Published in Nat Methods on March 02, 2014
CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes. Protein Cell (2015) 7.80
Passenger Mutations Confound Interpretation of All Genetically Modified Congenic Mice. Immunity (2015) 2.60
Somatic mosaicism and allele complexity induced by CRISPR/Cas9 RNA injections in mouse zygotes. Dev Biol (2014) 1.96
Gene disruption by cell-penetrating peptide-mediated delivery of Cas9 protein and guide RNA. Genome Res (2014) 1.91
Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system. Sci Rep (2014) 1.83
A mouse geneticist's practical guide to CRISPR applications. Genetics (2014) 1.81
High-throughput genome editing and phenotyping facilitated by high resolution melting curve analysis. PLoS One (2014) 1.60
The Fanconi Anemia Pathway Maintains Genome Stability by Coordinating Replication and Transcription. Mol Cell (2015) 1.59
Retracted Robust method for TALEN-edited correction of pF508del in patient-specific induced pluripotent stem cells. Stem Cell Res Ther (2016) 1.43
CRISPR/Cas9-mediated conversion of human platelet alloantigen allotypes. Blood (2015) 1.42
A CRISPR view of development. Genes Dev (2014) 1.41
Protospacer adjacent motif (PAM)-distal sequences engage CRISPR Cas9 DNA target cleavage. PLoS One (2014) 1.38
Optimization of genome engineering approaches with the CRISPR/Cas9 system. PLoS One (2014) 1.27
Targeting non-coding RNAs with the CRISPR/Cas9 system in human cell lines. Nucleic Acids Res (2014) 1.26
Efficient and allele-specific genome editing of disease loci in human iPSCs. Mol Ther (2014) 1.25
Off-target Effects in CRISPR/Cas9-mediated Genome Engineering. Mol Ther Nucleic Acids (2015) 1.23
Off-target mutations are rare in Cas9-modified mice. Nat Methods (2015) 1.14
Dual sgRNA-directed gene knockout using CRISPR/Cas9 technology in Caenorhabditis elegans. Sci Rep (2014) 1.12
Advances in genome editing technology and its promising application in evolutionary and ecological studies. Gigascience (2014) 1.11
Advances in CRISPR-Cas9 genome engineering: lessons learned from RNA interference. Nucleic Acids Res (2015) 1.10
Highly efficient targeted mutagenesis in one-cell mouse embryos mediated by the TALEN and CRISPR/Cas systems. Sci Rep (2014) 1.10
Efficient inversions and duplications of mammalian regulatory DNA elements and gene clusters by CRISPR/Cas9. J Mol Cell Biol (2015) 1.07
Efficient generation of gene-modified pigs via injection of zygote with Cas9/sgRNA. Sci Rep (2015) 1.07
Induction of targeted, heritable mutations in barley and Brassica oleracea using RNA-guided Cas9 nuclease. Genome Biol (2015) 1.06
Development of an intein-mediated split-Cas9 system for gene therapy. Nucleic Acids Res (2015) 1.03
A CRISPR/Cas9 Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulation. Plant Physiol (2015) 1.03
Both TALENs and CRISPR/Cas9 directly target the HBB IVS2-654 (C > T) mutation in β-thalassemia-derived iPSCs. Sci Rep (2015) 1.03
Conditional targeting of Ispd using paired Cas9 nickase and a single DNA template in mice. FEBS Open Bio (2014) 1.01
Large genomic fragment deletions and insertions in mouse using CRISPR/Cas9. PLoS One (2015) 1.01
Rapid and efficient one-step generation of paired gRNA CRISPR-Cas9 libraries. Nat Commun (2015) 1.00
Cellular mechanosensing: getting to the nucleus of it all. Prog Biophys Mol Biol (2014) 0.99
Creating and evaluating accurate CRISPR-Cas9 scalpels for genomic surgery. Nat Methods (2015) 0.96
CRISPR/Cas9-induced knockout and knock-in mutations in Chlamydomonas reinhardtii. Sci Rep (2016) 0.94
CRISPR-Cas9 mediated efficient PD-1 disruption on human primary T cells from cancer patients. Sci Rep (2016) 0.93
The generation of kidney organoids by differentiation of human pluripotent cells to ureteric bud progenitor-like cells. Nat Protoc (2014) 0.93
Large genomic fragment deletion and functional gene cassette knock-in via Cas9 protein mediated genome editing in one-cell rodent embryos. Sci Rep (2015) 0.93
A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell (2017) 0.92
GONAD: Genome-editing via Oviductal Nucleic Acids Delivery system: a novel microinjection independent genome engineering method in mice. Sci Rep (2015) 0.92
CRISPR/Cas9-mediated gene knockout in the ascidian Ciona intestinalis. Dev Growth Differ (2014) 0.91
Concise review: Generation of neurons from somatic cells of healthy individuals and neurological patients through induced pluripotency or direct conversion. Stem Cells (2014) 0.91
Methods for Optimizing CRISPR-Cas9 Genome Editing Specificity. Mol Cell (2016) 0.91
Production of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes. BMC Biotechnol (2015) 0.90
Insertion of sequences at the original provirus integration site of mouse ROSA26 locus using the CRISPR/Cas9 system. FEBS Open Bio (2015) 0.90
Detailed phenotypic and molecular analyses of genetically modified mice generated by CRISPR-Cas9-mediated editing. PLoS One (2015) 0.89
WGE: a CRISPR database for genome engineering. Bioinformatics (2015) 0.89
Genomic editing tools to model human diseases with isogenic pluripotent stem cells. Stem Cells Dev (2014) 0.89
EXD2 promotes homologous recombination by facilitating DNA end resection. Nat Cell Biol (2016) 0.88
Conserved higher-order chromatin regulates NMDA receptor gene expression and cognition. Neuron (2014) 0.88
CRISPR/Cas9 nickase-mediated disruption of hepatitis B virus open reading frame S and X. Sci Rep (2015) 0.87
Approaches for establishing the function of regulatory genetic variants involved in disease. Genome Med (2014) 0.87
Creation of targeted genomic deletions using TALEN or CRISPR/Cas nuclease pairs in one-cell mouse embryos. FEBS Open Bio (2014) 0.87
Genome editing strategies: potential tools for eradicating HIV-1/AIDS. J Neurovirol (2015) 0.87
Highly efficient CRISPR/Cas9-mediated transgene knockin at the H11 locus in pigs. Sci Rep (2015) 0.86
Functional genetics for all: engineered nucleases, CRISPR and the gene editing revolution. Evodevo (2014) 0.86
The CRISPR/Cas9 genome editing methodology as a weapon against human viruses. Discov Med (2015) 0.86
Correction of the auditory phenotype in C57BL/6N mice via CRISPR/Cas9-mediated homology directed repair. Genome Med (2016) 0.86
CRISPR-based self-cleaving mechanism for controllable gene delivery in human cells. Nucleic Acids Res (2014) 0.86
Genome editing in cardiovascular diseases. Nat Rev Cardiol (2016) 0.86
Efficient fdCas9 Synthetic Endonuclease with Improved Specificity for Precise Genome Engineering. PLoS One (2015) 0.85
CRISPR/Cas9-based generation of knockdown mice by intronic insertion of artificial microRNA using longer single-stranded DNA. Sci Rep (2015) 0.85
Efficient Genome Editing in Chicken DF-1 Cells Using the CRISPR/Cas9 System. G3 (Bethesda) (2016) 0.85
CRISPR-Cas9-mediated genome editing and guide RNA design. Mamm Genome (2015) 0.84
Streptococcus thermophilus CRISPR-Cas9 Systems Enable Specific Editing of the Human Genome. Mol Ther (2015) 0.84
Mouse Genome Editing Using the CRISPR/Cas System. Curr Protoc Hum Genet (2014) 0.84
CRISPR-Cas9 as a Powerful Tool for Efficient Creation of Oncolytic Viruses. Viruses (2016) 0.83
CRISPR-Cas9-based knockout of the prion protein and its effect on the proteome. PLoS One (2014) 0.83
The therapeutic application of CRISPR/Cas9 technologies for HIV. Expert Opin Biol Ther (2015) 0.83
CRISPR/Cas9-mediated reporter knock-in in mouse haploid embryonic stem cells. Sci Rep (2015) 0.83
Reassessment of the Four Yield-related Genes Gn1a, DEP1, GS3, and IPA1 in Rice Using a CRISPR/Cas9 System. Front Plant Sci (2016) 0.83
Gene correction in patient-specific iPSCs for therapy development and disease modeling. Hum Genet (2016) 0.83
Molecular substrates of schizophrenia: homeostatic signaling to connectivity. Mol Psychiatry (2015) 0.82
Real-time observation of DNA recognition and rejection by the RNA-guided endonuclease Cas9. Nat Commun (2016) 0.81
CRISPR/Cas9 and cancer targets: future possibilities and present challenges. Oncotarget (2016) 0.81
Future of breeding by genome editing is in the hands of regulators. GM Crops Food (2015) 0.81
An alternative strategy for targeted gene replacement in plants using a dual-sgRNA/Cas9 design. Sci Rep (2016) 0.80
Combining CRISPR/Cas9 and rAAV Templates for Efficient Gene Editing. Nucleic Acid Ther (2015) 0.79
Correction of Monogenic and Common Retinal Disorders with Gene Therapy. Genes (Basel) (2017) 0.79
CRISPR-Cas9(D10A) nickase-based genotypic and phenotypic screening to enhance genome editing. Sci Rep (2016) 0.78
Utilization of TALEN and CRISPR/Cas9 technologies for gene targeting and modification. Exp Biol Med (Maywood) (2015) 0.78
Targeting cellular mRNAs translation by CRISPR-Cas9. Sci Rep (2016) 0.78
CRISPR/Cas9-Mediated Correction of the Sickle Mutation in Human CD34+ cells. Mol Ther (2016) 0.78
Genome Editing and Its Applications in Model Organisms. Genomics Proteomics Bioinformatics (2016) 0.77
Precision Targeted Mutagenesis via Cas9 Paired Nickases in Rice. Plant Cell Physiol (2016) 0.77
PCR-Based Seamless Genome Editing with High Efficiency and Fidelity in Escherichia coli. PLoS One (2016) 0.77
Minimizing off-Target Mutagenesis Risks Caused by Programmable Nucleases. Int J Mol Sci (2015) 0.77
Genome engineering: Drosophila melanogaster and beyond. Wiley Interdiscip Rev Dev Biol (2015) 0.77
Exome sequencing in the knockin mice generated using the CRISPR/Cas system. Sci Rep (2016) 0.77
New approaches to manipulating the epigenome. Dialogues Clin Neurosci (2014) 0.77
CRISPR/Cas9-mediated knockout of factors in non-homologous end joining pathway enhances gene targeting in silkworm cells. Sci Rep (2015) 0.77
TALENs Facilitate Single-step Seamless SDF Correction of F508del CFTR in Airway Epithelial Submucosal Gland Cell-derived CF-iPSCs. Mol Ther Nucleic Acids (2016) 0.77
A genome editing primer for the hematologist. Blood (2016) 0.77
Efficient CRISPR-rAAV engineering of endogenous genes to study protein function by allele-specific RNAi. Nucleic Acids Res (2015) 0.77
Generation and Characterization of a MYF5 Reporter Human iPS Cell Line Using CRISPR/Cas9 Mediated Homologous Recombination. Sci Rep (2016) 0.77
Focused screening of mitochondrial metabolism reveals a crucial role for a tumor suppressor Hbp1 in ovarian reserve. Cell Death Differ (2016) 0.77
Rapid prototyping of microbial cell factories via genome-scale engineering. Biotechnol Adv (2014) 0.76
CRISPR-Cas9 enables conditional mutagenesis of challenging loci. Sci Rep (2016) 0.76
CRISPR/Cas9 Targeted Gene Editing and Cellular Engineering in Fanconi Anemia. Stem Cells Dev (2016) 0.76
Generation of an Oocyte-Specific Cas9 Transgenic Mouse for Genome Editing. PLoS One (2016) 0.76
Reversible Cysteine Acylation Regulates the Activity of Human Palmitoyl-Protein Thioesterase 1 (PPT1). PLoS One (2016) 0.76
Editing the Mouse Genome Using the CRISPR-Cas9 System. Cold Spring Harb Protoc (2016) 0.76
Off-target mutations are rare in Cas9-modified mice. Nat Methods (2015) 1.14
CRISPR-Cas9 enables conditional mutagenesis of challenging loci. Sci Rep (2016) 0.76