A dodecapeptide comprising the extended chain-alpha 4 region of the restriction endonuclease EcoRI specifically binds to the EcoRI recognition site.

PubWeight™: 0.75‹?›

🔗 View Article (PMID 7890621)

Published in J Biol Chem on March 10, 1995

Authors

A Jeltsch1, J Alves, C Urbanke, G Maass, H Eckstein, Z Lianshan, E Bayer, A Pingoud

Author Affiliations

1: Institut für Biochemie, Justus-Liebig Universität, Giessen, Germany.

Articles by these authors

Synthesis and characterization of an octanucleotide containing the EcoRI recognition sequence with a phosphorothioate group at the cleavage site. Biochemistry (1984) 3.03

[Metabolic products of microorganisms. 98. Phosphinothricin and phosphinothricyl-alanyl-analine]. Helv Chim Acta (1972) 2.99

The conformational transitions in yeast tRNAPhe as studied with tRNAPhe fragments. Eur J Biochem (1973) 2.26

Preventing the spread of vancomycin-resistant enterococci in a long-term care facility. J Am Geriatr Soc (2000) 2.00

Effect of 4-quinolones and novobiocin on calf thymus DNA polymerase alpha primase complex, topoisomerases I and II, and growth of mammalian lymphoblasts. Antimicrob Agents Chemother (1986) 1.83

Horizontal gene transfer contributes to the wide distribution and evolution of type II restriction-modification systems. J Mol Evol (1996) 1.82

In vitro and in vivo function of the C-terminus of Escherichia coli single-stranded DNA binding protein. Nucleic Acids Res (1996) 1.77

Crystal structure of human mitochondrial single-stranded DNA binding protein at 2.4 A resolution. Nat Struct Biol (1997) 1.76

The interaction of the EcoRI restriction endonuclease with its substrate. A physico-chemical study employing natural and synthetic oligonucleotides and polynucleotides. Eur J Biochem (1980) 1.75

Culture and characteristics of hormone-responsive neuroblastoma X glioma hybrid cells. Methods Enzymol (1985) 1.70

Bcl-XL protects pancreatic adenocarcinoma cells against CD95- and TRAIL-receptor-mediated apoptosis. Oncogene (2000) 1.69

Influence of DNA target melting behavior on real-time PCR quantification. Clin Chem (2000) 1.66

A site-directed mutagenesis study to identify amino acid residues involved in the catalytic function of the restriction endonuclease EcoRV. Biochemistry (1992) 1.62

Interaction of E. coli single-stranded DNA binding protein (SSB) with exonuclease I. The carboxy-terminus of SSB is the recognition site for the nuclease. Biol Chem (2000) 1.57

Infectious foot-and-mouth disease virus derived from a cloned full-length cDNA. J Virol (1990) 1.54

Linear diffusion of restriction endonucleases on DNA. J Biol Chem (1985) 1.54

Ternary complex formation between elongation factor Tu, GTP and aminoacyl-tRNA: an equilibrium study. Eur J Biochem (1977) 1.50

Pausing of the restriction endonuclease EcoRI during linear diffusion on DNA. Biochemistry (1994) 1.46

The Cfr10I restriction enzyme is functional as a tetramer. J Mol Biol (1999) 1.46

Increased rate of lupus flare during pregnancy and the puerperium: a prospective study of 78 pregnancies. Br J Rheumatol (1996) 1.44

Single-stranded-DNA-binding proteins from human mitochondria and Escherichia coli have analogous physicochemical properties. Eur J Biochem (1994) 1.41

[Perinatal psychohygienics -- importance and chance of a primary preventive medicine by the gynaecologist (author's transl)]. Z Geburtshilfe Perinatol (1975) 1.40

[Usefulness of intracoronary therapy with progenitor cells in patients with dilated cardiomyopathy: Bridge or alternative to heart transplantation?]. An Pediatr (Barc) (2011) 1.40

Kinetic characterization of linear diffusion of the restriction endonuclease EcoRV on DNA. Biochemistry (1998) 1.39

Insolubilized biotin for the purification of avidin. Methods Enzymol (1974) 1.36

Role of thymidine residues in DNA recognition by the EcoRI and EcoRV restriction endonucleases. Nucleic Acids Res (1986) 1.34

Linear diffusion of the restriction endonuclease EcoRV on DNA is essential for the in vivo function of the enzyme. EMBO J (1996) 1.34

The stereochemical course of the restriction endonuclease EcoRI-catalyzed reaction. J Biol Chem (1984) 1.33

Isolation and identification of enteric adenoviruses. J Med Virol (1983) 1.32

Site-directed mutagenesis studies with EcoRV restriction endonuclease to identify regions involved in recognition and catalysis. Biochemistry (1991) 1.32

Substrate-assisted catalysis in the cleavage of DNA by the EcoRI and EcoRV restriction enzymes. Proc Natl Acad Sci U S A (1993) 1.31

Accuracy of the EcoRI restriction endonuclease: binding and cleavage studies with oligodeoxynucleotide substrates containing degenerate recognition sequences. Biochemistry (1990) 1.31

The influence of sequences adjacent to the recognition site on the cleavage of oligodeoxynucleotides by the EcoRI endonuclease. Eur J Biochem (1984) 1.30

Equivalent and non-equivalent binding sites for tRNA on aminoacyl-tRNA synthetases. Eur J Biochem (1975) 1.29

Mg2+ confers DNA binding specificity to the EcoRV restriction endonuclease. Biochemistry (1992) 1.27

Inhibition of Eco RI action by polynucleotides. A characterization of the non-specific binding of the enzyme to DNA. Nucleic Acids Res (1980) 1.25

Prediction of progressive liver fibrosis in hepatitis C infection by serum and tissue levels of transforming growth factor-beta. J Viral Hepat (2001) 1.23

The Fas-induced apoptosis analyzed by high throughput proteome analysis. J Biol Chem (2000) 1.20

Direct analysis of polymerase chain reaction products using enzyme-linked immunosorbent assay techniques. Anal Biochem (1991) 1.20

A mechanistic view of mitochondrial death decision pores. Braz J Med Biol Res (2007) 1.19

Evidence for an evolutionary relationship among type-II restriction endonucleases. Gene (1995) 1.19

Sau3AI, a monomeric type II restriction endonuclease that dimerizes on the DNA and thereby induces DNA loops. J Biol Chem (2001) 1.18

Psoralen treatment of adenovirus particles eliminates virus replication and transcription while maintaining the endosomolytic activity of the virus capsid. Virology (1994) 1.16

A fast and accurate enzyme-linked immunosorbent assay for the determination of the DNA cleavage activity of restriction endonucleases. Anal Biochem (1993) 1.16

Cross-linking of bromodeoxyuridine-substituted oligonucleotides to the EcoRI and EcoRV restriction endonucleases. Eur J Biochem (1986) 1.16

Kinetics of conformational changes in tRNA Phe (yeast) as studied by the fluorescence of the Y-base and of formycin substituted for the 3'-terminal adenine. Biophys Chem (1975) 1.16

Incorporation of hexose nucleoside analogues into oligonucleotides: synthesis, base-pairing properties and enzymatic stability. Nucleic Acids Res (1992) 1.16

The monomeric homing endonuclease PI-SceI has two catalytic centres for cleavage of the two strands of its DNA substrate. EMBO J (1999) 1.15

A similar active site for non-specific and specific endonucleases. Nat Struct Biol (1999) 1.15

Differential expression of NF-kappaB pathway genes among peripheral T-cell lymphomas. Leukemia (2005) 1.14

[Experimental infection of cercopithecus aethiops with the agent of the Frankfut-Marburg syndrome (FMS)]. Z Med Mikrobiol Immunol (1968) 1.14

A comparison of the structural requirements for DNA cleavage by the isoschizomers HaeIII, BspRI and BsuRI. Eur J Biochem (1985) 1.14

On the catalytic mechanism of EcoRI and EcoRV. A detailed proposal based on biochemical results, structural data and molecular modelling. FEBS Lett (1992) 1.14

Analysis of the mechanism of the Serratia nuclease using site-directed mutagenesis. Nucleic Acids Res (1996) 1.13

[13C-NMR chemical shifts of amino acids and peptides]. Z Naturforsch B (1971) 1.13

Distinct steps in the specific binding of tRNA to aminoacyl-tRNA synthetase. Temperature-jump studies on the serine-specific system from yeast and the tyrosine-specific system from Escherichia coli. Eur J Biochem (1976) 1.11

Introduction of asymmetry in the naturally symmetric restriction endonuclease EcoRV to investigate intersubunit communication in the homodimeric protein. Proc Natl Acad Sci U S A (1996) 1.10

A common core for binding single-stranded DNA: structural comparison of the single-stranded DNA-binding proteins (SSB) from E. coli and human mitochondria. FEBS Lett (1997) 1.10

Site directed mutagenesis experiments suggest that Glu 111, Glu 144 and Arg 145 are essential for endonucleolytic activity of EcoRI. Nucleic Acids Res (1986) 1.10

Transient cleavage kinetics of the Eco RI restriction endonuclease measured in a pulsed quench-flow apparatus: enzyme concentration-dependent activity change. Nucleic Acids Res (1981) 1.09

The elongation factor Tu binds aminoacyl-tRNA in the presence of GDP. J Biol Chem (1982) 1.09

Analysis of the recognition mechanism involved in the EcoRV catalyzed cleavage of DNA using modified oligodeoxynucleotides. Nucleic Acids Res (1988) 1.08

The coagulation/fibrinolysis balance in systemic sclerosis: evidence for a haematological stress syndrome. Br J Rheumatol (1997) 1.07

Mechanism of discrimination between cognate and non-cognate tRNAs by phenylalanyl-tRNA synthetase from yeast. Eur J Biochem (1976) 1.06

On the possibilities and limitations of rational protein design to expand the specificity of restriction enzymes: a case study employing EcoRV as the target. Protein Eng (2000) 1.06

Influence of the incorporation of (S)-9-(3,4-dihydroxybutyl)adenine on the enzymatic stability and base-pairing properties of oligodeoxynucleotides. Nucleic Acids Res (1991) 1.05

The effect of several nucleic acid binding drugs on the cleavage of d(GGAATTCC) and pBR 322 by the Eco RI restriction endonuclease. Nucleic Acids Res (1981) 1.05

Noncyclic crown-type polyethers, pyridinophane cryptands, and their alkali metal ion complexes: synthesis, complex stability, and kinetics. J Am Chem Soc (1977) 1.04

Cystic fibrosis with three mutations in the cystic fibrosis transmembrane conductance regulator gene. Hum Genet (1991) 1.03

The binding of ethidium bromide to different conformations of tRNA. Unfolding of tertiary structure. Eur J Biochem (1973) 1.03

Binding, bending and cleavage of DNA substrates by the homing endonuclease Pl-SceI. Nucleic Acids Res (1996) 1.03

Tertiary structure of tRNAPhe (yeast): kinetics and electrostatic repulsion. Eur J Biochem (1975) 1.03