Published in Proc Natl Acad Sci U S A on March 01, 1986
A functional classification scheme for beta-lactamases and its correlation with molecular structure. Antimicrob Agents Chemother (1995) 26.51
The active-site-serine penicillin-recognizing enzymes as members of the Streptomyces R61 DD-peptidase family. Biochem J (1988) 7.51
Characterization of beta-lactamases. Antimicrob Agents Chemother (1989) 5.13
The diversity of the catalytic properties of class A beta-lactamases. Biochem J (1990) 3.98
Role of the conserved amino acids of the 'SDN' loop (Ser130, Asp131 and Asn132) in a class A beta-lactamase studied by site-directed mutagenesis. Biochem J (1990) 2.27
Beta-lactamases as fully efficient enzymes. Determination of all the rate constants in the acyl-enzyme mechanism. Biochem J (1990) 1.75
Protein homeostasis in models of aging and age-related conformational disease. Adv Exp Med Biol (2010) 1.48
A comprehensive, high-resolution map of a gene's fitness landscape. Mol Biol Evol (2014) 1.36
Identification of a structural determinant for resistance to beta-lactam antibiotics in Gram-positive bacteria. Proc Natl Acad Sci U S A (1998) 1.27
Gene synthesis, expression, and mutagenesis of the blue copper proteins azurin and plastocyanin. Proc Natl Acad Sci U S A (1991) 1.13
Site-directed mutagenesis of dicarboxylic acids near the active site of Bacillus cereus 5/B/6 beta-lactamase II. Biochem J (1991) 1.12
Extension of the substrate spectrum by an amino acid substitution at residue 219 in the Citrobacter freundii cephalosporinase. J Bacteriol (1990) 1.09
A method for introducing random single point deletions in specific DNA target sequences using oligonucleotides. Nucleic Acids Res (1989) 1.05
The synthesis of blocked triplet-phosphoramidites and their use in mutagenesis. Nucleic Acids Res (1995) 1.01
Missense mutations and evolutionary conservation of amino acids: evidence that many of the amino acids in factor IX function as "spacer" elements. Am J Hum Genet (1991) 0.96
Altering enzymatic activity: recruitment of carboxypeptidase activity into an RTEM beta-lactamase/penicillin-binding protein 5 chimera. Proc Natl Acad Sci U S A (1990) 0.89
Recovery of active beta-lactamases from Proteus vulgaris and RTEM-1 hybrid by random mutagenesis by using a dnaQ strain of Escherichia coli. Antimicrob Agents Chemother (1996) 0.84
Determinants of EcoRI endonuclease sequence discrimination. Proc Natl Acad Sci U S A (1989) 0.82
Structure-based design of combinatorial mutagenesis libraries. Protein Sci (2015) 0.80
Point mutations of two arginine residues in the Streptomyces R61 DD-peptidase. Biochem J (1992) 0.75
Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol (1980) 287.68
Studies on transformation of Escherichia coli with plasmids. J Mol Biol (1983) 116.81
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene (1977) 114.87
New M13 vectors for cloning. Methods Enzymol (1983) 90.74
A complementation analysis of the restriction and modification of DNA in Escherichia coli. J Mol Biol (1969) 71.21
A system for shotgun DNA sequencing. Nucleic Acids Res (1981) 52.99
Rapid and efficient cosmid cloning. Nucleic Acids Res (1981) 40.13
Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors. Methods Enzymol (1983) 17.47
The generation of a ColE1-Apr cloning vehicle which allows detection of inserted DNA. Mol Gen Genet (1975) 8.80
Biological properties of human c-Ha-ras1 genes mutated at codon 12. Nature (1984) 6.21
Analytical isoelectric focusing of R factor-determined beta-lactamases: correlation with plasmid compatibility. J Bacteriol (1976) 5.07
Oligonucleotide-directed mutagenesis as a general and powerful method for studies of protein function. Proc Natl Acad Sci U S A (1982) 4.64
beta-Lactamase proceeds via an acyl-enzyme intermediate. Interaction of the Escherichia coli RTEM enzyme with cefoxitin. Biochemistry (1980) 3.94
Redesigning trypsin: alteration of substrate specificity. Science (1985) 3.70
Efficient site-directed mutagenesis by simultaneous use of two primers. Nucleic Acids Res (1983) 2.73
The role of the beta-lactamase signal sequence in the secretion of proteins by Escherichia coli. J Biol Chem (1984) 2.72
Redesigning enzyme structure by site-directed mutagenesis: tyrosyl tRNA synthetase and ATP binding. Nature (1982) 2.63
Two-codon insertion mutagenesis of plasmid genes by using single-stranded hexameric oligonucleotides. Proc Natl Acad Sci U S A (1985) 2.50
Penicillinase active sites: labelling of serine-44 in beta-lactamase I by 6beta-bromopenicillanic acid. FEBS Lett (1979) 2.45
Cassette mutagenesis: an efficient method for generation of multiple mutations at defined sites. Gene (1985) 2.15
Directed mutagenesis of dihydrofolate reductase. Science (1983) 1.88
Disulfide bond engineered into T4 lysozyme: stabilization of the protein toward thermal inactivation. Science (1984) 1.81
Effect of single amino acid replacements on the thermal stability of the NH2-terminal domain of phage lambda repressor. Proc Natl Acad Sci U S A (1984) 1.80
A large increase in enzyme-substrate affinity by protein engineering. Nature (1984) 1.46
Active site of triosephosphate isomerase: in vitro mutagenesis and characterization of an altered enzyme. Proc Natl Acad Sci U S A (1985) 1.44
Effects of site-specific amino acid modification on protein interactions and biological function. Annu Rev Biochem (1985) 1.42
Site-specific mutagenesis of aspartate transcarbamoylase. Replacement of tyrosine 165 in the catalytic chain by serine reduces enzymatic activity. J Biol Chem (1984) 1.06
Probing the catalytic mechanism of yeast triose phosphate isomerase by site-specific mutagenesis. Biochem Soc Trans (1984) 0.94
Determination of intracellular pH by 31P magnetic resonance. J Biol Chem (1973) 6.71
Molecular basis of double-stranded RNA-protein interactions: structure of a dsRNA-binding domain complexed with dsRNA. EMBO J (1998) 4.87
Oligonucleotide-directed mutagenesis as a general and powerful method for studies of protein function. Proc Natl Acad Sci U S A (1982) 4.64
Fragments of the HIV-1 Tat protein specifically bind TAR RNA. Science (1990) 4.33
Structure of the RNA-dependent RNA polymerase of poliovirus. Structure (1997) 3.88
Crystal structure of the Oxytricha nova telomere end binding protein complexed with single strand DNA. Cell (1998) 3.03
A chemically synthesized pre-sequence of an imported mitochondrial protein can form an amphiphilic helix and perturb natural and artificial phospholipid bilayers. EMBO J (1986) 2.91
Soluble transition metals mediate residual oil fly ash induced acute lung injury. J Toxicol Environ Health (1997) 2.76
Genetics of drought adaptation in Arabidopsis thaliana: I. Pleiotropy contributes to genetic correlations among ecological traits. Mol Ecol (2003) 2.68
Oligomeric structures of poliovirus polymerase are important for function. EMBO J (2001) 2.27
Modeling the cAMP-induced allosteric transition using the crystal structure of CAP-cAMP at 2.1 A resolution. J Mol Biol (2000) 2.23
Functional oligomerization of poliovirus RNA-dependent RNA polymerase. RNA (1995) 2.05
Amphiphilicity is essential for mitochondrial presequence function. EMBO J (1988) 1.93
Electron tunneling in proteins: coupling through a beta strand. Science (1995) 1.79
Carbon nuclear magnetic resonance studies of the histidine residue in alpha-lytic protease. Implications for the catalytic mechanism of serine proteases. Biochemistry (1973) 1.77
DNA G-quartets in a 1.86 A resolution structure of an Oxytricha nova telomeric protein-DNA complex. J Mol Biol (2001) 1.58
Mechanism of action of serine proteases: tetrahedral intermediate and concerted proton transfer. Biochemistry (1976) 1.41
Properties of photogenerated tryptophan and tyrosyl radicals in structurally characterized proteins containing rhenium(I) tricarbonyl diimines. J Am Chem Soc (2001) 1.37
Substrate specificities in class A beta-lactamases: preference for penams vs. cephems. The role of residue 237. Proteins (1989) 1.36
Sequence-specific recognition of DNA by zinc-finger peptides derived from the transcription factor Sp1. Proc Natl Acad Sci U S A (1992) 1.35
Modulation of telomerase activity by telomere DNA-binding proteins in Oxytricha. Genes Dev (1998) 1.33
Night-time conductance in C3 and C4 species: do plants lose water at night? J Exp Bot (2003) 1.33
Stability of wild-type and mutant RTEM-1 beta-lactamases: effect of the disulfide bond. Proteins (1987) 1.28
Active-site mutants of beta-lactamase: use of an inactive double mutant to study requirements for catalysis. Biochemistry (1986) 1.25
Construction and characterization of an azurin analog for the purple copper site in cytochrome c oxidase. Proc Natl Acad Sci U S A (1996) 1.22
Extensive summer water pulses do not necessarily lead to canopy growth of Great Basin and northern Mojave Desert shrubs. Oecologia (2003) 1.21
Influence of temporal heterogeneity in nitrogen supply on competitive interactions in a desert shrub community. Oecologia (2007) 1.21
Phosphorus in periphyton mats provides the best metric for detecting low-level P enrichment in an oligotrophic wetland. Water Res (2004) 1.19
Importance of internal hydraulic redistribution for prolonging the lifespan of roots in dry soil. Plant Cell Environ (2007) 1.14
Does seed dispersal limit initiation of primary succession in desert playas? Am J Bot (1998) 1.14
Crystal structure of a model branchpoint-U2 snRNA duplex containing bulged adenosines. RNA (2001) 1.14
Gene synthesis, expression, and mutagenesis of the blue copper proteins azurin and plastocyanin. Proc Natl Acad Sci U S A (1991) 1.13
Crystal structure of the N-terminal domain of Oxytricha nova telomere end-binding protein alpha subunit both uncomplexed and complexed with telomeric ssDNA. J Mol Biol (2001) 1.10
Role of soluble metals in oil fly ash-induced airway epithelial injury and cytokine gene expression. Am J Physiol (1999) 1.08
Irrigation and fertilization effects on seed number, size, germination and seedling growth: implications for desert shrub establishment. Oecologia (2008) 1.08
Effect of ozone on diesel exhaust particle toxicity in rat lung. Toxicol Appl Pharmacol (2000) 1.07
Epithelial injury induced by exposure to residual oil fly-ash particles: role of reactive oxygen species? Am J Respir Cell Mol Biol (1997) 1.05
Water-soluble, recombinant CuA-domain of the cytochrome ba3 subunit II from Thermus thermophilus. Biochemistry (1996) 1.05
Creation of a test plasmid for detecting G-C-to-T-A transversions by changing serine to arginine in the active site of beta-lactamase. J Bacteriol (1987) 1.03
Nitrogen addition increases fecundity in the desert shrub Sarcobatus vermiculatus. Oecologia (2005) 1.02
Local adaptation across a climatic gradient despite small effective population size in the rare sapphire rockcress. Proc Biol Sci (2001) 1.01
Catalytic mechanism of serine proteases: reexamination of the pH dependence of the histidyl 1J13C2-H coupling constant in the catalytic triad of alpha-lytic protease. Proc Natl Acad Sci U S A (1981) 1.01
Structurally engineered cytochromes with unusual ligand-binding properties: expression of Saccharomyces cerevisiae Met-80-->Ala iso-1-cytochrome c. Proc Natl Acad Sci U S A (1993) 1.00
Disruption of normal iron homeostasis after bronchial instillation of an iron-containing particle. Am J Physiol (1998) 1.00
Mechanism of action of adenosylcobalamin: glycerol and other substrate analogues as substrates and inactivators for propanediol dehydratase--kinetics, stereospecificity, and mechanism. Biochemistry (1977) 1.00
High apoplastic solute concentrations in leaves alter water relations of the halophytic shrub, Sarcobatus vermiculatus. J Exp Bot (2005) 0.96
Kinetic and thermodynamic consequences of the removal of the Cys-77-Cys-123 disulphide bond for the folding of TEM-1 beta-lactamase. Biochem J (1997) 0.96
The beta-adrenergic receptor in the human neutrophil plasma membrane: receptor-cyclase uncoupling is associated with amplified GTP activation. J Immunol (1984) 0.93
Enzyme--substrate interaction by nuclear magnetic resonance. J Am Chem Soc (1967) 0.93
Identification of receptor regulatory proteins, membrane glycoproteins, and functional characteristics of adenylate cyclase in vesicles derived from the human neutrophil. Mol Immunol (1984) 0.91
Residual oil fly ash induces cytotoxicity and mucin secretion by guinea pig tracheal epithelial cells via an oxidant-mediated mechanism. Toxicol Appl Pharmacol (2000) 0.91
Competition for phosphorus: differential uptake from dual-isotope--labeled soil interspaces between shrub and grass. Science (1985) 0.89
Mechamism of action of coenzyme B12. Hydrogen transfer in the isomerization of methylmalonyl coenzyme A to succinyl coenzyme A. J Am Chem Soc (1969) 0.89
Altering enzymatic activity: recruitment of carboxypeptidase activity into an RTEM beta-lactamase/penicillin-binding protein 5 chimera. Proc Natl Acad Sci U S A (1990) 0.89
Purification and subunit characterization of propanediol dehydratase, a membrane-associated enzyme. Biochemistry (1981) 0.87
Magnetic resonance studies of the binding site interactions between 19F-labeled nitrophenyl haptens and specific mouse myeloma immunoglobulin MOPC-315. Biochemistry (1978) 0.87
Spectroscopic studies of the nature of ligand bonding in carbonmonoxyhemoglobins: evidence of a specific function for histidine-E7 from infrared and nuclear magnetic resonance intensities. Biochemistry (1978) 0.86
Mechanism of action of coenzyme B 12 . Hydrogen transfer in the isomerization of -methylaspartate to glutamate. Biochemistry (1972) 0.85
Plant N capture from pulses: effects of pulse size, growth rate, and other soil resources. Oecologia (2005) 0.85
Studies on the variation in the viscosity of bronchial mucus and a technique for reducing such variation. Biorheology (1974) 0.85
Iron disequilibrium in the rat lung after instilled blood. Chest (2000) 0.85
Metal-dependent expression of ferritin and lactoferrin by respiratory epithelial cells. Am J Physiol (1998) 0.85
Magnetic resonance studies of the binding of 13C-labeled carbon monoxide to myoglobins and hemoglobins containing modified hemes. Biochemistry (1977) 0.84
Cytokine mediation of ozone-induced pulmonary adaptation. Am J Respir Cell Mol Biol (1998) 0.84
Studies on C3ahu binding to human eosinophils: characterization of binding. Int Arch Allergy Appl Immunol (1984) 0.84
Adaptation to ozone in rats and its association with ascorbic acid in the lung. Fundam Appl Toxicol (1996) 0.83
Pulsed EPR/ENDOR characterization of perturbations of the Cu(A) center ground state by axial methionine ligand mutations. J Am Chem Soc (2001) 0.83
Diffuse alveolar damage after exposure to an oil fly ash. Am J Respir Crit Care Med (2001) 0.83
Diol dehydratase: N-terminal amino acid sequences and subunit stoichiometry. Biochem Biophys Res Commun (1982) 0.83
Magnetic resonance studies of the binding site interactions between phosphorylcholine and specific mouse myeloma immunoglobulin. Biochemistry (1977) 0.83
A totally synthetic histidine-2 ferredoxin: thermal stability and redox properties. Biochemistry (1991) 0.83
Ozone effects on airway responsiveness, lung injury, and inflammation. Comparative rat strain and in vivo/in vitro investigations. Inhal Toxicol (1999) 0.83
Molecular studies of subspecificity differences among phosphorylcholine-binding mouse myeloma antibodies using 31P nuclear magnetic resonance. Biochemistry (1978) 0.83
Diminished injury in hypotransferrinemic mice after exposure to a metal-rich particle. Am J Physiol Lung Cell Mol Physiol (2000) 0.82
Ferritin expression after in vitro exposures of human alveolar macrophages to silica is iron-dependent. Am J Respir Cell Mol Biol (1997) 0.82
Intra-specific variation in xylem cavitation in interior live oak (Quercus wislizenii A. DC.). J Exp Bot (2001) 0.82
Ozone toxicity in the rat. III. Effect of changes in ambient temperature on pulmonary parameters. J Appl Physiol (1985) (1996) 0.82
Ionization behavior of the histidine residue in the catalytic triad of serine proteases. Mechanistic implications. J Biol Chem (1973) 0.82
Mechanism of action of adenosylcobalamin: hydrogen transfer in the inactivation of diol dehydratase by glycerol. Biochemistry (1978) 0.82
Daily goals, life goals, and worst fears: means, ends, and subjective well-being. J Pers (1998) 0.81
Metal storage and transport proteins increase after exposure of the rat lung to an air pollution particle. Toxicol Pathol (1998) 0.81
Nuclear magnetic resonance studies of 13 CO binding to various heme globins. J Am Chem Soc (1972) 0.81
Sarcoidosis manifesting as acute appendicitis: report of a case. Dis Colon Rectum (1997) 0.81
Subcutaneous oxygen tension: a useful adjunct in assessment of perfusion status. Crit Care Med (1995) 0.80
Conformation studies of various hemoglobins by natural-abundance 13 C NMR spectroscopy. Proc Natl Acad Sci U S A (1972) 0.80
Ozone adaptation in mice and its association with ascorbic acid in the lung. Inhal Toxicol (2000) 0.80
Site saturation of the histidine-46 position in Pseudomonas aeruginosa azurin: characterization of the His46Asp copper and cobalt proteins. Biochemistry (1993) 0.80
Accumulation of iron in the rat lung after tracheal instillation of diesel particles. Toxicol Pathol (2001) 0.80
Lack of DNA adduct formation in mice treated with benzene. Mutat Res (1994) 0.80
Diaspirin crosslinked hemoglobin (DCLHb): more effective than lactated Ringer's solution in restoring central venous oxygen saturation after hemorrhagic shock in rats. Artif Cells Blood Substit Immobil Biotechnol (1996) 0.79
Magnetic resonance studies of protein-small molecule interactions. Binding of N-trifluoroacetyl-D-(and L-)-p-fluorophenylalanine to -chymotrypsin. J Am Chem Soc (1972) 0.79
13C NMR studies of the binding of soybean trypsin inhibitor to trypsin. Biochem Biophys Res Commun (1979) 0.79
Depletion of iron and ascorbate in rodents diminishes lung injury after silica. Exp Lung Res (1998) 0.79
Prevention of aggregation of synthetic membrane-spanning peptides by addition of detergent. Anal Biochem (1988) 0.79
Studies on the catalytic mechanism of pepsin using a new synthetic substrate. Biochemistry (1972) 0.79
Stereospecificity and mechanism of adenosylcobalamin-dependent diol dehydratase. Catalysis and inactivation with meso- and dl-2,3-butanediols as substrates. Biochem Biophys Res Commun (1979) 0.79
Transcriptional activation of inflammasome components by Libby amphibole and the role of iron. Inhal Toxicol (2011) 0.79
Magnetic resonance studies of protein-small molecule interactions. Dynamics of binding between N-trifluoroacetyl-D-tryptophan and -chymotrypsin. J Am Chem Soc (1972) 0.78
A comparative study of the effects of inhaled cadmium chloride and cadmium oxide: pulmonary response. J Toxicol Environ Health (1987) 0.77