylase. Proc Natl Acad Sci U S A (1983) 1.38
Mutant feline sarcoma proviruses containing the viral oncogene (v-fes) and either feline or murine control elements. J Virol (1983) 1.38
Synthetic oligodeoxyribonucleotide probes for detecting heat-stable enterotoxin-producing Escherichia coli by DNA colony hybridization. Appl Environ Microbiol (1985) 1.38
Pex14p is a member of the protein linkage map of Pex5p. EMBO J (1997) 1.36
Cloning and characterization of the Flavobacterium johnsoniae gliding motility genes gldD and gldE. J Bacteriol (2001) 1.34
Nucleotide sequence of the bovine parainfluenza 3 virus genome: its 3' end and the genes of NP, P, C and M proteins. Nucleic Acids Res (1987) 1.34
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene (1977) 114.87
Construction of plasmids carrying the cI gene of bacteriophage lambda. Proc Natl Acad Sci U S A (1976) 13.10
Construction and characterization of new cloning vehicles. I. Ampicillin-resistant derivatives of the plasmid pMB9. Gene (1977) 12.93
Construction and characterization of new cloning vehicles. IV. Deletion derivatives of pBR322 and pBR325. Gene (1980) 12.14
Expression in Escherichia coli of a chemically synthesized gene for the hormone somatostatin. Science (1977) 9.28
A general method for the purification of restriction enzymes. Nucleic Acids Res (1978) 7.92
Autoregulation and function of a repressor in bacteriophage lambda. Science (1976) 6.83
Plasmid vector pBR322 and its special-purpose derivatives--a review. Gene (1986) 6.52
Physical and genetic characterization of the glnA--glnG region of the Escherichia coli chromosome. Proc Natl Acad Sci U S A (1981) 5.63
Expression in Escherichia coli of chemically synthesized genes for human insulin. Proc Natl Acad Sci U S A (1979) 4.72
Construction and characterization of new cloning vehicles. VI. Plasmid pBR329, a new derivative of pBR328 lacking the 482-base-pair inverted duplication. Gene (1982) 4.36
Recognition sequences of repressor and polymerase in the operators of bacteriophage lambda. Cell (1975) 4.28
Characterization of a gene, glnL, the product of which is involved in the regulation of nitrogen utilization in Escherichia coli. J Bacteriol (1982) 4.19
Maximizing gene expression on a plasmid using recombination in vitro. Cell (1978) 3.84
Origin of replication of pBR345 plasmid DNA. Proc Natl Acad Sci U S A (1977) 2.40
Pathway engineering for the production of aromatic compounds in Escherichia coli. Nat Biotechnol (1996) 2.36
A cautionary note on the use of certain restriction endonucleases with methylated substrates. Gene (1980) 2.36
Characterizing wild-type and mutant promoters of the tetracycline resistance gene in pBR313. Nucleic Acids Res (1979) 2.23
Genetic and physical studies on the replication of ColE1-type plasmids. Cold Spring Harb Symp Quant Biol (1979) 2.15
A plasmid cloning vehicle allowing a positive selection for inserted fragments. Gene (1980) 2.08
Peptides and genes coding for scorpion toxins that affect ion-channels. Biochimie (2000) 1.50
Determination of the nucleotide sequence for the glutamate synthase structural genes of Escherichia coli K-12. Gene (1987) 1.41
ColE1 hybrid plasmids containing Escherichia coli genes involved in the biosynthesis of glutamate and glutamine. Plasmid (1980) 1.30
Tetracycline resistance determined by pBR322 is mediated by one polypeptide. Gene (1983) 1.27
Characterization of a site-specific restriction endonuclease SphI from Streptomyces phaeochromogenes. Gene (1980) 1.24
Cloning, sequencing, and expression in Ficoll-generated minicells of an Escherichia coli heat-stable enterotoxin gene. Plasmid (1988) 1.18
Characterization of sugar mixtures utilization by an Escherichia coli mutant devoid of the phosphotransferase system. Appl Microbiol Biotechnol (2001) 1.15
A family of removable cassettes designed to obtain antibiotic-resistance-free genomic modifications of Escherichia coli and other bacteria. Gene (2000) 1.14
The role of penicillin amidases in nature and in industry. Trends Biochem Sci (1991) 1.13
Complete nucleotide sequence of the glutamate dehydrogenase gene from Escherichia coli K-12. Gene (1984) 1.12
Controllable alteration of cell genotype in bacterial cultures using an excision vector. Gene (1988) 1.12
A prosthetic heart with hemispherical ventricles designed for low hemolytic action. Trans Am Soc Artif Intern Organs (1970) 1.11
Cloning and physical mapping of the glnA gene of Escherichia coli K-12. Gene (1980) 1.05
Tight linkage of genes that encode the two glutamate synthase subunits of Escherichia coli K-12. J Bacteriol (1980) 1.04
The plasmid, pBR322. Biotechnology (1988) 1.02
gltF, a member of the gltBDF operon of Escherichia coli, is involved in nitrogen-regulated gene expression. Mol Microbiol (1992) 1.01
Carbon regulation and the role in nature of the Escherichia coli penicillin acylase (pac) gene. Mol Microbiol (1992) 0.98
pBRINT-Ts: a plasmid family with a temperature-sensitive replicon, designed for chromosomal integration into the lacZ gene of Escherichia coli. Gene (1998) 0.97
Design and construction of expression plasmid vectors in Escherichia coli. Methods Enzymol (1990) 0.94
Identification of a functional promoter for the Escherichia coli gdhA gene and its regulation. Gene (1988) 0.93
Recombinant plasmids carrying the glutamate dehydrogenase structural gene from Escherichia coli K-12. Gene (1982) 0.93
Appendix. Cloning and sequence of the gene encoding enzyme E-1 from the methionine salvage pathway of Klebsiella oxytoca. J Biol Chem (1993) 0.90
Construction of protein overproducer strains in Bacillus subtilis by an integrative approach. Appl Microbiol Biotechnol (2001) 0.90
Structural organization of the genes that encode two glutamate synthase subunits of Escherichia coli. Gene (1983) 0.89
Genetic separability of the chorismate mutase and prephenate dehydrogenase components of the Escherichia coli tyrA gene product. J Bacteriol (1987) 0.89
Repetitive extragenic palindromic (REP) sequences in the Escherichia coli gdhA gene. Gene (1985) 0.87
A common precursor for the two subunits of the penicillin acylase from Escherichia coli ATCC11105. Gene (1985) 0.87
Relationship between codon usage and sequence-dependent curvature of genomes. Microb Comp Genomics (1998) 0.86
Use of phage immunity in molecular cloning experiments. Science (1977) 0.86
Biochemical and genetic characterization of a carbamyl phosphate synthetase mutant of Escherichia coli K12. J Gen Microbiol (1976) 0.85
A pBRINT family of plasmids for integration of cloned DNA into the Escherichia coli chromosome. Gene (1996) 0.84
Laboratory metabolic evolution improves acetate tolerance and growth on acetate of ethanologenic Escherichia coli under non-aerated conditions in glucose-mineral medium. Appl Microbiol Biotechnol (2012) 0.84
Characterization of the regulatory region of the Escherichia coli penicillin acylase structural gene. Gene (1986) 0.83
The ribonucleoside diphosphate reductase gene (nrdA) of Escherichia coli carries a repetitive extragenic palindromic (REP) sequence in its 3' structural terminus. Mol Microbiol (1989) 0.83
Recovery of DNA from agarose gels stained with methylene blue. Biotechniques (1992) 0.82
Amino acid sequence analysis of the glutamate synthase enzyme from Escherichia coli K-12. Protein Seq Data Anal (1989) 0.82
In vivo events in the generation of pBR322. Nucleic Acids Res (1986) 0.81
Identification of a putative hypothalamic mRNA coding for somatostatin and of its product in cell-free translation. Biochem Biophys Res Commun (1980) 0.81
Cloning and characterization of cDNAs that code for Na(+)-channel-blocking toxins of the scorpion Centruroides noxius Hoffmann. Gene (1993) 0.80
Nucleotide sequence of the promoter and amino-terminal coding region of the glutamate dehydrogenase structural gene of Escherichia coli. Gene (1983) 0.80
Relationship between whole proteome aminoacid composition and static DNA curvature. Microb Comp Genomics (2000) 0.79
New insights on the comma-less theory. Orig Life Evol Biosph (1992) 0.78
Recombinant protein production in cultures of an Escherichia coli trp- strain. Appl Microbiol Biotechnol (1993) 0.78
Characterization of the 5' subtilisin (aprE) regulatory region from Bacillus subtilis. FEMS Microbiol Lett (2000) 0.78
The genomic region encoding toxin gamma from the scorpion Tityus serrulatus contains an intron. FEBS Lett (1993) 0.77
Expression in Escherichia coli of a chemically synthesized gene for the hormone somatostatin. 1977. Biotechnology (1992) 0.77
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. 1977. Biotechnology (1992) 0.76
Re: Comparison of DNA probe and ELISA microbial analysis methods and their association with adult periodontitis. J Periodontol (1995) 0.75
Electrostatic effects on polyproline I-II transitions. Biochemistry (1969) 0.75
Production in Escherichia coli of a rat chimeric proinsulin polypeptide carrying human A and B chains and its preparative chromatography. J Biotechnol (1994) 0.75
Synthesis, cloning, and expression of hormone genes in Escherichia coli. Recent Prog Horm Res (1980) 0.75
Development on the implantation of a total nuclear-powered artificial heart system. Trans Am Soc Artif Intern Organs (1974) 0.75
Changing university roles in the century of biotechnology. Nat Biotechnol (1998) 0.75