Published in Curr Genet on December 01, 1989
Improved method for high efficiency transformation of intact yeast cells. Nucleic Acids Res (1992) 34.33
The two-hybrid system: a method to identify and clone genes for proteins that interact with a protein of interest. Proc Natl Acad Sci U S A (1991) 15.52
A new efficient gene disruption cassette for repeated use in budding yeast. Nucleic Acids Res (1996) 11.05
Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator that binds to the C-repeat/DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit. Proc Natl Acad Sci U S A (1997) 8.49
Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems. Nucleic Acids Res (2013) 7.77
Cell cycle and genetic requirements of two pathways of nonhomologous end-joining repair of double-strand breaks in Saccharomyces cerevisiae. Mol Cell Biol (1996) 7.33
Lambda YES: a multifunctional cDNA expression vector for the isolation of genes by complementation of yeast and Escherichia coli mutations. Proc Natl Acad Sci U S A (1991) 6.82
A novel iron-regulated metal transporter from plants identified by functional expression in yeast. Proc Natl Acad Sci U S A (1996) 6.45
Identification of a new family of tissue-specific basic helix-loop-helix proteins with a two-hybrid system. Mol Cell Biol (1995) 5.71
WW domains of Nedd4 bind to the proline-rich PY motifs in the epithelial Na+ channel deleted in Liddle's syndrome. EMBO J (1996) 5.24
Multiple molecular determinants for retrotransposition in a primer tRNA. Mol Cell Biol (1995) 4.95
Senescence mutants of Saccharomyces cerevisiae with a defect in telomere replication identify three additional EST genes. Genetics (1996) 4.78
Saccharomyces cerevisiae Ku70 potentiates illegitimate DNA double-strand break repair and serves as a barrier to error-prone DNA repair pathways. EMBO J (1996) 4.58
DMSO-enhanced whole cell yeast transformation. Nucleic Acids Res (1991) 4.52
Identification of a Saccharomyces cerevisiae Ku80 homologue: roles in DNA double strand break rejoining and in telomeric maintenance. Nucleic Acids Res (1996) 4.37
Integration of DNA fragments by illegitimate recombination in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A (1991) 4.18
Synthetic-lethal interactions identify two novel genes, SLA1 and SLA2, that control membrane cytoskeleton assembly in Saccharomyces cerevisiae. J Cell Biol (1993) 4.03
Ctf7p is essential for sister chromatid cohesion and links mitotic chromosome structure to the DNA replication machinery. Genes Dev (1999) 3.94
The Hog1 MAPK prevents cross talk between the HOG and pheromone response MAPK pathways in Saccharomyces cerevisiae. Genes Dev (1998) 3.79
The yeast ZRT1 gene encodes the zinc transporter protein of a high-affinity uptake system induced by zinc limitation. Proc Natl Acad Sci U S A (1996) 3.72
The surveillance complex interacts with the translation release factors to enhance termination and degrade aberrant mRNAs. Genes Dev (1998) 3.71
Molecular cloning of cellular genes encoding retinoblastoma-associated proteins: identification of a gene with properties of the transcription factor E2F. Mol Cell Biol (1992) 3.58
Direct evidence for SIR2 modulation of chromatin structure in yeast rDNA. EMBO J (1997) 3.54
Identification of a family of zinc transporter genes from Arabidopsis that respond to zinc deficiency. Proc Natl Acad Sci U S A (1998) 3.41
Effect of mutations in genes affecting homologous recombination on restriction enzyme-mediated and illegitimate recombination in Saccharomyces cerevisiae. Mol Cell Biol (1994) 3.41
Two different types of double-strand breaks in Saccharomyces cerevisiae are repaired by similar RAD52-independent, nonhomologous recombination events. Mol Cell Biol (1994) 3.40
MMM1 encodes a mitochondrial outer membrane protein essential for establishing and maintaining the structure of yeast mitochondria. J Cell Biol (1994) 3.31
A novel RING finger protein complex essential for a late step in protein transport to the yeast vacuole. Mol Biol Cell (1997) 3.27
Interactions among members of the Bcl-2 protein family analyzed with a yeast two-hybrid system. Proc Natl Acad Sci U S A (1994) 3.26
Cofilin is an essential component of the yeast cortical cytoskeleton. J Cell Biol (1993) 3.23
Micro-homology mediated PCR targeting in Saccharomyces cerevisiae. Nucleic Acids Res (1995) 3.11
Hrs regulates multivesicular body formation via ESCRT recruitment to endosomes. J Cell Biol (2003) 3.07
Mismatch correction acts as a barrier to homeologous recombination in Saccharomyces cerevisiae. Genetics (1995) 3.02
Histidine kinase activity of the ETR1 ethylene receptor from Arabidopsis. Proc Natl Acad Sci U S A (1998) 2.97
Rab-interacting lysosomal protein (RILP): the Rab7 effector required for transport to lysosomes. EMBO J (2001) 2.97
Unique and redundant roles for HOG MAPK pathway components as revealed by whole-genome expression analysis. Mol Biol Cell (2003) 2.92
Parallel secretory pathways to the cell surface in yeast. J Cell Biol (1995) 2.92
The Med proteins of yeast and their function through the RNA polymerase II carboxy-terminal domain. Genes Dev (1998) 2.91
VPS21 encodes a rab5-like GTP binding protein that is required for the sorting of yeast vacuolar proteins. EMBO J (1994) 2.90
Protein-protein interactions in the yeast pheromone response pathway: Ste5p interacts with all members of the MAP kinase cascade. Genetics (1994) 2.83
Yeast Los1p has properties of an exportin-like nucleocytoplasmic transport factor for tRNA. Mol Cell Biol (1998) 2.83
Hrs recruits clathrin to early endosomes. EMBO J (2001) 2.82
Substrate length requirements for efficient mitotic recombination in Saccharomyces cerevisiae. Mol Cell Biol (1993) 2.81
Genetic and biochemical characterization of mutations in the ATPase and helicase regions of the Upf1 protein. Mol Cell Biol (1996) 2.78
The RNA of RNase MRP is required for normal processing of ribosomal RNA. Proc Natl Acad Sci U S A (1994) 2.68
Anaerobic xylose fermentation by recombinant Saccharomyces cerevisiae carrying XYL1, XYL2, and XKS1 in mineral medium chemostat cultures. Appl Environ Microbiol (2000) 2.65
Identification of Prp40, a novel essential yeast splicing factor associated with the U1 small nuclear ribonucleoprotein particle. Mol Cell Biol (1996) 2.65
A role for Vps1p, actin, and the Myo2p motor in peroxisome abundance and inheritance in Saccharomyces cerevisiae. J Cell Biol (2001) 2.64
Upf1p, Nmd2p, and Upf3p are interacting components of the yeast nonsense-mediated mRNA decay pathway. Mol Cell Biol (1997) 2.63
The purified yeast pre-mRNA splicing factor PRP2 is an RNA-dependent NTPase. EMBO J (1992) 2.60
A novel protein complex promoting formation of functional alpha- and gamma-tubulin. EMBO J (1998) 2.58
MLL and CREB bind cooperatively to the nuclear coactivator CREB-binding protein. Mol Cell Biol (2001) 2.58
Assembly of the ER to Golgi SNARE complex requires Uso1p. J Cell Biol (1996) 2.57
A nuclear 3'-5' exonuclease involved in mRNA degradation interacts with Poly(A) polymerase and the hnRNA protein Npl3p. Mol Cell Biol (2000) 2.56
Cloning-free PCR-based allele replacement methods. Genome Res (1997) 2.56
Characterization of cis-acting sequences and decay intermediates involved in nonsense-mediated mRNA turnover. Mol Cell Biol (1995) 2.55
gamma-Tubulin-like Tub4p of Saccharomyces cerevisiae is associated with the spindle pole body substructures that organize microtubules and is required for mitotic spindle formation. J Cell Biol (1996) 2.53
A family of cyclin D homologs from plants differentially controlled by growth regulators and containing the conserved retinoblastoma protein interaction motif. Plant Cell (1995) 2.49
Receptors determine the cellular localization of a gamma-tubulin complex and thereby the site of microtubule formation. EMBO J (1998) 2.48
A family of ADP-ribosylation factor effectors that can alter membrane transport through the trans-Golgi. Mol Biol Cell (2000) 2.45
Lethality induced by a single site-specific double-strand break in a dispensable yeast plasmid. Proc Natl Acad Sci U S A (1993) 2.44
Transcriptional repression by neuron-restrictive silencer factor is mediated via the Sin3-histone deacetylase complex. Mol Cell Biol (2000) 2.36
Identification and characterization of mutations in the UPF1 gene that affect nonsense suppression and the formation of the Upf protein complex but not mRNA turnover. Mol Cell Biol (1996) 2.35
Two survivor pathways that allow growth in the absence of telomerase are generated by distinct telomere recombination events. Mol Cell Biol (2001) 2.34
GAIP, a protein that specifically interacts with the trimeric G protein G alpha i3, is a member of a protein family with a highly conserved core domain. Proc Natl Acad Sci U S A (1995) 2.33
Identification and characterization of a sequence motif involved in nonsense-mediated mRNA decay. Mol Cell Biol (1995) 2.31
Pheromone signalling in Saccharomyces cerevisiae requires the small GTP-binding protein Cdc42p and its activator CDC24. Mol Cell Biol (1995) 2.31
Characterization of three related glucose repressors and genes they regulate in Saccharomyces cerevisiae. Genetics (1998) 2.27
E2F-5, a new E2F family member that interacts with p130 in vivo. Mol Cell Biol (1995) 2.26
The Skn7 response regulator controls gene expression in the oxidative stress response of the budding yeast Saccharomyces cerevisiae. EMBO J (1997) 2.25
The complex of Arl2-GTP and PDE delta: from structure to function. EMBO J (2002) 2.24
A novel Sec18p/NSF-dependent complex required for Golgi-to-endosome transport in yeast. Mol Biol Cell (1997) 2.24
Cell cycle-regulated generation of single-stranded G-rich DNA in the absence of telomerase. Proc Natl Acad Sci U S A (1996) 2.22
Three yeast proteins related to the human candidate tumor suppressor p33(ING1) are associated with histone acetyltransferase activities. Mol Cell Biol (2000) 2.21
A novel allele of Saccharomyces cerevisiae RFA1 that is deficient in recombination and repair and suppressible by RAD52. Mol Cell Biol (1995) 2.21
An examination of adaptive reversion in Saccharomyces cerevisiae. Genetics (1992) 2.21
Oxidative stress is involved in heat-induced cell death in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A (1996) 2.20
Distinct Rab-binding domains mediate the interaction of Rabaptin-5 with GTP-bound Rab4 and Rab5. EMBO J (1998) 2.20
Misexpression of the opaque-phase-specific gene PEP1 (SAP1) in the white phase of Candida albicans confers increased virulence in a mouse model of cutaneous infection. Infect Immun (1999) 2.19
Mss51p and Cox14p jointly regulate mitochondrial Cox1p expression in Saccharomyces cerevisiae. EMBO J (2004) 2.18
Activated Ras interacts with the Ral guanine nucleotide dissociation stimulator. Proc Natl Acad Sci U S A (1994) 2.14
A novel yeast gene, THO2, is involved in RNA pol II transcription and provides new evidence for transcriptional elongation-associated recombination. EMBO J (1998) 2.11
Novel Upf2p orthologues suggest a functional link between translation initiation and nonsense surveillance complexes. Mol Cell Biol (2000) 2.07
Expression and localization of two low molecular weight GTP-binding proteins, Rab8 and Rab10, by epitope tag. Proc Natl Acad Sci U S A (1993) 2.06
Peptide linkage mapping of the Agrobacterium tumefaciens vir-encoded type IV secretion system reveals protein subassemblies. Proc Natl Acad Sci U S A (2002) 2.06
The gene for a major exopolyphosphatase of Saccharomyces cerevisiae. J Bacteriol (1995) 2.06
PICK1: a perinuclear binding protein and substrate for protein kinase C isolated by the yeast two-hybrid system. J Cell Biol (1995) 2.05
RAD53, DUN1 and PDS1 define two parallel G2/M checkpoint pathways in budding yeast. EMBO J (1999) 2.05
hCTR1: a human gene for copper uptake identified by complementation in yeast. Proc Natl Acad Sci U S A (1997) 2.04
Localization of a yeast early Golgi mannosyltransferase, Och1p, involves retrograde transport. J Cell Biol (1996) 2.03
Repression of gene expression by an exogenous sequence element acting in concert with a heterogeneous nuclear ribonucleoprotein-like protein, Nrd1, and the putative helicase Sen1. Mol Cell Biol (1996) 2.02
Sphingoid base signaling via Pkh kinases is required for endocytosis in yeast. EMBO J (2001) 2.02
Novel protein kinases associated with calcineurin B-like calcium sensors in Arabidopsis. Plant Cell (1999) 2.02
Mutations in a protein tyrosine phosphatase gene (PTP2) and a protein serine/threonine phosphatase gene (PTC1) cause a synthetic growth defect in Saccharomyces cerevisiae. Mol Cell Biol (1993) 2.01
Inhibition of G-protein signaling by dominant gain-of-function mutations in Sst2p, a pheromone desensitization factor in Saccharomyces cerevisiae. Mol Cell Biol (1995) 1.99
Regulatory network connecting two glucose signal transduction pathways in Saccharomyces cerevisiae. Eukaryot Cell (2004) 1.99
Shy1p is necessary for full expression of mitochondrial COX1 in the yeast model of Leigh's syndrome. EMBO J (2002) 1.96
The Cdc31p-binding protein Kar1p is a component of the half bridge of the yeast spindle pole body. J Cell Biol (1995) 1.95
BNIP3 heterodimerizes with Bcl-2/Bcl-X(L) and induces cell death independent of a Bcl-2 homology 3 (BH3) domain at both mitochondrial and nonmitochondrial sites. J Biol Chem (2000) 2.68
The E1B 19K/Bcl-2-binding protein Nip3 is a dimeric mitochondrial protein that activates apoptosis. J Exp Med (1997) 2.33
Overlapping transcription units in the dopa decarboxylase region of Drosophila. Nature (1986) 2.28
HIP1, a human homologue of S. cerevisiae Sla2p, interacts with membrane-associated huntingtin in the brain. Nat Genet (1997) 2.09
Sequence and structure of the dopa decarboxylase gene of Drosophila: evidence for novel RNA splicing variants. EMBO J (1986) 1.60
Huntingtin is ubiquitinated and interacts with a specific ubiquitin-conjugating enzyme. J Biol Chem (1996) 1.47
Induction of translatable mRNA for dopa decarboxylase in Drosophila: an early response to ecdysterone. Proc Natl Acad Sci U S A (1980) 1.44
Specificity of the yeast rev3 delta antimutator and REV3 dependency of the mutator resulting from a defect (rad1 delta) in nucleotide excision repair. Genetics (1994) 1.39
Interaction of the yeast RAD7 and SIR3 proteins: implications for DNA repair and chromatin structure. Genes Dev (1994) 1.21
Specificities of the Saccharomyces cerevisiae rad6, rad18, and rad52 mutators exhibit different degrees of dependence on the REV3 gene product, a putative nonessential DNA polymerase. Genetics (1995) 1.15
Identification and characterization of CKIP-1, a novel pleckstrin homology domain-containing protein that interacts with protein kinase CK2. J Biol Chem (2000) 1.08
The C. elegans orthologue ceBNIP3 interacts with CED-9 and CED-3 but kills through a BH3- and caspase-independent mechanism. Oncogene (2000) 1.06
An analysis of dopa decarboxylase expression during embryogenesis in Drosophila melanogaster. Dev Biol (1985) 1.06
Assignment of growth factor receptor-bound protein 10 (GRB10) to human chromosome 7p11.2-p12. Genomics (1997) 0.99
High-efficiency transformation of plasmid DNA into yeast. Methods Mol Biol (2001) 0.99
Carcinogens induce intrachromosomal recombination in yeast. Carcinogenesis (1989) 0.98
Human growth factor receptor bound 14 binds the activated insulin receptor and alters the insulin-stimulated tyrosine phosphorylation levels of multiple proteins. Biochem Cell Biol (2001) 0.95
Interactions of the RAD7 and RAD23 excision repair genes of Saccharomyces cerevisiae with DNA repair genes in different epistasis groups. Curr Genet (1989) 0.89
Production of digoxigenin-labelled RNA probes and the detection of cytokine mRNA in rat spleen and brain by in situ hybridization. Brain Res Brain Res Protoc (1998) 0.88
Analysis of the transcription unit adjacent to the 3'-end of the dopa decarboxylase gene in Drosophila melanogaster. Dev Biol (1986) 0.86
Analysis of interactions between the subunits of protein kinase CK2. Biochem Cell Biol (1996) 0.81
Effect of peroxisome proliferators on intrachromosomal and interchromosomal recombination in yeast. Carcinogenesis (1990) 0.81
Interchromosomal and intrachromosomal recombination in rad 18 mutants of Saccharomyces cerevisiae. Mol Gen Genet (1990) 0.80
A member of the nuclear factor-1 family is involved in the pituitary repression of the human placental growth hormone genes. Biochem J (2001) 0.80
Hormonal aspects of the regulation of dopa decarboxylase in Drosophila melanogaster. Prog Clin Biol Res (1986) 0.78
Safrole, eugenol and methyleugenol induce intrachromosomal recombination in yeast. Mutat Res (1989) 0.77
Rapid screening of plasmid DNA by direct sequencing from bacterial colonies. Biotechniques (2000) 0.76