Published in Proc Natl Acad Sci U S A on November 01, 1979
Selection for animal cells that express the Escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase. Proc Natl Acad Sci U S A (1981) 22.21
A small segment of polyoma virus DNA enhances the expression of a cloned beta-globin gene over a distance of 1400 base pairs. Nucleic Acids Res (1981) 7.57
Construction of poxviruses as cloning vectors: insertion of the thymidine kinase gene from herpes simplex virus into the DNA of infectious vaccinia virus. Proc Natl Acad Sci U S A (1982) 7.04
The construction of cosmid libraries which can be used to transform eukaryotic cells. Nucleic Acids Res (1982) 5.66
DNA sequences preceding the rabbit beta-globin gene are required for formation in mouse L cells of beta-globin RNA with the correct 5' terminus. Proc Natl Acad Sci U S A (1981) 5.26
Bovine papilloma virus deoxyribonucleic acid: a novel eucaryotic cloning vector. Mol Cell Biol (1981) 4.75
Excretion of hepatitis B surface antigen particles from mouse cells transformed with cloned viral DNA. Proc Natl Acad Sci U S A (1980) 3.97
Integration, transcription, and control of a Drosophila heat shock gene in mouse cells. Proc Natl Acad Sci U S A (1981) 3.70
Molecular analysis of the human beta-globin locus activation region. Proc Natl Acad Sci U S A (1989) 3.17
High-frequency transfer of cloned herpes simplex virus type 1 sequences to mammalian cells by protoplast fusion. Mol Cell Biol (1981) 2.97
The human beta-globin gene and a functional viral thymidine kinase gene in developing mice. Proc Natl Acad Sci U S A (1981) 2.93
Developmentally regulated expression of chimeric genes containing muscle actin DNA sequences in transfected myogenic cells. EMBO J (1984) 2.60
Transformation of frog embryos with a rabbit beta-globin gene. Proc Natl Acad Sci U S A (1981) 2.37
Replication and expression of thymidine kinase and human globin genes microinjected into mouse fibroblasts. Proc Natl Acad Sci U S A (1980) 2.10
Gene transfer: DNA microinjection compared with DNA transfection with a very high efficiency. Mol Cell Biol (1982) 1.94
Nucleosomal packaging of the thymidine kinase gene of herpes simplex virus transferred into mouse cells: an actively expressed single-copy gene. Proc Natl Acad Sci U S A (1980) 1.53
Transfer of human globin genes to erythroleukemic mouse cells. EMBO J (1982) 1.41
A negative regulatory sequence near the mouse beta-maj globin gene associated with a region of potential Z-DNA. EMBO J (1984) 1.40
Transcription unit of the rabbit beta 1 globin gene. Mol Cell Biol (1985) 1.40
The immunoglobulin heavy chain enhancer is stimulated by the adenovirus type 2 E1A products in mouse fibroblasts. Proc Natl Acad Sci U S A (1986) 1.39
The gene S promoter of hepatitis B virus confers constitutive gene expression. Nucleic Acids Res (1983) 1.23
Transfer of nonselectable genes into mouse teratocarcinoma cells and transcription of the transferred human beta-globin gene. Mol Cell Biol (1982) 1.19
Introduction and expression of a fetal human globin gene in mouse fibroblasts. Mol Cell Biol (1982) 1.16
Expression and amplification of engineered mouse dihydrofolate reductase minigenes. Mol Cell Biol (1983) 1.15
Cotransfer of circular and linear prokaryotic and eukaryotic DNA sequences into mouse cells. Proc Natl Acad Sci U S A (1980) 1.02
High-level expression of a cloned HLA heavy chain gene introduced into mouse cells on a bovine papillomavirus vector. Mol Cell Biol (1984) 0.99
Genetic variation in rates of antipyrine metabolite formation: a study in uninduced twins. Proc Natl Acad Sci U S A (1981) 0.98
A combination of RNase H and S1 nuclease circumvents an artefact inherent to conventional S1 analysis of RNA splicing. Nucleic Acids Res (1987) 0.96
Construction and use of a dominant, selectable marker: a Harvey sarcoma virus-dihydrofolate reductase chimera. Mol Cell Biol (1983) 0.95
Amplification and expression of human alpha-globin genes in Chinese hamster ovary cells. Mol Cell Biol (1984) 0.94
Regulated expression of a Drosophila melanogaster heat shock locus after stable integration in a Drosophila hydei cell line. Mol Cell Biol (1985) 0.85
Subcellular localization of RNAs in transfected cells: role of sequences at the 5' terminus. Nucleic Acids Res (1984) 0.85
Adenovirus late sequences linked to herpes simplex virus thymidine kinase may be introduced into eukaryotic cells and transcribed. Nucleic Acids Res (1981) 0.81
Gene transfer of truncated NGF receptor clones leads to cell surface expression in mouse fibroblasts. Nucleic Acids Res (1989) 0.80
Transplantation of the human insulin gene into fertilized mouse eggs. EMBO J (1982) 0.80
DNA-mediated gene transfer in Friend leukemia cells by cotransfection of simian virus 40 DNA with herpes simplex virus thymidine kinase DNA. J Virol (1983) 0.75
On the road from classical to modern molecular biology. Nat Med (2012) 0.75
Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol (1975) 503.08
Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids. Cell (1977) 61.24
Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. Proc Natl Acad Sci U S A (1977) 40.24
The isolation of structural genes from libraries of eucaryotic DNA. Cell (1978) 37.13
DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells. Proc Natl Acad Sci U S A (1979) 21.22
Transformation of mammalian cells with genes from procaryotes and eucaryotes. Cell (1979) 21.15
Methylmercury as a reversible denaturing agent for agarose gel electrophoresis. Anal Biochem (1976) 21.07
Biochemical transfer of single-copy eucaryotic genes using total cellular DNA as donor. Cell (1978) 19.44
Transfer of purified herpes virus thymidine kinase gene to cultured mouse cells. Cell (1977) 18.95
Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamide. Nucleic Acids Res (1977) 15.92
Amplification and characterization of a beta-globin gene synthesized in vitro. Cell (1976) 14.99
DELETION OF THYMIDINE KINASE ACTIVITY FROM L CELLS RESISTANT TO BROMODEOXYURIDINE. Exp Cell Res (1963) 11.23
The primary structure of rabbit beta-globin mRNA as determined from cloned DNA. Cell (1977) 11.05
Intragenic DNA spacers interrupt the ovalbumin gene. Proc Natl Acad Sci U S A (1978) 11.03
Synthesis of rabbit beta-globin in cultured monkey kidney cells following infection with a SV40 beta-globin recombinant genome. Nature (1979) 9.99
The transfer and stable integration of the HSV thymidine kinase gene into mouse cells. Cell (1978) 6.38
Removal of RNase activity from DNase by affinity chromatography on agarose coupled aminophenylphosphoryl-uridine-2' (3')-phosphate. Nucleic Acids Res (1977) 4.09
Rabbit beta-globin mRNA production in mouse L cells transformed with cloned rabbit beta-globin chromosomal DNA. Nature (1979) 3.88
Sodium pyrophosphate inhibition of RNA.DNA hybrid degradation by reverse transcriptase. Proc Natl Acad Sci U S A (1978) 3.43
Biochemical transformation of mouse cells by fragments of herpes simplex virus DNA. Cell (1977) 3.38
Analysis of the complexity and diversity of mRNA from chicken liver and oviduct. Cell (1976) 1.92
Characterization of the ribosome-protected regions of 125I-labelled rabbit globin messenger RNA. J Mol Biol (1976) 1.84
Mouse globin gene expression in erythroid and non-erythroid tissues. Cell (1976) 1.75
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Res (1984) 86.56
Nucleotide sequence of the rightward operator of phage lambda. Proc Natl Acad Sci U S A (1975) 56.75
The isolation of structural genes from libraries of eucaryotic DNA. Cell (1978) 37.13
The isolation and characterization of linked delta- and beta-globin genes from a cloned library of human DNA. Cell (1978) 22.88
The T4 gene encodes the AIDS virus receptor and is expressed in the immune system and the brain. Cell (1986) 22.26
Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis. Biochemistry (1975) 22.00
The structure and evolution of the human beta-globin gene family. Cell (1980) 21.24
DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells. Proc Natl Acad Sci U S A (1979) 21.22
Transformation of mammalian cells with genes from procaryotes and eucaryotes. Cell (1979) 21.15
Biochemical transfer of single-copy eucaryotic genes using total cellular DNA as donor. Cell (1978) 19.44
Transfer of purified herpes virus thymidine kinase gene to cultured mouse cells. Cell (1977) 18.95
A novel multigene family may encode odorant receptors: a molecular basis for odor recognition. Cell (1991) 18.19
Identification of two distinct regulatory regions adjacent to the human beta-interferon gene. Cell (1983) 17.60
Regulation of inducible and tissue-specific gene expression. Science (1987) 15.04
Amplification and characterization of a beta-globin gene synthesized in vitro. Cell (1976) 14.99
Functional homology of mammalian and yeast RAS genes. Cell (1985) 14.01
Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method. Mol Cell Biol (1988) 13.78
Analysis of transcriptional regulatory signals of the HSV thymidine kinase gene: identification of an upstream control region. Cell (1981) 12.73
Normal and mutant human beta-globin pre-mRNAs are faithfully and efficiently spliced in vitro. Cell (1984) 12.30
In yeast, RAS proteins are controlling elements of adenylate cyclase. Cell (1985) 11.63
The ubiquitin-proteasome pathway is required for processing the NF-kappa B1 precursor protein and the activation of NF-kappa B. Cell (1994) 11.62
The primary structure of rabbit beta-globin mRNA as determined from cloned DNA. Cell (1977) 11.05
Intragenic DNA spacers interrupt the ovalbumin gene. Proc Natl Acad Sci U S A (1978) 11.03
Detection and localization of single base changes by denaturing gradient gel electrophoresis. Methods Enzymol (1987) 10.90
In vivo footprinting of a muscle specific enhancer by ligation mediated PCR. Science (1989) 10.87
Cloning the differences between two complex genomes. Science (1993) 10.60
Identification of DNA sequences required for transcription of the human alpha 1-globin gene in a new SV40 host-vector system. Cell (1981) 9.81
Human beta-globin pre-mRNA synthesized in vitro is accurately spliced in Xenopus oocyte nuclei. Cell (1983) 9.77
Excision of an intact intron as a novel lariat structure during pre-mRNA splicing in vitro. Cell (1984) 9.13
The chromosomal arrangement of human alpha-like globin genes: sequence homology and alpha-globin gene deletions. Cell (1980) 9.13
Factor required for mammalian spliceosome assembly is localized to discrete regions in the nucleus. Nature (1990) 8.79
Visualizing an olfactory sensory map. Cell (1996) 8.79
Transcriptional activation: a complex puzzle with few easy pieces. Cell (1994) 8.63
The role of small nuclear ribonucleoprotein particles in pre-mRNA splicing. Nature (1987) 8.28
The nucleotide sequence of the human beta-globin gene. Cell (1980) 8.11
Specific interactions between proteins implicated in splice site selection and regulated alternative splicing. Cell (1993) 8.07
Molecular cloning and characterization of the human beta-like globin gene cluster. Cell (1980) 7.77
Genomic sequencing and methylation analysis by ligation mediated PCR. Science (1989) 7.38
Signal-induced site-specific phosphorylation targets I kappa B alpha to the ubiquitin-proteasome pathway. Genes Dev (1995) 7.36
NF-kappa B: a lesson in family values. Cell (1995) 7.33
Transcriptional activation of cloned human beta-globin genes by viral immediate-early gene products. Cell (1983) 7.22
Virus induction of human IFN beta gene expression requires the assembly of an enhanceosome. Cell (1995) 7.17
The isolation and nucleotide sequence of a cDNA encoding the T cell surface protein T4: a new member of the immunoglobulin gene family. Cell (1985) 7.16
Virus infection induces the assembly of coordinately activated transcription factors on the IFN-beta enhancer in vivo. Mol Cell (1998) 7.01
Genes in S. cerevisiae encoding proteins with domains homologous to the mammalian ras proteins. Cell (1984) 6.87
Multiple factors including the small nuclear ribonucleoproteins U1 and U2 are necessary for pre-mRNA splicing in vitro. Cell (1985) 6.85
Activation of the T24 bladder carcinoma transforming gene is linked to a single amino acid change. Nature (1982) 6.78
Genetic and physical linkage of exogenous sequences in transformed cells. Cell (1980) 6.67
Structure of the lambda operators. Nature (1973) 6.66
Genetic analysis of yeast RAS1 and RAS2 genes. Cell (1984) 6.60
Nearly all single base substitutions in DNA fragments joined to a GC-clamp can be detected by denaturing gradient gel electrophoresis. Nucleic Acids Res (1985) 6.52
Specific transcription and RNA splicing defects in five cloned beta-thalassaemia genes. Nature (1983) 6.48
A spatial map of olfactory receptor expression in the Drosophila antenna. Cell (1999) 6.42
A role for exon sequences and splice-site proximity in splice-site selection. Cell (1986) 6.41
A soluble form of CD4 (T4) protein inhibits AIDS virus infection. Nature (1988) 6.39
The transfer and stable integration of the HSV thymidine kinase gene into mouse cells. Cell (1978) 6.38
Fine structure genetic analysis of a beta-globin promoter. Science (1986) 6.36
Detection of single base substitutions by ribonuclease cleavage at mismatches in RNA:DNA duplexes. Science (1985) 6.35
Three different genes in S. cerevisiae encode the catalytic subunits of the cAMP-dependent protein kinase. Cell (1987) 6.35
Ordered recruitment of chromatin modifying and general transcription factors to the IFN-beta promoter. Cell (2000) 6.32
Sequence of a repressor-binding site in the DNA of bacteriophage lamda. Nature (1974) 6.22
Detection of single base substitutions in total genomic DNA. Nature (1985) 5.99
Allelic inactivation regulates olfactory receptor gene expression. Cell (1994) 5.93
Site-specific phosphorylation of IkappaBalpha by a novel ubiquitination-dependent protein kinase activity. Cell (1996) 5.91
The high mobility group protein HMG I(Y) is required for NF-kappa B-dependent virus induction of the human IFN-beta gene. Cell (1992) 5.85
A novel ras-related gene family. Cell (1985) 5.81
The human beta-interferon gene enhancer is under negative control. Cell (1986) 5.61
Transcriptional regulation of endothelial cell adhesion molecules: NF-kappa B and cytokine-inducible enhancers. FASEB J (1995) 5.59
The involvement of NF-kappa B in beta-interferon gene regulation reveals its role as widely inducible mediator of signal transduction. Cell (1989) 5.50
Identification of immediate early genes from herpes simplex virus that transactivate the virus thymidine kinase gene. Proc Natl Acad Sci U S A (1985) 5.40
Enzymatic in vitro synthesis of globin genes. Cell (1976) 5.31
Intron sequences involved in lariat formation during pre-mRNA splicing. Cell (1985) 5.30
An olfactory sensory map in the fly brain. Cell (2000) 5.29
Selective digestion of transcriptionally active ovalbumin genes from oviduct nuclei. Proc Natl Acad Sci U S A (1976) 5.27
Structure and activation of the human N-ras gene. Cell (1983) 5.25
Multiple repressor binding at the operators in bacteriophage lambda. Proc Natl Acad Sci U S A (1973) 5.15
Differences in human alpha- and beta-globin gene expression in mouse erythroleukemia cells: the role of intragenic sequences. Cell (1984) 5.12
Cloning and characterization of BCY1, a locus encoding a regulatory subunit of the cyclic AMP-dependent protein kinase in Saccharomyces cerevisiae. Mol Cell Biol (1987) 5.03
A novel family of genes encoding putative pheromone receptors in mammals. Cell (1995) 4.88
A splicing enhancer complex controls alternative splicing of doublesex pre-mRNA. Cell (1993) 4.82
Crystal structure of an HIV-binding recombinant fragment of human CD4. Nature (1990) 4.81
DNA sequence and characterization of the S. cerevisiae gene encoding adenylate cyclase. Cell (1985) 4.80
The S. cerevisiae CDC25 gene product regulates the RAS/adenylate cyclase pathway. Cell (1987) 4.74
Topographic organization of sensory projections to the olfactory bulb. Cell (1994) 4.74
NF-kappaB signaling pathways in mammalian and insect innate immunity. Genes Dev (2001) 4.70