Published in Methods Enzymol on January 01, 1979
Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti. Proc Natl Acad Sci U S A (1980) 45.02
Use of synthetic oligonucleotides as hybridization probes: isolation of cloned cDNA sequences for human beta 2-microglobulin. Proc Natl Acad Sci U S A (1981) 7.24
Regions of broad-host-range plasmid RK2 which are essential for replication and maintenance. J Bacteriol (1980) 5.45
Cloning and sequencing of the class B beta-lactamase gene (ccrA) from Bacteroides fragilis TAL3636. Antimicrob Agents Chemother (1990) 5.24
Molecular cloning of tomato fruit polygalacturonase: Analysis of polygalacturonase mRNA levels during ripening. Proc Natl Acad Sci U S A (1986) 5.09
Physical and genetic map of a Rhizobium meliloti nodulation gene region and nucleotide sequence of nodC. J Bacteriol (1985) 4.98
Territorial limits and functional anatomy of the simian virus 40 replication origin. Proc Natl Acad Sci U S A (1982) 4.77
Suicide plasmid vehicles for insertion mutagenesis in Rhizobium meliloti and related bacteria. J Bacteriol (1983) 4.30
Induction of phenylalanine ammonia-lyase and 4-coumarate:CoA ligase mRNAs in cultured plant cells by UV light or fungal elicitor. Proc Natl Acad Sci U S A (1984) 4.22
Properties of plasmids responsible for production of extended-spectrum beta-lactamases. Antimicrob Agents Chemother (1991) 3.90
Complementation analysis of Agrobacterium tumefaciens Ti plasmid mutations affecting oncogenicity. J Bacteriol (1982) 3.90
Application of DNA-DNA colony hybridization to the detection of catabolic genotypes in environmental samples. Appl Environ Microbiol (1985) 3.82
Localization of an origin of DNA replication within the TRS/IRS repeated region of the herpes simplex virus type 1 genome. EMBO J (1982) 3.61
Expression of a recombinant DNA gene coding for the vesicular stomatitis virus nucleocapsid protein. J Virol (1983) 3.30
Cloning of SHV-2, OHIO-1, and OXA-6 beta-lactamases and cloning and sequencing of SHV-1 beta-lactamase. Antimicrob Agents Chemother (1990) 3.26
Chromosomal replication origin from the marine bacterium Vibrio harveyi functions in Escherichia coli: oriC consensus sequence. Proc Natl Acad Sci U S A (1983) 3.26
Flagellar transcriptional activators FlbB and FlaI: gene sequences and 5' consensus sequences of operons under FlbB and FlaI control. J Bacteriol (1988) 3.24
The 'light' and 'medium' subunits of the photosynthetic reaction centre from Rhodopseudomonas viridis: isolation of the genes, nucleotide and amino acid sequence. EMBO J (1986) 3.23
A multifunctional gene (tetR) controls Tn10-encoded tetracycline resistance. J Bacteriol (1982) 3.19
Primary structure of the M subunit of the reaction center from Rhodopseudomonas sphaeroides. Proc Natl Acad Sci U S A (1983) 2.87
Derivatization of unprotected polynucleotides. Nucleic Acids Res (1983) 2.86
Genetic and physical analyses of a cluster of genes essential for xanthan gum biosynthesis in Xanthomonas campestris. J Bacteriol (1987) 2.83
Adenovirus type 5 early region 1b gene product is required for efficient shutoff of host protein synthesis. J Virol (1984) 2.82
Physical and biochemical analysis of the cloned recB and recC genes of Escherichia coli K-12. J Bacteriol (1984) 2.72
Extension of the host range of Escherichia coli vectors by incorporation of RSF1010 replication and mobilization functions. J Bacteriol (1985) 2.70
Clustering of nitrogen fixation (nif) genes in Rhizobium meliloti. J Bacteriol (1982) 2.66
Broad host range plasmid RK2 encodes multiple kil genes potentially lethal to Escherichia coli host cells. Proc Natl Acad Sci U S A (1982) 2.66
Overproduction and purification of the luxR gene product: Transcriptional activator of the Vibrio fischeri luminescence system. Proc Natl Acad Sci U S A (1987) 2.59
Construction of a cloned library of the EcoRI fragments from the human cytomegalovirus genome (strain AD169). J Virol (1982) 2.52
Nucleotide sequence of the Escherichia coli motB gene and site-limited incorporation of its product into the cytoplasmic membrane. J Bacteriol (1986) 2.51
Cloning determinants of pathogenesis from Pseudomonas syringae pathovar syringae. Proc Natl Acad Sci U S A (1985) 2.50
Promoter mapping and transcriptional regulation of the iron assimilation system of plasmid ColV-K30 in Escherichia coli K-12. J Bacteriol (1985) 2.45
Construction of a Rhizobium japonicum gene bank and use in isolation of a hydrogen uptake gene. Proc Natl Acad Sci U S A (1983) 2.44
Dual role for Escherichia coli RecA protein in SOS mutagenesis. Proc Natl Acad Sci U S A (1985) 2.43
Isolation and characterization of Pseudomonas putida PpF1 mutants defective in the toluene dioxygenase enzyme system. J Bacteriol (1984) 2.41
Cells that constitutively express the herpes simplex virus immediate-early protein ICP4 allow efficient activation of viral delayed-early genes in trans. J Virol (1985) 2.40
DNA repair in Escherichia coli: identification of the uvrD gene product. Proc Natl Acad Sci U S A (1982) 2.40
Role of micF in the tolC-mediated regulation of OmpF, a major outer membrane protein of Escherichia coli K-12. J Bacteriol (1987) 2.39
DNA sequence adjacent to flagellar genes and evolution of flagellar-phase variation. J Bacteriol (1983) 2.30
Nucleotide sequence binding specificity of the LexA repressor of Escherichia coli K-12. J Bacteriol (1985) 2.16
Cloning of the glutamine synthetase I gene from Rhizobium meliloti. J Bacteriol (1983) 2.13
beta-Lactamases and beta-lactam resistance in Escherichia coli. Antimicrob Agents Chemother (1985) 2.09
The ;heavy' subunit of the photosynthetic reaction centre from Rhodopseudomonas viridis: isolation of the gene, nucleotide and amino acid sequence. EMBO J (1985) 2.06
Molecular cloning of human immune interferon cDNA and its expression in eukaryotic cells. Nucleic Acids Res (1982) 2.01
Identification of Escherichia coli region III flagellar gene products and description of two new flagellar genes. J Bacteriol (1984) 1.99
Genetic analysis of a temporally transcribed chemotaxis gene cluster in Caulobacter crescentus. Genetics (1991) 1.96
Molecular Studies on the Role of a Root Surface Agglutinin in Adherence and Colonization by Pseudomonas putida. Appl Environ Microbiol (1988) 1.95
Comparative toxicity and virulence of Escherichia coli clones expressing variant and chimeric Shiga-like toxin type II operons. Infect Immun (1995) 1.93
Isolation and characterization of the gene encoding the principal sigma factor of the vegetative cell RNA polymerase from the cyanobacterium Anabaena sp. strain PCC 7120. J Bacteriol (1991) 1.89
"Transactivation" control signals in the promoter of the herpesvirus thymidine kinase gene. Proc Natl Acad Sci U S A (1985) 1.87
Integration and excision of pMC7105 in Pseudomonas syringae pv. phaseolicola: involvement of repetitive sequences. J Bacteriol (1984) 1.83
Four plasmid genes are required for colicin V synthesis, export, and immunity. J Bacteriol (1987) 1.80
Deletions within the transformation-specific RNA sequences of acute leukemia virus MC29 give rise to partially transformation-defective mutants. J Virol (1982) 1.79
Importance of state of methylation of oriC GATC sites in initiation of DNA replication in Escherichia coli. EMBO J (1985) 1.77
Plasmid-determined immunity of Escherichia coli K-12 to colicin Ia Is mediated by a plasmid-encoded membrane protein. J Bacteriol (1981) 1.73
Structure of the heterogeneous L-S junction region of human cytomegalovirus strain AD169 DNA. J Virol (1984) 1.73
Use of cloned mtl genes of Escherichia coli to introduce mtl deletion mutations into the chromosome. J Bacteriol (1983) 1.72
Characterization of a Genomic Region Required for Production of the Antibiotic Pyoluteorin by the Biological Control Agent Pseudomonas fluorescens Pf-5. Appl Environ Microbiol (1995) 1.71
Cloning of the filamentous hemagglutinin of Bordetella pertussis and its expression in Escherichia coli. Infect Immun (1987) 1.69
Evidence for a unique human fibroblast interferon (IFN-beta 1) chromosomal gene, devoid of intervening sequences. Nucleic Acids Res (1981) 1.67
Direct selection of IS903 transposon insertions by use of a broad-host-range vector: isolation of catalase-deficient mutants of Actinobacillus actinomycetemcomitans. J Bacteriol (1999) 1.66
SopB protein-mediated silencing of genes linked to the sopC locus of Escherichia coli F plasmid. Proc Natl Acad Sci U S A (1995) 1.64
Characterization of Rhizobium japonicum hydrogen uptake genes. J Bacteriol (1984) 1.62
Plasmid-directed synthesis of enzymes required for D-mannitol transport and utilization in Escherichia coli. Proc Natl Acad Sci U S A (1981) 1.60
Cloning of the genes for and characterization of the early stages of toluene and o-xylene catabolism in Pseudomonas stutzeri OX1. Appl Environ Microbiol (1996) 1.59
Polyomavirus large T antigen binds independently to multiple, unique regions on the viral genome. J Virol (1983) 1.55
Duplication insertion of drug resistance determinants in the radioresistant bacterium Deinococcus radiodurans. J Bacteriol (1988) 1.55
Transposon Tn5 specifies streptomycin resistance in Rhizobium spp. J Bacteriol (1984) 1.55
The trfB region of broad host range plasmid RK2: the nucleotide sequence reveals incC and key regulatory gene trfB/korA/korD as overlapping genes. Nucleic Acids Res (1986) 1.54
Chromosomal replication origins (oriC) of Enterobacter aerogenes and Klebsiella pneumoniae are functional in Escherichia coli. J Bacteriol (1982) 1.54
Transformation of Haemophilus influenzae by plasmid RSF0885. J Bacteriol (1981) 1.51
Cloning of genes involved in the biosynthesis of pseudobactin, a high-affinity iron transport agent of a plant growth-promoting Pseudomonas strain. J Bacteriol (1984) 1.51
Simian virus 40 large T-antigen point mutants that are defective in viral DNA replication but competent in oncogenic transformation. Mol Cell Biol (1984) 1.51
Characterization and sequence of Escherichia coli pabC, the gene encoding aminodeoxychorismate lyase, a pyridoxal phosphate-containing enzyme. J Bacteriol (1992) 1.50
In vitro analysis of polypeptide requirements of multicomponent phenol hydroxylase from Pseudomonas sp. strain CF600. J Bacteriol (1990) 1.45
Maintenance and stability of introduced genotypes in groundwater aquifer material. Appl Environ Microbiol (1987) 1.44
Intra- and interspecies transfer and expression of Rhizobium japonicum hydrogen uptake genes and autotrophic growth capability. Proc Natl Acad Sci U S A (1985) 1.43
Map location and nucleotide sequence of korA, a key regulatory gene of promiscuous plasmid RK2. Nucleic Acids Res (1983) 1.43
Cell cycle stage-specific accumulation of mRNAs encoding tubulin and other polypeptides in Chlamydomonas. Proc Natl Acad Sci U S A (1982) 1.43
Polyoma viral middle T-antigen is required for transformation. J Virol (1982) 1.43
Characterization of the penA and penR genes of Burkholderia cepacia 249 which encode the chromosomal class A penicillinase and its LysR-type transcriptional regulator. Antimicrob Agents Chemother (1997) 1.41
ParB of Pseudomonas aeruginosa: interactions with its partner ParA and its target parS and specific effects on bacterial growth. J Bacteriol (2004) 1.39
The signal sequence of the p62 protein of Semliki Forest virus is involved in initiation but not in completing chain translocation. J Cell Biol (1990) 1.38
Essential genes of plasmid RK2 in Escherichia coli: trfB region controls a kil gene near trfA. J Bacteriol (1983) 1.37
Aerobactin iron uptake sequences in plasmid ColV-K30 are flanked by inverted IS1-like elements and replication regions. J Bacteriol (1984) 1.37
Analysis of the gene cluster encoding toluene/o-xylene monooxygenase from Pseudomonas stutzeri OX1. Appl Environ Microbiol (1998) 1.36
Characterization of transposon Tn5086, carrying the site-specifically inserted gene dhfrVII mediating trimethoprim resistance. J Bacteriol (1993) 1.35
Construction and use of a gene bank of Alcaligenes eutrophus in the analysis of ribulose bisphosphate carboxylase genes. J Bacteriol (1984) 1.32
Development of natural and synthetic DNA probes for OXA-2 and TEM-1 beta-lactamases. Antimicrob Agents Chemother (1987) 1.32
Replication patterns of multiple plasmids coexisting in Escherichia coli. J Bacteriol (1988) 1.28
Analysis of the promoter and regulatory sequences of an oxygen-regulated bch operon in Rhodobacter capsulatus by site-directed mutagenesis. J Bacteriol (1993) 1.27
The korB gene of broad host range plasmid RK2 is a major copy number control element which may act together with trfB by limiting trfA expression. EMBO J (1984) 1.27
The korF region of broad-host-range plasmid RK2 encodes two polypeptides with transcriptional repressor activity. J Bacteriol (1991) 1.26
Deletion mutants of the Escherichia coli K-12 mannitol permease: dissection of transport-phosphorylation, phospho-exchange, and mannitol-binding activities. J Bacteriol (1989) 1.26
Efficient transformation of Dictyostelium discoideum amoebae. Mol Cell Biol (1983) 1.25
Replication control in promiscuous plasmid RK2: kil and kor functions affect expression of the essential replication gene trfA. J Bacteriol (1985) 1.24
Isolation of a Legionella pneumophila restriction mutant with increased ability to act as a recipient in heterospecific matings. J Bacteriol (1989) 1.24
korA function of promiscuous plasmid RK2: an autorepressor that inhibits expression of host-lethal gene kilA and replication gene trfA. Proc Natl Acad Sci U S A (1985) 1.23
Tn5supF, a 264-base-pair transposon derived from Tn5 for insertion mutagenesis and sequencing DNAs cloned in phage lambda. Proc Natl Acad Sci U S A (1989) 1.22
Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form. Proc Natl Acad Sci U S A (1969) 71.62
Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti. Proc Natl Acad Sci U S A (1980) 45.02
Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans. Proc Natl Acad Sci U S A (1979) 44.75
Plasmid ColEl as a molecular vehicle for cloning and amplification of DNA. Proc Natl Acad Sci U S A (1974) 32.46
Stimulation by cyclic adenosine monophosphate of plasmid deoxyribonucleic acid replication and catabolite repression of the plasmid deoxyribonucleic acid-protein relaxation complex. J Bacteriol (1973) 30.43
Firefly luciferase gene: structure and expression in mammalian cells. Mol Cell Biol (1987) 26.70
Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event. Biochemistry (1970) 26.22
Circular DNA forms of colicinogenic factors E1, E2 and E3 from Escherichia coli. J Mol Biol (1968) 23.73
The effectiveness of right heart catheterization in the initial care of critically ill patients. SUPPORT Investigators. JAMA (1996) 17.62
Initiation of biofilm formation in Pseudomonas fluorescens WCS365 proceeds via multiple, convergent signalling pathways: a genetic analysis. Mol Microbiol (1998) 17.38
Flagellar and twitching motility are necessary for Pseudomonas aeruginosa biofilm development. Mol Microbiol (1998) 17.18
Characterization of multiple circular DNA forms of colicinogenic factor E-1 from Proteus mirabilis. Biochemistry (1968) 14.82
Biofilm formation as microbial development. Annu Rev Microbiol (2000) 13.33
Plasmids related to the broad host range vector, pRK290, useful for gene cloning and for monitoring gene expression. Plasmid (1985) 11.74
Evidence for circular DNA forms of a bacterial plasmid. Proc Natl Acad Sci U S A (1967) 10.45
Genetic analysis of Escherichia coli biofilm formation: roles of flagella, motility, chemotaxis and type I pili. Mol Microbiol (1998) 10.07
Circular DNA. Annu Rev Biochem (1971) 9.75
Effect of growth conditions on the formation of the relaxation complex of supercoiled ColE1 deoxyribonucleic acid and protein in Escherichia coli. J Bacteriol (1972) 9.50
Meta-analysis of 74,046 individuals identifies 11 new susceptibility loci for Alzheimer's disease. Nat Genet (2013) 9.46
Plasmid vectors for high-efficiency expression controlled by the PL promoter of coliphage lambda. Gene (1981) 9.44
DNA polymerase as a requirement for the maintenance of the bacterial plasmid colicinogenic factor E1. Biochem Biophys Res Commun (1970) 8.73
Circular DNA forms of a bacterial sex factor. Proc Natl Acad Sci U S A (1967) 8.55
Trans-complementation-dependent replication of a low molecular weight origin fragment from plasmid R6K. Cell (1978) 8.28
Fruiting body formation by Bacillus subtilis. Proc Natl Acad Sci U S A (2001) 8.06
Characterization of a mini-ColC1 plasmid. J Bacteriol (1976) 7.20
Effect of Rec mutations on the activity of colicinogenic factors. J Bacteriol (1967) 7.19
Replication of a bacterial plasmid and an episome in Escherichia coli. Biochemistry (1970) 7.19
Genetic approaches to study of biofilms. Methods Enzymol (1999) 6.78
The National Depressive and Manic-Depressive Association consensus statement on the undertreatment of depression. JAMA (1997) 6.32
Treatment of murine colitis by Lactococcus lactis secreting interleukin-10. Science (2000) 6.19
Unidirectional replication of the P-group plasmid RK2. Biochim Biophys Acta (1977) 6.17
Microbial competition: Escherichia coli mutants that take over stationary phase cultures. Science (1993) 6.13
Steps in the development of a Vibrio cholerae El Tor biofilm. Mol Microbiol (1999) 6.06
Transient and stable expression of the firefly luciferase gene in plant cells and transgenic plants. Science (1986) 5.97
Outcomes following acute exacerbation of severe chronic obstructive lung disease. The SUPPORT investigators (Study to Understand Prognoses and Preferences for Outcomes and Risks of Treatments) Am J Respir Crit Care Med (1996) 5.82
Heme biosynthesis in Rhizobium. Identification of a cloned gene coding for delta-aminolevulinic acid synthetase from Rhizobium meliloti. J Biol Chem (1982) 5.80
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Method for the isolation of the replication region of a bacterial replicon: construction of a mini-F'kn plasmid. J Bacteriol (1976) 5.75
Purification and characterization of colicin E2 and colicin E3. J Biol Chem (1967) 5.74
A catenated DNA molecule as an intermediate in the replication of the resistance transfer factor R6K in Escherichia coli. Biochem Biophys Res Commun (1973) 5.47
Regions of broad-host-range plasmid RK2 which are essential for replication and maintenance. J Bacteriol (1980) 5.45
ABC transporters: bacterial exporters. Microbiol Rev (1993) 5.35
Temperature-sensitive mutants for the replication of plasmids in Escherichia coli: requirement for deoxyribonucleic acid polymerase I in the replication of the plasmid ColE 1 . J Bacteriol (1973) 5.24
Gene cloning and containment properties of plasmid Col E1 and its derivatives. Science (1977) 5.21
Comparative study of the events associated with colicin induction. J Bacteriol (1967) 5.14
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A novel DNA-binding protein with regulatory and protective roles in starved Escherichia coli. Genes Dev (1992) 4.96
Twenty five years of case finding and audit in a socially deprived community. BMJ (1991) 4.89
Improved plasmid vectors with a thermoinducible expression and temperature-regulated runaway replication. Gene (1983) 4.87
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Suppression of Co1E1 replication properties by the Inc P-1 plasmid RK2 in hybrid plasmids constructed in vitro. J Mol Biol (1979) 4.69
The DNA-protein relaxation complex of the plasmid RK2: location of the site-specific nick in the region of the proposed origin of transfer. Mol Gen Genet (1979) 4.48
Isolation of supercoiled colicinogenic factor E 1 DNA sensitive to ribonuclease and alkali. Proc Natl Acad Sci U S A (1972) 4.46
A haemoprotein with kinase activity encoded by the oxygen sensor of Rhizobium meliloti. Nature (1991) 4.45
Generation in vitro of deletions in the broad host range plasmid RK2 using phage Mu insertions and a restriction endonuclease. Gene (1976) 4.35
Stationary-phase-inducible "gearbox" promoters: differential effects of katF mutations and role of sigma 70. J Bacteriol (1991) 4.30
Physical and genetic studies with restriction endonucleases on the broad host-range plasmid RK2. Mol Gen Genet (1977) 4.28
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The dps promoter is activated by OxyR during growth and by IHF and sigma S in stationary phase. Mol Microbiol (1994) 3.97
Regions of broad-host-range plasmid RK2 involved in replication and stable maintenance in nine species of gram-negative bacteria. J Bacteriol (1985) 3.93
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From peptide precursors to oxazole and thiazole-containing peptide antibiotics: microcin B17 synthase. Science (1996) 3.74
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Molecular cloning of human interleukin 2 cDNA and its expression in E. coli. Nucleic Acids Res (1983) 3.44
Rhizobium meliloti genes required for nodule development are related to chromosomal virulence genes in Agrobacterium tumefaciens. Proc Natl Acad Sci U S A (1986) 3.38
A role for the mannose-sensitive hemagglutinin in biofilm formation by Vibrio cholerae El Tor. J Bacteriol (1999) 3.38
Cloning of firefly luciferase cDNA and the expression of active luciferase in Escherichia coli. Proc Natl Acad Sci U S A (1985) 3.35
Consensus statement on the live organ donor. JAMA (2000) 3.29
Sensing starvation: a homoserine lactone--dependent signaling pathway in Escherichia coli. Science (1994) 3.27
An E. coli promoter induced by the cessation of growth. Mol Microbiol (1987) 3.26
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The export of the DNA replication inhibitor Microcin B17 provides immunity for the host cell. EMBO J (1988) 3.25
Attenuation in amino acid biosynthetic operons. Annu Rev Genet (1982) 3.23
Structural relationships among Rhizobium meliloti symbiotic promoters. Cell (1983) 3.23
The global carbon metabolism regulator Crc is a component of a signal transduction pathway required for biofilm development by Pseudomonas aeruginosa. J Bacteriol (2000) 3.21
Evolution of microbial diversity during prolonged starvation. Proc Natl Acad Sci U S A (1999) 3.18
Occurrence of myeloproliferative disorder in patients with Noonan syndrome. J Pediatr (1997) 3.14
surA, an Escherichia coli gene essential for survival in stationary phase. J Bacteriol (1990) 3.11
Can the uptake of breast screening by Asian women be increased? A randomized controlled trial of a linkworker intervention. J Public Health Med (1994) 3.04
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Construction of plasmid cloning vehicles that promote gene expression from the bacteriophage lambda pL promoter. Gene (1979) 3.00
Molecular vehicle properties of the broad host range plasmid RK2. Science (1975) 2.97