Published in Proc Natl Acad Sci U S A on July 01, 1982
High level transient expression of a chloramphenicol acetyl transferase gene by DEAE-dextran mediated DNA transfection coupled with a dimethyl sulfoxide or glycerol shock treatment. Nucleic Acids Res (1984) 10.40
Transformation and replication in mouse cells of a bovine papillomavirus--pML2 plasmid vector that can be rescued in bacteria. Proc Natl Acad Sci U S A (1982) 5.22
Immunoglobulin gene expression in transformed lymphoid cells. Proc Natl Acad Sci U S A (1983) 5.18
Localization and analysis of bovine papillomavirus type 1 transforming functions. J Virol (1984) 4.77
Bovine papilloma virus contains an activator of gene expression at the distal end of the early transcription unit. Mol Cell Biol (1983) 4.56
cis and trans activation of globin gene transcription in transient assays. Proc Natl Acad Sci U S A (1983) 4.41
Rearrangement and mutagenesis of a shuttle vector plasmid after passage in mammalian cells. Proc Natl Acad Sci U S A (1983) 3.90
Translation of open reading frame E5 of bovine papillomavirus is required for its transforming activity. Proc Natl Acad Sci U S A (1986) 3.17
A cosmid for selecting genes by complementation in Aspergillus nidulans: Selection of the developmentally regulated yA locus. Proc Natl Acad Sci U S A (1985) 3.04
Human papillomavirus type 16 DNA-induced malignant transformation of NIH 3T3 cells. J Virol (1986) 3.03
Transcriptional organization of bovine papillomavirus type 1. J Virol (1983) 2.84
Adenovirus DNA replication in vitro: synthesis of full-length DNA with purified proteins. Proc Natl Acad Sci U S A (1983) 2.58
44-amino-acid E5 transforming protein of bovine papillomavirus requires a hydrophobic core and specific carboxyl-terminal amino acids. Mol Cell Biol (1988) 2.50
Transient replication of bovine papilloma virus type 1 plasmids: cis and trans requirements. Proc Natl Acad Sci U S A (1986) 2.49
A bovine papillomavirus E1-related protein binds specifically to bovine papillomavirus DNA. J Virol (1991) 2.43
Origin of replication in episomal bovine papilloma virus type 1 DNA isolated from transformed cells. EMBO J (1984) 2.26
Expression and regulation of chicken actin genes introduced into mouse myogenic and nonmyogenic cells. Proc Natl Acad Sci U S A (1984) 2.26
Genetic and biochemical definition of the bovine papillomavirus E5 transforming protein. EMBO J (1987) 2.23
Nonsense mutation in open reading frame E2 of bovine papillomavirus DNA. J Virol (1986) 2.22
A stable bovine papillomavirus hybrid plasmid that expresses a dominant selective trait. Mol Cell Biol (1983) 2.21
Versatile cosmid vectors for the isolation, expression, and rescue of gene sequences: studies with the human alpha-globin gene cluster. Proc Natl Acad Sci U S A (1983) 2.14
Bovine papillomavirus mutant temperature sensitive for transformation, replication and transactivation. EMBO J (1988) 1.89
The AU-rich sequences in the 3' untranslated region mediate the increased turnover of interferon mRNA induced by glucocorticoids. J Exp Med (1991) 1.84
Regulated expression of an extrachromosomal human beta-interferon gene in mouse cells. Proc Natl Acad Sci U S A (1982) 1.80
Error-prone mutagenesis detected in mammalian cells by a shuttle vector containing the supF gene of Escherichia coli. Mol Cell Biol (1984) 1.76
Cell-specified expression of a selectable hybrid gene. Proc Natl Acad Sci U S A (1984) 1.74
Conserved chromosomal positions of dual domains of the ets protooncogene in cats, mice, and humans. Proc Natl Acad Sci U S A (1986) 1.67
Lines of BPV-transformed murine cells that constitutively express influenza virus hemagglutinin. EMBO J (1985) 1.66
Transforming activity of a 16-amino-acid segment of the bovine papillomavirus E5 protein linked to random sequences of hydrophobic amino acids. J Virol (1989) 1.54
Expression and regulation of a human metallothionein gene carried on an autonomously replicating shuttle vector. Proc Natl Acad Sci U S A (1983) 1.54
SV40 T antigen and the exocytotic pathway. EMBO J (1985) 1.52
Expression of minute virus of mice structural proteins in murine cell lines transformed by bovine papillomavirus-minute virus of mice plasmid chimera. J Virol (1984) 1.51
Interferon induces morphologic reversion with elimination of extrachromosomal viral genomes in bovine papillomavirus-transformed mouse cells. Proc Natl Acad Sci U S A (1982) 1.46
Direct isolation of the functional human thymidine kinase gene with a cosmid shuttle vector. Proc Natl Acad Sci U S A (1984) 1.45
Bovine papillomavirus E2 gene regulates expression of the viral E5 transforming gene. J Virol (1988) 1.40
Expression of cloned immunoglobulin genes introduced into mouse L cells. Nucleic Acids Res (1983) 1.36
A bovine papilloma virus vector with a dominant resistance marker replicates extrachromosomally in mouse and E. coli cells. EMBO J (1983) 1.33
Inducible expression of the human interferon beta 1 gene linked to a bovine papilloma virus DNA vector and maintained extrachromosomally in mouse cells. Mol Cell Biol (1983) 1.28
Evidence that replication initiates at only some of the potential origins in each oligomeric form of bovine papillomavirus type 1 DNA. Mol Cell Biol (1990) 1.28
Enhancer-dependent expression of the rat preproinsulin gene in bovine papillomavirus type 1 vectors. Mol Cell Biol (1985) 1.25
Expression of intron-containing C. elegans heat shock genes in mouse cells demonstrates divergence of 3' splice site recognition sequences between nematodes and vertebrates, and an inhibitory effect of heat shock on the mammalian splicing apparatus. Nucleic Acids Res (1987) 1.22
Efficient transcription of a Caenorhabditis elegans heat shock gene pair in mouse fibroblasts is dependent on multiple promoter elements which can function bidirectionally. Mol Cell Biol (1986) 1.20
Mutational analysis of open reading frame E4 of bovine papillomavirus type 1. J Virol (1987) 1.17
The bovine papillomavirus distal "enhancer" is not cis essential for transformation or for plasmid maintenance. Mol Cell Biol (1985) 1.14
Expression of the Epstein-Barr virus gp350/220 gene in rodent and primate cells. J Virol (1987) 1.13
Enhanced production of hepatitis B surface antigen in NIH 3T3 mouse fibroblasts by using extrachromosomally replicating bovine papillomavirus vector. Mol Cell Biol (1983) 1.09
BK virus-plasmid expression vector that persists episomally in human cells and shuttles into Escherichia coli. Mol Cell Biol (1984) 1.06
Fate of exogenous recombinant plasmids introduced into mouse and human cells. Nucleic Acids Res (1985) 1.06
High-level expression of a cloned HLA heavy chain gene introduced into mouse cells on a bovine papillomavirus vector. Mol Cell Biol (1984) 0.99
Hierarchy of polyadenylation site usage by bovine papillomavirus in transformed mouse cells. J Virol (1993) 0.95
Plasmidial maintenance in rodent fibroblasts of a BPV1-pBR322 shuttle vector without immediately apparent oncogenic transformation of the recipient cells. EMBO J (1984) 0.93
Characterization of a retrovirus shuttle vector capable of either proviral integration or extrachromosomal replication in mouse cells. Mol Cell Biol (1985) 0.87
The molecular biology of papillomavirus transformation. Warner-Lambert Parke-Davis Award Lecture. Am J Pathol (1983) 0.86
High recombination rate of an Epstein-Barr virus-simian virus 40 hybrid shuttle vector in human cells. J Virol (1989) 0.85
Analysis of nonpermissivity in mouse cells overexpressing simian virus 40 T antigen. J Virol (1987) 0.83
Plasmidal maintenance of composite DNA derived from polyoma related plasmid, L factor. Nucleic Acids Res (1987) 0.81
Expression of a human U1 RNA gene introduced into mouse cells via bovine papillomavirus DNA vectors. Mol Cell Biol (1985) 0.81
The bovine papillomavirus genome and its uses as a eukaryotic vector. Biochem J (1987) 0.80
In vitro methylation of bovine papillomavirus alters its ability to transform mouse cells. Mol Cell Biol (1986) 0.79
Posttranscriptional regulation of beta interferon expression in erythroid Friend cells treated with gamma interferon. J Virol (1991) 0.75
Recent advances in preclinical model systems for papillomaviruses. Virus Res (2016) 0.75
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Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol (1967) 106.91
Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A (1979) 72.18
Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids. Cell (1977) 61.24
A rapid boiling method for the preparation of bacterial plasmids. Anal Biochem (1981) 59.01
Mapping of RNA by a modification of the Berk-Sharp procedure: the 5' termini of 15 S beta-globin mRNA precursor and mature 10 s beta-globin mRNA have identical map coordinates. Nucleic Acids Res (1979) 31.23
Transcription maps of polyoma virus-specific RNA: analysis by two-dimensional nuclease S1 gel mapping. Methods Enzymol (1980) 24.43
Transformation of mammalian cells with genes from procaryotes and eucaryotes. Cell (1979) 21.15
Expression of a beta-globin gene is enhanced by remote SV40 DNA sequences. Cell (1981) 20.72
Inhibition of SV40 replication in simian cells by specific pBR322 DNA sequences. Nature (1981) 13.89
Synthesis of rabbit beta-globin in cultured monkey kidney cells following infection with a SV40 beta-globin recombinant genome. Nature (1979) 9.99
Identification of DNA sequences required for transcription of the human alpha 1-globin gene in a new SV40 host-vector system. Cell (1981) 9.81
Mouse cells transformed by bovine papillomavirus contain only extrachromosomal viral DNA sequences. Proc Natl Acad Sci U S A (1981) 9.27
Isolation and characterisation of a yeast chromosomal replicator. Nature (1979) 9.14
The nucleotide sequence of the human beta-globin gene. Cell (1980) 8.11
Molecular cloning and characterization of the human beta-like globin gene cluster. Cell (1980) 7.77
A small segment of polyoma virus DNA enhances the expression of a cloned beta-globin gene over a distance of 1400 base pairs. Nucleic Acids Res (1981) 7.57
Biochemical method for mapping mutational alterations in DNA with S1 nuclease: the location of deletions and temperature-sensitive mutations in simian virus 40. Proc Natl Acad Sci U S A (1975) 7.57
Isolation of mutants of an animal virus in bacteria. Science (1980) 6.81
In vitro tumorigenic transformation by a defined sub-genomic fragment of bovine papilloma virus DNA. Nature (1980) 6.67
Genetic and physical linkage of exogenous sequences in transformed cells. Cell (1980) 6.67
Bovine papilloma virus deoxyribonucleic acid: a novel eucaryotic cloning vector. Mol Cell Biol (1981) 4.75
Isolation of the chicken thymidine kinase gene by plasmid rescue. Nature (1980) 4.37
A new method for the purification and identification of covalently closed circular DNA molcules. Nucleic Acids Res (1978) 4.09
Characteristics of an SV40-plasmid recombinant and its movement into and out of the genome of a murine cell. Cell (1980) 3.66
Mechanisms and applications of DNA-mediated gene transfer in mammalian cells - a review. Gene (1981) 3.46
Structure and expression of a cloned beta o thalassaemic globin gene. Nucleic Acids Res (1981) 2.71
Complete nucleotide sequence of the 5' noncoding region of human alpha-and beta-globin mRNA. Cell (1977) 2.62
Identification of a BALB/c H-2Ld gene by DNA-mediated gene transfer. Science (1982) 1.72
Recovery of recombinant bacterial plasmids from E. coli transformed with DNA from microinjected mouse cells. Nucleic Acids Res (1981) 1.39
Structure of the human G gamma-A gamma-delta-beta-globin gene locus. Proc Natl Acad Sci U S A (1979) 1.25
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Res (1984) 86.56
Nucleotide sequence of the rightward operator of phage lambda. Proc Natl Acad Sci U S A (1975) 56.75
The isolation of structural genes from libraries of eucaryotic DNA. Cell (1978) 37.13
Transcription maps of polyoma virus-specific RNA: analysis by two-dimensional nuclease S1 gel mapping. Methods Enzymol (1980) 24.43
The isolation and characterization of linked delta- and beta-globin genes from a cloned library of human DNA. Cell (1978) 22.88
Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis. Biochemistry (1975) 22.00
The structure and evolution of the human beta-globin gene family. Cell (1980) 21.24
Transformation of mammalian cells with genes from procaryotes and eucaryotes. Cell (1979) 21.15
Identification of two distinct regulatory regions adjacent to the human beta-interferon gene. Cell (1983) 17.60
Regulation of inducible and tissue-specific gene expression. Science (1987) 15.04
Amplification and characterization of a beta-globin gene synthesized in vitro. Cell (1976) 14.99
Normal and mutant human beta-globin pre-mRNAs are faithfully and efficiently spliced in vitro. Cell (1984) 12.30
The ubiquitin-proteasome pathway is required for processing the NF-kappa B1 precursor protein and the activation of NF-kappa B. Cell (1994) 11.62
The primary structure of rabbit beta-globin mRNA as determined from cloned DNA. Cell (1977) 11.05
Detection and localization of single base changes by denaturing gradient gel electrophoresis. Methods Enzymol (1987) 10.90
Transient accumulation of c-fos RNA following serum stimulation requires a conserved 5' element and c-fos 3' sequences. Cell (1985) 10.00
Identification of DNA sequences required for transcription of the human alpha 1-globin gene in a new SV40 host-vector system. Cell (1981) 9.81
Human beta-globin pre-mRNA synthesized in vitro is accurately spliced in Xenopus oocyte nuclei. Cell (1983) 9.77
Isolation and properties of cDNA clones encoding SRF, a transcription factor that binds to the c-fos serum response element. Cell (1988) 9.34
Excision of an intact intron as a novel lariat structure during pre-mRNA splicing in vitro. Cell (1984) 9.13
The chromosomal arrangement of human alpha-like globin genes: sequence homology and alpha-globin gene deletions. Cell (1980) 9.13
Identification of a protein-binding site that mediates transcriptional response of the c-fos gene to serum factors. Cell (1986) 9.10
Factor required for mammalian spliceosome assembly is localized to discrete regions in the nucleus. Nature (1990) 8.79
Transcriptional activation: a complex puzzle with few easy pieces. Cell (1994) 8.63
The role of small nuclear ribonucleoprotein particles in pre-mRNA splicing. Nature (1987) 8.28
The Rho family GTPases RhoA, Rac1, and CDC42Hs regulate transcriptional activation by SRF. Cell (1995) 8.20
The nucleotide sequence of the human beta-globin gene. Cell (1980) 8.11
Specific interactions between proteins implicated in splice site selection and regulated alternative splicing. Cell (1993) 8.07
Molecular cloning and characterization of the human beta-like globin gene cluster. Cell (1980) 7.77
The SRF accessory protein Elk-1 contains a growth factor-regulated transcriptional activation domain. Cell (1993) 7.37
Signal-induced site-specific phosphorylation targets I kappa B alpha to the ubiquitin-proteasome pathway. Genes Dev (1995) 7.36
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Transcriptional activation of cloned human beta-globin genes by viral immediate-early gene products. Cell (1983) 7.22
Virus induction of human IFN beta gene expression requires the assembly of an enhanceosome. Cell (1995) 7.17
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Transformation of rat cells by an altered polyoma virus genome expressing only the middle-T protein. Nature (1981) 6.70
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Nearly all single base substitutions in DNA fragments joined to a GC-clamp can be detected by denaturing gradient gel electrophoresis. Nucleic Acids Res (1985) 6.52
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A role for exon sequences and splice-site proximity in splice-site selection. Cell (1986) 6.41
Fine structure genetic analysis of a beta-globin promoter. Science (1986) 6.36
Removal of poly(A) and consequent degradation of c-fos mRNA facilitated by 3' AU-rich sequences. Nature (1988) 6.35
Detection of single base substitutions by ribonuclease cleavage at mismatches in RNA:DNA duplexes. Science (1985) 6.35
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The structure of the human zeta-globin gene and a closely linked, nearly identical pseudogene. Cell (1982) 4.13
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