Efficient cellular solid-state NMR of membrane proteins by targeted protein labeling.

(1) H-Detected Solid-State NMR Studies of Water-Inaccessible Proteins In Vitro and In Situ. Angew Chem Int Ed Engl (2016) 0.83

Probing Conformational Changes during the Gating Cycle of a Potassium Channel in Lipid Bilayers. Biophys J (2017) 0.76

Structural mechanism for rifampicin inhibition of bacterial rna polymerase. Cell (2001) 6.10

An improved broadband decoupling sequence for liquid crystals and solids. J Magn Reson (2000) 4.94

Rapid and accurate calculation of protein 1H, 13C and 15N chemical shifts. J Biomol NMR (2003) 4.39

FlgM gains structure in living cells. Proc Natl Acad Sci U S A (2002) 2.46

Toxin-induced conformational changes in a potassium channel revealed by solid-state NMR. Nature (2006) 2.34

Mapping structural interactions using in-cell NMR spectroscopy (STINT-NMR). Nat Methods (2006) 2.15

Determination of membrane protein structure and dynamics by magic-angle-spinning solid-state NMR spectroscopy. J Am Chem Soc (2005) 2.02

Single protein production in living cells facilitated by an mRNA interferase. Mol Cell (2005) 2.00

Protein complexes of the Escherichia coli cell envelope. J Biol Chem (2005) 1.69

Influences of membrane mimetic environments on membrane protein structures. Annu Rev Biophys (2013) 1.58

Membrane protein structure determination using crystallography and lipidic mesophases: recent advances and successes. Biochemistry (2012) 1.57

Structure and interactions of plant cell-wall polysaccharides by two- and three-dimensional magic-angle-spinning solid-state NMR. Biochemistry (2011) 1.57

Secondary structure, dynamics, and topology of a seven-helix receptor in native membranes, studied by solid-state NMR spectroscopy. Angew Chem Int Ed Engl (2007) 1.54

Structure determination of a membrane protein in proteoliposomes. J Am Chem Soc (2012) 1.37

Characterization of membrane proteins in isolated native cellular membranes by dynamic nuclear polarization solid-state NMR spectroscopy without purification and reconstitution. Angew Chem Int Ed Engl (2011) 1.28

Evaluation of parameters critical to observing proteins inside living Escherichia coli by in-cell NMR spectroscopy. J Am Chem Soc (2001) 1.26

Solid-state NMR spectroscopy on cellular preparations enhanced by dynamic nuclear polarization. Angew Chem Int Ed Engl (2012) 1.25

Cellular solid-state nuclear magnetic resonance spectroscopy. Proc Natl Acad Sci U S A (2012) 1.25

Solid-state NMR spectroscopy structure determination of a lipid-embedded heptahelical membrane protein. Nat Methods (2013) 1.20

Cell-free expressed bacteriorhodopsin in different soluble membrane mimetics: biophysical properties and NMR accessibility. Structure (2013) 1.19

Conformation of a seven-helical transmembrane photosensor in the lipid environment. Angew Chem Int Ed Engl (2010) 1.13

Expression screening, protein purification and NMR analysis of human protein domains for structural genomics. J Struct Funct Genomics (2004) 1.13

M2 proton channel structural validation from full-length protein samples in synthetic bilayers and E. coli membranes. Angew Chem Int Ed Engl (2012) 1.12

Pectin-cellulose interactions in the Arabidopsis primary cell wall from two-dimensional magic-angle-spinning solid-state nuclear magnetic resonance. Biochemistry (2012) 1.11

In situ structural characterization of a recombinant protein in native Escherichia coli membranes with solid-state magic-angle-spinning NMR. J Am Chem Soc (2011) 1.11

Nuclear magnetic resonance spectroscopy reveals the functional state of the signalling protein CheY in vivo in Escherichia coli. Mol Microbiol (2003) 1.05

Solid-state NMR spectroscopy applied to a chimeric potassium channel in lipid bilayers. J Am Chem Soc (2008) 1.05

Solid-state NMR on bacterial cells: selective cell wall signal enhancement and resolution improvement using dynamic nuclear polarization. J Am Chem Soc (2013) 1.02

In situ structural studies of Anabaena sensory rhodopsin in the E. coli membrane. Biophys J (2015) 1.02

Structural determinants of specific lipid binding to potassium channels. J Am Chem Soc (2013) 1.00

Enzyme free cloning for high throughput gene cloning and expression. J Struct Funct Genomics (2007) 0.99

Importance of lipid-pore loop interface for potassium channel structure and function. Proc Natl Acad Sci U S A (2013) 0.93

Characterization of membrane protein function by solid-state NMR spectroscopy. Curr Opin Struct Biol (2014) 0.92

Crystal structure of Escherichia coli YidC, a membrane protein chaperone and insertase. Sci Rep (2014) 0.91

NMR-based structural biology enhanced by dynamic nuclear polarization at high magnetic field. J Biomol NMR (2014) 0.91

In-Cell NMR spectroscopy: inhibition of autologous protein expression reduces Escherichia coli lysis. Cell Biochem Biophys (2006) 0.91

Multidimensional solid-state NMR studies of the structure and dynamics of pectic polysaccharides in uniformly 13C-labeled Arabidopsis primary cell walls. Magn Reson Chem (2012) 0.91

Solid-state NMR-based approaches for supramolecular structure elucidation. Acc Chem Res (2013) 0.90

Probing a cell-embedded megadalton protein complex by DNP-supported solid-state NMR. Nat Methods (2015) 0.89

Efficient membrane assembly of the KcsA potassium channel in Escherichia coli requires the protonmotive force. EMBO Rep (2000) 0.87

In situ solid-state NMR spectroscopy of protein in heterogeneous membranes: the baseplate antenna complex of Chlorobaculum tepidum. Angew Chem Int Ed Engl (2012) 0.86

Cellular solid-state NMR investigation of a membrane protein using dynamic nuclear polarization. Biochim Biophys Acta (2014) 0.86

Structure and dynamics of Brachypodium primary cell wall polysaccharides from two-dimensional (13)C solid-state nuclear magnetic resonance spectroscopy. Biochemistry (2014) 0.84

Magic-angle-spinning solid-state NMR of membrane proteins. Methods Enzymol (2015) 0.83

Suppression of phospholipid biosynthesis by cerulenin in the condensed Single-Protein-Production (cSPP) system. J Biomol NMR (2011) 0.82

Solid state NMR and protein-protein interactions in membranes. Curr Opin Struct Biol (2013) 0.82

Insight into the conformational stability of membrane-embedded BamA using a combined solution and solid-state NMR approach. J Biomol NMR (2015) 0.82

Solid-state NMR ¹³C and ¹⁵N resonance assignments of a seven-transmembrane helical protein Anabaena Sensory Rhodopsin. Biomol NMR Assign (2012) 0.81

Rapid prediction of multi-dimensional NMR data sets. J Biomol NMR (2012) 0.79

A solid view of membrane proteins in situ. Biophys J (2015) 0.77

Selectively labeling the heterologous protein in Escherichia coli for NMR studies: a strategy to speed up NMR spectroscopy. J Magn Reson (2001) 0.76

Molecular-level secondary structure, polymorphism, and dynamics of full-length alpha-synuclein fibrils studied by solid-state NMR. Proc Natl Acad Sci U S A (2005) 3.37

Toxin-induced conformational changes in a potassium channel revealed by solid-state NMR. Nature (2006) 2.34

Pre-fibrillar alpha-synuclein variants with impaired beta-structure increase neurotoxicity in Parkinson's disease models. EMBO J (2009) 2.09

Determination of membrane protein structure and dynamics by magic-angle-spinning solid-state NMR spectroscopy. J Am Chem Soc (2005) 2.02

Structural constraints from proton-mediated rare-spin correlation spectroscopy in rotating solids. J Am Chem Soc (2002) 1.68

The structure of the XPF-ssDNA complex underscores the distinct roles of the XPF and ERCC1 helix- hairpin-helix domains in ss/ds DNA recognition. Structure (2012) 1.54

Secondary structure, dynamics, and topology of a seven-helix receptor in native membranes, studied by solid-state NMR spectroscopy. Angew Chem Int Ed Engl (2007) 1.54

A concept for rapid protein-structure determination by solid-state NMR spectroscopy. Angew Chem Int Ed Engl (2005) 1.53

3D structure determination of the Crh protein from highly ambiguous solid-state NMR restraints. J Am Chem Soc (2008) 1.46

Solid-state NMR spectroscopy on complex biomolecules. Angew Chem Int Ed Engl (2010) 1.39

A structural link between inactivation and block of a K+ channel. Nat Struct Mol Biol (2008) 1.36

Protein refolding is required for assembly of the type three secretion needle. Nat Struct Mol Biol (2010) 1.36

Systematic analysis of barrier-forming FG hydrogels from Xenopus nuclear pore complexes. EMBO J (2012) 1.27

Amyloid-like interactions within nucleoporin FG hydrogels. Proc Natl Acad Sci U S A (2010) 1.26

Coupling of activation and inactivation gate in a K+-channel: potassium and ligand sensitivity. EMBO J (2009) 1.26

Cellular solid-state nuclear magnetic resonance spectroscopy. Proc Natl Acad Sci U S A (2012) 1.25

Solid-state NMR spectroscopy on cellular preparations enhanced by dynamic nuclear polarization. Angew Chem Int Ed Engl (2012) 1.25

Structural rearrangements of membrane proteins probed by water-edited solid-state NMR spectroscopy. J Am Chem Soc (2009) 1.23

Analysis of proton-proton transfer dynamics in rotating solids and their use for 3D structure determination. J Am Chem Soc (2003) 1.21

Solid state NMR sequential resonance assignments and conformational analysis of the 2x10.4 kDa dimeric form of the Bacillus subtilis protein Crh. J Biomol NMR (2003) 1.21

Plasticity of the PAS domain and a potential role for signal transduction in the histidine kinase DcuS. Nat Struct Mol Biol (2008) 1.17

Solid-state NMR reveals structural differences between fibrils of wild-type and disease-related A53T mutant alpha-synuclein. J Mol Biol (2008) 1.16

Characterization of Alzheimer's-like paired helical filaments from the core domain of tau protein using solid-state NMR spectroscopy. J Am Chem Soc (2008) 1.15

The conformation of neurotensin bound to its G protein-coupled receptor. Proc Natl Acad Sci U S A (2003) 1.14

Structural characterization of polyglutamine fibrils by solid-state NMR spectroscopy. J Mol Biol (2011) 1.11

High-resolution solid-state NMR studies on uniformly [13C,15N]-labeled ubiquitin. Chembiochem (2005) 1.11

High-resolution solid-state NMR applied to polypeptides and membrane proteins. Acc Chem Res (2003) 1.10

In support of the BMRB. Nat Struct Mol Biol (2012) 1.09

Correlation of amyloid fibril beta-structure with the unfolded state of alpha-synuclein. Chembiochem (2007) 1.06

Structural properties of the promiscuous VP16 activation domain. Biochemistry (2005) 1.05

Solid-state NMR spectroscopy applied to a chimeric potassium channel in lipid bilayers. J Am Chem Soc (2008) 1.05

β-Sheet core of tau paired helical filaments revealed by solid-state NMR. J Am Chem Soc (2012) 1.03

Specificity and affinity of Lac repressor for the auxiliary operators O2 and O3 are explained by the structures of their protein-DNA complexes. J Mol Biol (2009) 1.01

3D NMR spectroscopy for resonance assignment and structure elucidation of proteins under MAS: novel pulse schemes and sensitivity considerations. J Magn Reson (2005) 1.01

Structural determinants of specific lipid binding to potassium channels. J Am Chem Soc (2013) 1.00

Structural characterization of Ca(2+)-ATPase-bound phospholamban in lipid bilayers by solid-state nuclear magnetic resonance (NMR) spectroscopy. Biochemistry (2008) 1.00

Enzyme free cloning for high throughput gene cloning and expression. J Struct Funct Genomics (2007) 0.99

Probing molecular interfaces using 2D magic-angle-spinning NMR on protein mixtures with different uniform labeling. J Am Chem Soc (2004) 0.97

Backbone and side chain assignment strategies for multiply labeled membrane peptides and proteins in the solid state. J Magn Reson (2003) 0.97

Analysis of the XPA and ssDNA-binding surfaces on the central domain of human ERCC1 reveals evidence for subfunctionalization. Nucleic Acids Res (2007) 0.95

Importance of lipid-pore loop interface for potassium channel structure and function. Proc Natl Acad Sci U S A (2013) 0.93

Structure and DNA binding of the human Rtf1 Plus3 domain. Structure (2008) 0.92

The molecular mechanism of toxin-induced conformational changes in a potassium channel: relation to C-type inactivation. Structure (2008) 0.92

Complex formation and light activation in membrane-embedded sensory rhodopsin II as seen by solid-state NMR spectroscopy. Structure (2010) 0.92

NMR-based structural biology enhanced by dynamic nuclear polarization at high magnetic field. J Biomol NMR (2014) 0.91

Structural dynamics of bacterial translation initiation factor IF2. J Biol Chem (2012) 0.91

Solid-state NMR on a large multidomain integral membrane protein: the outer membrane protein assembly factor BamA. J Am Chem Soc (2011) 0.90

Solid-state NMR-based approaches for supramolecular structure elucidation. Acc Chem Res (2013) 0.90

Quantitative analysis of the water occupancy around the selectivity filter of a K+ channel in different gating modes. J Am Chem Soc (2014) 0.89

Protein dynamics detected in a membrane-embedded potassium channel using two-dimensional solid-state NMR spectroscopy. Biochim Biophys Acta (2009) 0.89

Comparative analysis of NMR chemical shift predictions for proteins in the solid phase. Solid State Nucl Magn Reson (2009) 0.88

Altered specificity in DNA binding by the lac repressor: a mutant lac headpiece that mimics the gal repressor. Chembiochem (2005) 0.88

Interaction of epothilone B (patupilone) with microtubules as detected by two-dimensional solid-state NMR spectroscopy. Angew Chem Int Ed Engl (2010) 0.88

Interactions between the toxin Kid of the bacterial parD system and the antitoxins Kis and MazE. Proteins (2007) 0.87

Expression of protein complexes using multiple Escherichia coli protein co-expression systems: a benchmarking study. J Struct Biol (2011) 0.87

Solution structure of the human ubiquitin-specific protease 15 DUSP domain. J Biol Chem (2005) 0.86

High-resolution 3D structure determination of kaliotoxin by solid-state NMR spectroscopy. PLoS One (2008) 0.86

Sequence-independent control of peptide conformation in liposomal vaccines for targeting protein misfolding diseases. J Biol Chem (2011) 0.86

Π-π stacking increases the stability and loading capacity of thermosensitive polymeric micelles for chemotherapeutic drugs. Biomacromolecules (2013) 0.86

The HhH domain of the human DNA repair protein XPF forms stable homodimers. Proteins (2008) 0.85

Solution structure and characterization of the DNA-binding activity of the B3BP-Smr domain. J Mol Biol (2008) 0.85

Probing molecular motion by double-quantum (13C,13C) solid-state NMR spectroscopy: application to ubiquitin. J Am Chem Soc (2010) 0.84

Gradual phosphorylation regulates PC4 coactivator function. FEBS J (2006) 0.84

Structural characterization of Spo0E-like protein-aspartic acid phosphatases that regulate sporulation in bacilli. J Biol Chem (2006) 0.84

Supramolecular structure of membrane-associated polypeptides by combining solid-state NMR and molecular dynamics simulations. Biophys J (2012) 0.84

Structural constraints for the Crh protein from solid-state NMR experiments. J Biomol NMR (2008) 0.84

The tandem zinc-finger region of human ZHX adopts a novel C2H2 zinc finger structure with a C-terminal extension. Biochemistry (2009) 0.83

Platinum-promoted Ga/Al₂O₃ as highly active, selective, and stable catalyst for the dehydrogenation of propane. Angew Chem Int Ed Engl (2014) 0.83

Studying molecular 3D structure and dynamics by high-resolution solid-state NMR: Application to L-tyrosine-ethylester. J Phys Chem A (2005) 0.82

Characterization of folding intermediates of a domain-swapped protein by solid-state NMR spectroscopy. J Am Chem Soc (2007) 0.82

Proton clouds to measure long-range contacts between nonexchangeable side chain protons in solid-state NMR. J Am Chem Soc (2014) 0.82

Dynamic nuclear polarization NMR spectroscopy: revealing multiple conformations in lipid-anchored peptide vaccines. Angew Chem Int Ed Engl (2013) 0.82

Insight into the conformational stability of membrane-embedded BamA using a combined solution and solid-state NMR approach. J Biomol NMR (2015) 0.82

Enhanced spectral resolution in immobilized peptides and proteins by combining chemical shift sum and difference spectroscopy. J Magn Reson (2002) 0.81

The intrinsically unstructured domain of PC4 modulates the activity of the structured core through inter- and intramolecular interactions. Biochemistry (2006) 0.81

Probing conformational disorder in neurotensin by two-dimensional solid-state NMR and comparison to molecular dynamics simulations. Biophys J (2005) 0.81

Novel structural features in two ZHX homeodomains derived from a systematic study of single and multiple domains. BMC Struct Biol (2010) 0.81

Two-dimensional solid-state NMR applied to a chimeric potassium channel. J Recept Signal Transduct Res (2006) 0.80

The Fanconi anemia associated protein FAAP24 uses two substrate specific binding surfaces for DNA recognition. Nucleic Acids Res (2013) 0.79

Rapid prediction of multi-dimensional NMR data sets. J Biomol NMR (2012) 0.79

Protein oligomers studied by solid-state NMR--the case of the full-length nucleoid-associated protein histone-like nucleoid structuring protein. FEBS J (2013) 0.78

Disaccharides impact the lateral organization of lipid membranes. J Am Chem Soc (2014) 0.78

High-resolution solid-state NMR structure of an anticancer agent. ChemMedChem (2007) 0.78

Phosphatidylethanolamine-binding proteins, including RKIP, exhibit affinity for phosphodiesterase-5 inhibitors. Chembiochem (2009) 0.78

Novel strategies to overcome expression problems encountered with toxic proteins: application to the production of Lac repressor proteins for NMR studies. Protein Expr Purif (2009) 0.78

Investigation of ligand-receptor systems by high-resolution solid-state NMR: recent progress and perspectives. Arch Pharm (Weinheim) (2005) 0.77

Janus-faced aluminum: a demonstration of unique Lewis acid and Lewis base behavior of the aluminum atom in [LALB(C6F5)3]. Angew Chem Int Ed Engl (2005) 0.77

Multiple-spin analysis of chemical-shift-selective (13C, 13C) transfer in uniformly labeled biomolecules. J Magn Reson (2004) 0.77

Solid-state NMR [13C,15N] resonance assignments of the nucleotide-binding domain of a bacterial cyclic nucleotide-gated channel. Biomol NMR Assign (2012) 0.76

Structural insights into transcription complexes. J Struct Biol (2011) 0.76

Fractional deuteration applied to biomolecular solid-state NMR spectroscopy. J Biomol NMR (2011) 0.76

Looped structure of flowerlike micelles revealed by 1H NMR relaxometry and light scattering. Langmuir (2011) 0.76

Kinetic analysis of protein aggregation monitored by real-time 2D solid-state NMR spectroscopy. J Biomol NMR (2011) 0.76

NMR characterization of foldedness for the production of E3 RING domains. J Struct Biol (2010) 0.75

Simultaneous use of solution, solid-state NMR and X-ray crystallography to study the conformational landscape of the Crh protein during oligomerization and crystallization. Adv Appl Bioinform Chem (2010) 0.75