Published in Development on November 01, 2014
Rapid reverse genetic screening using CRISPR in zebrafish. Nat Methods (2015) 2.04
Genome engineering with CRISPR-Cas9 in the mosquito Aedes aegypti. Cell Rep (2015) 1.28
ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes. Nat Commun (2016) 0.99
Metabolic insights from zebrafish genetics, physiology, and chemical biology. Cell Mol Life Sci (2015) 0.88
FusX: A Rapid One-Step Transcription Activator-Like Effector Assembly System for Genome Science. Hum Gene Ther (2016) 0.88
Xenopus pax6 mutants affect eye development and other organ systems, and have phenotypic similarities to human aniridia patients. Dev Biol (2015) 0.87
The renaissance of developmental biology. PLoS Biol (2015) 0.86
CRISPR/Cas9-induced disruption of gene expression in mouse embryonic brain and single neural stem cells in vivo. EMBO Rep (2016) 0.83
Origins of Programmable Nucleases for Genome Engineering. J Mol Biol (2015) 0.83
ssDNA and the Argonautes: The Quest for the Next Golden Editor. Hum Gene Ther (2016) 0.82
TALEN-mediated apc mutation in Xenopus tropicalis phenocopies familial adenomatous polyposis. Oncoscience (2015) 0.81
Strategies for precision modulation of gene expression by epigenome editing: an overview. Epigenetics Chromatin (2015) 0.79
Comparative assessment of fluorescent proteins for in vivo imaging in an animal model system. Mol Biol Cell (2016) 0.79
Targeted mutagenesis of aryl hydrocarbon receptor 2a and 2b genes in Atlantic killifish (Fundulus heteroclitus). Aquat Toxicol (2014) 0.78
Expanding the genetic toolkit in Xenopus: Approaches and opportunities for human disease modeling. Dev Biol (2016) 0.77
Genome editing: the road of CRISPR/Cas9 from bench to clinic. Exp Mol Med (2016) 0.77
Mayo Clinic Zebrafish Facility Overview. Zebrafish (2016) 0.77
A quick guide to CRISPR sgRNA design tools. GM Crops Food (2015) 0.77
Manipulating the Gut Microbiota: Methods and Challenges. ILAR J (2015) 0.77
Generation of a Xenopus laevis F1 albino J strain by genome editing and oocyte host-transfer. Dev Biol (2016) 0.77
Genome engineering: Drosophila melanogaster and beyond. Wiley Interdiscip Rev Dev Biol (2015) 0.77
Xenopus as a Model for GI/Pancreas Disease. Curr Pathobiol Rep (2015) 0.77
CRISPR applications in ophthalmologic genome surgery. Curr Opin Ophthalmol (2017) 0.76
TALEN mediated targeted editing of GM2/GD2-synthase gene modulates anchorage independent growth by reducing anoikis resistance in mouse tumor cells. Sci Rep (2015) 0.76
Biallelic editing of a lamprey genome using the CRISPR/Cas9 system. Sci Rep (2016) 0.75
T cell-specific inactivation of mouse CD2 by CRISPR/Cas9. Sci Rep (2016) 0.75
A Novel Zebrafish ret Heterozygous Model of Hirschsprung Disease Identifies a Functional Role for mapk10 as a Modifier of Enteric Nervous System Phenotype Severity. PLoS Genet (2016) 0.75
An optimized TALEN application for mutagenesis and screening in Drosophila melanogaster. Cell Logist (2015) 0.75
Balancing copy number in ribosomal DNA. Proc Natl Acad Sci U S A (2015) 0.75
Allele-Specific Quantitative PCR for Accurate, Rapid, and Cost-Effective Genotyping. Hum Gene Ther (2016) 0.75
Gene Tagging Strategies To Assess Protein Expression, Localization, and Function in Drosophila. Genetics (2017) 0.75
Studying disorders of vertebrate iron and heme metabolism using zebrafish. Methods Cell Biol (2016) 0.75
An efficient system for homology-dependent targeted gene integration in medaka (Oryzias latipes). Zoological Lett (2017) 0.75
Multiplex genome engineering using CRISPR/Cas systems. Science (2013) 55.53
RNA-guided human genome engineering via Cas9. Science (2013) 48.29
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science (2012) 48.17
CRISPR provides acquired resistance against viruses in prokaryotes. Science (2007) 29.74
One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering. Cell (2013) 24.00
Breaking the code of DNA binding specificity of TAL-type III effectors. Science (2009) 22.33
Efficient genome editing in zebrafish using a CRISPR-Cas system. Nat Biotechnol (2013) 21.32
A simple cipher governs DNA recognition by TAL effectors. Science (2009) 19.99
Highly efficient endogenous human gene correction using designed zinc-finger nucleases. Nature (2005) 19.15
CRISPR/Cas, the immune system of bacteria and archaea. Science (2010) 18.72
Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Nucleic Acids Res (2011) 16.53
Genome engineering using the CRISPR-Cas9 system. Nat Protoc (2013) 15.98
RNA-programmed genome editing in human cells. Elife (2013) 14.82
Targeting DNA double-strand breaks with TAL effector nucleases. Genetics (2010) 14.49
Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression. Cell (2013) 13.43
Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity. Cell (2013) 13.37
FLASH assembly of TALENs for high-throughput genome editing. Nat Biotechnol (2012) 12.97
Knockout rats via embryo microinjection of zinc-finger nucleases. Science (2009) 12.33
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Chimeric nucleases stimulate gene targeting in human cells. Science (2003) 12.12
Genetic engineering of human pluripotent cells using TALE nucleases. Nat Biotechnol (2011) 11.76
A putative RNA-interference-based immune system in prokaryotes: computational analysis of the predicted enzymatic machinery, functional analogies with eukaryotic RNAi, and hypothetical mechanisms of action. Biol Direct (2006) 11.31
Short motif sequences determine the targets of the prokaryotic CRISPR defence system. Microbiology (2009) 10.67
Targeted genome editing across species using ZFNs and TALENs. Science (2011) 9.84
CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes. Cell (2013) 9.55
TAL effectors: customizable proteins for DNA targeting. Science (2011) 9.47
Targeted chromosomal cleavage and mutagenesis in Drosophila using zinc-finger nucleases. Genetics (2002) 9.09
Selection-free zinc-finger-nuclease engineering by context-dependent assembly (CoDA). Nat Methods (2010) 8.86
Gene targeting by homologous recombination in Drosophila. Science (2000) 8.63
Knockout rats generated by embryo microinjection of TALENs. Nat Biotechnol (2011) 8.45
Enhancing gene targeting with designed zinc finger nucleases. Science (2003) 8.42
High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity. Nat Biotechnol (2013) 8.40
A novel TALE nuclease scaffold enables high genome editing activity in combination with low toxicity. Nucleic Acids Res (2011) 7.93
Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems. Nucleic Acids Res (2013) 7.77
In vivo genome editing using a high-efficiency TALEN system. Nature (2012) 7.11
Revealing off-target cleavage specificities of zinc-finger nucleases by in vitro selection. Nat Methods (2011) 6.90
Cas9 as a versatile tool for engineering biology. Nat Methods (2013) 6.87
Improving CRISPR-Cas nuclease specificity using truncated guide RNAs. Nat Biotechnol (2014) 6.85
Stimulation of homologous recombination through targeted cleavage by chimeric nucleases. Mol Cell Biol (2001) 6.68
TAL nucleases (TALNs): hybrid proteins composed of TAL effectors and FokI DNA-cleavage domain. Nucleic Acids Res (2010) 6.65
Unexpected failure rates for modular assembly of engineered zinc fingers. Nat Methods (2008) 6.51
An unbiased genome-wide analysis of zinc-finger nuclease specificity. Nat Biotechnol (2011) 6.44
Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases. Genome Res (2013) 6.19
De novo-engineered transcription activator-like effector (TALE) hybrid nuclease with novel DNA binding specificity creates double-strand breaks. Proc Natl Acad Sci U S A (2011) 6.12
Generation of gene-modified cynomolgus monkey via Cas9/RNA-mediated gene targeting in one-cell embryos. Cell (2014) 6.11
Enhancing zinc-finger-nuclease activity with improved obligate heterodimeric architectures. Nat Methods (2010) 6.00
DNA interrogation by the CRISPR RNA-guided endonuclease Cas9. Nature (2014) 5.70
High-frequency genome editing using ssDNA oligonucleotides with zinc-finger nucleases. Nat Methods (2011) 5.68
Targeted integration in rat and mouse embryos with zinc-finger nucleases. Nat Biotechnol (2010) 5.60
Toward controlling gene expression at will: specific regulation of the erbB-2/HER-2 promoter by using polydactyl zinc finger proteins constructed from modular building blocks. Proc Natl Acad Sci U S A (1998) 5.46
Crystal structure of Cas9 in complex with guide RNA and target DNA. Cell (2014) 5.26
Structures of Cas9 endonucleases reveal RNA-mediated conformational activation. Science (2014) 5.13
Efficient TALEN-mediated gene knockout in livestock. Proc Natl Acad Sci U S A (2012) 4.99
Modularly assembled designer TAL effector nucleases for targeted gene knockout and gene replacement in eukaryotes. Nucleic Acids Res (2011) 4.75
CRISPR RNA-guided activation of endogenous human genes. Nat Methods (2013) 4.69
Genome engineering with zinc-finger nucleases. Genetics (2011) 4.66
Orthogonal Cas9 proteins for RNA-guided gene regulation and editing. Nat Methods (2013) 4.61
Efficient gene targeting in Drosophila with zinc-finger nucleases. Genetics (2006) 4.47
Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing. Nat Biotechnol (2014) 4.41
Genome-wide analysis reveals characteristics of off-target sites bound by the Cas9 endonuclease. Nat Biotechnol (2014) 4.16
CRISPR/Cas9 systems targeting β-globin and CCR5 genes have substantial off-target activity. Nucleic Acids Res (2013) 4.11
DNA-binding specificity is a major determinant of the activity and toxicity of zinc-finger nucleases. Mol Ther (2007) 3.94
Locus-specific editing of histone modifications at endogenous enhancers. Nat Biotechnol (2013) 3.82
Genome editing with modularly assembled zinc-finger nucleases. Nat Methods (2010) 3.79
Mutation detection using Surveyor nuclease. Biotechniques (2004) 3.72
Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification. Nat Biotechnol (2014) 3.72
Efficient gene targeting in Drosophila by direct embryo injection with zinc-finger nucleases. Proc Natl Acad Sci U S A (2008) 3.70
Comprehensive interrogation of natural TALE DNA-binding modules and transcriptional repressor domains. Nat Commun (2012) 3.60
Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. Cell Res (2013) 3.50
Highly specific and efficient CRISPR/Cas9-catalyzed homology-directed repair in Drosophila. Genetics (2014) 3.07
Precision genome engineering with programmable DNA-nicking enzymes. Genome Res (2012) 3.05
Expanding or restricting the target site repertoire of zinc-finger nucleases: the inter-domain linker as a major determinant of target site selectivity. Mol Ther (2008) 3.02
Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis, tobacco, sorghum and rice. Nucleic Acids Res (2013) 2.99
High-resolution DNA melting analysis: advancements and limitations. Hum Mutat (2009) 2.90
Targeted DNA demethylation and activation of endogenous genes using programmable TALE-TET1 fusion proteins. Nat Biotechnol (2013) 2.89
Optimization of scarless human stem cell genome editing. Nucleic Acids Res (2013) 2.78
Chromosomal translocations induced at specified loci in human stem cells. Proc Natl Acad Sci U S A (2009) 2.73
Chromosomal deletions and inversions mediated by TALENs and CRISPR/Cas in zebrafish. Nucleic Acids Res (2013) 2.73
Manipulating the mammalian genome by homologous recombination. Proc Natl Acad Sci U S A (2001) 2.71
TALEN-mediated precise genome modification by homologous recombination in zebrafish. Nat Methods (2013) 2.64
Direct production of mouse disease models by embryo microinjection of TALENs and oligodeoxynucleotides. Proc Natl Acad Sci U S A (2013) 2.63
Zinc-finger nuclease-driven targeted integration into mammalian genomes using donors with limited chromosomal homology. Nucleic Acids Res (2010) 2.61
Highly efficient CRISPR/Cas9-mediated knock-in in zebrafish by homology-independent DNA repair. Genome Res (2013) 2.60
Highly improved gene targeting by germline-specific Cas9 expression in Drosophila. Genetics (2013) 2.50
The tracrRNA and Cas9 families of type II CRISPR-Cas immunity systems. RNA Biol (2013) 2.44
Transcription activator-like effector nucleases enable efficient plant genome engineering. Plant Physiol (2012) 2.36
Phylogeny of Cas9 determines functional exchangeability of dual-RNA and Cas9 among orthologous type II CRISPR-Cas systems. Nucleic Acids Res (2013) 2.35
Adeno-associated virus vectors integrate at chromosome breakage sites. Nat Genet (2004) 2.27
RNA-guided genome editing in plants using a CRISPR-Cas system. Mol Plant (2013) 2.25
Efficient nonmeiotic allele introgression in livestock using custom endonucleases. Proc Natl Acad Sci U S A (2013) 2.22
Optimized gene editing technology for Drosophila melanogaster using germ line-specific Cas9. Proc Natl Acad Sci U S A (2013) 2.13
Obligate ligation-gated recombination (ObLiGaRe): custom-designed nuclease-mediated targeted integration through nonhomologous end joining. Genome Res (2012) 2.12
A ligation-independent cloning technique for high-throughput assembly of transcription activator–like effector genes. Nat Biotechnol (2013) 1.98
Donor DNA Utilization During Gene Targeting with Zinc-Finger Nucleases. G3 (Bethesda) (2013) 1.92
Autonomous zinc-finger nuclease pairs for targeted chromosomal deletion. Nucleic Acids Res (2010) 1.92
TALEN-based gene correction for epidermolysis bullosa. Mol Ther (2013) 1.84
An iCRISPR platform for rapid, multiplexable, and inducible genome editing in human pluripotent stem cells. Cell Stem Cell (2014) 1.83
Efficient generation of large-scale genome-modified mice using gRNA and CAS9 endonuclease. Nucleic Acids Res (2013) 1.83
Zinc finger protein-dependent and -independent contributions to the in vivo off-target activity of zinc finger nucleases. Nucleic Acids Res (2010) 1.81
Gene targeting in Drosophila and Caenorhabditis elegans with zinc-finger nucleases. Methods Mol Biol (2008) 1.81