A general method for the purification of restriction enzymes.

Cloning and characterization of ribosomal RNA genes from wheat and barley. Nucleic Acids Res (1979) 8.75

The use of restriction endonucleases to measure mitochondrial DNA sequence relatedness in natural populations. I. Population structure and evolution in the genus Peromyscus. Genetics (1979) 8.72

Analysis of the nucleotide sequence of an invertible controlling element. Proc Natl Acad Sci U S A (1980) 5.92

Mitochondrial DNA clones and matriarchal phylogeny within and among geographic populations of the pocket gopher, Geomys pinetis. Proc Natl Acad Sci U S A (1979) 5.24

Expression in Escherichia coli of chemically synthesized genes for human insulin. Proc Natl Acad Sci U S A (1979) 4.72

Sequence specific cleavage of DNA by micrococcal nuclease. Nucleic Acids Res (1981) 4.32

Characterization of Tn904 insertions in octopine Ti plasmid mutants of Agrobacterium tumefaciens. J Bacteriol (1980) 4.14

Introduction of pAM beta 1 into Listeria monocytogenes by conjugation and homology between native L. monocytogenes plasmids. Infect Immun (1984) 3.98

Sequence organization of the repeating units in the nucleus of wheat which contain 5S rRNA genes. Nucleic Acids Res (1980) 3.83

RNA polymerase III transcriptional units are interspersed among human non-alpha-globin genes. Proc Natl Acad Sci U S A (1979) 3.78

Nucleotide sequence of the Tn10 encoded tetracycline resistance gene. Nucleic Acids Res (1983) 3.12

Molecular cloning of TOL genes xylB and xylE in Escherichia coli. J Bacteriol (1981) 3.08

Nucleotide sequence of mouse satellite DNA. Nucleic Acids Res (1981) 3.04

The evolutionarily conserved repetitive sequence d(TG.AC)n promotes reciprocal exchange and generates unusual recombinant tetrads during yeast meiosis. Mol Cell Biol (1986) 2.86

Structural analysis of interspersed repetitive polymerase III transcription units in human DNA. Nucleic Acids Res (1981) 2.77

Structural and functional analysis of cloned deoxyribonucleic acid containing the trpR-thr region of the Escherichia coli chromosome. J Bacteriol (1979) 2.65

The legumin gene family: structure of a B type gene of Vicia faba and a possible legumin gene specific regulatory element. Nucleic Acids Res (1986) 2.63

Nuclease-hypersensitive sites in the chromatin domain of the chicken lysozyme gene. Nucleic Acids Res (1983) 2.48

Structure of the constant and 3' untranslated regions of the murine Balb/c gamma 2a heavy chain messenger RNA. Nucleic Acids Res (1980) 2.37

Characterizing wild-type and mutant promoters of the tetracycline resistance gene in pBR313. Nucleic Acids Res (1979) 2.23

Complete sequence of pSC101. Nucleic Acids Res (1984) 2.22

Sequence specificity of exonuclease III from E. coli. Nucleic Acids Res (1982) 2.17

Nucleotide sequence of the structural gene for colicin E1 and predicted structure of the protein. Proc Natl Acad Sci U S A (1982) 2.15

Identification of Campylobacter coli isolates from animals and humans by bacterial restriction endonuclease DNA analysis. Appl Environ Microbiol (1984) 2.15

Identification of the in vivo and in vitro origin of transcription in human rDNA. Nucleic Acids Res (1982) 2.11

Organization of mouse mammary tumor virus-specific DNA endogenous to BALB/c mice. J Virol (1979) 2.11

DNA adenine methylation of GATC sequences appeared recently in the Escherichia coli lineage. J Bacteriol (1984) 2.05

DNA-mediated gene transfer of a circular plasmid into murine cells. Proc Natl Acad Sci U S A (1979) 2.02

Nucleotide sequence of the region of an origin of replication of the antibiotic resistance plasmid R6K. Proc Natl Acad Sci U S A (1979) 1.95

Mouse immunoglobulin genes: a bacterial plasmid containing the entire coding sequence for a pre-gamma 2a heavy chain. Nucleic Acids Res (1980) 1.92

Organization of the lexA gene of Escherichia coli and nucleotide sequence of the regulatory region. Nucleic Acids Res (1981) 1.90

The cohering telomeres of Oxytricha. Nucleic Acids Res (1987) 1.87

Strains and clones of Trypanosoma cruzi can be characterized by pattern of restriction endonuclease products of kinetoplast DNA minicircles. Proc Natl Acad Sci U S A (1980) 1.78

Investigation of Homologous Crossing over and Sister Chromatid Exchange in the Wheat Nor-B2 Locus Coding for Rrna and Gli-B2 Locus Coding for Gliadins. Genetics (1986) 1.73

The EcoRI restriction endonuclease with bacteriophage lambda DNA. Kinetic studies. Biochem J (1980) 1.67

Characterization of a R plasmid-associated, trimethoprim-resistant dihydrofolate reductase and determination of the nucleotide sequence of the reductase gene. Nucleic Acids Res (1981) 1.63

Structural and functional analysis of cloned DNA containing genes responsible for branched-chain amino acid transport in Escherichia coli. Proc Natl Acad Sci U S A (1980) 1.57

Lac repressor - lac operator interaction. Circular dichroism study. Nucleic Acids Res (1981) 1.56

Amino-terminal sequence and processing of the precursor of the leucine-specific binding protein, and evidence for conformational differences between the precursor and the mature form. Proc Natl Acad Sci U S A (1980) 1.56

Comparison of mouse immunoglobulin gamma 2a and gamma 2b chain genes suggests that exons can be exchanged between genes in a multigenic family. Proc Natl Acad Sci U S A (1981) 1.55

Eight different highly specific nucleosome phases on alpha-satellite DNA in the African green monkey. Nucleic Acids Res (1983) 1.52

The genes coding for the muscle contractile proteins, myosin heavy chain, myosin light chain 2, and skeletal muscle actin are located on three different mouse chromosomes. EMBO J (1982) 1.44

Purification and characterization of the deoR repressor of Escherichia coli. EMBO J (1989) 1.42

DNAase I sensitivity of genes expressed during myogenesis. Nucleic Acids Res (1982) 1.42

Aspartate-specific peptidases in Salmonella typhimurium: mutants deficient in peptidase E. J Bacteriol (1984) 1.42

A sequence-specific endonuclease (Xmn I) from Xanthomonas manihotis. Nucleic Acids Res (1980) 1.38

Mouse heavy chain variable regions: nucleotide sequence of a germ-line VH gene segment. Nucleic Acids Res (1981) 1.38

Nucleotide sequences at the ends of the mercury resistance transposon, Tn501. Nucleic Acids Res (1980) 1.37

Nucleotide sequence of the thrA gene of Escherichia coli. Proc Natl Acad Sci U S A (1980) 1.36

Molecular analysis of chromosome-mediated gene transfer. Proc Natl Acad Sci U S A (1979) 1.31

Comparison of T-DNA oncogene complements of Agrobacterium tumefaciens tumor-inducing plasmids with limited and wide host ranges. J Bacteriol (1984) 1.30

Proviruses of mouse mammary tumor virus in normal and neoplastic tissues from GR and C3Hf mouse strains. J Virol (1980) 1.29

Conformational flexibility of junctions between contiguous B- and Z-DNAs in supercoiled plasmids. Proc Natl Acad Sci U S A (1983) 1.28

Non-specific binding of restriction endonuclease EcoR1 to DNA. Nucleic Acids Res (1980) 1.28

Methylation of the chicken vitellogenin gene: influence of estradiol administration. Nucleic Acids Res (1983) 1.19

Molecular structure and function of the bacteriocin gene and bacteriocin protein of plasmid Clo DF13. Nucleic Acids Res (1983) 1.16

Genetic study of plasmid-associated zonal resistance to lincomycin in Streptococcus pyogenes. Antimicrob Agents Chemother (1981) 1.11

Plasmid replication functions: two distinct segments of plasmid R1, RepA and RepD, express incompatibility and are capable of autonomous replication. J Bacteriol (1980) 1.11

Nucleotide sequence of constant and 3' untranslated regions of a kappa immunoglobulin light chain mRNA of a homozygous b4 rabbit. Proc Natl Acad Sci U S A (1981) 1.08

Accessibility of expressed and non-expressed genes to a restriction nuclease. Nucleic Acids Res (1980) 1.08

Isolation and computer-aided characterization of MmeI, a type II restriction endonuclease from Methylophilus methylotrophus. Nucleic Acids Res (1986) 1.08

Analysis of highly purified satellite DNA containing chromatin from the mouse. Nucleic Acids Res (1982) 1.05

Benzpyrene groups bind preferentially to the DNA of active chromatin in human lung cells. Nucleic Acids Res (1982) 1.05

Tight linkage of genes that encode the two glutamate synthase subunits of Escherichia coli K-12. J Bacteriol (1980) 1.04

A new restriction endonuclease from the anaerobic bacterium, Desulfovibrio desulfuricans, Norway. Nucleic Acids Res (1980) 1.02

Isolation and Mapping of Small Cauliflower Mosaic Virus DNA Fragments Active as Promoters in Escherichia coli. J Virol (1981) 1.02

Analysis of mini-F plasmid replication by transposition mutagenesis. J Bacteriol (1981) 1.02

Recombination between bacteriophage lambda and plasmid pBR322 in Escherichia coli. J Bacteriol (1980) 1.01

Analysis of hemolytic determinants of plasmid pHly185 by Tn5 mutagenesis. J Bacteriol (1982) 1.01

E coli RNA polymerase-rRNA promoter interaction and the effect of ppGpp. Nucleic Acids Res (1980) 0.98

Vaccinia virus encodes a polypeptide with DNA ligase activity. Nucleic Acids Res (1989) 0.98

Molecular cloning of rabbit gamma heavy chain mRNA. Nucleic Acids Res (1982) 0.97

The genes coding for the cardiac muscle actin, the skeletal muscle actin and the cytoplasmic beta-actin are located on three different mouse chromosomes. EMBO J (1983) 0.97

Integration site of polyoma virus DNA in the inducible LPT line of polyoma-transformed rat cells. J Virol (1982) 0.97

The SalGI restriction endonuclease. Purification and properties. Biochem J (1982) 0.96

Circular dichroism studies of the B goes to A conformational transition in seven small DNA restriction fragments containing the Escherichia coli lactose control region. Nucleic Acids Res (1980) 0.96

Molecular cloning of pheR in Escherichia coli K-12. J Bacteriol (1982) 0.95

Molecular epidemiology of DNA viruses: applications of restriction endonuclease cleavage site analysis. Yale J Biol Med (1980) 0.93

Elevated levels of petite formation in strains of Saccharomyces cerevisiae restored to respiratory competence. I. Association of both high and moderate frequencies of petite mutant formation with the presence of aberrant mitochondrial DNA. Genetics (1985) 0.93

Purification of the mRNA for chicken very low density lipoproteinII and molecular cloning of its full-length double-stranded cDNA. Nucleic Acids Res (1979) 0.92

Anti-self receptors. V. Properties of a mouse serum factor that blocks autorosetting receptors on lymphocytes. Immunology (1982) 0.88

Elevated levels of petite formation in strains of Saccharomyces cerevisiae restored to respiratory competence. II. Organization of mitochondrial genomes in strains having high and moderate frequencies of petite mutant formation. Genetics (1985) 0.88

A comparison of DNA cleavage by the restriction enzymes SalPI and PstI. Nucleic Acids Res (1980) 0.87

Unambiguous typing of canine adenovirus isolates by deoxyribonucleic acid restriction-endonuclease analysis. Can J Comp Med (1983) 0.86

Bacteriophage T7 late promoters: construction and in vitro transcription properties of deletion mutants. Nucleic Acids Res (1982) 0.86

Electrospray ionization mass spectrometric characterization of photocrosslinked DNA-EcoRI DNA methyltransferase complexes. Nucleic Acids Res (1998) 0.86

Non-additivity of sequence-specific enzyme-DNA interactions in the EcoRI DNA methyltransferase. Nucleic Acids Res (1991) 0.85

Analysis of bacteriophage SP82 major "early" in vitro transcripts. J Virol (1981) 0.85

Evidence indicating that the cholera toxin structural genes of Vibrio cholerae RJ1 and 3083-2 are between met and trp. Infect Immun (1983) 0.82

A novel exocytoplasmic endonuclease from Streptomyces antibioticus. Biochem J (1995) 0.81

Correlation of thermodynamic and genetic properties in the Tn10 encoded TET gene control region. Nucleic Acids Res (1982) 0.81

Leu-enkephalin purification from E. coli cells carrying the plasmid with fused synthetic leu-enkephalin gene. Nucleic Acids Res (1980) 0.79

Structure and regulation of gene expression of a Clo DF13 plasmid DNA region involved in plasmid segregation and incompatibility. Nucleic Acids Res (1983) 0.78

Assessment of plasmid profile, exoenzyme activity, and virulence in recent human isolates of Yersinia enterocolitica. J Clin Microbiol (1985) 0.77

Purification and characterization of a nutritionally controlled endodeoxyribonuclease from Streptomyces glaucescens. Biochem J (1992) 0.77

Editorial: NAR surveys the past, present and future of restriction endonucleases. Nucleic Acids Res (2014) 0.76

Bacteriophages of methanotrophs isolated from fish. Appl Environ Microbiol (1983) 0.75

Interaction of the tight-binding I12-X86 lac repressor with non operator DNA: salt dependence of complex formation. Nucleic Acids Res (1986) 0.75

Analysis of tet operator-TET repressor complexes by thermal denaturation studies. Nucleic Acids Res (1982) 0.75

Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene (1977) 114.87

Restriction endonucleases. CRC Crit Rev Biochem (1976) 13.22

Restriction endonucleases in the analysis and restructuring of dna molecules. Annu Rev Biochem (1975) 11.76

Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene (1977) 114.87

A complementation analysis of the restriction and modification of DNA in Escherichia coli. J Mol Biol (1969) 71.21

Plasmid ColEl as a molecular vehicle for cloning and amplification of DNA. Proc Natl Acad Sci U S A (1974) 32.46

Construction of biologically functional bacterial plasmids in vitro. Proc Natl Acad Sci U S A (1973) 25.61

Analysis of endonuclease R-EcoRI fragments of DNA from lambdoid bacteriophages and other viruses by agarose-gel electrophoresis. J Virol (1974) 23.54

Construction and characterization of new cloning vehicles. III. Derivatives of plasmid pBR322 carrying unique Eco RI sites for selection of Eco RI generated recombinant DNA molecules. Gene (1978) 17.08

DNA nucleotide sequence restricted by the RI endonuclease. Proc Natl Acad Sci U S A (1972) 15.71

Construction of plasmids carrying the cI gene of bacteriophage lambda. Proc Natl Acad Sci U S A (1976) 13.10

Construction and characterization of new cloning vehicles. I. Ampicillin-resistant derivatives of the plasmid pMB9. Gene (1977) 12.93

Construction and characterization of new cloning vehicles. IV. Deletion derivatives of pBR322 and pBR325. Gene (1980) 12.14

Plasmids of Escherichia coli as cloning vectors. Methods Enzymol (1979) 11.41

Specificity of substrate recognition by the EcoRI restriction endonuclease. Proc Natl Acad Sci U S A (1975) 11.07

Replication and transcription of eukaryotic DNA in Escherichia coli. Proc Natl Acad Sci U S A (1974) 10.51

Expression in Escherichia coli of a chemically synthesized gene for the hormone somatostatin. Science (1977) 9.28

Protein expression in E. coli minicells by recombinant plasmids. Cell (1977) 8.74

Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides. Gene (1995) 7.42

Characterization of a mini-ColC1 plasmid. J Bacteriol (1976) 7.20

Ligation of EcoRI endonuclease-generated DNA fragments into linear and circular structures. J Mol Biol (1975) 6.95

Autoregulation and function of a repressor in bacteriophage lambda. Science (1976) 6.83

Plasmid vector pBR322 and its special-purpose derivatives--a review. Gene (1986) 6.52

DNA restriction and modification mechanisms in bacteria. Annu Rev Microbiol (1971) 6.05

DNA substrate site for the EcoRII restriction endonuclease and modification methylase. Nat New Biol (1973) 5.90

Human leukocyte interferon produced by E. coli is biologically active. Nature (1980) 5.86

Physical and genetic characterization of the glnA--glnG region of the Escherichia coli chromosome. Proc Natl Acad Sci U S A (1981) 5.63

Synthetic lac operator DNA is functional in vivo. Nature (1976) 5.36

Restriction and modification of DNA: enzymes and substrates. Introductory remarks. Fed Proc (1974) 5.31

Cloning and expression of human tissue-type plasminogen activator cDNA in E. coli. Nature (1983) 5.27

The Escherichia coli B restriction endonuclease. Biochim Biophys Acta (1969) 5.00

Structure of the DNA-Eco RI endonuclease recognition complex at 3 A resolution. Science (1986) 4.89

The rapid purification of T4 DNA ligase from a lambda T4 lig lysogen. J Biol Chem (1980) 4.82

Chemical synthesis of restriction enzyme recognition sites useful for cloning. Science (1977) 4.81

Expression in Escherichia coli of chemically synthesized genes for human insulin. Proc Natl Acad Sci U S A (1979) 4.72

Physical identity of the SV40 deoxyribonucleic acid sequence recognized by the Eco RI restriction endonuclease and modification methylase. Biochemistry (1974) 4.58

Construction and characterization of new cloning vehicles. VI. Plasmid pBR329, a new derivative of pBR328 lacking the 482-base-pair inverted duplication. Gene (1982) 4.36

Recognition sequences of repressor and polymerase in the operators of bacteriophage lambda. Cell (1975) 4.28

Restriction and modification of a self-complementary octanucleotide containing the EcoRI substrate. J Mol Biol (1975) 4.21

Characterization of a gene, glnL, the product of which is involved in the regulation of nitrogen utilization in Escherichia coli. J Bacteriol (1982) 4.19

Construction and characterization of the chloramphenicol-resistance gene cartridge: a new approach to the transcriptional mapping of extrachromosomal elements. Gene (1982) 3.99

Cloning, nucleotide sequence, and expression in Escherichia coli of the exotoxin A structural gene of Pseudomonas aeruginosa. Proc Natl Acad Sci U S A (1984) 3.98

Maximizing gene expression on a plasmid using recombination in vitro. Cell (1978) 3.84

Direct expression in Escherichia coli of a DNA sequence coding for human growth hormone. Nature (1979) 3.80

Letter: Potential biohazards of recombinant DNA molecules. Science (1974) 3.45

A restriction endonuclease analysis of the bacterial plasmid controlling the ecoRI restriction and modification of DNA. Fed Proc (1976) 3.04

Altered tetracycline resistance in pSC101 recombinant plasmids. Mol Gen Genet (1977) 3.03

On the nature of tetracycline resistance controlled by the plasmid pSC101. Cell (1978) 2.80

Refinement of Eco RI endonuclease crystal structure: a revised protein chain tracing. Science (1990) 2.74

Construction and characterization of new cloning vehicles. V. Mobilization and coding properties of pBR322 and several deletion derivatives including pBR327 and pBR328. Gene (1981) 2.72

R factor-controlled restriction and modification of deoxyribonucleic acid: restriction mutants. J Bacteriol (1972) 2.65

Sequence analysis of the DNA encoding the Eco RI endonuclease and methylase. J Biol Chem (1981) 2.62

The aminoglycoside-resistance operon of the plasmid pSa: nucleotide sequence of the streptomycin-spectinomycin resistance gene. Gene (1985) 2.51

Kinked DNA in crystalline complex with EcoRI endonuclease. Nature (1984) 2.46

Origin of replication of pBR345 plasmid DNA. Proc Natl Acad Sci U S A (1977) 2.40

Characterization of a halobacterial gene affecting bacterio-opsin gene expression. Nucleic Acids Res (1984) 2.37

Pathway engineering for the production of aromatic compounds in Escherichia coli. Nat Biotechnol (1996) 2.36

A cautionary note on the use of certain restriction endonucleases with methylated substrates. Gene (1980) 2.36

Characterizing wild-type and mutant promoters of the tetracycline resistance gene in pBR313. Nucleic Acids Res (1979) 2.23

Classification and characterization of the rice alpha-amylase multigene family. Plant Mol Biol (1990) 2.20

Genetic and physical studies on the replication of ColE1-type plasmids. Cold Spring Harb Symp Quant Biol (1979) 2.15

Interaction of bacteriophage T4 RNA and DNA ligases in joining of duplex DNA at base-paired ends. J Biol Chem (1977) 2.13

A plasmid cloning vehicle allowing a positive selection for inserted fragments. Gene (1980) 2.08

The structures and fidelity of replication of mouse mitochondrial DNA-pSC 101 EcoRI recombinant plasmids grown in E. coli K12. Cell (1976) 2.02

Highly hydrophilic proteins in prokaryotes and eukaryotes are common during conditions of water deficit. J Biol Chem (2000) 1.99

Biosynthesis of bacterial glycogen. Cloning of the glycogen biosynthetic enzyme structural genes of Escherichia coli. J Biol Chem (1981) 1.93

Bacterio-opsin mutants of Halobacterium halobium. Proc Natl Acad Sci U S A (1983) 1.91

Expression of Tn9-derived chloramphenicol resistance in Bacillus subtilis. Nature (1981) 1.90

Construction and characterization of E. coli promoter-probe plasmid vectors. I. Cloning of promoter-containing DNA fragments. Gene (1979) 1.87

Construction and characterization of E. coli promoter-probe plasmid vectors. III. pBR322 derivatives with deletions in the tetracycline resistance promoter region. Gene (1982) 1.81

Production of two proteolytic enzymes by a transformable strain of Bacillus subtilis. Arch Biochem Biophys (1968) 1.74

The complete nucleotide sequence of the glnALG operon of Escherichia coli K12. Nucleic Acids Res (1987) 1.73

Molecular cloning of an Escherichia coli plasmid determinant than encodes for the production of heat-stable enterotoxin. J Bacteriol (1976) 1.69

Identification of new RNA modifying enzymes by iterative genome search using known modifying enzymes as probes. Nucleic Acids Res (1996) 1.68

Genetic control of the secondary modification of deoxyribonucleic acid in Escherichia coli. J Bacteriol (1970) 1.63

RAmy2A; a novel alpha-amylase-encoding gene in rice. Gene (1992) 1.60

Targeted random mutagenesis: the use of ambiguously synthesized oligonucleotides to mutagenize sequences immediately 5' of an ATG initiation codon. Nucleic Acids Res (1983) 1.60

Characterization of insertions affecting the expression of the bacterio-opsin gene in Halobacterium halobium. Nucleic Acids Res (1984) 1.59

Expression of the bop gene cluster of Halobacterium halobium is induced by low oxygen tension and by light. J Bacteriol (1991) 1.51