Computational image analysis of colony and nuclear morphology to evaluate human induced pluripotent stem cells.

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🔗 View Article (PMC 4227026)

Published in Sci Rep on November 11, 2014

Authors

Kazuaki Tokunaga1, Noriko Saitoh1, Ilya G Goldberg2, Chiyomi Sakamoto3, Yoko Yasuda3, Yoshinori Yoshida4, Shinya Yamanaka5, Mitsuyoshi Nakao1

Author Affiliations

1: 1] Department of Medical Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto University, 2-2-1 Honjo, Chuo-ku, Kumamoto 860-0811, Japan [2] Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency, Tokyo, Japan.
2: Image Informatics and Computational Biology Unit, Laboratory of Genetics, National Institute on Aging, National Institutes of Health, 251 Bayview Boulevard, Suite 100, Baltimore, MD 21224, USA.
3: Department of Medical Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto University, 2-2-1 Honjo, Chuo-ku, Kumamoto 860-0811, Japan.
4: Center for iPS Cell Research and Application, Kyoto University, 53 Shogoin-Kawaharacho, Sakyo-ku, Kyoto, 606-8507, Japan.
5: 1] Center for iPS Cell Research and Application, Kyoto University, 53 Shogoin-Kawaharacho, Sakyo-ku, Kyoto, 606-8507, Japan [2] Gladstone Institute of Cardiovascular Disease, 1650 Owens Street, San Francisco, CA 94158, USA.

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