Published in J Am Chem Soc on November 11, 2013
Unravelling the structural and mechanistic basis of CRISPR-Cas systems. Nat Rev Microbiol (2014) 3.48
The evolutionary journey of Argonaute proteins. Nat Struct Mol Biol (2014) 1.41
Detection and characterization of spacer integration intermediates in type I-E CRISPR-Cas system. Nucleic Acids Res (2014) 1.40
Intrinsic sequence specificity of the Cas1 integrase directs new spacer acquisition. Elife (2015) 1.12
CRISPR-Cas adaptation: insights into the mechanism of action. Nat Rev Microbiol (2016) 1.05
Campylobacter jejuni acquire new host-derived CRISPR spacers when in association with bacteriophages harboring a CRISPR-like Cas4 protein. Front Microbiol (2015) 0.88
Electromagnetic toroidal excitations in matter and free space. Nat Mater (2016) 0.86
The CRISPR-associated Cas4 protein Pcal_0546 from Pyrobaculum calidifontis contains a [2Fe-2S] cluster: crystal structure and nuclease activity. Nucleic Acids Res (2014) 0.82
CRISPR RNA binding and DNA target recognition by purified Cascade complexes from Escherichia coli. Nucleic Acids Res (2014) 0.80
Host adaption to the bacteriophage carrier state of Campylobacter jejuni. Res Microbiol (2015) 0.79
Evolution of an archaeal virus nucleocapsid protein from the CRISPR-associated Cas4 nuclease. Biol Direct (2015) 0.79
The Bacteriophage Carrier State of Campylobacter jejuni Features Changes in Host Non-coding RNAs and the Acquisition of New Host-derived CRISPR Spacer Sequences. Front Microbiol (2016) 0.77
Coot: model-building tools for molecular graphics. Acta Crystallogr D Biol Crystallogr (2004) 227.01
Refinement of macromolecular structures by the maximum-likelihood method. Acta Crystallogr D Biol Crystallogr (1997) 137.43
PHENIX: a comprehensive Python-based system for macromolecular structure solution. Acta Crystallogr D Biol Crystallogr (2010) 108.52
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science (2012) 48.17
CRISPR provides acquired resistance against viruses in prokaryotes. Science (2007) 29.74
Dali server: conservation mapping in 3D. Nucleic Acids Res (2010) 24.29
Small CRISPR RNAs guide antiviral defense in prokaryotes. Science (2008) 17.79
RNA-guided genetic silencing systems in bacteria and archaea. Nature (2012) 17.12
Evolution and classification of the CRISPR-Cas systems. Nat Rev Microbiol (2011) 17.11
The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA. Nature (2010) 14.21
HKL-3000: the integration of data reduction and structure solution--from diffraction images to an initial model in minutes. Acta Crystallogr D Biol Crystallogr (2006) 13.29
A guild of 45 CRISPR-associated (Cas) protein families and multiple CRISPR/Cas subtypes exist in prokaryotic genomes. PLoS Comput Biol (2005) 11.82
A putative RNA-interference-based immune system in prokaryotes: computational analysis of the predicted enzymatic machinery, functional analogies with eukaryotic RNAi, and hypothetical mechanisms of action. Biol Direct (2006) 11.31
RNA-guided RNA cleavage by a CRISPR RNA-Cas protein complex. Cell (2009) 11.12
Short motif sequences determine the targets of the prokaryotic CRISPR defence system. Microbiology (2009) 10.67
Biochemistry of homologous recombination in Escherichia coli. Microbiol Rev (1994) 9.93
MOLPROBITY: structure validation and all-atom contact analysis for nucleic acids and their complexes. Nucleic Acids Res (2004) 9.86
An exonuclease induced by bacteriophage lambda. II. Nature of the enzymatic reaction. J Biol Chem (1967) 5.82
Auto-rickshaw: an automated crystal structure determination platform as an efficient tool for the validation of an X-ray diffraction experiment. Acta Crystallogr D Biol Crystallogr (2005) 4.74
A DNA repair system specific for thermophilic Archaea and bacteria predicted by genomic context analysis. Nucleic Acids Res (2002) 4.39
Crystal structure of RecBCD enzyme reveals a machine for processing DNA breaks. Nature (2004) 4.35
Type II restriction endonucleases: structure and mechanism. Cell Mol Life Sci (2005) 4.15
Dynamic properties of the Sulfolobus CRISPR/Cas and CRISPR/Cmr systems when challenged with vector-borne viral and plasmid genes and protospacers. Mol Microbiol (2010) 3.77
CRISPR-mediated adaptive immune systems in bacteria and archaea. Annu Rev Biochem (2013) 3.55
Metabolomic analysis via reversed-phase ion-pairing liquid chromatography coupled to a stand alone orbitrap mass spectrometer. Anal Chem (2010) 3.22
A novel family of sequence-specific endoribonucleases associated with the clustered regularly interspaced short palindromic repeats. J Biol Chem (2008) 3.00
A dual function of the CRISPR-Cas system in bacterial antivirus immunity and DNA repair. Mol Microbiol (2010) 2.70
The Structural Biology Center 19ID undulator beamline: facility specifications and protein crystallographic results. J Synchrotron Radiat (2005) 2.62
The CRISPRs, they are a-changin': how prokaryotes generate adaptive immunity. Annu Rev Genet (2012) 2.56
Structure and activity of the Cas3 HD nuclease MJ0384, an effector enzyme of the CRISPR interference. EMBO J (2011) 2.47
Nucleases: diversity of structure, function and mechanism. Q Rev Biophys (2010) 2.22
Toroidal structure of lambda-exonuclease. Science (1997) 2.14
Memory of viral infections by CRISPR-Cas adaptive immune systems: acquisition of new information. Virology (2012) 1.80
CRISPR-based immune systems of the Sulfolobales: complexity and diversity. Biochem Soc Trans (2011) 1.66
An iron-sulfur cluster is essential for the binding of broken DNA by AddAB-type helicase-nucleases. J Biol Chem (2009) 1.50
A single nuclease active site of the Escherichia coli RecBCD enzyme catalyzes single-stranded DNA degradation in both directions. J Biol Chem (2000) 1.49
Response of the hyperthermophilic archaeon Sulfolobus solfataricus to UV damage. J Bacteriol (2007) 1.46
Identification of novel restriction endonuclease-like fold families among hypothetical proteins. Nucleic Acids Res (2005) 1.44
Responses of hyperthermophilic crenarchaea to UV irradiation. Genome Biol (2007) 1.41
Characterization of the CRISPR/Cas subtype I-A system of the hyperthermophilic crenarchaeon Thermoproteus tenax. J Bacteriol (2012) 1.35
Microarray analysis of the hyperthermophilic archaeon Pyrococcus furiosus exposed to gamma irradiation. Extremophiles (2006) 1.31
The CRISPR associated protein Cas4 Is a 5' to 3' DNA exonuclease with an iron-sulfur cluster. PLoS One (2012) 1.22
Insights into Chi recognition from the structure of an AddAB-type helicase-nuclease complex. EMBO J (2012) 1.21
Oxidative stress protection and the repair response to hydrogen peroxide in the hyperthermophilic archaeon Pyrococcus furiosus and in related species. Arch Microbiol (2010) 1.13
Formation of a distinctive complex between the inducible bacterial lysine decarboxylase and a novel AAA+ ATPase. J Biol Chem (2005) 1.10
Crystal structures of lambda exonuclease in complex with DNA suggest an electrostatic ratchet mechanism for processivity. Proc Natl Acad Sci U S A (2011) 1.08
Crystal structure of E. coli RecE protein reveals a toroidal tetramer for processing double-stranded DNA breaks. Structure (2009) 0.97
Transcriptional responses of the deep-sea hyperthermophile Methanocaldococcus jannaschii under shifting extremes of temperature and pressure. Extremophiles (2007) 0.85
Structural and functional insight into the mechanism of an alkaline exonuclease from Laribacter hongkongensis. Nucleic Acids Res (2011) 0.81
Evolution and classification of the CRISPR-Cas systems. Nat Rev Microbiol (2011) 17.11
Crystal structure of the extracellular segment of integrin alpha Vbeta3 in complex with an Arg-Gly-Asp ligand. Science (2002) 7.87
A virulence locus of Pseudomonas aeruginosa encodes a protein secretion apparatus. Science (2006) 7.84
Protein production and purification. Nat Methods (2008) 3.97
Structure of a bacterial quorum-sensing transcription factor complexed with pheromone and DNA. Nature (2002) 3.45
Structures of human insulin-degrading enzyme reveal a new substrate recognition mechanism. Nature (2006) 3.21
Crystal structure of the polymerase PA(C)-PB1(N) complex from an avian influenza H5N1 virus. Nature (2008) 3.06
In situ proteolysis for protein crystallization and structure determination. Nat Methods (2007) 3.05
A novel family of sequence-specific endoribonucleases associated with the clustered regularly interspaced short palindromic repeats. J Biol Chem (2008) 3.00
A dual function of the CRISPR-Cas system in bacterial antivirus immunity and DNA repair. Mol Microbiol (2010) 2.70
Crystal structure of the CorA Mg2+ transporter. Nature (2006) 2.64
The Structural Biology Center 19ID undulator beamline: facility specifications and protein crystallographic results. J Synchrotron Radiat (2005) 2.62
An expression vector tailored for large-scale, high-throughput purification of recombinant proteins. Protein Expr Purif (2006) 2.58
Structure and activity of the Cas3 HD nuclease MJ0384, an effector enzyme of the CRISPR interference. EMBO J (2011) 2.47
Outcome of a workshop on archiving structural models of biological macromolecules. Structure (2006) 2.32
A family of LIC vectors for high-throughput cloning and purification of proteins. Methods Mol Biol (2009) 2.30
Rhomboid-7 and HtrA2/Omi act in a common pathway with the Parkinson's disease factors Pink1 and Parkin. Dis Model Mech (2008) 2.29
Crystal structure of the TSP-1 type 1 repeats: a novel layered fold and its biological implication. J Cell Biol (2002) 2.28
Crystal structure of SV40 large T-antigen bound to p53: interplay between a viral oncoprotein and a cellular tumor suppressor. Genes Dev (2006) 2.23
Automation of protein purification for structural genomics. J Struct Funct Genomics (2004) 2.17
Genome-wide analysis of substrate specificities of the Escherichia coli haloacid dehalogenase-like phosphatase family. J Biol Chem (2006) 2.10
Structure of the influenza virus A H5N1 nucleoprotein: implications for RNA binding, oligomerization, and vaccine design. FASEB J (2008) 2.10
Outcome of a workshop on applications of protein models in biomedical research. Structure (2009) 2.05
Nuclease activity of the human SAMHD1 protein implicated in the Aicardi-Goutieres syndrome and HIV-1 restriction. J Biol Chem (2013) 1.95
PoxA, yjeK, and elongation factor P coordinately modulate virulence and drug resistance in Salmonella enterica. Mol Cell (2010) 1.89
Mechanism of histone lysine methyl transfer revealed by the structure of SET7/9-AdoMet. EMBO J (2003) 1.89
Structure of RapA, a Swi2/Snf2 protein that recycles RNA polymerase during transcription. Structure (2008) 1.86
Triclinic lysozyme at 0.65 A resolution. Acta Crystallogr D Biol Crystallogr (2007) 1.77
The HD domain of the Escherichia coli tRNA nucleotidyltransferase has 2',3'-cyclic phosphodiesterase, 2'-nucleotidase, and phosphatase activities. J Biol Chem (2004) 1.66
Structure of the Shigella T3SS effector IpaH defines a new class of E3 ubiquitin ligases. Nat Struct Mol Biol (2008) 1.65
The crystal structure of spermidine synthase with a multisubstrate adduct inhibitor. Nat Struct Biol (2002) 1.63
Identification of unknown protein function using metabolite cocktail screening. Structure (2012) 1.62
Structural proteomics: toward high-throughput structural biology as a tool in functional genomics. Acc Chem Res (2003) 1.62
Enzyme genomics: Application of general enzymatic screens to discover new enzymes. FEMS Microbiol Rev (2005) 1.61
Integrating structure, bioinformatics, and enzymology to discover function: BioH, a new carboxylesterase from Escherichia coli. J Biol Chem (2003) 1.61
Data mining crystallization databases: knowledge-based approaches to optimize protein crystal screens. Proteins (2003) 1.58
Staphylococcus aureus IsdG and IsdI, heme-degrading enzymes with structural similarity to monooxygenases. J Biol Chem (2004) 1.58
Large-scale evaluation of protein reductive methylation for improving protein crystallization. Nat Methods (2008) 1.55
Quantum model of catalysis based on a mobile proton revealed by subatomic x-ray and neutron diffraction studies of h-aldose reductase. Proc Natl Acad Sci U S A (2008) 1.55
Contributions to the NIH-NIGMS Protein Structure Initiative from the PSI Production Centers. Structure (2008) 1.52
General enzymatic screens identify three new nucleotidases in Escherichia coli. Biochemical characterization of SurE, YfbR, and YjjG. J Biol Chem (2004) 1.50
B. subtilis ykuD protein at 2.0 A resolution: insights into the structure and function of a novel, ubiquitous family of bacterial enzymes. Proteins (2006) 1.47
Structural plasticity in the oestrogen receptor ligand-binding domain. EMBO Rep (2007) 1.47
Time-controlled microfluidic seeding in nL-volume droplets to separate nucleation and growth stages of protein crystallization. Angew Chem Int Ed Engl (2006) 1.45
High-throughput protein purification and quality assessment for crystallization. Methods (2011) 1.44
Riboneogenesis in yeast. Cell (2011) 1.43
Crystal structure of Enterococcus faecalis SlyA-like transcriptional factor. J Biol Chem (2003) 1.42
Structure- and function-based characterization of a new phosphoglycolate phosphatase from Thermoplasma acidophilum. J Biol Chem (2003) 1.41
Crystal structure of murine sCEACAM1a[1,4]: a coronavirus receptor in the CEA family. EMBO J (2002) 1.37
Methyltransferase that modifies guanine 966 of the 16 S rRNA: functional identification and tertiary structure. J Biol Chem (2006) 1.33
Elemental isomerism: a boron-nitrogen surrogate for a carbon-carbon double bond increases the chemical diversity of estrogen receptor ligands. Chem Biol (2007) 1.31
Strategies for structural proteomics of prokaryotes: Quantifying the advantages of studying orthologous proteins and of using both NMR and X-ray crystallography approaches. Proteins (2003) 1.27
Towards fully automated structure-based function prediction in structural genomics: a case study. J Mol Biol (2007) 1.26
The crystal structure of a TL/CD8alphaalpha complex at 2.1 A resolution: implications for modulation of T cell activation and memory. Immunity (2003) 1.25
The structures of the thrombospondin-1 N-terminal domain and its complex with a synthetic pentameric heparin. Structure (2006) 1.25
Structural basis for dimerization of ICAM-1 on the cell surface. Mol Cell (2004) 1.24
High-throughput production of prokaryotic membrane proteins. J Struct Funct Genomics (2005) 1.24
Glyoxylate and pyruvate are antagonistic effectors of the Escherichia coli IclR transcriptional regulator. J Biol Chem (2007) 1.22
Structure of the retinal determination protein Dachshund reveals a DNA binding motif. Structure (2002) 1.22
Molecular basis of formaldehyde detoxification. Characterization of two S-formylglutathione hydrolases from Escherichia coli, FrmB and YeiG. J Biol Chem (2006) 1.22
A comprehensive analysis of structural and sequence conservation in the TetR family transcriptional regulators. J Mol Biol (2010) 1.21