Published in Front Genet on September 24, 2015
Image-based siRNA screen to identify kinases regulating Weibel-Palade body size control using electroporation. Sci Data (2017) 0.90
Screening out irrelevant cell-based models of disease. Nat Rev Drug Discov (2016) 0.83
Transcriptional regulation with CRISPR-Cas9: principles, advances, and applications. Curr Opin Biotechnol (2016) 0.77
Genome-Edited T Cell Therapies. Curr Stem Cell Rep (2017) 0.76
Phenotypic screening with primary neurons to identify drug targets for regeneration and degeneration. Mol Cell Neurosci (2016) 0.75
High-throughput in situ cell electroporation microsystem for parallel delivery of single guide RNAs into mammalian cells. Sci Rep (2017) 0.75
CRISPR/Cas9: Transcending the Reality of Genome Editing. Mol Ther Nucleic Acids (2017) 0.75
Fiji: an open-source platform for biological-image analysis. Nat Methods (2012) 56.92
Multiplex genome engineering using CRISPR/Cas systems. Science (2013) 55.53
RNA-guided human genome engineering via Cas9. Science (2013) 48.29
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science (2012) 48.17
CellProfiler: image analysis software for identifying and quantifying cell phenotypes. Genome Biol (2006) 32.51
CRISPR provides acquired resistance against viruses in prokaryotes. Science (2007) 29.74
A lentiviral RNAi library for human and mouse genes applied to an arrayed viral high-content screen. Cell (2006) 18.81
Evolution and classification of the CRISPR-Cas systems. Nat Rev Microbiol (2011) 17.11
RNA-programmed genome editing in human cells. Elife (2013) 14.82
Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression. Cell (2013) 13.43
FLASH assembly of TALENs for high-throughput genome editing. Nat Biotechnol (2012) 12.97
Genome-scale CRISPR-Cas9 knockout screening in human cells. Science (2013) 12.36
CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering. Nat Biotechnol (2013) 11.35
Genetic screens in human cells using the CRISPR-Cas9 system. Science (2013) 10.75
CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes. Cell (2013) 9.55
TALENs: a widely applicable technology for targeted genome editing. Nat Rev Mol Cell Biol (2012) 7.01
BAC TransgeneOmics: a high-throughput method for exploration of protein function in mammals. Nat Methods (2008) 6.51
A resource for large-scale RNA-interference-based screens in mammals. Nature (2004) 6.36
A functional genomic analysis of cell morphology using RNA interference. J Biol (2003) 5.99
DNA interrogation by the CRISPR RNA-guided endonuclease Cas9. Nature (2014) 5.70
Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation. Cell (2014) 5.43
Cancer proliferation gene discovery through functional genomics. Science (2008) 5.41
Dynamic imaging of genomic loci in living human cells by an optimized CRISPR/Cas system. Cell (2013) 5.40
In vivo genome editing using Staphylococcus aureus Cas9. Nature (2015) 5.26
Crystal structure of Cas9 in complex with guide RNA and target DNA. Cell (2014) 5.26
Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Nature (2014) 5.19
Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells. Nat Biotechnol (2014) 5.07
High-throughput screening of a CRISPR/Cas9 library for functional genomics in human cells. Nature (2014) 4.95
High-throughput RNAi screening by time-lapse imaging of live human cells. Nat Methods (2006) 4.86
Genome-wide recessive genetic screening in mammalian cells with a lentiviral CRISPR-guide RNA library. Nat Biotechnol (2013) 4.72
CRISPR RNA-guided activation of endogenous human genes. Nat Methods (2013) 4.69
Simple methods for generating and detecting locus-specific mutations induced with TALENs in the zebrafish genome. PLoS Genet (2012) 4.42
Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing. Nat Biotechnol (2014) 4.41
Genome-wide analysis reveals characteristics of off-target sites bound by the Cas9 endonuclease. Nat Biotechnol (2014) 4.16
Image-based multivariate profiling of drug responses from single cells. Nat Methods (2007) 3.90
Engineered CRISPR-Cas9 nucleases with altered PAM specificities. Nature (2015) 3.77
Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification. Nat Biotechnol (2014) 3.72
CRISPR-Cas9 knockin mice for genome editing and cancer modeling. Cell (2014) 3.68
Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation. Nat Biotechnol (2014) 3.66
Genome-wide CRISPR screen in a mouse model of tumor growth and metastasis. Cell (2015) 3.58
Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. Cell Res (2013) 3.50
Unravelling the structural and mechanistic basis of CRISPR-Cas systems. Nat Rev Microbiol (2014) 3.48
Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins. Genome Res (2014) 3.31
Enhanced CellClassifier: a multi-class classification tool for microscopy images. BMC Bioinformatics (2010) 2.84
Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo. Nat Biotechnol (2014) 2.75
E-CRISP: fast CRISPR target site identification. Nat Methods (2014) 2.73
Synthesis of high-quality libraries of long (150mer) oligonucleotides by a novel depurination controlled process. Nucleic Acids Res (2010) 2.72
Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery. Elife (2014) 2.65
A screen for morphological complexity identifies regulators of switch-like transitions between discrete cell shapes. Nat Cell Biol (2013) 2.59
In vivo interrogation of gene function in the mammalian brain using CRISPR-Cas9. Nat Biotechnol (2014) 2.51
Heritable gene knockout in Caenorhabditis elegans by direct injection of Cas9-sgRNA ribonucleoproteins. Genetics (2013) 2.44
CHOPCHOP: a CRISPR/Cas9 and TALEN web tool for genome editing. Nucleic Acids Res (2014) 2.36
High-throughput functional genomics using CRISPR-Cas9. Nat Rev Genet (2015) 2.35
Programmable RNA recognition and cleavage by CRISPR/Cas9. Nature (2014) 2.33
Highly efficient gene knockout in mice and zebrafish with RNA-guided endonucleases. Genome Res (2013) 2.28
A protein-tagging system for signal amplification in gene expression and fluorescence imaging. Cell (2014) 2.27
Single-cell analysis of population context advances RNAi screening at multiple levels. Mol Syst Biol (2012) 2.13
CRISPR interference (CRISPRi) for sequence-specific control of gene expression. Nat Protoc (2013) 2.05
Protospacer recognition motifs: mixed identities and functional diversity. RNA Biol (2013) 2.00
Discovery of cancer drug targets by CRISPR-Cas9 screening of protein domains. Nat Biotechnol (2015) 1.83
Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells. Nat Biotechnol (2015) 1.71
Specific elimination of mutant mitochondrial genomes in patient-derived cells by mitoTALENs. Nat Med (2013) 1.61
CRISPRdirect: software for designing CRISPR/Cas guide RNA with reduced off-target sites. Bioinformatics (2014) 1.57
Multicolor CRISPR labeling of chromosomal loci in human cells. Proc Natl Acad Sci U S A (2015) 1.56
RNAi screening comes of age: improved techniques and complementary approaches. Nat Rev Mol Cell Biol (2014) 1.54
sgRNAcas9: a software package for designing CRISPR sgRNA and evaluating potential off-target cleavage sites. PLoS One (2014) 1.54
Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach. Nat Methods (2015) 1.53
CRISPR multitargeter: a web tool to find common and unique CRISPR single guide RNA targets in a set of similar sequences. PLoS One (2015) 1.51
Quantifying genome-editing outcomes at endogenous loci with SMRT sequencing. Cell Rep (2014) 1.50
A light-inducible CRISPR-Cas9 system for control of endogenous gene activation. Nat Chem Biol (2015) 1.48
The function of introns. Front Genet (2012) 1.42
Repurposing CRISPR/Cas9 for in situ functional assays. Genes Dev (2013) 1.41
Protospacer adjacent motif (PAM)-distal sequences engage CRISPR Cas9 DNA target cleavage. PLoS One (2014) 1.38
Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection. J Biotechnol (2015) 1.37
Increasing the Content of High-Content Screening: An Overview. J Biomol Screen (2014) 1.36
Enhanced specificity and efficiency of the CRISPR/Cas9 system with optimized sgRNA parameters in Drosophila. Cell Rep (2014) 1.35
C911: A bench-level control for sequence specific siRNA off-target effects. PLoS One (2012) 1.32
Genome-wide siRNA screen reveals amino acid starvation-induced autophagy requires SCOC and WAC. EMBO J (2012) 1.31
Rational design of a split-Cas9 enzyme complex. Proc Natl Acad Sci U S A (2015) 1.30
Stable RNA interference: comparison of U6 and H1 promoters in endothelial cells and in mouse brain. J Gene Med (2006) 1.29
High-resolution DNA melt curve analysis of the clustered, regularly interspaced short-palindromic-repeat locus of Campylobacter jejuni. Appl Environ Microbiol (2007) 1.22
Comparison of T7E1 and surveyor mismatch cleavage assays to detect mutations triggered by engineered nucleases. G3 (Bethesda) (2015) 1.13
CRISPR-Cas9-based photoactivatable transcription system. Chem Biol (2015) 1.12
The structural biology of CRISPR-Cas systems. Curr Opin Struct Biol (2015) 1.07
Single-cell and multivariate approaches in genetic perturbation screens. Nat Rev Genet (2014) 1.07
A two-tier Golgi-based control of organelle size underpins the functional plasticity of endothelial cells. Dev Cell (2014) 1.06
Application guide for omics approaches to cell signaling. Nat Chem Biol (2015) 1.04
Development of an intein-mediated split-Cas9 system for gene therapy. Nucleic Acids Res (2015) 1.03
An efficient genotyping method for genome-modified animals and human cells generated with CRISPR/Cas9 system. Sci Rep (2014) 1.02
Novel fluorescent genome editing reporters for monitoring DNA repair pathway utilization at endonuclease-induced breaks. Nucleic Acids Res (2013) 1.02
Pol III Promoters to Express Small RNAs: Delineation of Transcription Initiation. Mol Ther Nucleic Acids (2014) 0.99
Expression of CRISPR/Cas single guide RNAs using small tRNA promoters. RNA (2015) 0.98
A hierarchical map of regulatory genetic interactions in membrane trafficking. Cell (2014) 0.97
Developments in expression cloning. Curr Opin Biotechnol (1995) 0.96
CellProfiler and KNIME: open source tools for high content screening. Methods Mol Biol (2013) 0.94
A high-content imaging workflow to study Grb2 signaling complexes by expression cloning. J Vis Exp (2012) 0.90
Cleavage of mispaired heteroduplex DNA substrates by numerous restriction enzymes. Curr Issues Mol Biol (2008) 0.89
WGE: a CRISPR database for genome engineering. Bioinformatics (2015) 0.89
Fast and sensitive detection of indels induced by precise gene targeting. Nucleic Acids Res (2015) 0.88
A genome-wide CRISPR library for high-throughput genetic screening in Drosophila cells. J Genet Genomics (2015) 0.86