Published in Mamm Genome on May 20, 2015
An Overview of CRISPR-Based Tools and Their Improvements: New Opportunities in Understanding Plant-Pathogen Interactions for Better Crop Protection. Front Plant Sci (2016) 0.80
The ever-expanding myokinome: discovery challenges and therapeutic implications. Nat Rev Drug Discov (2016) 0.80
Comparison of CRISPR/Cas9 and TALENs on editing an integrated EGFP gene in the genome of HEK293FT cells. Springerplus (2016) 0.77
Generating Mouse Models Using CRISPR-Cas9-Mediated Genome Editing. Curr Protoc Mouse Biol (2016) 0.76
Generation of genetically modified mice using CRISPR/Cas9 and haploid embryonic stem cell systems. Dongwuxue Yanjiu (2016) 0.75
Efficient identification of CRISPR/Cas9-induced insertions/deletions by direct germline screening in zebrafish. BMC Genomics (2016) 0.75
Genome Editing and Muscle Stem Cells as a Therapeutic Tool for Muscular Dystrophies. Curr Stem Cell Rep (2017) 0.75
An integrated encyclopedia of DNA elements in the human genome. Nature (2012) 64.73
Multiplex genome engineering using CRISPR/Cas systems. Science (2013) 55.53
RNA-guided human genome engineering via Cas9. Science (2013) 48.29
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science (2012) 48.17
One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering. Cell (2013) 24.00
Breaking the code of DNA binding specificity of TAL-type III effectors. Science (2009) 22.33
Efficient genome editing in zebrafish using a CRISPR-Cas system. Nat Biotechnol (2013) 21.32
A simple cipher governs DNA recognition by TAL effectors. Science (2009) 19.99
DNA targeting specificity of RNA-guided Cas9 nucleases. Nat Biotechnol (2013) 19.99
Highly efficient endogenous human gene correction using designed zinc-finger nucleases. Nature (2005) 19.15
Genome editing with engineered zinc finger nucleases. Nat Rev Genet (2010) 18.88
CRISPR/Cas, the immune system of bacteria and archaea. Science (2010) 18.72
High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells. Nat Biotechnol (2013) 18.01
CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III. Nature (2011) 17.38
RNA-programmed genome editing in human cells. Elife (2013) 14.82
Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease. Nat Biotechnol (2013) 14.28
Development and applications of CRISPR-Cas9 for genome engineering. Cell (2014) 13.50
Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity. Cell (2013) 13.37
Genome-scale CRISPR-Cas9 knockout screening in human cells. Science (2013) 12.36
CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering. Nat Biotechnol (2013) 11.35
RNA-guided RNA cleavage by a CRISPR RNA-Cas protein complex. Cell (2009) 11.12
Genetic screens in human cells using the CRISPR-Cas9 system. Science (2013) 10.75
Genome editing. The new frontier of genome engineering with CRISPR-Cas9. Science (2014) 10.43
CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes. Cell (2013) 9.55
TAL effectors: customizable proteins for DNA targeting. Science (2011) 9.47
One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated genome engineering. Cell (2013) 7.99
Improving CRISPR-Cas nuclease specificity using truncated guide RNAs. Nat Biotechnol (2014) 6.85
Generation of gene-modified cynomolgus monkey via Cas9/RNA-mediated gene targeting in one-cell embryos. Cell (2014) 6.11
Self versus non-self discrimination during CRISPR RNA-directed immunity. Nature (2010) 5.89
Genome editing with Cas9 in adult mice corrects a disease mutation and phenotype. Nat Biotechnol (2014) 5.71
GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases. Nat Biotechnol (2014) 5.69
Dynamic imaging of genomic loci in living human cells by an optimized CRISPR/Cas system. Cell (2013) 5.40
Nucleotide sequence of the iap gene, responsible for alkaline phosphatase isozyme conversion in Escherichia coli, and identification of the gene product. J Bacteriol (1987) 5.28
Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Nature (2014) 5.19
Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells. Nat Biotechnol (2014) 5.07
Sequence features and chromatin structure around the genomic regions bound by 119 human transcription factors. Genome Res (2012) 4.80
Orthogonal Cas9 proteins for RNA-guided gene regulation and editing. Nat Methods (2013) 4.61
Heritable gene targeting in the mouse and rat using a CRISPR-Cas system. Nat Biotechnol (2013) 4.58
Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing. Nat Biotechnol (2014) 4.41
Genome-wide analysis reveals characteristics of off-target sites bound by the Cas9 endonuclease. Nat Biotechnol (2014) 4.16
Essential features and rational design of CRISPR RNAs that function with the Cas RAMP module complex to cleave RNAs. Mol Cell (2012) 3.96
Engineering complex synthetic transcriptional programs with CRISPR RNA scaffolds. Cell (2014) 3.76
Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification. Nat Biotechnol (2014) 3.72
Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation. Nat Biotechnol (2014) 3.66
Simultaneous generation and germline transmission of multiple gene mutations in rat using CRISPR-Cas systems. Nat Biotechnol (2013) 3.65
Ubiquitous heterogeneity and asymmetry of the chromatin environment at regulatory elements. Genome Res (2012) 3.61
ZiFiT (Zinc Finger Targeter): an updated zinc finger engineering tool. Nucleic Acids Res (2010) 3.59
Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis. Proc Natl Acad Sci U S A (2013) 3.53
Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. Cell Res (2013) 3.50
Zinc Finger Targeter (ZiFiT): an engineered zinc finger/target site design tool. Nucleic Acids Res (2007) 3.47
Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects. Nat Methods (2014) 3.34
CRISPR-mediated direct mutation of cancer genes in the mouse liver. Nature (2014) 3.28
Genome-wide detection of DNA double-stranded breaks induced by engineered nucleases. Nat Biotechnol (2014) 2.99
Optimization of scarless human stem cell genome editing. Nucleic Acids Res (2013) 2.78
E-CRISP: fast CRISPR target site identification. Nat Methods (2014) 2.73
CRISPR/Cas9 systems have off-target activity with insertions or deletions between target DNA and guide RNA sequences. Nucleic Acids Res (2014) 2.59
Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases. Bioinformatics (2014) 2.54
In vivo interrogation of gene function in the mammalian brain using CRISPR-Cas9. Nat Biotechnol (2014) 2.51
CHOPCHOP: a CRISPR/Cas9 and TALEN web tool for genome editing. Nucleic Acids Res (2014) 2.36
Programmable RNA recognition and cleavage by CRISPR/Cas9. Nature (2014) 2.33
Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells. Nat Methods (2015) 2.29
One-step generation of knockout pigs by zygote injection of CRISPR/Cas system. Cell Res (2014) 2.20
Characterization of genomic deletion efficiency mediated by clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 nuclease system in mammalian cells. J Biol Chem (2014) 2.18
Unbiased detection of off-target cleavage by CRISPR-Cas9 and TALENs using integrase-defective lentiviral vectors. Nat Biotechnol (2015) 2.03
Microhomology-based choice of Cas9 nuclease target sites. Nat Methods (2014) 1.82
Memory of viral infections by CRISPR-Cas adaptive immune systems: acquisition of new information. Virology (2012) 1.80
Meganucleases and other tools for targeted genome engineering: perspectives and challenges for gene therapy. Curr Gene Ther (2011) 1.76
Targeted genomic rearrangements using CRISPR/Cas technology. Nat Commun (2014) 1.70
Correction of a genetic disease by CRISPR-Cas9-mediated gene editing in mouse spermatogonial stem cells. Cell Res (2014) 1.45
Complete humanization of the mouse immunoglobulin loci enables efficient therapeutic antibody discovery. Nat Biotechnol (2014) 1.37
Simple and rapid in vivo generation of chromosomal rearrangements using CRISPR/Cas9 technology. Cell Rep (2014) 1.30
Multi-kilobase homozygous targeted gene replacement in human induced pluripotent stem cells. Nucleic Acids Res (2014) 1.26
Highly efficient targeted chromosome deletions using CRISPR/Cas9. Biotechnol Bioeng (2014) 0.95