FlgM gains structure in living cells.

PubWeight™: 2.46‹?› | Rank: Top 2%

🔗 View Article (PMC 130520)

Published in Proc Natl Acad Sci U S A on September 23, 2002

Authors

Matthew M Dedmon1, Chetan N Patel, Gregory B Young, Gary J Pielak

Author Affiliations

1: Departments of Chemistry, and Biochemistry and Biophysics, and Lineberger Cancer Research Center, University of North Carolina, Chapel Hill, NC 27599, USA.

Articles citing this

GlobPlot: Exploring protein sequences for globularity and disorder. Nucleic Acids Res (2003) 5.90

Linking folding and binding. Curr Opin Struct Biol (2009) 5.19

Understanding protein non-folding. Biochim Biophys Acta (2010) 4.33

Intrinsic disorder is a common feature of hub proteins from four eukaryotic interactomes. PLoS Comput Biol (2006) 3.23

The unfoldomics decade: an update on intrinsically disordered proteins. BMC Genomics (2008) 2.72

High-resolution multi-dimensional NMR spectroscopy of proteins in human cells. Nature (2009) 2.06

An experimental study of GFP-based FRET, with application to intrinsically unstructured proteins. Protein Sci (2007) 1.82

Structure, function, and folding of phosphoglycerate kinase are strongly perturbed by macromolecular crowding. Proc Natl Acad Sci U S A (2010) 1.75

Molecular crowding enhances native structure and stability of alpha/beta protein flavodoxin. Proc Natl Acad Sci U S A (2007) 1.75

Protein structure determination in living cells by in-cell NMR spectroscopy. Nature (2009) 1.71

Quantitative NMR analysis of the protein G B1 domain in Xenopus laevis egg extracts and intact oocytes. Proc Natl Acad Sci U S A (2006) 1.70

Intrinsic disorder in scaffold proteins: getting more from less. Prog Biophys Mol Biol (2008) 1.49

Differential dynamical effects of macromolecular crowding on an intrinsically disordered protein and a globular protein: implications for in-cell NMR spectroscopy. J Am Chem Soc (2008) 1.49

Protein (19)F NMR in Escherichia coli. J Am Chem Soc (2010) 1.46

Volume exclusion and soft interaction effects on protein stability under crowded conditions. Biochemistry (2010) 1.37

Life in a crowded world. EMBO Rep (2004) 1.33

In vivo protein interaction network identified with a novel real-time cross-linked peptide identification strategy. J Proteome Res (2013) 1.32

Protein stability and folding kinetics in the nucleus and endoplasmic reticulum of eucaryotic cells. Biophys J (2011) 1.27

Protein nuclear magnetic resonance under physiological conditions. Biochemistry (2009) 1.23

Macromolecular crowding fails to fold a globular protein in cells. J Am Chem Soc (2011) 1.21

Protein folding: then and now. Arch Biochem Biophys (2007) 1.18

Temperature-induced reversible conformational change in the first 100 residues of alpha-synuclein. Protein Sci (2006) 1.14

Mimicking the plant cell interior under water stress by macromolecular crowding: disordered dehydrin proteins are highly resistant to structural collapse. Plant Physiol (2008) 1.13

The effects of macromolecular crowding on the mechanical stability of protein molecules. Protein Sci (2008) 1.12

Temperature dependence of protein folding kinetics in living cells. Proc Natl Acad Sci U S A (2012) 1.11

In-cell NMR spectroscopy of proteins inside Xenopus laevis oocytes. J Biomol NMR (2006) 1.10

Physicochemical properties of cells and their effects on intrinsically disordered proteins (IDPs). Chem Rev (2014) 1.10

Folding, stability and shape of proteins in crowded environments: experimental and computational approaches. Int J Mol Sci (2009) 1.08

Influence of crowded cellular environments on protein folding, binding, and oligomerization: biological consequences and potentials of atomistic modeling. FEBS Lett (2013) 1.07

The molecular chaperone Hsp90 modulates intermediate steps of amyloid assembly of the Parkinson-related protein alpha-synuclein. J Biol Chem (2009) 1.05

From water and ions to crowded biomacromolecules: in vivo structuring of a prokaryotic cell. Microbiol Mol Biol Rev (2011) 1.03

Single-molecule spectroscopy reveals polymer effects of disordered proteins in crowded environments. Proc Natl Acad Sci U S A (2014) 1.03

Extraordinarily slow binding of guanosine to the Tetrahymena group I ribozyme: implications for RNA preorganization and function. Proc Natl Acad Sci U S A (2003) 1.01

Protein flexibility, not disorder, is intrinsic to molecular recognition. F1000 Biol Rep (2013) 1.01

Site-specific labeling of proteins with NMR-active unnatural amino acids. J Biomol NMR (2009) 1.00

A model of intracellular organization. Proc Natl Acad Sci U S A (2005) 0.99

Structural basis for the interaction of unstructured neuron specific substrates neuromodulin and neurogranin with Calmodulin. Sci Rep (2013) 0.98

Effects of recombinant protein expression on green fluorescent protein diffusion in Escherichia coli. Biochemistry (2009) 0.98

What macromolecular crowding can do to a protein. Int J Mol Sci (2014) 0.96

The Helicobacter pylori anti-sigma factor FlgM is predominantly cytoplasmic and cooperates with the flagellar basal body protein FlhA. J Bacteriol (2009) 0.96

Macromolecular crowding induces a molten globule state in the C-terminal domain of histone H1. Biophys J (2007) 0.96

MetJ repressor interactions with DNA probed by in-cell NMR. Proc Natl Acad Sci U S A (2009) 0.94

In-cell protein NMR and protein leakage. Proteins (2011) 0.93

Effects of molecular crowding on the dynamics of intrinsically disordered proteins. PLoS One (2012) 0.93

Impact of macromolecular crowding on DNA replication. Nat Commun (2013) 0.90

Extracellular structure of polysialic acid explored by on cell solution NMR. Proc Natl Acad Sci U S A (2007) 0.90

Protein folding and misfolding on surfaces. Int J Mol Sci (2008) 0.88

Peeking into living eukaryotic cells with high-resolution NMR. Proc Natl Acad Sci U S A (2006) 0.88

In-cell NMR in E. coli to monitor maturation steps of hSOD1. PLoS One (2011) 0.85

Hydrogen exchange of disordered proteins in Escherichia coli. Protein Sci (2015) 0.85

Factor inhibiting HIF (FIH) recognizes distinct molecular features within hypoxia-inducible factor-α (HIF-α) versus ankyrin repeat substrates. J Biol Chem (2012) 0.84

FlgM anti-sigma factors: identification of novel members of the family, evolutionary analysis, homology modeling, and analysis of sequence-structure-function relationships. J Mol Model (2006) 0.83

Denatured state structural property determines protein stabilization by macromolecular crowding: a thermodynamic and structural approach. PLoS One (2013) 0.83

Self-organization and regulation of intrinsically disordered proteins with folded N-termini. PLoS Biol (2011) 0.82

Unraveling the nature of the segmentation clock: Intrinsic disorder of clock proteins and their interaction map. Comput Biol Chem (2006) 0.81

Protein disorder in plants: a view from the chloroplast. BMC Plant Biol (2012) 0.81

A physics-based approach of coarse-graining the cytoplasm of Escherichia coli (CGCYTO). Biophys J (2012) 0.81

A Unique Tool for Cellular Structural Biology: In-cell NMR. J Biol Chem (2015) 0.81

Structural disorder within Henipavirus nucleoprotein and phosphoprotein: from predictions to experimental assessment. PLoS One (2010) 0.81

Curcumin inhibits HIV-1 by promoting Tat protein degradation. Sci Rep (2016) 0.80

FlgM proteins from different bacteria exhibit different structural characteristics. Biochim Biophys Acta (2013) 0.80

In-cell ¹³C NMR spectroscopy for the study of intrinsically disordered proteins. Nat Protoc (2014) 0.80

Mechanism for pH-dependent gene regulation by amino-terminus-mediated homooligomerization of Bacillus subtilis anti-trp RNA-binding attenuation protein. Proc Natl Acad Sci U S A (2010) 0.79

Backbone assignment and dynamics of human α-synuclein in viscous 2 M glucose solution. Biomol NMR Assign (2010) 0.79

Efficient cellular solid-state NMR of membrane proteins by targeted protein labeling. J Biomol NMR (2015) 0.78

SAXS/SANS probe of intermolecular interactions in concentrated protein solutions. Biophys J (2014) 0.78

Soft interactions and crowding. Biophys Rev (2013) 0.76

Dynamic footprint of sequestration in the molecular fluctuations of osteopontin. J R Soc Interface (2015) 0.76

The transcriptional repressor domain of Gli3 is intrinsically disordered. PLoS One (2013) 0.76

Molecular Effects of Concentrated Solutes on Protein Hydration, Dynamics, and Electrostatics. Biophys J (2016) 0.76

In-cell NMR: a topical review. IUCrJ (2017) 0.75

The role of semidisorder in temperature adaptation of bacterial FlgM proteins. Biophys J (2013) 0.75

Flexibility of the cytoplasmic domain of the phototaxis transducer II from Natronomonas pharaonis. J Biophys (2008) 0.75

A cell is more than the sum of its (dilute) parts: A brief history of quinary structure. Protein Sci (2016) 0.75

Effects of macromolecular crowding agents on protein folding in vitro and in silico. Biophys Rev (2013) 0.75

To be disordered or not to be disordered: is that still a question for proteins in the cell? Cell Mol Life Sci (2017) 0.75

Articles cited by this

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem (1976) 903.81

Principles that govern the folding of protein chains. Science (1973) 23.40

Dominant forces in protein folding. Biochemistry (1990) 15.34

Intrinsic disorder and protein function. Biochemistry (2002) 8.54

Macromolecular crowding: obvious but underappreciated. Trends Biochem Sci (2001) 8.15

Why are "natively unfolded" proteins unstructured under physiologic conditions? Proteins (2000) 7.61

The influence of macromolecular crowding and macromolecular confinement on biochemical reactions in physiological media. J Biol Chem (2001) 5.57

Sensing structural intermediates in bacterial flagellar assembly by export of a negative regulator. Science (1993) 5.03

Analysis of effects of salts and uncharged solutes on protein and nucleic acid equilibria and processes: a practical guide to recognizing and interpreting polyelectrolyte effects, Hofmeister effects, and osmotic effects of salts. Adv Protein Chem (1998) 3.30

Mutual synergistic folding in recruitment of CBP/p300 by p160 nuclear receptor coactivators. Nature (2002) 2.78

Osmolyte-driven contraction of a random coil protein. Proc Natl Acad Sci U S A (1998) 2.22

Structural properties of an amyloid precursor of beta(2)-microglobulin. Nat Struct Biol (2002) 2.21

The C-terminal half of the anti-sigma factor, FlgM, becomes structured when bound to its target, sigma 28. Nat Struct Biol (1997) 1.86

A residue-specific NMR view of the non-cooperative unfolding of a molten globule. Nat Struct Biol (1997) 1.76

Interpreting the effects of small uncharged solutes on protein-folding equilibria. Annu Rev Biophys Biomol Struct (2001) 1.66

Protein folding--what's the question? Proc Natl Acad Sci U S A (1993) 1.59

Trimethylamine N-oxide-induced cooperative folding of an intrinsically unfolded transcription-activating fragment of human glucocorticoid receptor. J Biol Chem (1999) 1.31

Osmolyte-induced changes in protein conformational equilibria. Biopolymers (2000) 1.26

Evaluation of parameters critical to observing proteins inside living Escherichia coli by in-cell NMR spectroscopy. J Am Chem Soc (2001) 1.26

Effects of macromolecular crowding on the intrinsically disordered proteins c-Fos and p27(Kip1). Biomacromolecules (2001) 1.26

Alpha-lactalbumin forms a compact molten globule in the absence of disulfide bonds. Nat Struct Biol (1999) 1.21

The C-terminal half of the anti-sigma factor FlgM contains a dynamic equilibrium solution structure favoring helical conformations. Biochemistry (1998) 1.15

Temperature behaviour of human serum albumin. Eur J Biochem (1980) 1.06

Sugar-induced molten-globule model. Biochemistry (1998) 1.04

Articles by these authors

Macromolecular crowding in the Escherichia coli periplasm maintains alpha-synuclein disorder. J Mol Biol (2005) 2.01

Effects of proteins on protein diffusion. J Am Chem Soc (2010) 1.58

Differential dynamical effects of macromolecular crowding on an intrinsically disordered protein and a globular protein: implications for in-cell NMR spectroscopy. J Am Chem Soc (2008) 1.49

Protein crowding tunes protein stability. J Am Chem Soc (2011) 1.49

Protein (19)F NMR in Escherichia coli. J Am Chem Soc (2010) 1.46

Impact of protein denaturants and stabilizers on water structure. J Am Chem Soc (2004) 1.39

Asymmetric binding between SecA and SecB two symmetric proteins: implications for function in export. J Mol Biol (2005) 1.39

Macromolecular crowding and protein stability. J Am Chem Soc (2012) 1.38

The cellular environment stabilizes adenine riboswitch RNA structure. Biochemistry (2013) 1.38

Volume exclusion and soft interaction effects on protein stability under crowded conditions. Biochemistry (2010) 1.37

Residue-level interrogation of macromolecular crowding effects on protein stability. J Am Chem Soc (2008) 1.33

Macromolecular crowding fails to fold a globular protein in cells. J Am Chem Soc (2011) 1.21

Impact of reconstituted cytosol on protein stability. Proc Natl Acad Sci U S A (2013) 1.20

Indirect inhibition of 26S proteasome activity in a cellular model of Huntington's disease. J Cell Biol (2012) 1.18

Unexpected effects of macromolecular crowding on protein stability. Biochemistry (2012) 1.17

Temperature-induced reversible conformational change in the first 100 residues of alpha-synuclein. Protein Sci (2006) 1.14

In situ structural characterization of a recombinant protein in native Escherichia coli membranes with solid-state magic-angle-spinning NMR. J Am Chem Soc (2011) 1.11

Design of a ruthenium-labeled cytochrome c derivative to study electron transfer with the cytochrome bc1 complex. Biochemistry (2003) 1.10

Physicochemical properties of cells and their effects on intrinsically disordered proteins (IDPs). Chem Rev (2014) 1.10

Retracted Protein dynamics in living cells. Biochemistry (2005) 1.10

Stability and apoptotic activity of recombinant human cytochrome c. Biochem Biophys Res Commun (2003) 1.06

Translational and rotational diffusion of a small globular protein under crowded conditions. J Phys Chem B (2009) 1.05

19F NMR studies of alpha-synuclein conformation and fibrillation. Biochemistry (2009) 1.04

Using NMR to distinguish viscosity effects from nonspecific protein binding under crowded conditions. J Am Chem Soc (2009) 1.02

Effects of recombinant protein expression on green fluorescent protein diffusion in Escherichia coli. Biochemistry (2009) 0.98

19F NMR studies of α-synuclein-membrane interactions. Protein Sci (2010) 0.97

Internal and global protein motion assessed with a fusion construct and in-cell NMR spectroscopy. Chembiochem (2010) 0.94

In-cell protein NMR and protein leakage. Proteins (2011) 0.93

Interaction of α-synuclein with vesicles that mimic mitochondrial membranes. Biochim Biophys Acta (2011) 0.93

High-precision, high-throughput stability determinations facilitated by robotics and a semiautomated titrating fluorometer. Biochemistry (2003) 0.92

Protein crowder charge and protein stability. Biochemistry (2014) 0.91

Using NMR-detected backbone amide 1H exchange to assess macromolecular crowding effects on globular-protein stability. Methods Enzymol (2009) 0.91

Retraction. Biochemistry (2007) 0.91

Quantifying green fluorescent protein diffusion in Escherichia coli by using continuous photobleaching with evanescent illumination. J Phys Chem B (2009) 0.90

(19) F NMR spectroscopy as a probe of cytoplasmic viscosity and weak protein interactions in living cells. Chemistry (2013) 0.88

Peeking into living eukaryotic cells with high-resolution NMR. Proc Natl Acad Sci U S A (2006) 0.88

Amide proton exchange of a dynamic loop in cell extracts. Protein Sci (2013) 0.87

A bioreactor for in-cell protein NMR. J Magn Reson (2009) 0.86

Alpha-Synuclein conformation affects its tyrosine-dependent oxidative aggregation. Biochemistry (2008) 0.86

Searching for quantitative entropy-enthalpy compensation among protein variants. Proteins (2002) 0.86

Probing the micelle-bound aggregation-prone state of α-synuclein with (19)F NMR spectroscopy. Chembiochem (2010) 0.85

Hydrogen exchange of disordered proteins in Escherichia coli. Protein Sci (2015) 0.85

Effects of molecular crowding by saccharides on alpha-chymotrypsin dimerization. Protein Sci (2002) 0.83

Retracted Cytosol has a small effect on protein backbone dynamics. Biochemistry (2006) 0.82

Peroxidative aggregation of alpha-synuclein requires tyrosines. Protein Sci (2004) 0.82

Disordered Protein Diffusion under Crowded Conditions. J Phys Chem Lett (2012) 0.82

Development and cardiac contractility: cardiac troponin T isoforms and cytosolic calcium in rabbit. Pediatr Res (2006) 0.79

An upper limit for macromolecular crowding effects. BMC Biophys (2011) 0.79

Crowding by trisaccharides and the 2:1 cytochrome c-cytochrome c peroxidase complex. Biochemistry (2002) 0.78

Interaction of α-synuclein and a cell penetrating fusion peptide with higher eukaryotic cell membranes assessed by ¹⁹F NMR. Mol Pharm (2012) 0.78

Testing hypotheses about determinants of protein structure with high-precision, high-throughput stability measurements and statistical modeling. Biochemistry (2003) 0.78

Reconsideration of sedimentation equilibrium distributions reflecting the effects of small inert cosolutes on the dimerization of alpha-chymotrypsin. Biophys Chem (2007) 0.78

An osmolyte mitigates the destabilizing effect of protein crowding. Protein Sci (2014) 0.76

Effect of oxygen concentration on viability and metabolism in a fluidized-bed bioartificial liver using ³¹P and ¹³C NMR spectroscopy. Tissue Eng Part C Methods (2012) 0.76

Pressure perturbation calorimetry of helical peptides. Proteins (2006) 0.76

Strategies for protein NMR in Escherichia coli. Biochemistry (2014) 0.76

Unusual Side Effects of Systemic 5-fluorouracil. Indian J Dermatol Venereol Leprol (2017) 0.75

Cosolutes, Crowding and Protein Folding Kinetics. J Phys Chem B (2017) 0.75